Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,004,533 G→T 89.0% T151T (ACG→ACT)  yedL → putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,004,5330GT89.0% 348.6 / 27.3 165T151T (ACG→ACT) yedLputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (15/5);  new base T (58/81);  total (79/86)
Fisher's exact test for biased strand distribution p-value = 7.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT  >  CP009273/2004388‑2004676
                                                                                                                                                 |                                                                                                                                               
gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                               <  2:794856/148‑1 (MQ=255)
 aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                              <  2:2199374/148‑1 (MQ=255)
 aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                              <  2:3158677/148‑1 (MQ=255)
  tGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAt                                                                                                                                             <  2:177204/148‑1 (MQ=255)
   gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGTCTCTATg                                                                                                                                            >  2:128546/1‑148 (MQ=255)
    ccTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGc                                                                                                                                           >  2:125344/1‑148 (MQ=255)
    ccTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACGCTAttc                                                                                                                                           >  1:2678056/1‑146 (MQ=255)
       tCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATGACTCTATGCGaa                                                                                                                                        >  2:786644/1‑148 (MQ=255)
       tCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCATTGACACTATTCGaa                                                                                                                                        >  2:307062/1‑148 (MQ=255)
         aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaaaa                                                                                                                                      >  2:2317143/1‑148 (MQ=255)
         aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaaaa                                                                                                                                      <  2:3276157/148‑1 (MQ=255)
           cGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaata                                                                                                                                    <  1:1305392/148‑1 (MQ=255)
           cGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAaata                                                                                                                                    <  1:710771/148‑1 (MQ=255)
               ttaGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGa                                                                                                                                <  2:1808361/146‑1 (MQ=255)
               tGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGa                                                                                                                                <  2:2172448/148‑1 (MQ=255)
               tGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGa                                                                                                                                <  1:2785641/148‑1 (MQ=255)
                gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGaa                                                                                                                               <  2:1889279/148‑1 (MQ=255)
                gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGaa                                                                                                                               <  2:164435/148‑1 (MQ=255)
                gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGaa                                                                                                                               <  2:164177/148‑1 (MQ=255)
                 aGNTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTNATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAt                                                                                                                              <  2:1133612/148‑1 (MQ=255)
                 aGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAt                                                                                                                              <  2:1943326/148‑1 (MQ=255)
                 aGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAt                                                                                                                              <  2:2903533/148‑1 (MQ=255)
                  gCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGAATCTAAGCGAAAATaattaatt                                                                                                                             >  1:1361805/1‑148 (MQ=255)
                    ttAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGc                                                                                                                           <  2:2418734/148‑1 (MQ=255)
                    ttAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGc                                                                                                                           <  2:2096304/148‑1 (MQ=255)
                     tAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAAAGAATTTcc                                                                                                                          >  2:2102487/1‑148 (MQ=255)
                     tAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTTTAATTTTATATATTCGAAAAAaattaatttac                                                                                                                          >  1:2879429/1‑145 (MQ=255)
                     tAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCATTGTCTCTATGCGAAAATAATGAATTTcc                                                                                                                          >  2:2810150/1‑148 (MQ=255)
                       gAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAATTTTCTCTATGCGAAAATAATGAAtttgcat                                                                                                                        >  1:2130525/1‑143 (MQ=255)
                        aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTTTTTGAAGCATGGTGTCGCTGATTGCGTACCGTTTGCTGATTATCGACTGGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATg                                                                                                                       <  2:1002855/148‑1 (MQ=255)
                        aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATg                                                                                                                       <  1:2721223/148‑1 (MQ=255)
                        aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATg                                                                                                                       <  2:1002341/148‑1 (MQ=255)
                         aaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTCTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGa                                                                                                                      <  1:2226559/148‑1 (MQ=255)
                           cGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGc                                                                                                                    <  1:1955132/148‑1 (MQ=255)
