Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A98.7% 744.0 / 4.3 227coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (103/121);  total (105/122)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 248 total aligned reads displayed.

TTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108073‑2108361
                                                                                                                                                   |                                                                                                                                               
    tcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaa                                                                                                                                           >  1:3386465/1‑148 (MQ=255)
    tcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaa                                                                                                                                           >  2:2247633/1‑148 (MQ=255)
       ttcaCCGCATGAACATTTGCAAGAGACTGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaa                                                                                                                                        <  2:1352574/148‑2 (MQ=255)
       ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaa                                                                                                                                        <  1:303686/148‑2 (MQ=255)
           ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaatgtt                                                                                                                                    <  1:2714289/148‑6 (MQ=255)
           ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaatgtt                                                                                                                                    <  2:476340/148‑6 (MQ=255)
              cATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCg                                                                                                                                  >  2:402745/1‑148 (MQ=255)
               aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCgg                                                                                                                                 >  1:2187491/1‑148 (MQ=255)
                 gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGtt                                                                                                                               >  1:1200975/1‑148 (MQ=255)
                 gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGtt                                                                                                                               <  2:1148642/148‑1 (MQ=255)
                 gAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGtt                                                                                                                               <  1:2027619/148‑1 (MQ=255)
                      tttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTttgttg                                                                                                                          <  2:2295022/148‑1 (MQ=255)
                          cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTa                                                                                                                      >  1:3182373/1‑148 (MQ=255)
                            agagACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACAGTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAAc                                                                                                                    >  2:3098708/1‑148 (MQ=255)
                             gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACt                                                                                                                   >  2:1068564/1‑148 (MQ=255)
                              agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTc                                                                                                                  >  1:502987/1‑148 (MQ=255)
                              agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTc                                                                                                                  >  1:502785/1‑148 (MQ=255)
                              agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTc                                                                                                                  >  2:2989670/1‑148 (MQ=255)
                               gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTcc                                                                                                                 >  1:2625284/1‑148 (MQ=255)
                                aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCa                                                                                                                >  1:1185732/1‑148 (MQ=255)
                                 cagcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                               >  1:1003860/1‑148 (MQ=255)
                                    cagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGa                                                                                                            >  2:910261/1‑148 (MQ=255)
                                       tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                         >  2:2752160/1‑148 (MQ=255)
                                       tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                         >  2:2937883/1‑148 (MQ=255)
                                          ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      <  1:2570843/148‑1 (MQ=255)
                                          ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  1:2093107/1‑148 (MQ=255)
                                              tttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTc                                                                                                  >  1:988897/1‑148 (MQ=255)
                                                    gCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCa                                                                                            <  2:2242/148‑1 (MQ=255)
                                                     ccATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCaa                                                                                           <  2:2019560/148‑1 (MQ=255)
                                                     ccATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCaa                                                                                           >  2:2058102/1‑148 (MQ=255)
                                                      cATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAt                                                                                          <  2:1608246/148‑1 (MQ=255)
                                                          aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtta                                                                                      >  2:1924886/1‑148 (MQ=255)
                                                            aaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCAAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                    <  1:373096/148‑1 (MQ=255)
                                                             acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   >  1:677792/1‑148 (MQ=255)
                                                             acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   <  2:2751524/148‑1 (MQ=255)
                                                             acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   <  2:922060/148‑1 (MQ=255)
                                                             acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   <  1:3309551/148‑1 (MQ=255)
                                                              caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  <  1:3110043/148‑1 (MQ=255)
                                                                 cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcgc                                                                              >  1:1540337/1‑148 (MQ=255)
                                                                  acGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcgc                                                                              <  1:1031987/148‑1 (MQ=255)
                                                                   cGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                             >  2:737213/1‑148 (MQ=255)
                                                                    gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCca                                                                            >  1:1031711/1‑148 (MQ=255)
                                                                    gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCca                                                                            <  1:1308939/148‑1 (MQ=255)
                                                                    gAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCca                                                                            >  1:2373305/1‑148 (MQ=255)
                                                                      aCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcaca                                                                          >  2:3399985/1‑148 (MQ=255)
                                                                             tGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaaa                                                                   <  2:3564662/148‑1 (MQ=255)
                                                                             tGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaaa                                                                   <  2:277979/148‑1 (MQ=255)
                                                                                     tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                           >  1:3462009/1‑148 (MQ=255)
                                                                                     tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                           <  2:632083/148‑1 (MQ=255)
                                                                                     tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                           <  1:1756406/148‑1 (MQ=255)
