Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,004,533 G→T 100% T151T (ACG→ACT)  yedL → putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,004,5330GT96.4% 465.7 / 9.5 169T151T (ACG→ACT) yedLputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (57/104);  total (60/109)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 173 total aligned reads displayed.

CAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG  >  CP009273/2004386‑2004677
                                                                                                                                                   |                                                                                                                                                
cAGCTGCCCTCCTCGCCTGAGCTTAGAAACGTGTACACCTGCAGGATTTACGCCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                                <  2:2967728/150‑1 (MQ=255)
cAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                                <  1:4747386/150‑1 (MQ=255)
 aGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                               >  1:323154/1‑150 (MQ=255)
 aGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                               <  2:4154828/150‑1 (MQ=255)
  gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAt                                                                                                                                              <  1:728850/150‑1 (MQ=255)
  gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAt                                                                                                                                              <  1:4190885/150‑1 (MQ=255)
   aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                             >  2:683234/1‑150 (MQ=255)
   aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                             <  2:280834/150‑1 (MQ=255)
   aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                             <  1:842061/150‑1 (MQ=255)
   aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                             <  2:3536647/150‑1 (MQ=255)
   aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                             <  1:2195034/150‑1 (MQ=255)
    tGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGc                                                                                                                                            <  2:1740084/150‑1 (MQ=255)
     gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGTATTTACGGCCTGCCATCAGCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCg                                                                                                                                           <  1:4130434/150‑1 (MQ=255)
     gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCg                                                                                                                                           <  1:4130258/150‑1 (MQ=255)
     gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCg                                                                                                                                           <  1:2391401/150‑1 (MQ=255)
      ccTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGa                                                                                                                                          <  1:675754/150‑1 (MQ=255)
           aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaata                                                                                                                                     <  1:1542939/150‑1 (MQ=255)
            tCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaataa                                                                                                                                    <  2:2780431/150‑1 (MQ=255)
            tCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAaataa                                                                                                                                    >  1:329994/1‑150 (MQ=255)
                      ttAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCa                                                                                                                          >  1:1217653/1‑150 (MQ=255)
                      ttAGAAACGGGTACACAGACTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCa                                                                                                                          <  1:5105066/150‑1 (MQ=255)
                         gAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGa                                                                                                                       >  2:3041943/1‑150 (MQ=255)
                          aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAAAAAAGAATTGCCATGAg                                                                                                                      >  2:1535711/1‑150 (MQ=255)
                           aaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGc                                                                                                                     >  1:692633/1‑150 (MQ=255)
                           aaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGc                                                                                                                     <  2:4690503/150‑1 (MQ=255)
                           aaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGc                                                                                                                     <  1:4880627/150‑1 (MQ=255)
                            aCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGcc                                                                                                                    <  1:1695301/150‑1 (MQ=255)
                            aCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGcc                                                                                                                    <  1:3139418/150‑1 (MQ=255)
                            aCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGcc                                                                                                                    <  2:3261482/150‑1 (MQ=255)
                             cGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCa                                                                                                                   <  1:3791028/150‑1 (MQ=255)
                              gggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAg                                                                                                                  <  1:1273429/150‑1 (MQ=255)
                                 tACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACg                                                                                                               <  2:1903082/150‑1 (MQ=255)
                                   cacaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACgca                                                                                                             <  2:4457987/150‑1 (MQ=255)
                                    acaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACgcag                                                                                                            <  1:2332686/150‑1 (MQ=255)
                                        gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGcaca                                                                                                        <  1:454744/150‑1 (MQ=255)
