Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A98.6% 1158.9 / 8.6 350coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base A (175/170);  total (178/172)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 381 total aligned reads displayed.

CCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAAT  >  CP009273/2108071‑2108363
                                                                                                                                                     |                                                                                                                                                 
 cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aa                                                                                                                                                <  2:3875882/150‑1 (MQ=255)
   ttttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaa                                                                                                                                              <  1:3934215/150‑1 (MQ=255)
   ttttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaa                                                                                                                                              <  1:229368/150‑1 (MQ=255)
        attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaaaaaat                                                                                                                                         >  1:3368352/1‑148 (MQ=255)
        attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaaaaaat                                                                                                                                         <  2:1828634/150‑3 (MQ=255)
        attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaaaaaat                                                                                                                                         <  2:461123/150‑3 (MQ=255)
        attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaaaaaat                                                                                                                                         <  2:4280203/150‑3 (MQ=255)
          tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaaaaaatgt                                                                                                                                       <  2:1057064/150‑5 (MQ=255)
          tcaCCGCATGAACATTTGCAAGAGACAGCAGTGGTTCTTTTTTCGCCATAAAAACACCACGATCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑aaaaaaaatgt                                                                                                                                       <  2:2885505/150‑5 (MQ=255)
            aCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTg                                                                                                                                      <  1:4794401/150‑1 (MQ=255)
                 aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGtt                                                                                                                                 <  1:2708166/150‑1 (MQ=255)
                  tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGttt                                                                                                                                >  2:569653/1‑150 (MQ=255)
                   gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGTGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTttg                                                                                                                               >  1:1258450/1‑150 (MQ=255)
                     aCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTttgtt                                                                                                                             <  1:5189769/150‑1 (MQ=255)
                      cATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTttgttg                                                                                                                            <  1:1546255/150‑1 (MQ=255)
                       aTTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTtgttgt                                                                                                                           <  1:3531938/150‑1 (MQ=255)
                         ttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAAATGTTGCGGTTTGTtgtg                                                                                                                          >  1:331682/1‑150 (MQ=255)
                          tGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTa                                                                                                                        >  1:2022609/1‑150 (MQ=255)
                             aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACt                                                                                                                     >  1:621603/1‑150 (MQ=255)
                               gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTcc                                                                                                                   >  1:376599/1‑150 (MQ=255)
                               gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTcc                                                                                                                   <  1:558782/150‑1 (MQ=255)
                                 gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                                 <  1:1368891/150‑1 (MQ=255)
                                  aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATACTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGc                                                                                                                >  1:1780445/1‑150 (MQ=255)
                                     gcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGac                                                                                                             >  1:3116460/1‑150 (MQ=255)
                                      cagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacg                                                                                                            <  1:638732/150‑1 (MQ=255)
                                       agTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                           >  2:3796167/1‑150 (MQ=255)
                                             tCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCtt                                                                                                     >  1:965341/1‑150 (MQ=255)
                                             tCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCtt                                                                                                     <  1:658247/150‑1 (MQ=255)
                                              cTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTc                                                                                                    >  2:2135052/1‑150 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                >  1:4682763/1‑150 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                >  2:1688266/1‑150 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                                <  2:2651076/150‑1 (MQ=255)
                                                    tCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCa                                                                                              <  1:254948/150‑1 (MQ=255)
                                                      gCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAt                                                                                            <  2:2196818/150‑1 (MQ=255)
                                                       ccATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                           >  1:3837017/1‑150 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                      >  2:774591/1‑150 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                      <  2:818410/150‑1 (MQ=255)
                                                             aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                     >  1:1044206/1‑150 (MQ=255)
                                                               acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGttagttag                                                                                   <  1:2671175/150‑1 (MQ=255)
                                                                 accacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcg                                                                                 <  2:655285/150‑1 (MQ=255)
                                                                   cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                               <  2:5110875/150‑1 (MQ=255)
                                                                    acGAACTTATTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTGGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCca                                                                              <  2:4769888/150‑1 (MQ=255)
                                                                       aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                           <  2:2586863/150‑1 (MQ=255)
                                                                         cGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAg                                                                         <  2:4467754/150‑1 (MQ=255)
                                                                         cGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAg                                                                         >  1:2481430/1‑150 (MQ=255)
                                                                             tttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaaa                                                                     >  1:5238497/1‑150 (MQ=255)
                                                                               tGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACa                                                                   >  2:2725286/1‑150 (MQ=255)
                                                                               tGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACAGTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACa                                                                   >  2:35062/1‑150 (MQ=255)
                                                                                 cgcATGTCAAAGTTCATGCTCTATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTTGCGCCACAAAGGAAACAGc                                                                 <  1:10099/150‑1 (MQ=255)
                                                                                   cATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAt                                                               >  2:3619749/1‑150 (MQ=255)