                              gTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTTCTCTATTCGAAAATAATTAATTTACATGAGCCAg                                                                                                                 >  1:1714482/1‑148 (MQ=255)
                              gTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACACTATTCGAAAATAATGAATTTCCCTTAGCCaa                                                                                                                 >  2:2911100/1‑147 (MQ=255)
                              gTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAAAAATGAATTTCCATGAGCCAg                                                                                                                 >  2:1464778/1‑148 (MQ=255)
                               tACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGa                                                                                                                <  2:809425/148‑1 (MQ=255)
                                   caGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTCATTGACTCTATGCGAAAATAATGAATTGACAGGAGCCAGACgca                                                                                                            >  2:325442/1‑148 (MQ=255)
                                     ggCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACgcagc                                                                                                          <  1:829756/148‑1 (MQ=255)
                                     ggCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGAAGCTATGCGAAAATAAATAATTGCCAAGAGCCACAagcagc                                                                                                          >  1:2653446/1‑148 (MQ=255)
                                         ggATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAAACGCAGCACAt                                                                                                      >  2:767767/1‑148 (MQ=255)
                                         ggATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACACGAAGAGAAAATAAATAATTGCCAATAGCCAGACgcagcaaaa                                                                                                      >  2:1423380/1‑145 (MQ=255)
                                             ttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTTAGATATGCGAAAATAATGAATTGGCATGAGCCAGAAGCACcacaataat                                                                                                  >  1:2126697/1‑143 (MQ=255)
                                             ttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATGACTCGAAGCGAAAATAAATAAATGGCAAGAGCCACACGCACCACAATCtt                                                                                                  >  1:556929/1‑148 (MQ=255)
                                             ttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCAATGACTCTATGCGAAAATAATGAATTGCCATGAGCCCGACGCACCACATTcat                                                                                                  >  1:2218990/1‑146 (MQ=255)
                                                 ggCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCAt                                                                                              <  2:741177/148‑1 (MQ=255)
                                                  gCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCAtt                                                                                             <  2:1446920/148‑1 (MQ=255)
                                                     tGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTCTGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGa                                                                                          <  2:2330765/148‑1 (MQ=255)
                                                     tGCCAACAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGa                                                                                          <  2:2213241/148‑1 (MQ=255)
                                                            atcTTTATTTGAAGCATGGTTTCGCTGATTGCGAAACGTTTGCTGATTTTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGTATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgctg                                                                                   <  2:170488/146‑1 (MQ=255)
                                                              cTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTGAATCTATTCGAAAATAATTAATTTCGATGAGCCACACGCAGCACAATCATTGATTAGACGTGGTgcg                                                                                 >  1:1610563/1‑148 (MQ=255)
                                                              cTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATTCGAAAATAATGAATTTCCATGAGGCAGACGCAGCACATTATTGCATTAGACGTGCTgcg                                                                                 >  2:484697/1‑148 (MQ=255)
                                                              cTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGAAAATAATGAATTTCCATGAGCCACACGCACCACAATCTTGCAATCGACGTGCTgcg                                                                                 >  2:484654/1‑148 (MQ=255)
                                                              cTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTGTATTCGAAAATAATTTATTGCCATGAGCCAGACGCAGCACAATCTTGCATTCGACGTGCTgcg                                                                                 >  2:1551266/1‑148 (MQ=255)
                                                                ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATTAATCTATTCGAAAATAATGAATTTGCAGGTTCCACACGCAGCCCAATCTTGGCTTAGACGTGGTGCGcc                                                                               >  1:2529696/1‑148 (MQ=255)
                                                                ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATGACTCTATTCGAAAATAATGAATTGCCATGAGCCAGAAGCAGCACAATCTTGCAATCGACGTGCAGCGcc                                                                               >  2:55776/1‑148 (MQ=255)
                                                                 tATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCt                                                                              <  1:1745432/148‑1 (MQ=255)
                                                                     tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttat                                                                          <  2:195922/148‑1 (MQ=255)
                                                                     tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttat                                                                          >  1:2994695/1‑148 (MQ=255)
                                                                         gCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTttatttat                                                                      <  2:1383040/148‑1 (MQ=255)
                                                                           aTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCa                                                                    <  2:1386495/148‑1 (MQ=255)
                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAAATTTATCTATTCGAAAATAATTAAATTGCAAGATCCACACGCACCACCATATTTCATTCGAACTGCGGCGCCCTTATTTATAAc                                                                   >  1:1027540/1‑148 (MQ=255)
                                                                               tttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATGAATGACTCTAAGCGAAAATAATGAATTGCCATGAGCCACACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcag                                                                >  2:2663116/1‑148 (MQ=255)
                                                                                ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcagc                                                               <  1:248913/148‑1 (MQ=255)
                                                                                        ttGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACACTATGCGAAAATAATTAATTTCCATGAGCCAGACGCAGCACAATCTTGCATTCGACGTGCTGCGCCATTATTTATCACCAGCAGGAAACg                                                       >  2:2762746/1‑148 (MQ=255)