                                         cTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGTCTCTATGCGAAAATAATGTATTGGCCTTAGCCAGACGCAGcacaa                                                                                                       >  1:3539657/1‑149 (MQ=255)
                                          tGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACTATATTCGAAAATAATGAATTGCCAAGAGCCAGAAGCAGcacaat                                                                                                      >  1:723895/1‑148 (MQ=255)
                                            gATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCt                                                                                                    <  2:4754332/150‑1 (MQ=255)
                                              tttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTg                                                                                                  <  2:2288141/150‑1 (MQ=255)
                                                tACGGCCTGCCATCAAATTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCa                                                                                                <  1:3218963/150‑1 (MQ=255)
                                                   ggCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTc                                                                                             <  1:230848/150‑1 (MQ=255)
                                                   ggCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTc                                                                                             <  2:2600766/150‑1 (MQ=255)
                                                   ggCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTc                                                                                             <  1:1234285/150‑1 (MQ=255)
                                                   ggCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTc                                                                                             <  1:1234290/150‑1 (MQ=255)
                                                    gCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCg                                                                                            <  2:110501/150‑1 (MQ=255)
                                                      cTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAc                                                                                          <  2:3798851/150‑1 (MQ=255)
                                                       tGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAca                                                                                         <  2:3935978/150‑2 (MQ=255)
                                                       tGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACg                                                                                         <  1:3690125/150‑1 (MQ=255)
                                                       tGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACg                                                                                         <  1:2697040/150‑1 (MQ=255)
                                                       tGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACg                                                                                         >  2:3535570/1‑150 (MQ=255)
                                                           aTCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCAATTCGAAAATAATGAAATTCCATGAGCCAGACGCAGCACATTCTTTCAATCGACCtgcg                                                                                     >  1:937002/1‑149 (MQ=255)
                                                             cAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgctgc                                                                                   <  2:3713915/150‑1 (MQ=255)
                                                               aCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCCTGAGCCAGACGCAGCCCAATCTTGCATTCGACGTGCTgggc                                                                                 >  1:547469/1‑150 (MQ=255)
                                                                     tttGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttat                                                                           <  1:1499619/150‑1 (MQ=255)
                                                                      ttGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCATTTACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCCGCACAATCTTTCATTCGAAGTGCTGCGCCgttatt                                                                          >  1:1400797/1‑150 (MQ=255)
                                                                       tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTTCATTCGACGTGCTGCGCCtttattt                                                                         >  2:4572594/1‑150 (MQ=255)
                                                                       tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttattt                                                                         <  1:4412404/150‑1 (MQ=255)
                                                                       tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAAAGAATTGCCATGAGCCCGACGCAGCACATTCGTGCATTCGACGTGCTGCGCCtttattt                                                                         >  2:4572460/1‑150 (MQ=255)
                                                                            cATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAc                                                                    >  2:2800171/1‑150 (MQ=255)
                                                                            cATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAc                                                                    >  2:863795/1‑150 (MQ=255)
                                                                             aTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAcc                                                                   <  2:143369/150‑1 (MQ=255)
                                                                             aTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATGACTCTATGCGAAAATAATGAATTTCCAAGAGCCAGACCCAGCACATTATTGCATTCGACCTGCTGCGCCATTATTTATAAcc                                                                   >  1:1602743/1‑150 (MQ=255)
                                                                                 tttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcagca                                                               <  2:2315523/150‑1 (MQ=255)
                                                                                   tCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAgg                                                             <  2:2183427/150‑1 (MQ=255)
                                                                                    cGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATGCGAAAAAAATTAAATTCCATGAGCCAGAAGCAGCACATTCTTTCATTCGACGTGCTGCGCCGTTATTTATAAACAGCAGGa                                                            >  1:1490414/1‑150 (MQ=255)
                                                                                     gCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGaa                                                           >  2:4878172/1‑150 (MQ=255)
                                                                                       tGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAAc                                                         >  2:4507579/1‑150 (MQ=255)
                                                                                            gCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCtt                                                    <  1:1867823/150‑1 (MQ=255)
