Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A90.0% 855.9 / 99.1 259coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (12/14);  new base A (105/128);  total (117/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 271 total aligned reads displayed.

CCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAAT  >  CP009273/2108071‑2108363
                                                                                                                                                     |                                                                                                                                                
ccTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑a                                                                                                                                                 <  1:5051725/150‑1 (MQ=255)
      tcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaa                                                                                                                                           >  2:281636/1‑150 (MQ=255)
       cattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaa                                                                                                                                          >  1:4338670/1‑149 (MQ=255)
        attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaat                                                                                                                                         <  2:3168541/150‑3 (MQ=255)
         ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaatg                                                                                                                                        <  1:151909/150‑4 (MQ=255)
         ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaatg                                                                                                                                        >  1:2955886/1‑147 (MQ=255)
          tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaatgt                                                                                                                                       >  1:422520/1‑146 (MQ=255)
             ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGc                                                                                                                                     <  2:3247973/150‑1 (MQ=255)
              cGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCgg                                                                                                                                   >  2:780444/1‑150 (MQ=255)
                cATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGt                                                                                                                                  >  2:1590348/1‑150 (MQ=255)
                   gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTttg                                                                                                                               >  1:2946634/1‑150 (MQ=255)
                       aTTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTtgttgt                                                                                                                           >  1:4474168/1‑150 (MQ=255)
                        ttttCAAGAGACAGTAGTGTTACTTTTTTCGCCATAAACACACCACGATCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATAACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTtgtg                                                                                                                          <  1:2638116/150‑1 (MQ=255)
                          tGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTa                                                                                                                        <  2:371412/150‑1 (MQ=255)
                            cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAAc                                                                                                                      >  1:3479814/1‑150 (MQ=255)
                             aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACt                                                                                                                     <  2:2297349/150‑1 (MQ=255)
                              agagACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTc                                                                                                                    <  1:3728790/150‑1 (MQ=255)
                               gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTcc                                                                                                                   <  2:1268775/150‑1 (MQ=255)
                                   cagcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCg                                                                                                               <  1:3298912/150‑1 (MQ=255)
                                      cagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacg                                                                                                            >  2:79491/1‑150 (MQ=255)
                                      cagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacg                                                                                                            <  1:25329/150‑1 (MQ=255)
                                       agTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                           >  1:2080265/1‑150 (MQ=255)
                                        gtgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgca                                                                                                          <  2:2887572/150‑1 (MQ=255)
                                           tttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacgc                                                                                                       >  2:2846780/1‑150 (MQ=255)
                                               ttttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCa                                                                                                   >  1:5018615/1‑150 (MQ=255)
                                                tttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAt                                                                                                  <  2:5017289/150‑1 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                <  1:3139758/150‑1 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                >  2:2004842/1‑150 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                >  2:4560153/1‑150 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtctc                                                                                               >  2:1376279/1‑150 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtctc                                                                                               <  1:4121877/150‑1 (MQ=255)
                                                   ttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtctc                                                                                               >  1:3703109/1‑150 (MQ=255)
                                                     cGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCaa                                                                                             >  1:473290/1‑150 (MQ=255)
                                                          tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtta                                                                                        <  1:1657511/150‑1 (MQ=255)
                                                          tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtta                                                                                        <  1:2141853/150‑1 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                      <  1:1951800/150‑1 (MQ=255)
                                                             aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                     <  1:1044115/150‑1 (MQ=255)
                                                             aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                     >  1:111744/1‑150 (MQ=255)
                                                                caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                  >  2:1391533/1‑150 (MQ=255)
                                                                 accacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcg                                                                                 <  2:1096256/150‑1 (MQ=255)
                                                                  ccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcgc                                                                                >  2:1856582/1‑150 (MQ=255)
                                                                   cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                               <  2:2246158/150‑1 (MQ=255)
                                                                   cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                               >  1:4944585/1‑150 (MQ=255)
                                                                    acGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCca                                                                              >  1:1148421/1‑150 (MQ=255)
                                                                     cGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcac                                                                             <  2:3010129/150‑1 (MQ=255)
                                                                      gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcaca                                                                            <  2:726304/150‑1 (MQ=255)
                                                                      gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGGTGGTTGTGTAACTCCCGCGACGCACGCGTCATCTCAATGTTCCTGAGCGCcaca                                                                            >  2:4011400/1‑150 (MQ=255)
                                                                         cGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAg                                                                         >  1:3508655/1‑150 (MQ=255)
                                                                          gTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                        <  1:51061/150‑1 (MQ=255)
                                                                          gTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                        <  1:51195/150‑1 (MQ=255)
