Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,108,2191.A99.0% 638.6 / 0.9 193coding (1159/1395 nt)wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (87/104);  total (89/104)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 208 total aligned reads displayed.

CTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108072‑2108361
                                                                                                                                                    |                                                                                                                                               
cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAt                                                                                                                                                  >  1:788918/1‑148 (MQ=255)
cTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGAGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAt                                                                                                                                                  >  2:1211264/1‑148 (MQ=255)
     tcattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaa                                                                                                                                           >  1:2652378/1‑148 (MQ=255)
        ttcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaa                                                                                                                                        >  2:724136/1‑147 (MQ=255)
         tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaat                                                                                                                                       <  2:1386119/148‑3 (MQ=255)
         tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaat                                                                                                                                       <  2:3338985/148‑3 (MQ=255)
         tcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaat                                                                                                                                       <  2:60381/148‑3 (MQ=255)
          caCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaatg                                                                                                                                      <  2:554965/148‑4 (MQ=255)
            ccGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑aa‑aaaaaatgtt                                                                                                                                    >  1:698089/1‑143 (MQ=255)
                aTGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCgg                                                                                                                                 >  2:1176516/1‑148 (MQ=255)
                 tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGt                                                                                                                                <  1:1870312/148‑1 (MQ=255)
                 tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGt                                                                                                                                >  1:2016089/1‑148 (MQ=255)
                 tGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGt                                                                                                                                <  1:500562/148‑1 (MQ=255)
                    aCATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTttg                                                                                                                             <  2:832640/148‑1 (MQ=255)
                        ttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTtgttgt                                                                                                                         >  1:1818762/1‑148 (MQ=255)
                        ttGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTtgttgt                                                                                                                         <  2:8077/148‑1 (MQ=255)
                         tGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTtgtg                                                                                                                        <  2:2373917/148‑1 (MQ=255)
                         tGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTtgtg                                                                                                                        <  1:936448/148‑1 (MQ=255)
                            aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTaa                                                                                                                     <  2:672957/148‑1 (MQ=255)
                            aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTaa                                                                                                                     <  2:1892497/148‑1 (MQ=255)
                            aaGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTaa                                                                                                                     <  2:772917/148‑1 (MQ=255)
                            aaGAGACAGCAGTGTTTCATTTTTCGCCATAAAAACACCACGAACGTCTTTCCGCATGTCAAACTTCATGCTCAATGCGGGTCCAACAGAGGATTCCTGCATCAAATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTaa                                                                                                                     >  2:900068/1‑148 (MQ=255)
                              gagaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTACCt                                                                                                                   <  1:284436/148‑1 (MQ=255)
                               agaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTc                                                                                                                  <  2:2683421/148‑1 (MQ=255)
                                gaCAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTcc                                                                                                                 <  2:1027212/148‑1 (MQ=255)
                                  cagcagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAg                                                                                                               <  1:1452488/148‑1 (MQ=255)
                                        tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                         >  2:1124576/1‑148 (MQ=255)
                                        tgtTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgc                                                                                                         >  2:2416078/1‑148 (MQ=255)
                                            tCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGacgcacgc                                                                                                     <  2:3442997/148‑1 (MQ=255)
                                             cTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCt                                                                                                    >  1:671356/1‑148 (MQ=255)
                                                 tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAt                                                                                                >  1:2250313/1‑148 (MQ=255)
                                                 tttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAt                                                                                                <  1:1505806/148‑1 (MQ=255)
                                                  ttCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCAtc                                                                                               <  1:3119272/148‑1 (MQ=255)
                                                       cATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAt                                                                                          <  1:746164/148‑1 (MQ=255)
                                                       cATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAt                                                                                          >  1:3478046/1‑148 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         <  2:317166/148‑1 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         <  2:1628925/148‑1 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         >  1:1257677/1‑148 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         <  1:2915930/148‑1 (MQ=255)
                                                        aTAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AAGAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATg                                                                                         >  1:508996/1‑148 (MQ=255)
                                                         tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgt                                                                                        <  2:2494296/148‑1 (MQ=255)
                                                         tAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgt                                                                                        <  2:3253497/148‑1 (MQ=255)
                                                          aaaaaCACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgtt                                                                                       <  1:3035945/148‑1 (MQ=255)
                                                              acacCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtt                                                                                   <  1:3414335/148‑1 (MQ=255)
                                                               caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  1:3173975/1‑148 (MQ=255)
                                                               caccacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATgttagtta                                                                                  >  1:1629185/1‑148 (MQ=255)
                                                                  cacGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAgcg                                                                               <  2:1839023/148‑1 (MQ=255)
                                                                      aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcac                                                                           >  2:2089067/1‑148 (MQ=255)
                                                                       aCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCcaca                                                                          <  2:3308234/148‑1 (MQ=255)
                                                                           cTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                      >  2:496802/1‑148 (MQ=255)
                                                                           cTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAgg                                                                      >  1:1053037/1‑148 (MQ=255)