                                                                                        ttGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATGACTCTATGCGAAAATAATGAATTGCCAAGAGCCAGACGCAGCACAATCTTGCATTAGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACg                                                       >  2:225519/1‑148 (MQ=255)
                                                                                          gCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGcc                                                     <  1:2282473/148‑1 (MQ=255)
                                                                                           cGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGTGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCt                                                    <  1:811503/148‑1 (MQ=255)
                                                                                                cGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTcc                                               <  2:892637/148‑1 (MQ=255)
                                                                                                    tGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAg                                           <  1:3022717/148‑1 (MQ=255)
                                                                                                     gCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGa                                          <  1:125344/148‑1 (MQ=255)
                                                                                                           tataGACTTGTTACACACAGTCGATTTTTATAATTGACTCTTTTCGAAAATAATGAATTGCCATGAGCCAGACGAAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATGACCAGCTGGAAACGCCTTGTCCATAGACGcccg                                    <  2:316414/148‑2 (MQ=255)
                                                                                                              cGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGAAAATAATTTATTGCCATGAGCCAGACCCCGCACATTCTTTCATTCGACGTGCGGCGCCGTTATTTATCACCACCAGGAAACGCCGTGTCCATAGACGCCCCATc                                 >  2:468869/1‑148 (MQ=255)
                                                                                                                aCTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCca                               <  2:350103/148‑1 (MQ=255)
                                                                                                                 cTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcac                              <  1:1044951/148‑1 (MQ=255)
                                                                                                                 cTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcac                              <  1:1162568/148‑1 (MQ=255)
                                                                                                                 cTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcac                              <  1:2106108/148‑1 (MQ=255)
                                                                                                                  ttGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcaca                             <  1:2572818/148‑1 (MQ=255)
                                                                                                                  ttGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcaca                             <  2:2048471/148‑1 (MQ=255)
                                                                                                                   tGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTTCTTCGCCTTTATTTATAACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAt                            >  2:3012064/1‑148 (MQ=255)
                                                                                                                     aTCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACAATCTTGCATTCGACGTGCTGCGCCTTTATTTATAACCAGCAGGAAAAGCCTTGTCCATAGACGCCCAATCCACAAgc                          >  2:1850959/1‑148 (MQ=255)
                                                                                                                      tCCACACAGTCGATTTTTATCATTGAATCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATgcg                         >  2:765302/1‑148 (MQ=255)
                                                                                                                         acacaGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCca                      <  1:3131153/148‑1 (MQ=255)
                                                                                                                          cacaGTCGATTTTTATCAATGACTCTATGCGAAAATAATGAATTTCCAAGAGCCAGACGCAGCACAATATTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCGTGTCCATAGAAGCCCCATACACATGCGCcac                     >  2:3003684/1‑148 (MQ=255)
                                                                                                                              gTCGATTTTTATCATTTACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGa                 >  2:2971974/1‑148 (MQ=255)
                                                                                                                               tCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaa                >  1:832169/1‑148 (MQ=255)
                                                                                                                                cGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaaa               >  1:1923617/1‑148 (MQ=255)
                                                                                                                                  aTTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAcc             <  2:1298974/148‑1 (MQ=255)
                                                                                                                                   tttttATCATGGCCTCTATGCGAAAATAATGAATTGACATGAGCCAGACGCAGCAAATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGACTTGTCCATAGACGCCCCTTCCACATGCGCCACACGAAACct            <  1:2190060/148‑1 (MQ=255)
                                                                                                                                    ttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctc           <  2:937372/148‑1 (MQ=255)
                                                                                                                                     tttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctct          <  2:1744294/148‑1 (MQ=255)
                                                                                                                                       tATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAt        >  2:664632/1‑148 (MQ=255)
                                                                                                                                        aTCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAtt       >  2:183777/1‑148 (MQ=255)
                                                                                                                                          cATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc     <  1:900637/148‑1 (MQ=255)
                                                                                                                                          cATTGACTATATGCGAAAATAATGAATTGACATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCATTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc     <  1:2554381/148‑1 (MQ=255)
                                                                                                                                            ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg   <  1:2923401/148‑1 (MQ=255)
                                                                                                                                            ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg   <  1:2923571/148‑1 (MQ=255)
                                                                                                                                             tGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGt  >  2:584204/1‑148 (MQ=255)
                                                                                                                                             tGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGt  >  1:746024/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT  >  CP009273/2004388‑2004676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACA  >  CP009273/2004384‑2004682