                                                                          gTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                        >  2:3853530/1‑150 (MQ=255)
                                                                           tCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGa                                                                       <  1:1780854/150‑1 (MQ=255)
                                                                            cTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaaa                                                                     <  2:733859/150‑1 (MQ=255)
                                                                            cTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaaa                                                                     <  2:4171673/150‑1 (MQ=255)
                                                                              ttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAAc                                                                    >  2:4381314/1‑150 (MQ=255)
                                                                                 cgcATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGc                                                                 >  2:1783153/1‑150 (MQ=255)
                                                                                    aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              <  2:4646540/150‑1 (MQ=255)
                                                                                    aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              <  2:531555/150‑1 (MQ=255)
                                                                                    aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              <  2:533784/150‑1 (MQ=255)
                                                                                    aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              >  1:4625856/1‑150 (MQ=255)
                                                                                     tGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                             <  2:1177230/150‑1 (MQ=255)
                                                                                      gTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                            >  1:3397893/1‑150 (MQ=255)
                                                                                        cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          <  2:356575/150‑1 (MQ=255)
                                                                                        cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          <  2:356498/150‑1 (MQ=255)
                                                                                        cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          >  2:1484711/1‑150 (MQ=255)
                                                                                          aaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggc                                                        >  2:1908911/1‑150 (MQ=255)
                                                                                            gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                     <  1:3971177/150‑1 (MQ=255)
                                                                                             ttCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                     <  2:1242231/150‑1 (MQ=255)
                                                                                                aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                  <  1:5083471/150‑1 (MQ=255)
                                                                                                aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                  <  1:2541028/150‑1 (MQ=255)
                                                                                                 tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                                 <  2:2334683/150‑1 (MQ=255)
                                                                                                    tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                              >  1:1431815/1‑150 (MQ=255)
                                                                                                       aTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                           <  2:3066859/150‑1 (MQ=255)
                                                                                                       aTGCGGGTCCAACTGAGGAGTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                           <  2:1634268/150‑1 (MQ=255)
                                                                                                        tGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGc                                          <  1:1237387/150‑1 (MQ=255)
                                                                                                         gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGcc                                         <  1:2582729/150‑1 (MQ=255)
                                                                                                         gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGcc                                         <  1:4428082/150‑1 (MQ=255)
                                                                                                               ccACCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCaa                                   <  1:4131684/150‑1 (MQ=255)
                                                                                                                  aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                <  1:755620/150‑1 (MQ=255)
                                                                                                                  aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                <  2:1526108/150‑1 (MQ=255)
                                                                                                                     gAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                             >  1:725825/1‑150 (MQ=255)
                                                                                                                     gAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                             >  1:1339119/1‑150 (MQ=255)
                                                                                                                     gAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                             >  2:3804942/1‑150 (MQ=255)
                                                                                                                        gATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGc                          <  1:2836178/150‑1 (MQ=255)
                                                                                                                           tCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                       <  1:3888453/150‑1 (MQ=255)
                                                                                                                           tCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                       <  2:4908781/150‑1 (MQ=255)
                                                                                                                                cATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                  >  1:2461695/1‑150 (MQ=255)
                                                                                                                                cATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                  >  2:254691/1‑150 (MQ=255)
                                                                                                                                   cacaTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCCATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtat               <  1:310286/150‑1 (MQ=255)
                                                                                                                                    acaTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt              <  2:4250793/150‑1 (MQ=255)
                                                                                                                                    acaTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt              >  1:539915/1‑150 (MQ=255)
                                                                                                                                      aTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat            >  2:2101418/1‑150 (MQ=255)
                                                                                                                                       tttatatTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc           >  1:910962/4‑150 (MQ=255)
                                                                                                                                           tatTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGt       >  2:1826901/1‑150 (MQ=255)
                                                                                                                                             tttCTCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACTCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAATCAAGATGAGTATTATCCAGTTg     <  2:1002015/150‑1 (MQ=255)
                                                                                                                                             tttCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTg     >  2:4048111/1‑150 (MQ=255)
                                                                                                                                              ttCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa    <  1:1011600/150‑1 (MQ=255)
                                                                                                                                               tCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGaa   >  1:3349288/1‑150 (MQ=255)
                                                                                                                                               tCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGaa   <  2:5132678/150‑1 (MQ=255)
                                                                                                                                                cGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAat  >  1:1584996/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAAT  >  CP009273/2108071‑2108363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A