                                                                                            gCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCtt                                                    >  2:1209584/1‑150 (MQ=255)
                                                                                             cGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTTCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTg                                                   >  2:2805260/1‑150 (MQ=255)
                                                                                              gAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGt                                                  <  1:1048386/150‑1 (MQ=255)
                                                                                                aCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCCGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTcc                                                >  2:1865735/1‑150 (MQ=255)
                                                                                                aCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTcc                                                <  1:653574/150‑1 (MQ=255)
                                                                                                aCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAAAAATGAAATTCCATGATCCCGACGCAGCACAATCTTTCAATAGACGTTCTGCGCCATTATTTATCACCAGCAGGAAACGCCATGtac                                                >  1:2654504/1‑148 (MQ=255)
                                                                                                  cGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCCTTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCATGTCCAt                                              >  2:1289834/1‑150 (MQ=255)
                                                                                                    tttGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAg                                            <  1:3775308/150‑1 (MQ=255)
                                                                                                    tttGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAAAAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAg                                            >  2:2289837/1‑150 (MQ=255)
                                                                                                      tGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAc                                          <  1:3145004/150‑1 (MQ=255)
                                                                                                        cTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGc                                        <  2:3211628/150‑1 (MQ=255)
                                                                                                        cTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCAGGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGc                                        >  2:2378616/1‑150 (MQ=255)
                                                                                                            ttATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCt                                    <  1:4363556/150‑1 (MQ=255)
                                                                                                             tATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCtt                                   <  1:922630/150‑1 (MQ=255)
                                                                                                             tATCGACTTGATCCACACAGTCGATTTTTATAATTGACTATAATCGAAAAAAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCAATCGACGTGCAGCGCCGTTATTTATCACCAGCAGGCAAAGCCGGGGCCCTAGACGCCCCtt                                   >  2:460516/1‑150 (MQ=255)
                                                                                                              aTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTc                                  <  2:2035304/150‑1 (MQ=255)
                                                                                                               tCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTcc                                 <  2:1830493/150‑1 (MQ=255)
                                                                                                               tCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTcc                                 <  2:676349/150‑1 (MQ=255)
                                                                                                                  aCTTGATCCACACAGTCGATTTTTATCATTGACTCTATTCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTATTGCAATAGACGTGCTGCGCCTTTATTTATAACCAGCAGGAAACGCCGTGTCCATATAAGCCCCATCcaca                              >  1:1127949/1‑150 (MQ=255)
                                                                                                                     tGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATgc                           <  1:435093/150‑1 (MQ=255)
                                                                                                                        tCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGcc                        <  2:4475438/150‑1 (MQ=255)
                                                                                                                         ccACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCGTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACCTGCGCca                       >  2:4261922/1‑150 (MQ=255)
                                                                                                                         ccACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACCCAGCACATTCTTGCATTAGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCca                       >  2:1804441/1‑150 (MQ=255)
                                                                                                                           acacaGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCcaca                     <  1:5070464/150‑1 (MQ=255)
                                                                                                                              caGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGa                  <  2:4699858/150‑1 (MQ=255)
                                                                                                                              caGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGa                  <  2:723895/150‑1 (MQ=255)
                                                                                                                                  cGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAcc              <  2:4589380/150‑1 (MQ=255)
                                                                                                                                  cGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAcc              <  2:2171072/150‑1 (MQ=255)
                                                                                                                                     tttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctct           <  2:3899181/150‑1 (MQ=255)
                                                                                                                                           tCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa     <  1:4315505/150‑1 (MQ=255)
                                                                                                                                              ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTg  >  1:2817001/1‑150 (MQ=255)