                                                                                     tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                           <  2:2074556/148‑1 (MQ=255)
                                                                                     tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                           <  1:493026/148‑1 (MQ=255)
                                                                                      cAAAGTTCATGCTCAATGCGTGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AATAAAAATGTTTCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGATACAGCATTcc                                                          <  1:389920/148‑1 (MQ=255)
                                                                                      cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          >  1:656071/1‑148 (MQ=255)
                                                                                      cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          <  2:496109/148‑1 (MQ=255)
                                                                                        aaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                        <  1:1597789/148‑1 (MQ=255)
                                                                                         aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcgg                                                       >  2:2733611/1‑148 (MQ=255)
                                                                                          gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggc                                                      >  1:1491454/1‑148 (MQ=255)
                                                                                               tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 <  2:1414980/148‑1 (MQ=255)
                                                                                               tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 <  1:1068564/148‑1 (MQ=255)
                                                                                                gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                <  1:1802012/148‑1 (MQ=255)
                                                                                                   cAATGCTGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             <  1:2435669/148‑1 (MQ=255)
                                                                                                   cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             <  1:1395475/148‑1 (MQ=255)
                                                                                                      tGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                          >  1:2610175/1‑148 (MQ=255)
                                                                                                      tGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAAt                                          >  2:429813/1‑148 (MQ=255)
                                                                                                       gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                         <  2:2823895/148‑1 (MQ=255)
                                                                                                               aaCTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCaa                                 <  1:737213/148‑1 (MQ=255)
                                                                                                                aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                >  2:2694784/1‑148 (MQ=255)
                                                                                                                aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                <  1:2989670/148‑1 (MQ=255)
                                                                                                                 cTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAtt                               >  2:78583/1‑148 (MQ=255)
                                                                                                                 cTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAtt                               <  1:405702/148‑1 (MQ=255)
                                                                                                                   gAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                             <  1:3412049/148‑1 (MQ=255)
                                                                                                                   gAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                             >  2:1035965/1‑148 (MQ=255)
                                                                                                                    aGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                            >  2:2311557/1‑148 (MQ=255)
                                                                                                                     ggATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                           >  1:253450/1‑148 (MQ=255)
                                                                                                                     ggATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                           >  1:1104466/1‑148 (MQ=255)
                                                                                                                     ggATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                           <  2:659264/148‑1 (MQ=255)
                                                                                                                     ggATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCACAATTtata                           >  1:3194462/1‑148 (MQ=255)
                                                                                                                      gATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATaa                          <  2:2463113/148‑1 (MQ=255)
                                                                                                                      gATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATaa                          >  1:2801482/1‑148 (MQ=255)
                                                                                                                           cTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                     <  2:2736709/148‑1 (MQ=255)
                                                                                                                           cTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                     <  1:1924886/148‑1 (MQ=255)
                                                                                                                           cTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAg                     <  1:23097/148‑1 (MQ=255)
                                                                                                                            tGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGa                    >  2:2383374/1‑148 (MQ=255)
                                                                                                                             gCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGAt                   <  2:2187486/148‑1 (MQ=255)
                                                                                                                              cATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  1:2868209/148‑1 (MQ=255)
                                                                                                                              cATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  1:615851/148‑1 (MQ=255)
                                                                                                                               aTCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAGGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGa                 >  2:1290714/1‑148 (MQ=255)
                                                                                                                               aTCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGa                 <  1:912127/148‑1 (MQ=255)
                                                                                                                                 cacaTTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               <  2:702255/148‑1 (MQ=255)
                                                                                                                                 cacaTTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               >  2:1852800/1‑148 (MQ=255)
                                                                                                                                    aTTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt            <  1:2752125/148‑1 (MQ=255)
                                                                                                                                      tGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          <  2:1003860/148‑1 (MQ=255)
                                                                                                                                      tGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          >  2:1788920/1‑148 (MQ=255)
                                                                                                                                      tGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          >  1:887530/1‑148 (MQ=255)
                                                                                                                                       gATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc         <  2:108252/148‑1 (MQ=255)
                                                                                                                                        atatTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGTGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        <  2:1537755/148‑1 (MQ=255)
                                                                                                                                        atatTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        <  2:3064297/148‑1 (MQ=255)
                                                                                                                                         tatTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa       >  2:2606158/1‑148 (MQ=255)
                                                                                                                                              cGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa  <  2:3367870/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108073‑2108361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A