Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A99.6% 813.5 / ‑2.9 243coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (126/116);  total (127/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 272 total aligned reads displayed.

CTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108072‑2108361
                                                                                                                                                    |                                                                                                                                              
   tttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATATAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaa                                                                                                                                             >  1:1669918/1‑148 (MQ=255)
   tttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaa                                                                                                                                             >  1:3477341/1‑148 (MQ=255)
    ttcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaa                                                                                                                                            <  1:1546893/148‑1 (MQ=255)
     tcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa                                                                                                                                           <  1:3158168/148‑1 (MQ=255)
     tcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaa                                                                                                                                           <  1:148911/148‑1 (MQ=255)
        ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaa                                                                                                                                        <  2:2475725/148‑2 (MQ=255)
         tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaat                                                                                                                                       <  2:2283424/148‑3 (MQ=255)
         tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaat                                                                                                                                       <  2:2283460/148‑3 (MQ=255)
            ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaatgtt                                                                                                                                    <  1:1315721/148‑6 (MQ=255)
            ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaatgtt                                                                                                                                    >  2:2708169/1‑143 (MQ=255)
            ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaaaatgtt                                                                                                                                    >  1:1890454/1‑143 (MQ=255)
                aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCgg                                                                                                                                 >  1:116143/1‑148 (MQ=255)
                  gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGtt                                                                                                                               >  2:86889/1‑148 (MQ=255)
                     cATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGAGGTttgt                                                                                                                            >  2:2411217/1‑148 (MQ=255)
                       tttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTttgttg                                                                                                                          <  1:1429281/148‑1 (MQ=255)
                       tttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTttgttg                                                                                                                          <  2:403722/148‑1 (MQ=255)
                        ttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTtgttgt                                                                                                                         >  2:2204450/1‑148 (MQ=255)
                          gCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTgtgt                                                                                                                       <  2:3121186/148‑1 (MQ=255)
                           cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTa                                                                                                                      >  1:941459/1‑148 (MQ=255)
                           cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTa                                                                                                                      <  1:483558/148‑1 (MQ=255)
                               agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTc                                                                                                                  <  2:1629396/148‑1 (MQ=255)
                                 aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCa                                                                                                                >  1:45211/1‑148 (MQ=255)
                                  cagcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                               <  2:381157/148‑1 (MQ=255)
                                        tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                         >  1:2577245/1‑148 (MQ=255)
                                           ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  2:7104/1‑148 (MQ=255)
                                           ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  2:1363609/1‑148 (MQ=255)
                                           ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      <  1:2770304/148‑1 (MQ=255)
                                            tCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacgc                                                                                                     >  1:513754/1‑148 (MQ=255)
                                             cTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCt                                                                                                    >  1:1501079/1‑148 (MQ=255)
                                              ttttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCtt                                                                                                   <  1:2426449/148‑1 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         <  1:2154569/148‑1 (MQ=255)
                                                         tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgt                                                                                        <  2:1386988/148‑1 (MQ=255)
                                                         tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgt                                                                                        >  2:1886752/1‑148 (MQ=255)
                                                          aaaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTACCTCCAGCGACGCACGCTTCATCTCAATgtt                                                                                       >  1:3574381/1‑148 (MQ=255)
                                                          aaaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtt                                                                                       >  1:3574610/1‑148 (MQ=255)
                                                          aaaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtt                                                                                       >  1:1715198/1‑148 (MQ=255)
                                                           aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtta                                                                                      <  1:2638111/148‑1 (MQ=255)
                                                           aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtta                                                                                      >  1:689049/1‑148 (MQ=255)
                                                           aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAAATCCAGCGACGCACGCTTCATCTCAATgtta                                                                                      >  1:1891760/1‑148 (MQ=255)
                                                            aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttag                                                                                     <  2:1258036/148‑1 (MQ=255)
                                                             aaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                    >  1:3360218/1‑148 (MQ=255)
                                                             aaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                    <  1:2884176/148‑1 (MQ=255)
                                                              acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   <  2:2878940/148‑1 (MQ=255)
                                                               caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  2:2131856/1‑148 (MQ=255)
                                                                 ccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                >  2:640729/1‑148 (MQ=255)
                                                                  cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcg                                                                               >  2:1586737/1‑148 (MQ=255)
                                                                    cGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                             >  1:3781129/1‑148 (MQ=255)
                                                                    cGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                             >  1:3781891/1‑148 (MQ=255)
                                                                      aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcac                                                                           >  1:843378/1‑148 (MQ=255)
                                                                       aCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcaca                                                                          >  2:930236/1‑148 (MQ=255)