                                                                                                                                              ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTg  <  2:1936224/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG  >  CP009273/2004386‑2004677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC  >  CP009273/2004384‑2004681
                                                                                                                                                     |                                                                                                                                                    
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                      <  SRR19973063.4556421/150‑1 (MQ=60)
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                      >  SRR19973063.1942275/1‑150 (MQ=60)
  CAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCT                                                                                                                                                    <  SRR19973063.4762504/150‑1 (MQ=60)
   AGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTA                                                                                                                                                   >  SRR19973063.324095/1‑150 (MQ=60)
    GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAT                                                                                                                                                  <  SRR19973063.4204265/150‑1 (MQ=60)
    GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAT                                                                                                                                                  <  SRR19973063.730993/150‑1 (MQ=60)
    GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAT                                                                                                                                                  <  SRR19973063.730971/150‑1 (MQ=60)
     ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATG                                                                                                                                                 <  SRR19973063.844494/150‑1 (MQ=60)
     ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATG                                                                                                                                                 <  SRR19973063.2201929/150‑1 (MQ=60)
       GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGTATTTACGGCCTGCCATCAGCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCG                                                                                                                                               <  SRR19973063.4143651/150‑1 (MQ=60)
       GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCG                                                                                                                                               <  SRR19973063.4143475/150‑1 (MQ=60)
       GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCG                                                                                                                                               <  SRR19973063.2399053/150‑1 (MQ=60)
        CCTTCATCGCCTGAGCTTAGAAACGGGTACACTGGCTGGATTTACGGCCTGCCATCATCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGA                                                                                                                                              <  SRR19973063.677699/150‑1 (MQ=60)
        CCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATTCGA                                                                                                                                              >  SRR19973063.2295444/1‑150 (MQ=60)
        CCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGA                                                                                                                                              <  SRR19973063.2957408/150‑1 (MQ=60)
        CCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGA                                                                                                                                              <  SRR19973063.677728/150‑1 (MQ=60)
             ATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATA                                                                                                                                         <  SRR19973063.1547670/150‑1 (MQ=60)
             ATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATGCGAAAATA                                                                                                                                         >  SRR19973063.3407274/1‑150 (MQ=60)
              TCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAATAA                                                                                                                                        >  SRR19973063.330952/1‑150 (MQ=60)
                GCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATG                                                                                                                                      <  SRR19973063.4527435/150‑1 (MQ=60)
                  CTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAA                                                                                                                                    <  SRR19973063.2550273/150‑1 (MQ=60)
                    GAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGAAAATAATGAATT                                                                                                                                  >  SRR19973063.1601801/1‑150 (MQ=60)
                      GCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATTCGAAAATAATGAAattc                                                                                                                                >  SRR19973063.631085/1‑146 (MQ=60)
                        TTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCA                                                                                                                              >  SRR19973063.161012/1‑150 (MQ=60)
                        TTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCA                                                                                                                              >  SRR19973063.1221300/1‑150 (MQ=60)
                        TTAGAAACGGGTACACAGACTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCA                                                                                                                              <  SRR19973063.5121495/150‑1 (MQ=60)
                         TAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCAT                                                                                                                             <  SRR19973063.1493430/150‑1 (MQ=60)
                             AACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGC                                                                                                                         >  SRR19973063.694671/1‑150 (MQ=60)
                             AACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGC                                                                                                                         <  SRR19973063.4896366/150‑1 (MQ=60)
                              ACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCC                                                                                                                        <  SRR19973063.3149621/150‑1 (MQ=60)
                              ACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCC                                                                                                                        <  SRR19973063.1700466/150‑1 (MQ=60)
