Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A97.2% 765.3 / 13.9 216coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base A (101/109);  total (104/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 234 total aligned reads displayed.

TCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAAT  >  CP009273/2108070‑2108363
                                                                                                                                                      |                                                                                                                                                 
tCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAt                                                                                                                                                    <  1:2032968/150‑1 (MQ=255)
  cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa                                                                                                                                                 >  2:2424507/1‑150 (MQ=255)
    ttttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑acaa                                                                                                                                               >  2:2183408/1‑150 (MQ=255)
     tttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa                                                                                                                                              >  2:4016085/1‑150 (MQ=255)
      ttcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa‑a                                                                                                                                            >  1:768392/1‑150 (MQ=255)
         attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa‑aaat                                                                                                                                         <  1:2909326/150‑3 (MQ=255)
         attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa‑aaat                                                                                                                                         <  2:280231/150‑3 (MQ=255)
          ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa‑aaatg                                                                                                                                        <  2:3657028/150‑4 (MQ=255)
           tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa‑aaatgt                                                                                                                                       <  2:2008342/150‑5 (MQ=255)
            caCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa‑aaatgtt                                                                                                                                      >  2:2269124/1‑145 (MQ=255)
              ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGc                                                                                                                                     <  1:1028660/150‑1 (MQ=255)
                gCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCgg                                                                                                                                   <  1:873252/150‑1 (MQ=255)
                  aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGtt                                                                                                                                 <  2:2522234/150‑1 (MQ=255)
                  aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGtt                                                                                                                                 <  2:2858540/150‑1 (MQ=255)
                  aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAA‑AATGTTGCGGttt                                                                                                                                <  1:3107701/150‑1 (MQ=255)
                    gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTttg                                                                                                                               <  2:2229485/150‑1 (MQ=255)
                      aCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTttgtt                                                                                                                             >  1:1953431/1‑150 (MQ=255)
                      aCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGATCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTttgtt                                                                                                                             >  2:820557/1‑150 (MQ=255)
                         tttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTtgtg                                                                                                                          <  2:2937009/150‑1 (MQ=255)
                              aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTATTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAATAATGTTGCGGTTTGTTGTGTAACt                                                                                                                     <  2:1932618/150‑1 (MQ=255)
                               agagACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTc                                                                                                                    >  1:2356890/1‑150 (MQ=255)
                               agagACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTGTGTTGTGTAACTc                                                                                                                    >  2:4093926/1‑150 (MQ=255)
                                 agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCa                                                                                                                  >  1:1721328/1‑150 (MQ=255)
                                   aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGc                                                                                                                >  1:3763886/1‑150 (MQ=255)
                                     agcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGa                                                                                                              >  2:183703/1‑150 (MQ=255)
                                     agcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGa                                                                                                              >  2:183724/1‑150 (MQ=255)
                                      gcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGac                                                                                                             <  2:1080662/150‑1 (MQ=255)
                                        agTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                           >  2:1681142/1‑150 (MQ=255)
                                        agTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                           <  2:722485/150‑1 (MQ=255)
                                         gtgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgca                                                                                                          >  1:2459541/1‑150 (MQ=255)
                                         gtgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgca                                                                                                          <  2:1218570/150‑1 (MQ=255)
                                         gtgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgca                                                                                                          >  1:850938/1‑150 (MQ=255)
                                          tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcac                                                                                                         >  1:2774447/1‑150 (MQ=255)
                                           gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                        <  1:1671982/150‑1 (MQ=255)
                                           gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                        <  1:548664/150‑1 (MQ=255)
                                           gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                        >  1:2238442/1‑150 (MQ=255)
                                             ttCTTTTTTCGCCCTAACAACACCCCGAACGTCTTTTCGCTTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGTGGATTCCTGCATCTCCTTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCCGCGACGCACGCt                                                                                                      <  2:1539857/150‑1 (MQ=255)
                                                      cGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCaa                                                                                             >  1:3961310/1‑150 (MQ=255)
                                                       gCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAt                                                                                            <  2:517143/150‑1 (MQ=255)
                                                       gCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAt                                                                                            <  1:1828472/150‑1 (MQ=255)
                                                         cATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgt                                                                                          >  2:1529309/1‑150 (MQ=255)
                                                             aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGTATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                      <  2:1128666/150‑1 (MQ=255)
                                                               aaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                    <  1:2010506/150‑1 (MQ=255)
                                                                 caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                  >  2:298069/1‑150 (MQ=255)
                                                                 caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                  >  2:3198605/1‑150 (MQ=255)
                                                                   ccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcgc                                                                                >  2:57442/1‑150 (MQ=255)
                                                                    cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                               <  2:324330/150‑1 (MQ=255)
                                                                       gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcaca                                                                            >  1:214994/1‑150 (MQ=255)
                                                                        aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                           >  2:1408474/1‑150 (MQ=255)
