Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A99.6% 796.4 / ‑3.6 240coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (130/109);  total (130/110)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 256 total aligned reads displayed.

CTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108072‑2108361
                                                                                                                                                    |                                                                                                                                               
cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAt                                                                                                                                                  >  2:944993/1‑148 (MQ=255)
       attcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaa‑aaa                                                                                                                                         >  1:1541901/1‑148 (MQ=255)
        ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaa‑aaaa                                                                                                                                        >  1:1677818/1‑147 (MQ=255)
         tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaa‑aaaat                                                                                                                                       <  1:1420954/148‑3 (MQ=255)
           aCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aaaa‑aaaatgt                                                                                                                                     <  1:3541639/148‑5 (MQ=255)
              gCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGc                                                                                                                                   >  2:1744107/1‑148 (MQ=255)
               cATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCg                                                                                                                                  >  2:1520248/1‑148 (MQ=255)
                 tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGt                                                                                                                                >  1:1110162/1‑148 (MQ=255)
                 tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGt                                                                                                                                <  1:3174181/148‑1 (MQ=255)
                   aaCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGttt                                                                                                                              >  2:3162122/1‑148 (MQ=255)
                      aTTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGGGGTttgtt                                                                                                                           >  2:792044/1‑148 (MQ=255)
                       tttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTttgttg                                                                                                                          >  2:2918751/1‑148 (MQ=255)
                       tttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTgtgttg                                                                                                                          >  2:2103629/1‑148 (MQ=255)
                         tGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTtgtg                                                                                                                        <  1:1944572/148‑1 (MQ=255)
                           cAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTa                                                                                                                      >  1:572393/1‑148 (MQ=255)
                             agagACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTGTGTTGTGTAAc                                                                                                                    >  2:1321377/1‑148 (MQ=255)
                              gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACt                                                                                                                   <  2:1735097/148‑1 (MQ=255)
                              gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACt                                                                                                                   >  2:1569126/1‑148 (MQ=255)
                                gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTcc                                                                                                                 <  1:2745255/148‑1 (MQ=255)
                                  cagcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                               >  1:2219181/1‑148 (MQ=255)
                                   agcagTGTTTATTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGc                                                                                                              >  2:2500284/1‑148 (MQ=255)
                                     cagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGa                                                                                                            >  2:1598504/1‑148 (MQ=255)
                                       gtgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGTATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGacg                                                                                                          <  1:1460826/148‑1 (MQ=255)
                                        tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                         >  2:2236493/1‑148 (MQ=255)
                                         gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgca                                                                                                        <  2:2357613/148‑1 (MQ=255)
                                         gtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTAATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgca                                                                                                        <  1:3271323/148‑1 (MQ=255)
                                           ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  2:2927857/1‑148 (MQ=255)
                                           ttCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacg                                                                                                      >  2:2026731/1‑148 (MQ=255)
                                             cTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCt                                                                                                    >  1:1738286/1‑148 (MQ=255)
                                             cTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCt                                                                                                    >  1:1023442/1‑148 (MQ=255)
                                              ttttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCtt                                                                                                   >  2:2081453/1‑148 (MQ=255)
                                                ttttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCa                                                                                                 <  2:125277/148‑1 (MQ=255)
                                                     gCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCa                                                                                            <  2:3037557/148‑1 (MQ=255)
                                                     gCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCa                                                                                            <  2:1158442/148‑1 (MQ=255)
                                                      ccATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCaa                                                                                           >  2:488100/1‑148 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         >  2:1380031/1‑148 (MQ=255)
                                                          aaaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtt                                                                                       >  2:569404/1‑148 (MQ=255)
                                                            aaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCAAGCGACGCACGCTTCATCTCAATgttag                                                                                     >  1:1893905/1‑148 (MQ=255)
                                                              acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   >  1:1085524/1‑148 (MQ=255)
                                                               caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  2:1866891/1‑148 (MQ=255)
                                                                accacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGttagttag                                                                                 >  2:1257431/1‑148 (MQ=255)
                                                                 ccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgc                                                                                >  2:244528/1‑148 (MQ=255)
                                                                  cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcg                                                                               <  1:2275911/148‑1 (MQ=255)
                                                                   acGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcgc                                                                              <  2:26874/148‑1 (MQ=255)
                                                                    cGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                             >  2:2543733/1‑148 (MQ=255)
                                                                          tCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAg                                                                       >  1:2625182/1‑148 (MQ=255)
                                                                          tCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAg                                                                       <  1:1278707/148‑1 (MQ=255)
                                                                           cTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                      >  1:847987/1‑148 (MQ=255)
                                                                           cTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                      >  1:928367/1‑148 (MQ=255)
                                                                            tttGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGa                                                                     <  2:1504798/148‑1 (MQ=255)