                              ACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCC                                                                                                                        <  SRR19973063.3149514/150‑1 (MQ=60)
                               CGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCA                                                                                                                       <  SRR19973063.3803117/150‑1 (MQ=60)
                                GGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAG                                                                                                                      <  SRR19973063.1277220/150‑1 (MQ=60)
                                  GTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGAC                                                                                                                    <  SRR19973063.920538/150‑1 (MQ=60)
                                      ACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAG                                                                                                                <  SRR19973063.2340229/150‑1 (MQ=60)
                                      ACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAG                                                                                                                <  SRR19973063.2340148/150‑1 (MQ=60)
                                       CAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTATATGCGAAAATAATTAATTTCCAAGAGCCAGACCCAGC                                                                                                               >  SRR19973063.4827446/1‑150 (MQ=60)
                                       CAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGTCTATATGCGAAAAAAATGAATTTCCAAGATCCAGACGCAGC                                                                                                               >  SRR19973063.4827448/1‑150 (MQ=60)
                                         GGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCAC                                                                                                             >  SRR19973063.123312/1‑150 (MQ=60)
                                          GCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACA                                                                                                            <  SRR19973063.456021/150‑1 (MQ=60)
                                           CTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGTCTCTATGCGAAAATAATGTATTGGCCTTAGCCAGACGCAGCACAA                                                                                                           >  SRR19973063.3550997/1‑150 (MQ=60)
                                            TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACTATATTCGAAAATAATGAATTGCCAAGAGCCAGAAGCAGCACAAT                                                                                                          >  SRR19973063.726021/1‑150 (MQ=60)
                                                  TACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGAATCGATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCA                                                                                                    >  SRR19973063.678325/1‑150 (MQ=60)
                                                  TACGGCCTGCCATCAAATTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCA                                                                                                    <  SRR19973063.3229309/150‑1 (MQ=60)
                                                   ACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCAT                                                                                                   <  SRR19973063.1397069/150‑1 (MQ=60)
                                                    CGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTGACAATATTCGAAAATAATGAATTtcactgacccagaagcagcaaaatattgcatt                                                                                                  >  SRR19973063.4996632/1‑118 (MQ=60)
                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTC                                                                                                 <  SRR19973063.231540/150‑1 (MQ=60)
                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTC                                                                                                 <  SRR19973063.1237966/150‑1 (MQ=60)
                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTC                                                                                                 <  SRR19973063.1237971/150‑1 (MQ=60)
                                                        CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTAATCTATTCGAAAATAATTAATTTCCATGAGCCCGACGCAGCACATTATTTCAATCGAC                                                                                              >  SRR19973063.1532645/1‑150 (MQ=60)
                                                         TGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACG                                                                                             <  SRR19973063.3701939/150‑1 (MQ=60)
                                                         TGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACG                                                                                             <  SRR19973063.2705736/150‑1 (MQ=60)
                                                           CCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTG                                                                                           <  SRR19973063.2073933/150‑1 (MQ=60)
                                                             ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCAATTCGAAAATAATGAAATTCCATGAGCCAGACGCAGCACATTCTTtcaatcgacctgcg                                                                                         >  SRR19973063.939795/1‑136 (MQ=60)
                                                               CAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGC                                                                                       <  SRR19973063.4179158/150‑1 (MQ=60)
                                                                 ACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCCTGAGCCAGACGCAGCCCAATCTTGCATTCGACGTGCTGGGC                                                                                     >  SRR19973063.549088/1‑150 (MQ=60)
                                                                      ATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGTGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTA                                                                                <  SRR19973063.5102342/150‑1 (MQ=60)
                                                                      ATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACAATCTTGCATTAGACGTGCTGCGCCGTTA                                                                                >  SRR19973063.4107889/1‑150 (MQ=60)
                                                                       TTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTAT                                                                               <  SRR19973063.1504211/150‑1 (MQ=60)
                                                                        TTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCATTTACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCCGCACAATCTTTCATTCGAAGTGCTGCGCCGTTATT                                                                              >  SRR19973063.1405035/1‑150 (MQ=60)
                                                                         TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                             <  SRR19973063.4426558/150‑1 (MQ=60)