                                                                        aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                           >  1:527480/1‑150 (MQ=255)
                                                                         aCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaaa                                                                          <  1:3182977/150‑1 (MQ=255)
                                                                                     aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGACACAGCAtt                                                              >  2:3631226/1‑150 (MQ=255)
                                                                                     aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              <  1:2558601/150‑1 (MQ=255)
                                                                                     aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              >  1:307171/1‑150 (MQ=255)
                                                                                      tGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                             <  2:2511158/150‑1 (MQ=255)
                                                                                        tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTccc                                                           <  2:3472816/150‑1 (MQ=255)
                                                                                         cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          <  1:1592778/150‑1 (MQ=255)
                                                                                            aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                       <  1:242845/150‑1 (MQ=255)
                                                                                              ttCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                     <  2:625500/150‑1 (MQ=255)
                                                                                                cATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                   >  1:302936/1‑150 (MQ=255)
                                                                                                cATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGACACAAAGGAAACAGCATTCCCGGCGgaga                                                   <  1:1059706/150‑1 (MQ=255)
                                                                                                  tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                                 <  1:436185/150‑1 (MQ=255)
                                                                                                   gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAttt                                                <  1:1658426/150‑1 (MQ=255)
                                                                                                   gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAttt                                                <  1:3279240/150‑1 (MQ=255)
                                                                                                   gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAttt                                                <  1:4098360/150‑1 (MQ=255)
                                                                                                    cTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                               <  1:4143923/150‑1 (MQ=255)
                                                                                                    cTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                               <  1:1206252/150‑1 (MQ=255)
                                                                                                     tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                              >  1:3758209/1‑150 (MQ=255)
                                                                                                      cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGaa                                             <  1:4060940/150‑1 (MQ=255)
                                                                                                      cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGaa                                             >  2:73004/1‑150 (MQ=255)
                                                                                                      cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTGGaa                                             >  1:2991/1‑150 (MQ=255)
                                                                                                       aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTAATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAGTTGAAt                                            >  1:389802/1‑150 (MQ=255)
                                                                                                           cGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCcg                                        <  1:3489399/150‑1 (MQ=255)
                                                                                                               tCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCa                                    <  1:1291381/150‑1 (MQ=255)
                                                                                                                 cAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                  >  1:3727607/1‑150 (MQ=255)
                                                                                                                 cAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                  <  1:92222/150‑1 (MQ=255)
                                                                                                                 cAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                  >  1:33284/1‑150 (MQ=255)
                                                                                                                   aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                >  2:1286143/1‑150 (MQ=255)
                                                                                                                   aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                <  2:2398101/150‑1 (MQ=255)
                                                                                                                   aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                >  1:4072176/1‑150 (MQ=255)
                                                                                                                    cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                               <  1:2041835/150‑1 (MQ=255)
                                                                                                                     tGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                              >  2:670745/1‑150 (MQ=255)
                                                                                                                            tCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACTCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                       <  1:2967014/150‑1 (MQ=255)
                                                                                                                            tCCTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                       <  1:3300682/150‑1 (MQ=255)
                                                                                                                             ccTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGa                      >  1:3670698/1‑150 (MQ=255)
                                                                                                                              cTGCATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGAt                     >  2:2435039/1‑150 (MQ=255)
                                                                                                                                 cATCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                  <  2:2630064/150‑1 (MQ=255)
                                                                                                                                  aTCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt                 >  1:1414146/1‑150 (MQ=255)
                                                                                                                                  aTCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt                 >  1:2119974/1‑150 (MQ=255)
                                                                                                                                   tCACATTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGta                <  2:1495713/150‑1 (MQ=255)
                                                                                                                                      caTTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta             <  2:3898754/150‑1 (MQ=255)
                                                                                                                                      caTTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta             <  2:3691074/150‑1 (MQ=255)
                                                                                                                                       aTTGATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat            >  1:1010706/1‑150 (MQ=255)
                                                                                                                                          gATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa         <  2:1744063/150‑1 (MQ=255)
                                                                                                                                          gATATTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa         <  2:1411338/150‑1 (MQ=255)
                                                                                                                                            tatTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGt       >  2:549295/1‑150 (MQ=255)
                                                                                                                                            tatTTCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGt       <  1:2476738/150‑1 (MQ=255)
                                                                                                                                               ttCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa    >  2:1756671/1‑150 (MQ=255)
                                                                                                                                                tCGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGaa   <  2:2291438/150‑1 (MQ=255)
                                                                                                                                                 cGCATAAAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAat  <  1:4005184/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
TCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAA‑AATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAAT  >  CP009273/2108070‑2108363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A