                                                                                                                                                     |                                                                                                                                                     
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                       <  SRR19973070.2577892/150‑1 (MQ=60)
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                       <  SRR19973070.520998/150‑1 (MQ=60)
   AGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTA                                                                                                                                                    <  SRR19973070.705357/150‑1 (MQ=60)
      TGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACGCTATTC                                                                                                                                                 >  SRR19973070.2695442/1‑150 (MQ=60)
        CCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAAATGACTCTATGCGA                                                                                                                                               >  SRR19973070.2381392/1‑150 (MQ=60)
               CGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAAT                                                                                                                                        <  SRR19973070.1314463/150‑1 (MQ=60)
               CGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAAT                                                                                                                                        <  SRR19973070.715838/150‑1 (MQ=60)
                   TGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAT                                                                                                                                    <  SRR19973070.2803566/150‑1 (MQ=60)
                    GAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATT                                                                                                                                   <  SRR19973070.2122797/150‑1 (MQ=60)
                    GAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGAATCTAAGCGAAAATAATTAATT                                                                                                                                   >  SRR19973070.1371092/1‑150 (MQ=60)
                     AGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTG                                                                                                                                  <  SRR19973070.2701026/150‑1 (MQ=60)
                       CTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCC                                                                                                                                <  SRR19973070.863505/150‑1 (MQ=60)
                         TAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAATTTTCTCTATGCGAAAATAATGAATTtgcat                                                                                                                              >  SRR19973070.2144772/1‑145 (MQ=60)
                            AAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAG                                                                                                                           <  SRR19973070.939339/150‑1 (MQ=60)
                            AAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAG                                                                                                                           <  SRR19973070.2738786/150‑1 (MQ=60)
                             AACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTCTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGC                                                                                                                          <  SRR19973070.2241199/150‑1 (MQ=60)
                               CGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCA                                                                                                                        <  SRR19973070.1968294/150‑1 (MQ=60)
                                GGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTTCTCTATTCGAAAATAATTAATTTACATGAGCCAG                                                                                                                       >  SRR19973070.1726332/1‑150 (MQ=60)
                                       CAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTGACGCTATTCGAAAAAAATGAATTTGCATGGGCCAGACGCACC                                                                                                                >  SRR19973070.2673751/1‑150 (MQ=60)
                                       CAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGAAGCTATGCGAAAATAAATAATTGCCAAGAGCCACAAGCAGC                                                                                                                >  SRR19973070.2670708/1‑150 (MQ=60)
                                         GGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCAC                                                                                                              <  SRR19973070.835389/150‑1 (MQ=60)
                                         GGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATTCGAAAATAAAGAATTGCCATGATCCACAAGCAGCAC                                                                                                              >  SRR19973070.1394036/1‑150 (MQ=60)
                                           CTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAT                                                                                                            <  SRR19973070.2915504/150‑1 (MQ=60)
                                               ATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTTAGATATGCGAAAATAATGAATTGGCATGAGCCAGAagcaccacaataat                                                                                                        >  SRR19973070.2140923/1‑136 (MQ=60)
                                               ATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCAATGACTCTATGCGAAAATAATGAATTGCCATGAGCCCGACGCACCACATTCAT                                                                                                        >  SRR19973070.2233565/1‑150 (MQ=60)
                                                       CCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATGACTCTATTCGAAAAAAATGAATTTCCAAGAGCCAcaagcagcaacataattcattcga                                                                                                >  SRR19973070.1317461/1‑126 (MQ=60)
                                                                AACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTGAATCTATTCGAAAATAATTAATTTCGATGAGCCACACGCAGCACAatcattgattagacgtggtgcg                                                                                       >  SRR19973070.1621785/1‑128 (MQ=60)
                                                                     TATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTT                                                                                  <  SRR19973070.1757533/150‑1 (MQ=60)
                                                                     TATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTT                                                                                  <  SRR19973070.1757540/150‑1 (MQ=60)
                                                                     TATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTT                                                                                  <  SRR19973070.3017906/150‑1 (MQ=60)
                                                                       TTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTAT                                                                                <  SRR19973070.1871920/150‑1 (MQ=60)
                                                                       TTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATGCGAAAAAAATGAATTTCCATGAGCCAAACGGAGCACAATCTTGgcatcgacctggggcgccgttat                                                                                >  SRR19973070.3013784/1‑127 (MQ=60)
