Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A85.4% 702.1 / 118.1 213coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (14/17);  new base A (94/88);  total (108/105)
Fisher's exact test for biased strand distribution p-value = 5.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 238 total aligned reads displayed.

TCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATA  >  CP009273/2108070‑2108364
                                                                                                                                                      |                                                                                                                                                   
tCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAt                                                                                                                                                      <  2:3653330/150‑1 (MQ=255)
 ccTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑a                                                                                                                                                    >  1:1196737/1‑150 (MQ=255)
 ccTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑a                                                                                                                                                    <  1:3290250/150‑1 (MQ=255)
 ccTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑a                                                                                                                                                    <  1:1624525/150‑1 (MQ=255)
  cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa                                                                                                                                                   >  2:3641224/1‑150 (MQ=255)
      ttcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaa                                                                                                                                               <  1:3102728/150‑1 (MQ=255)
          ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaa‑aatg                                                                                                                                          <  2:2801060/150‑4 (MQ=255)
           tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaa‑aatgt                                                                                                                                         >  1:589641/1‑146 (MQ=255)
            caCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaaaa‑aatgtt                                                                                                                                        <  2:3968718/150‑6 (MQ=255)
              ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGc                                                                                                                                      <  1:3655499/150‑1 (MQ=255)
                gCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCgg                                                                                                                                    <  1:215478/150‑1 (MQ=255)
                gCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑ACTGTTGCgg                                                                                                                                    >  2:1589007/1‑150 (MQ=255)
                gCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGt                                                                                                                                   <  2:4292988/150‑1 (MQ=255)
                 cATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGt                                                                                                                                   >  2:440465/1‑150 (MQ=255)
                    gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTttg                                                                                                                                <  1:4249314/150‑1 (MQ=255)
                     aaCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTttgt                                                                                                                               <  2:3157478/150‑1 (MQ=255)
                     aaCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTttgt                                                                                                                               <  1:4145204/150‑1 (MQ=255)
                      aCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTttgtt                                                                                                                              >  1:2985298/1‑150 (MQ=255)
                       cATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTtgttgt                                                                                                                            >  2:3756156/1‑150 (MQ=255)
                            gCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTaa                                                                                                                        >  1:996936/1‑150 (MQ=255)
                             cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAAc                                                                                                                       <  2:2765796/150‑1 (MQ=255)
                              aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACt                                                                                                                      >  2:1738476/1‑150 (MQ=255)
                              aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAATA‑TGTTGCGGTTTGTTGTGTAACt                                                                                                                      >  1:343476/1‑150 (MQ=255)
                                gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTcc                                                                                                                    <  2:3550199/150‑1 (MQ=255)
                                gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCa                                                                                                                   <  1:362559/150‑1 (MQ=255)
                                 agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCa                                                                                                                   >  1:3423747/1‑150 (MQ=255)
                                  gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                                  <  1:1341276/150‑1 (MQ=255)
                                  gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                                  <  1:4062008/150‑1 (MQ=255)
                                  gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                                  <  1:2130330/150‑1 (MQ=255)
                                   aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGc                                                                                                                 <  1:2433270/150‑1 (MQ=255)
                                     agcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGa                                                                                                               >  2:2238878/1‑150 (MQ=255)
                                        agTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                            >  1:3916929/1‑150 (MQ=255)
                                          tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacgcac                                                                                                          <  2:1854388/150‑1 (MQ=255)
                                           gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                         >  1:1318305/1‑150 (MQ=255)
                                             ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCt                                                                                                       <  2:389717/150‑1 (MQ=255)
                                              tCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCtt                                                                                                      <  1:2878351/150‑1 (MQ=255)
                                              tCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCtt                                                                                                      <  1:2877403/150‑1 (MQ=255)
                                               cTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTc                                                                                                     >  2:3931812/1‑150 (MQ=255)
                                                   tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                 >  1:1970575/1‑150 (MQ=255)
                                                      cGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCaa                                                                                              >  2:948679/1‑150 (MQ=255)
                                                        ccATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                            >  1:2125351/1‑150 (MQ=255)
                                                              aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                      >  1:727533/1‑150 (MQ=255)
                                                                 caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                   <  2:1024626/150‑1 (MQ=255)
                                                                     acGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCca                                                                               <  2:3166076/150‑1 (MQ=255)
                                                                       gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                            >  2:2174207/1‑150 (MQ=255)
                                                                        aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                            <  1:1530162/150‑1 (MQ=255)
                                                                          cGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAg                                                                          >  2:1643812/1‑150 (MQ=255)
                                                                           gTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGa                                                                        <  2:2445745/150‑1 (MQ=255)
                                                                           gTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGa                                                                        <  1:940309/150‑1 (MQ=255)
                                                                               ttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAAc                                                                     >  1:124182/1‑150 (MQ=255)