                                                                         TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                             <  SRR19973063.3188878/150‑1 (MQ=60)
                                                                               ATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATGACTCTATGCGAAAATAATGAATTTCCAAGAGCCAGACCCAGCACATTATTGCATTCGACCTGCTGCGCCATTATTTATAACC                                                                       >  SRR19973063.1607653/1‑150 (MQ=60)
                                                                                TGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCA                                                                      <  SRR19973063.3788828/150‑1 (MQ=60)
                                                                                TGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCA                                                                      <  SRR19973063.559068/150‑1 (MQ=60)
                                                                                      CGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATGCGAAAAAAATTAAATTCCATGAGCCAGAAGCAGCACATTCTTTCATTCGACGTGCTGCGCCGTTATTTATAAACAGCAGGA                                                                >  SRR19973063.1494980/1‑150 (MQ=60)
                                                                                       GCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAA                                                               <  SRR19973063.1243679/150‑1 (MQ=60)
                                                                                              GCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTT                                                        <  SRR19973063.1873659/150‑1 (MQ=60)
                                                                                                GAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGT                                                      <  SRR19973063.1051560/150‑1 (MQ=60)
                                                                                                 AACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTC                                                     <  SRR19973063.4325827/150‑1 (MQ=60)
                                                                                                  ACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCCTTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGTAAAGCCTTGTCC                                                    >  SRR19973063.2662999/1‑150 (MQ=60)
                                                                                                  ACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCC                                                    <  SRR19973063.655489/150‑1 (MQ=60)
                                                                                                  ACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAAAAATGAAATTCCATGATCCCGACGCAGCACAATCTTTCAATAGACGTTCTGCGCCATTATTTATCACCAGCAGGAAACGCCATGTAC                                                    >  SRR19973063.2662993/1‑150 (MQ=60)
                                                                                                  ACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTGCAATAGACGTGCTGCGCCGTTATTTATCACCAGCAGGAAACGCCTTGTCC                                                    >  SRR19973063.1179445/1‑150 (MQ=60)
                                                                                                      TTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAG                                                <  SRR19973063.3787365/150‑1 (MQ=60)
                                                                                                        TGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                              <  SRR19973063.3155115/150‑1 (MQ=60)
                                                                                                              TTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCT                                        <  SRR19973063.4377516/150‑1 (MQ=60)
                                                                                                               TATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTT                                       <  SRR19973063.925364/150‑1 (MQ=60)
                                                                                                                    ACTTGATCCACACAGTCGATTTTTATCATTGACTCTATTCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTATTGCAATAGACGTGCTGCGCCTTTATTTATAACCAGCAGGAAACGCCGTGTCCATATAAGCCCCATCCACA                                  >  SRR19973063.1131369/1‑150 (MQ=60)
                                                                                                                      TTGATCCACACAGTCGATTTTTATAATTTACTCTATTCGCAAATAAAGAATTTCCCTGAGCCACACGCCGCCCAATCGTGCATTCGACCTGCTGCGCCTTTATTTATAAACACCACGAAACCCCGTGTCCATAGACGCCCCTTCCACAAG                                >  SRR19973063.3622296/1‑150 (MQ=35)
                                                                                                                       TGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGC                               <  SRR19973063.436302/150‑1 (MQ=60)
                                                                                                                       TGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGC                               <  SRR19973063.436275/150‑1 (MQ=60)
                                                                                                                        GATCCACACAGTCGATTTTTATCATTGACTCAATTCGAAAAAAATTAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTAGACGTGCTGCGCCGTTATTTTTCACCAGCAGGAAACGCCGTGTCCATAGACGCCCCATACACAAGCG                              >  SRR19973063.4607611/1‑150 (MQ=60)
                                                                                                                             ACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACA                         <  SRR19973063.5086774/150‑1 (MQ=60)
                                                                                                                                    CGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACC                  <  SRR19973063.324970/150‑1 (MQ=60)
                                                                                                                                             TCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA         >  SRR19973063.3763464/1‑150 (MQ=60)
                                                                                                                                             TCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA         <  SRR19973063.4329228/150‑1 (MQ=60)
                                                                                                                                               ATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCTGT       <  SRR19973063.4398817/150‑1 (MQ=60)
                                                                                                                                                TTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG      >  SRR19973063.2826056/1‑150 (MQ=60)
                                                                                                                                                TTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG      <  SRR19973063.794702/150‑1 (MQ=60)
                                                                                                                                                    CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC  <  SRR19973063.5125925/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC  >  CP009273/2004384‑2004681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