                                                                                  cATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGc                                                               >  2:3467844/1‑148 (MQ=255)
                                                                                  cATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGc                                                               <  2:3059256/148‑1 (MQ=255)
                                                                                  cATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGc                                                               <  2:728252/148‑1 (MQ=255)
                                                                                  cATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGc                                                               <  1:3313094/148‑1 (MQ=255)
                                                                                   aTGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCa                                                              >  1:2519555/1‑148 (MQ=255)
                                                                                     gTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCAtt                                                            >  1:461785/1‑148 (MQ=255)
                                                                                      tCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTc                                                           <  1:841865/148‑1 (MQ=255)
                                                                                       cAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTcc                                                          >  2:2773997/1‑148 (MQ=255)
                                                                                            ttCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcg                                                     >  2:1948355/1‑148 (MQ=255)
                                                                                             tCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCcggcgg                                                    >  2:1991015/1‑148 (MQ=255)
                                                                                              cATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCTACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGga                                                   >  2:3444459/1‑148 (MQ=255)
                                                                                               aTGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCTCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgag                                                  <  1:179720/148‑1 (MQ=255)
                                                                                                tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 >  1:1396667/1‑148 (MQ=255)
                                                                                                tGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGgaga                                                 <  2:1159263/148‑1 (MQ=255)
                                                                                                 gCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAt                                                >  2:352379/1‑148 (MQ=255)
                                                                                                  cTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGAtt                                               <  2:1818762/148‑1 (MQ=255)
                                                                                                    cAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                             >  2:3229909/1‑148 (MQ=255)
                                                                                                      aTGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGaa                                           >  1:1291187/1‑148 (MQ=255)
                                                                                                        gCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATg                                         <  1:1532424/148‑1 (MQ=255)
                                                                                                         cGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGc                                        <  1:2151233/148‑1 (MQ=255)
                                                                                                              ccAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCgcgc                                   >  1:470053/1‑148 (MQ=255)
                                                                                                                aaCTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCaa                                 >  2:2133315/1‑148 (MQ=255)
                                                                                                                  cTGAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAAtt                               >  1:2427239/1‑148 (MQ=255)
                                                                                                                    gAGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTta                             <  1:317593/148‑1 (MQ=255)
                                                                                                                     aGGATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                            >  2:3027934/1‑148 (MQ=255)
                                                                                                                     aGGAGTCCTGCATCACCTTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAAGGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtat                            <  1:1914698/148‑1 (MQ=255)
                                                                                                                      ggATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTtata                           <  1:1176516/148‑1 (MQ=255)
                                                                                                                       gATTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATaa                          <  2:3293818/148‑1 (MQ=255)
                                                                                                                        aTTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAg                         <  2:1799479/148‑1 (MQ=255)
                                                                                                                        aTTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAg                         <  1:3211595/148‑1 (MQ=255)
                                                                                                                        aTTCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAg                         <  1:3211487/148‑1 (MQ=255)
                                                                                                                         ttCCTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGc                        >  1:3297823/1‑148 (MQ=255)
                                                                                                                           ccTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCaa                      <  2:1256024/148‑1 (MQ=255)
                                                                                                                           ccTGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCaa                      <  1:1255814/148‑1 (MQ=255)
                                                                                                                             tGCATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGa                    <  1:3206139/148‑1 (MQ=255)
                                                                                                                               cATCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  2:1365796/148‑1 (MQ=255)
                                                                                                                               cATCACAGTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATg                  <  2:2908369/148‑1 (MQ=255)
                                                                                                                                aTCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGa                 >  2:2977390/1‑148 (MQ=255)
                                                                                                                                 tCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                <  1:1002788/148‑1 (MQ=255)
                                                                                                                                 tCACATTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAg                <  1:2757316/148‑1 (MQ=255)
                                                                                                                                    caTTGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtat             <  1:1948360/148‑1 (MQ=255)
                                                                                                                                      ttGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           <  1:550197/148‑1 (MQ=255)
                                                                                                                                      ttGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           <  1:2315313/148‑1 (MQ=255)
                                                                                                                                      ttGATATTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGtatta           >  2:2583205/1‑148 (MQ=255)
                                                                                                                                         atatTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        >  2:1188358/1‑148 (MQ=255)
                                                                                                                                         atatTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATcc        >  2:2938555/1‑148 (MQ=255)
                                                                                                                                           atTTCGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAg      >  1:1760779/1‑148 (MQ=255)
                                                                                                                                               cGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa  <  1:2416078/148‑1 (MQ=255)
                                                                                                                                               cGCATAAA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGa  >  1:1152505/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCAT‑AA‑AAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGA  >  CP009273/2108072‑2108361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A