Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A98.5% 902.5 / 6.3 263coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (0/4);  new base A (119/140);  total (119/144)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 285 total aligned reads displayed.

CCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108071‑2108361
                                                                                                                                                     |                                                                                                                                                
 cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑a                                                                                                                                                 >  2:2054595/1‑149 (MQ=255)
  tttttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa                                                                                                                                                >  2:4104012/1‑149 (MQ=255)
    tttCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaa‑a                                                                                                                                             >  2:2662464/1‑149 (MQ=255)
     ttcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaa‑aa                                                                                                                                            >  1:900929/1‑149 (MQ=255)
        attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaa‑aaaaa                                                                                                                                         <  2:2453956/149‑2 (MQ=255)
               gCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCg                                                                                                                                  >  1:4353547/1‑149 (MQ=255)
                  tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGtt                                                                                                                               <  2:4652282/149‑1 (MQ=255)
                  tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGtt                                                                                                                               <  2:4179824/149‑1 (MQ=255)
                    aaCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTttg                                                                                                                             <  1:565493/149‑1 (MQ=255)
                    aaCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTttg                                                                                                                             <  1:2166199/149‑1 (MQ=255)
                     aCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTttgt                                                                                                                            >  2:1528991/1‑149 (MQ=255)
                      cATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTttgtt                                                                                                                           >  1:3035063/1‑149 (MQ=255)
                      cATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTttgtt                                                                                                                           <  1:2081845/149‑1 (MQ=255)
                            cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTaa                                                                                                                     <  1:1286237/149‑1 (MQ=255)
                             aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAAc                                                                                                                    >  1:2775532/1‑149 (MQ=255)
                               gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTc                                                                                                                  <  2:1029755/149‑1 (MQ=255)
                               gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTc                                                                                                                  <  1:1674902/149‑1 (MQ=255)
                                 gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCa                                                                                                                >  2:2615314/1‑149 (MQ=255)
                                  aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTTTCTCCAg                                                                                                               >  1:3740810/1‑149 (MQ=255)
                                  aCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                               >  1:3741063/1‑149 (MQ=255)
                                   cagcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGc                                                                                                              <  2:4000733/149‑1 (MQ=255)
                                    agcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCg                                                                                                             <  1:4106913/149‑1 (MQ=255)
                                    agcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCg                                                                                                             <  2:3628183/149‑1 (MQ=255)
                                    agcagTGTGTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCg                                                                                                             <  2:4077686/149‑1 (MQ=255)
                                     gcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGa                                                                                                            >  1:1952473/1‑149 (MQ=255)
                                       agTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacg                                                                                                          >  1:1706555/1‑149 (MQ=255)
                                          gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacgcac                                                                                                       <  1:1867360/149‑1 (MQ=255)
                                           tttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  2:1203047/1‑149 (MQ=255)
                                           tttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  1:965047/1‑149 (MQ=255)
                                               ttttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTc                                                                                                  >  2:146990/1‑149 (MQ=255)
                                                tttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCa                                                                                                 >  2:255349/1‑149 (MQ=255)
                                                  tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtc                                                                                               <  1:17401/149‑1 (MQ=255)
                                                   ttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                              <  2:2565708/149‑1 (MQ=255)
                                                   ttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                              <  2:4354389/149‑1 (MQ=255)
                                                   ttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtct                                                                                              >  1:2625680/1‑149 (MQ=255)
                                                         aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgt                                                                                        >  1:4248541/1‑149 (MQ=255)
                                                          tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtt                                                                                       >  1:2931155/1‑149 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttag                                                                                     >  1:2965544/1‑149 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttag                                                                                     >  1:1222538/1‑149 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttag                                                                                     >  1:4750458/1‑149 (MQ=255)
                                                            aaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                    <  1:3011777/149‑1 (MQ=255)
                                                             aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                    >  2:2936030/1‑149 (MQ=255)
                                                             aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagt                                                                                    >  1:120583/1‑149 (MQ=255)
                                                              aaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   >  1:188489/1‑149 (MQ=255)
                                                               acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  <  1:631951/149‑1 (MQ=255)
                                                               acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  2:3480689/1‑149 (MQ=255)
                                                               acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  1:2901164/1‑149 (MQ=255)
                                                               acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  2:3695879/1‑149 (MQ=255)
                                                                caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGttagttag                                                                                 <  1:1297197/149‑1 (MQ=255)
                                                                 accacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                <  1:3547267/149‑1 (MQ=255)
                                                                 accacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                <  1:3396545/149‑1 (MQ=255)