                                                                                    aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                              >  1:5302110/1‑150 (MQ=255)
                                                                                     tGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                             >  2:4056903/1‑150 (MQ=255)
                                                                                       tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTccc                                                           <  2:2863991/150‑1 (MQ=255)
                                                                                       tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTccc                                                           <  1:4562844/150‑1 (MQ=255)
                                                                                        cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          >  1:2951459/1‑150 (MQ=255)
                                                                                        cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          >  1:2837413/1‑150 (MQ=255)
                                                                                        cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                          >  2:456265/1‑150 (MQ=255)
                                                                                         aaaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcgg                                                         <  1:2420593/150‑1 (MQ=255)
                                                                                           aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                       >  1:5028825/1‑150 (MQ=255)
                                                                                              tCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgag                                                    >  1:3979961/1‑150 (MQ=255)
                                                                                               cATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGAGgaga                                                   >  1:1085182/1‑150 (MQ=255)
                                                                                                aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTAAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                  >  1:4352221/1‑150 (MQ=255)
                                                                                                  gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAttt                                                >  2:3156740/1‑150 (MQ=255)
                                                                                                    tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAAAATGTTGCGGTGTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGACACAGCATTCCCGGCGGAGATTTg                                               >  2:19370/1‑150 (MQ=255)
                                                                                                    tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                              >  1:4905827/1‑150 (MQ=255)
                                                                                                      aaTGCGTGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                            <  2:1258450/150‑1 (MQ=255)
                                                                                                      aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                            <  1:4238607/150‑1 (MQ=255)
                                                                                                      aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                            <  1:4163481/150‑1 (MQ=255)
                                                                                                          cGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCcg                                        <  2:4201306/150‑1 (MQ=255)
                                                                                                                 aaCTGAGTATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACTCTTCATCTCAATGGTAGTTAGCGCCACAAAGGACACAGCATTCCCGGCGGAGATTTGTATGCCGCGCAAtt                                 >  1:4070987/1‑150 (MQ=255)
                                                                                                                  aCTGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                                >  2:2531212/1‑150 (MQ=255)
                                                                                                                    tGAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                              <  2:1314880/150‑1 (MQ=255)
                                                                                                                     gAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                             <  1:3200425/150‑1 (MQ=255)
                                                                                                                     gAGGATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                             <  1:5155790/150‑1 (MQ=255)
                                                                                                                       ggATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAg                           <  1:757550/150‑1 (MQ=255)
                                                                                                                        gATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGc                          <  1:2398412/150‑1 (MQ=255)
                                                                                                                        gATTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGc                          <  1:1709983/150‑1 (MQ=255)
                                                                                                                         aTTCCTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCa                         >  1:1208897/1‑150 (MQ=255)
                                                                                                                             cTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGAt                     <  2:686602/150‑1 (MQ=255)
                                                                                                                             cTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGAt                     >  2:398036/1‑150 (MQ=255)
                                                                                                                             cTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGAt                     <  1:4498656/150‑1 (MQ=255)
                                                                                                                             cTGCATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGAt                     >  1:1349045/1‑150 (MQ=255)
                                                                                                                                cATCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                  <  2:2621575/150‑1 (MQ=255)
                                                                                                                                 aTCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt                 >  1:3617731/1‑150 (MQ=255)
                                                                                                                                 aTCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt                 >  1:2102937/1‑150 (MQ=255)
                                                                                                                                 aTCACATTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt                 >  1:4646894/1‑150 (MQ=255)
                                                                                                                                   cacaTTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtat               <  2:3378767/150‑1 (MQ=255)
                                                                                                                                    acaTTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt              <  2:3256527/150‑1 (MQ=255)
                                                                                                                                    acaTTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt              >  2:368476/1‑150 (MQ=255)
                                                                                                                                      aTTGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat            <  1:3720803/150‑1 (MQ=255)
                                                                                                                                       ttGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc           <  2:5221522/150‑1 (MQ=255)
                                                                                                                                        tGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc          >  1:3349197/1‑150 (MQ=255)
                                                                                                                                        tGATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc          <  2:1877999/150‑1 (MQ=255)
                                                                                                                                         gATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa         <  2:5053654/150‑1 (MQ=255)
                                                                                                                                         gATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa         <  1:2135052/150‑1 (MQ=255)
                                                                                                                                         gATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa         <  1:3470292/150‑1 (MQ=255)
                                                                                                                                         gATATTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa         >  1:2201691/1‑150 (MQ=255)
                                                                                                                                          atatTTCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg        >  1:737457/1‑150 (MQ=255)
                                                                                                                                              ttCGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa    >  2:2044567/1‑150 (MQ=255)
                                                                                                                                                cGCATA‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAat  <  2:4099643/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑‑AAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAAT  >  CP009273/2108071‑2108363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A