                                                                                   aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCa                                                              <  1:522966/148‑1 (MQ=255)
                                                                                   aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCa                                                              <  1:1741796/148‑1 (MQ=255)
                                                                                       cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          <  2:2571728/148‑1 (MQ=255)
                                                                                       cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          >  2:2532745/1‑148 (MQ=255)
                                                                                         aaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcg                                                        <  1:3406343/148‑1 (MQ=255)
                                                                                           gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggc                                                      >  1:3002899/1‑148 (MQ=255)
                                                                                           gTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggc                                                      >  1:2815957/1‑148 (MQ=255)
                                                                                            ttCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                     <  2:3545090/148‑1 (MQ=255)
                                                                                             tCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcgg                                                    <  1:2349391/148‑1 (MQ=255)
                                                                                               aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgag                                                  <  2:333998/148‑1 (MQ=255)
                                                                                                tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 <  1:2871865/148‑1 (MQ=255)
                                                                                                tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 <  2:710918/148‑1 (MQ=255)
                                                                                                 gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                >  2:2216114/1‑148 (MQ=255)
                                                                                                  cTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                               >  2:3512508/1‑148 (MQ=255)
                                                                                                    cAATGCGGGTCCATCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             <  2:469898/148‑1 (MQ=255)
                                                                                                     aaTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAACAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                            <  2:1684844/148‑1 (MQ=255)
                                                                                                        gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                         >  2:3455175/1‑148 (MQ=255)
                                                                                                               cAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCa                                  <  1:996803/148‑1 (MQ=255)
                                                                                                                aaCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCaa                                 <  2:2300169/148‑1 (MQ=255)
                                                                                                                aaCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCaa                                 <  1:488100/148‑1 (MQ=255)
                                                                                                                 aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                <  2:1999617/148‑1 (MQ=255)
                                                                                                                 aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                <  1:1753752/148‑1 (MQ=255)
                                                                                                                 aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAt                                >  1:1215073/1‑148 (MQ=255)
                                                                                                                  cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAtt                               >  2:1431324/1‑148 (MQ=255)
                                                                                                                  cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAtt                               >  2:2593216/1‑148 (MQ=255)
                                                                                                                  cTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAtt                               >  2:808979/1‑148 (MQ=255)
                                                                                                                   tGAGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAttt                              <  2:231557/148‑1 (MQ=255)
                                                                                                                     aGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                            >  1:2671340/1‑148 (MQ=255)
                                                                                                                     aGGATTCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                            <  2:3479957/148‑1 (MQ=255)
                                                                                                                          tCCTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCa                       >  1:3475970/1‑148 (MQ=255)
                                                                                                                            cTGCATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGAAATTTATAAGCAAg                     <  1:3494380/148‑1 (MQ=255)
                                                                                                                               cATCACATTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  2:906632/148‑1 (MQ=255)
                                                                                                                                  cacaTTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               >  2:273288/1‑148 (MQ=255)
                                                                                                                                  cacaTTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               <  2:413612/148‑1 (MQ=255)
                                                                                                                                  cacaTTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGt               >  2:3460857/1‑148 (MQ=255)
                                                                                                                                   acaTTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGta              <  1:2917199/148‑1 (MQ=255)
                                                                                                                                     aTTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTACCTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGAGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt            <  2:572441/148‑1 (MQ=255)
                                                                                                                                     aTTGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatt            >  1:205350/1‑148 (MQ=255)
                                                                                                                                      ttGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           >  1:638283/1‑148 (MQ=255)
                                                                                                                                       tGATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtattat          <  2:3067488/148‑1 (MQ=255)
                                                                                                                                        gATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc         <  1:2022525/148‑1 (MQ=255)
                                                                                                                                        gATATTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATc         <  2:1774450/148‑1 (MQ=255)
                                                                                                                                         atatTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        <  2:3013898/148‑1 (MQ=255)
                                                                                                                                         atatTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        >  2:1050371/1‑148 (MQ=255)
                                                                                                                                          tatTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCa       >  1:3016178/1‑148 (MQ=255)
                                                                                                                                           atTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGTAATTTATAAGCAAGATGAGTATTATCCAg      >  1:1951033/1‑148 (MQ=255)
                                                                                                                                           atTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg      >  1:432141/1‑148 (MQ=255)
                                                                                                                                           atTTCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg      <  1:3191863/148‑1 (MQ=255)
                                                                                                                                             ttCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCCATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGtt    >  2:2879897/1‑148 (MQ=255)
                                                                                                                                              tCGCATAAAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTg   >  2:3000366/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAAA‑AAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108072‑2108361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A