                                                                 accacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTAAGTTAgc                                                                                >  1:1017094/1‑149 (MQ=255)
                                                                        aCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACaa                                                                         >  2:836552/1‑149 (MQ=255)
                                                                           tCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                      >  2:2804324/1‑149 (MQ=255)
                                                                           tCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                      <  1:721092/149‑1 (MQ=255)
                                                                             tttGCGCATGTCATAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAAATCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaa                                                                    <  1:469318/149‑1 (MQ=255)
                                                                             tttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaa                                                                    >  2:472544/1‑149 (MQ=255)
                                                                             tttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaa                                                                    <  2:2487947/149‑1 (MQ=255)
                                                                             tttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGAGTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaa                                                                    <  2:3795078/149‑1 (MQ=255)
                                                                              ttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGaaa                                                                   <  2:1814685/149‑1 (MQ=255)
                                                                               tGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAAc                                                                  <  1:653837/149‑1 (MQ=255)
                                                                                    aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAt                                                             <  1:1377389/149‑1 (MQ=255)
                                                                                     tGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                            >  2:2041858/1‑149 (MQ=255)
                                                                                       tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          >  2:1627313/1‑149 (MQ=255)
                                                                                         aaaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCCATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                        <  1:1013966/149‑1 (MQ=255)
                                                                                           aGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggc                                                      >  2:4022616/1‑149 (MQ=255)
                                                                                              tCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                   >  1:393883/1‑149 (MQ=255)
                                                                                              tCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                   >  1:617687/1‑149 (MQ=255)
                                                                                                aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 >  1:1469997/1‑149 (MQ=255)
                                                                                                  gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                               >  1:1168372/1‑149 (MQ=255)
                                                                                                  gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                               <  2:341406/149‑1 (MQ=255)
                                                                                                    tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             >  2:1980390/1‑149 (MQ=255)
                                                                                                    tCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             <  1:3320435/149‑1 (MQ=255)
                                                                                                         gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGc                                        <  2:2858488/149‑1 (MQ=255)
                                                                                                         gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGc                                        <  1:3457031/149‑1 (MQ=255)
                                                                                                                 aaCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                <  2:445138/149‑1 (MQ=255)
                                                                                                                 aaCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                <  1:3987748/149‑1 (MQ=255)
                                                                                                                   cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                              <  1:4264367/149‑1 (MQ=255)
                                                                                                                   cTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAACAAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                              <  2:3190875/149‑1 (MQ=255)
                                                                                                                    tGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCCATGta                             >  2:2221712/1‑149 (MQ=255)
                                                                                                                    tGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                             >  2:3152685/1‑149 (MQ=255)
                                                                                                                    tGAGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                             >  1:2541902/1‑149 (MQ=255)
                                                                                                                      aGGATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                           >  1:3184610/1‑149 (MQ=255)
                                                                                                                       ggATTCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATaa                          >  2:1107068/1‑149 (MQ=255)
                                                                                                                          ttCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCa                       >  2:1297128/1‑149 (MQ=255)
                                                                                                                          ttCCTGCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCa                       <  2:1047233/149‑1 (MQ=255)
                                                                                                                               gCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  1:479853/149‑1 (MQ=255)
                                                                                                                               gCATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  1:3852448/149‑1 (MQ=255)
                                                                                                                                cATCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGa                 <  2:1443111/149‑1 (MQ=255)
                                                                                                                                 aTCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                <  2:928698/149‑1 (MQ=255)
                                                                                                                                  tCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               >  1:2988735/1‑149 (MQ=255)
                                                                                                                                  tCACATTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               <  1:2332685/149‑1 (MQ=255)
                                                                                                                                    acaTTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtat             <  1:1846236/149‑1 (MQ=255)
                                                                                                                                      aTTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           <  1:3796320/149‑1 (MQ=255)
                                                                                                                                      aTTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           >  2:3265716/1‑149 (MQ=255)
                                                                                                                                      aTTGATATTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           >  2:3037636/1‑149 (MQ=255)
                                                                                                                                          atatTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa       <  1:4432908/149‑1 (MQ=255)
                                                                                                                                           tatTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg      <  1:4060091/149‑1 (MQ=255)
                                                                                                                                            atTTCGCATAAAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGt     >  2:4244057/1‑149 (MQ=255)
                                                                                                                                               tCGCAT‑AAA‑AAAATTGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa  <  2:3877376/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAA‑AAAA‑TGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108071‑2108361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A