                                                                       TTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTAT                                                                                >  SRR19973070.3013871/1‑150 (MQ=60)
                                                                                    TTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAG                                                                   <  SRR19973070.250615/150‑1 (MQ=60)
                                                                                              GCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTT                                                         <  SRR19973070.2297650/150‑1 (MQ=60)
                                                                                              GCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTT                                                         <  SRR19973070.2297645/150‑1 (MQ=60)
                                                                                               CGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGTGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTG                                                        <  SRR19973070.817064/150‑1 (MQ=60)
                                                                                               CGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTG                                                        <  SRR19973070.2587816/150‑1 (MQ=60)
                                                                                                        TGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                               <  SRR19973070.3041973/150‑1 (MQ=60)
                                                                                                         GCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACG                                              <  SRR19973070.126446/150‑1 (MQ=60)
                                                                                                                   GACTTGATCCACACAGTCGAGTTTTTTCATTGACGCTATGCGAAAATAAATAATTTCCATGTGCCCGACGCAGCCCATTCTTGGATTCGACGTGGGGCGCCGTTTTTTATCACCACCACGAAAACCCATGTCCATAGACGCCCCATACAC                                    >  SRR19973070.532540/1‑150 (MQ=44)
                                                                                                                     CTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAT                                  <  SRR19973070.2120227/150‑1 (MQ=60)
                                                                                                                     CTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAT                                  <  SRR19973070.1170703/150‑1 (MQ=60)
                                                                                                                     CTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAT                                  <  SRR19973070.1334782/150‑1 (MQ=60)
                                                                                                                     CTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAT                                  <  SRR19973070.2120235/150‑1 (MQ=60)
                                                                                                                     CTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAT                                  <  SRR19973070.1052315/150‑1 (MQ=60)
                                                                                                                      TTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATG                                 <  SRR19973070.2589632/150‑1 (MQ=60)
                                                                                                                             ACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACA                          <  SRR19973070.3151381/150‑1 (MQ=60)
                                                                                                                                 AGTCGATTTTTATCATTGACTCTATTCGAAAAAAATGAATTGCCATGAGCCAGACGCAGCACATTCTTTCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAAAGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAA                      >  SRR19973070.3213967/1‑150 (MQ=60)
                                                                                                                                 AGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAA                      >  SRR19973070.837810/1‑150 (MQ=60)
                                                                                                                                  GTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAA                     >  SRR19973070.1936586/1‑150 (MQ=60)
                                                                                                                                   TCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAC                    <  SRR19973070.1180440/150‑1 (MQ=60)
                                                                                                                                    CGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACC                   <  SRR19973070.2207694/150‑1 (MQ=60)
                                                                                                                                       TTTTTATCATGGCCTCTATGCGAAAATAATGAATTGACATGAGCCAGACGCAGCAAATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGACTTGTCCATAGACGCCCCTTCCACATGCGCCACACGAAACCTCT                <  SRR19973070.2204523/150‑1 (MQ=60)
                                                                                                                                              CATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAG         <  SRR19973070.907019/150‑1 (MQ=60)
                                                                                                                                              CATTGACTATATGCGAAAATAATGAATTGACATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCATTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAG         <  SRR19973070.2571077/150‑1 (MQ=60)
                                                                                                                                               ATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT        >  SRR19973070.751294/1‑150 (MQ=60)
                                                                                                                                               ATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT        >  SRR19973070.751407/1‑150 (MQ=60)
                                                                                                                                               ATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT        >  SRR19973070.83062/1‑150 (MQ=60)
                                                                                                                                                TTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG       <  SRR19973070.2942278/150‑1 (MQ=60)
                                                                                                                                                TTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG       <  SRR19973070.2942449/150‑1 (MQ=60)
                                                                                                                                                  GACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC     >  SRR19973070.658035/1‑150 (MQ=60)
                                                                                                                                                    CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC   >  SRR19973070.2636826/1‑150 (MQ=60)
                                                                                                                                                    CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC   >  SRR19973070.2637026/1‑150 (MQ=60)
                                                                                                                                                     TCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACA  <  SRR19973070.1382158/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACA  >  CP009273/2004384‑2004682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