                                                                                   gcATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCa                                                                 >  1:2565135/1‑150 (MQ=255)
                                                                                    cATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGAGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCaa                                                                >  1:3182715/1‑149 (MQ=255)
                                                                                       gTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                             >  2:426446/1‑150 (MQ=255)
                                                                                       gTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                             <  2:3674615/150‑1 (MQ=255)
                                                                                       gTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTccc                                                            <  2:61677/150‑1 (MQ=255)
                                                                                         cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                           >  1:4298290/1‑150 (MQ=255)
                                                                                            aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                        <  2:301329/150‑1 (MQ=255)
                                                                                            aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                        <  2:3469422/150‑1 (MQ=255)
                                                                                             gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGACACAGCATTCCcggcgg                                                       >  1:905510/1‑150 (MQ=255)
                                                                                             gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                      >  2:2977634/1‑150 (MQ=255)
                                                                                                cATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                    >  1:2963513/1‑150 (MQ=255)
                                                                                                cATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                    >  1:3705599/1‑150 (MQ=255)
                                                                                                 aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                   >  1:3824380/1‑150 (MQ=255)
                                                                                                  tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                                  >  2:1373987/1‑150 (MQ=255)
                                                                                                  tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                                  >  2:1372202/1‑150 (MQ=255)
                                                                                                    cTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                                >  1:3589053/1‑150 (MQ=255)
                                                                                                     tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                               >  2:1411256/1‑150 (MQ=255)
                                                                                                     tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                               >  2:3756853/1‑150 (MQ=255)
                                                                                                     tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                               <  1:1002325/150‑1 (MQ=255)
                                                                                                       aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                             <  2:318077/150‑1 (MQ=255)
                                                                                                           cGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCcg                                         <  1:1062060/150‑1 (MQ=255)
                                                                                                                   aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                 <  2:1230124/150‑1 (MQ=255)
                                                                                                                   aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                 <  2:1185500/150‑1 (MQ=255)
                                                                                                                    cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                                <  1:2176378/150‑1 (MQ=255)
                                                                                                                    cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                                <  2:4176368/150‑1 (MQ=255)
                                                                                                                    cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGGTGCGGTGTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCCATTta                                >  2:548949/1‑150 (MQ=255)
                                                                                                                      gAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                              >  2:2685998/1‑150 (MQ=255)
                                                                                                                       aGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAg                            <  2:49719/150‑1 (MQ=255)
                                                                                                                        ggATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAg                            <  1:4170854/150‑1 (MQ=255)
                                                                                                                         gATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGTAAACAGCATACCCGGCGGAGATTTGAATGACGCGCAATTTATAAGc                           >  1:3864321/1‑150 (MQ=255)
                                                                                                                         gATTCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGc                           >  1:3864709/1‑150 (MQ=255)
                                                                                                                           ttCCTGCATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCaa                         <  1:2612460/150‑1 (MQ=255)
                                                                                                                                 cATCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                   >  2:3950339/1‑150 (MQ=255)
                                                                                                                                   tCACATTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGta                 <  1:2480898/150‑1 (MQ=255)
                                                                                                                                     acaTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt               <  2:4136039/150‑1 (MQ=255)
                                                                                                                                     acaTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt               <  1:775196/150‑1 (MQ=255)
                                                                                                                                     acaTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt               <  1:1864671/150‑1 (MQ=255)
                                                                                                                                     acaTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt               <  1:2092022/150‑1 (MQ=255)
                                                                                                                                     acaTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt               <  1:3641224/150‑1 (MQ=255)
                                                                                                                                      caTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta              <  1:3924943/150‑1 (MQ=255)
                                                                                                                                       aTTGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat             <  1:2012644/150‑1 (MQ=255)
                                                                                                                                        ttGATATTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc            <  2:147827/150‑1 (MQ=255)
                                                                                                                                          gATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg         <  2:4486804/150‑1 (MQ=255)
                                                                                                                                           atatTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg         <  1:956723/150‑1 (MQ=255)
                                                                                                                                           atatTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg         >  1:2373165/1‑150 (MQ=255)
                                                                                                                                           atatTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg         <  1:2465154/150‑1 (MQ=255)
                                                                                                                                           atatTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg         >  1:3451825/1‑150 (MQ=255)
                                                                                                                                           atatTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg         >  1:917150/1‑150 (MQ=255)
                                                                                                                                             atTTCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGtt       >  2:846104/1‑150 (MQ=255)
                                                                                                                                                tCGCATAAAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGaa    <  2:639041/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAAAA‑A‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATA  >  CP009273/2108070‑2108364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A