                                                                        cGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                         >  2:2713781/1‑148 (MQ=255)
                                                                         gTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaaa                                                                        <  1:3745143/148‑1 (MQ=255)
                                                                            tttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGa                                                                     >  2:3433961/1‑148 (MQ=255)
                                                                             ttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaa                                                                    >  2:3185242/1‑148 (MQ=255)
                                                                             ttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaa                                                                    <  2:800449/148‑1 (MQ=255)
                                                                                   aTGTCTAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCa                                                              <  2:2436897/148‑1 (MQ=255)
                                                                                       cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          >  2:3660799/1‑148 (MQ=255)
                                                                                         aaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                        >  1:2594670/1‑148 (MQ=255)
                                                                                          aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcgg                                                       >  1:1731391/1‑148 (MQ=255)
                                                                                           gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGCTAGTTAGCGCCACAAAGGAAACAGCATTCCcggc                                                      >  1:1339951/1‑148 (MQ=255)
                                                                                            ttCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                     >  2:980318/1‑148 (MQ=255)
                                                                                             tCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcgg                                                    >  2:1454208/1‑148 (MQ=255)
                                                                                               aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgag                                                  <  2:2001251/148‑1 (MQ=255)
                                                                                               aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgag                                                  <  2:2523578/148‑1 (MQ=255)
                                                                                                tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 >  2:490368/1‑148 (MQ=255)
                                                                                                 gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                <  1:2488340/148‑1 (MQ=255)
                                                                                                 gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                <  2:2688578/148‑1 (MQ=255)
                                                                                                    cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             >  1:3030076/1‑148 (MQ=255)
                                                                                                    cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             >  1:3357243/1‑148 (MQ=255)
                                                                                                     aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                            <  2:3765596/148‑1 (MQ=255)
                                                                                                     aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                            <  1:2239848/148‑1 (MQ=255)
                                                                                                      aTGCGGATCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGaa                                           <  2:1952367/148‑1 (MQ=255)
                                                                                                       tGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                          >  2:1499863/1‑148 (MQ=255)
                                                                                                       tGCGGGTACAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                          >  2:3380911/1‑148 (MQ=255)
                                                                                                        gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTTTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                         <  1:1062497/148‑1 (MQ=255)
                                                                                                        gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                         <  1:3105450/148‑1 (MQ=255)
                                                                                                           ggTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCcg                                      >  1:3230125/1‑148 (MQ=255)
                                                                                                              ccAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCgcgc                                   <  2:3447221/148‑1 (MQ=255)
                                                                                                                   tGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                              >  1:2989177/1‑148 (MQ=255)
                                                                                                                   tGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                              <  1:2015309/148‑1 (MQ=255)
                                                                                                                      ggATTCCTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                           <  2:1040215/148‑1 (MQ=255)
                                                                                                                           ccTGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCaa                      >  1:3382759/1‑148 (MQ=255)
                                                                                                                             tGCATCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGa                    <  2:1863339/148‑1 (MQ=255)
                                                                                                                                 tCACATTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                >  1:29267/1‑148 (MQ=255)
                                                                                                                                  cacaTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               <  1:1859547/148‑1 (MQ=255)
                                                                                                                                  cacaTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               <  1:209334/148‑1 (MQ=255)
                                                                                                                                   acaTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGta              <  1:1364027/148‑1 (MQ=255)
                                                                                                                                    caTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtat             >  1:1497958/1‑148 (MQ=255)
                                                                                                                                    caTTGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtat             >  1:1019175/1‑148 (MQ=255)
                                                                                                                                      ttGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           >  1:1605353/1‑148 (MQ=255)
                                                                                                                                       tGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          >  2:3494623/1‑148 (MQ=255)
                                                                                                                                       tGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          <  1:2716839/148‑1 (MQ=255)
                                                                                                                                       tGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          >  2:3039080/1‑148 (MQ=255)
                                                                                                                                       tGATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          >  1:57239/1‑148 (MQ=255)
                                                                                                                                        gATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc         <  2:464621/148‑1 (MQ=255)
                                                                                                                                        gATATTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc         <  2:1469477/148‑1 (MQ=255)
                                                                                                                                         atatTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        <  1:3433761/148‑1 (MQ=255)
                                                                                                                                           atTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg      >  1:156670/1‑148 (MQ=255)
                                                                                                                                           atTTCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg      <  2:176723/148‑1 (MQ=255)
                                                                                                                                            tttCGCATAAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGt     <  1:306995/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108072‑2108361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A