Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F394 I0 R1	1342	215.0	7134094	96.2%	6862998	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,004,533	G→T	100%	T151T (ACG→ACT)	yedL →	putative acyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,004,533	0	G	T	96.3%	471.6 / 7.0	190	T151T (ACG→ACT)	yedL	putative acyl transferase
Reads supporting (aligned to +/- strand): ref base G (7/1); new base T (78/98); total (91/99)
Fisher's exact test for biased strand distribution p-value = 2.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG  >  CP009273/2004388‑2004677

 aTGCCTTCATCGCCTGAGCTTAGAAACTGGTACACAGGCTGTATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                                <  2:851991/148‑1 (MQ=255)

 aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTa                                                                                                                                                >  2:2271967/1‑148 (MQ=255)

  tGCCTTCATCGCCTTAGCTTAGAAACGGGTACACAGGCTGGTTTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAt                                                                                                                                               <  2:2777350/148‑1 (MQ=255)

   gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACTCTATg                                                                                                                                              >  1:1370951/1‑148 (MQ=255)

   gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGTCTCTATg                                                                                                                                              >  2:1749746/1‑148 (MQ=255)

   gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                              >  2:2629800/1‑148 (MQ=255)

    ccTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGc                                                                                                                                             >  2:2166032/1‑148 (MQ=255)

      ttCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATGACTCTATGCGa                                                                                                                                           >  2:1660798/1‑148 (MQ=255)

        cATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaaa                                                                                                                                         <  2:334319/148‑1 (MQ=255)

          tCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaat                                                                                                                                       <  2:254194/148‑1 (MQ=255)

          tCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaat                                                                                                                                       <  1:2689197/148‑1 (MQ=255)

            gCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTTTGCGCaaaaaa                                                                                                                                     >  2:2993105/1‑148 (MQ=255)

             ccTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaataat                                                                                                                                    <  1:3075009/148‑1 (MQ=255)

             ccTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACACTATGCGAAaataat                                                                                                                                    >  2:292814/1‑148 (MQ=255)

                gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGa‑a                                                                                                                                <  2:289795/148‑1 (MQ=255)

                   cTTAGAAACGGGTACACAGGCTGGATGTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCTCTGATTGCGAAACGTTTGCTGATTTTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTg                                                                                                                             <  1:2038666/148‑1 (MQ=255)

                    ttAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACTCTATTCGAAAAAAATGA‑Atttc                                                                                                                            >  1:3128521/1‑146 (MQ=255)

                    ttAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGc                                                                                                                            <  1:1450309/148‑1 (MQ=255)

                       gAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCAt                                                                                                                         <  1:3300466/148‑1 (MQ=255)

                       gAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCAt                                                                                                                         <  2:2648317/148‑1 (MQ=255)

                           cGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGc                                                                                                                     <  1:2178709/148‑1 (MQ=255)

                           cGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTGATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGc                                                                                                                     <  2:3002897/148‑1 (MQ=255)

                               tACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGa                                                                                                                 <  2:2223937/148‑1 (MQ=255)

                               tACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGa                                                                                                                 <  2:2027938/148‑1 (MQ=255)

                                  acaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACgc                                                                                                              <  2:2912012/148‑1 (MQ=255)

                                     ggCTGGATTTACGGCCTGCTATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTTATCTATTCGAAAATAATTA‑ATTTCCATGATCCACACgcagc                                                                                                           >  1:508880/1‑148 (MQ=255)

                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATTCGAAAATAAAGA‑ATTTCCATGAGCCAGACgcagca                                                                                                          >  1:2061669/1‑148 (MQ=255)

                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGTATCTATTCGAAAATAATTA‑ATTGCCATGAGCCAGACgcagca                                                                                                          >  2:1396323/1‑148 (MQ=255)

                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCAATGAGAAAATAAAGACATTGCC‑TGAGCCAGACgcccca                                                                                                          >  1:881220/1‑144 (MQ=255)

                                        tGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGcaca                                                                                                        <  2:3298670/148‑1 (MQ=255)

                                            tttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATTA‑ATTGGCAAGAGCCAGACGCAGcacaatat                                                                                                    >  2:1259514/1‑144 (MQ=255)

                                               aCGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGc                                                                                                 <  1:184736/148‑1 (MQ=255)

                                                cGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTTCTCTATGAGAAAATAATGA‑ATTTCCATGAGCCAGAAGCAGCACAATCTTGCa                                                                                                >  2:977265/1‑148 (MQ=255)

                                                 ggCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCAt                                                                                               <  1:1322858/148‑1 (MQ=255)

                                                  gCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCAtt                                                                                              <  1:912553/148‑1 (MQ=255)

                                                    cTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCg                                                                                            <  1:2393274/148‑1 (MQ=255)

                                                       ccATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACg                                                                                         <  2:986474/148‑1 (MQ=255)

                                                         aTCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtg                                                                                       <  2:2744469/148‑1 (MQ=255)

                                                             aCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTTTAATTGACACTATTCGAAAAAAAATA‑ATTTGCAATTGCCACACCCAGCACAATCATTCATTAGACGtggtgc                                                                                   >  1:2257866/1‑148 (MQ=255)

                                                                ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTAATAAATTCGAAAATAAAGA‑ATTTCCATGAGCCCGAAGCAGCACATTCTTGCATTAGACGTGCTGCGcc                                                                                >  2:928380/1‑148 (MQ=255)

                                                                ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGcc                                                                                <  1:780185/148‑1 (MQ=255)

                                                                ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGcc                                                                                <  1:2357221/148‑1 (MQ=255)

                                                                ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTATATTCGAAAATAATGA‑ATTTGCATTATCCAGACGCAGCACAATATTTGATTAGAACTGGTGCGcc                                                                                >  1:3473420/1‑148 (MQ=255)

                                                                 tATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCt                                                                               <  2:1956466/148‑1 (MQ=255)

                                                                   tttGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCttt                                                                             <  1:1689533/148‑1 (MQ=255)

                                                                    ttGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTCATTTAATCTATTCGAAAATAATGA‑ATTTCCATGTGCCAGACGCAGCACAATCTTGCATTCGACGTGGGGCGCCttta                                                                            >  1:3243823/1‑148 (MQ=255)

                                                                    ttGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACAATCTTTCAATCGACGTGCTGCGCCttta                                                                            >  2:797442/1‑148 (MQ=255)

                                                                     tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttat                                                                           <  1:3446362/148‑1 (MQ=255)

                                                                       aaGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttattt                                                                         <  1:3559469/148‑1 (MQ=255)

                                                                       aaGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttattt                                                                         <  2:2156822/148‑1 (MQ=255)

                                                                         gCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTttatttat                                                                       <  1:791817/148‑1 (MQ=255)

                                                                          cATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATTA‑AATTGCATGAGCCAGACGCAGCACATTATTGCATTCGACGTGCTGCGCCTTTATTTATc                                                                      >  2:2549099/1‑148 (MQ=255)

                                                                           aTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCa                                                                     <  2:749807/148‑1 (MQ=255)

                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGAAAAAAATGA‑ATTGCCAAGAGCCCGACGCAGCACAATCTTGCAGTAGACGTGCTGCGCCGTTATTTATGAc                                                                    >  2:471607/1‑148 (MQ=255)

                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAc                                                                    <  1:1999932/148‑1 (MQ=255)

                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCGTTATTTATCAc                                                                    >  2:1962742/1‑148 (MQ=255)

                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAATTTACTCTATTCGAAAATAATTA‑ATTTCCCTGAGCCAGACGCACCACAATCATTCATTCGACGGGCTGCGCCGTTTTTTATCAc                                                                    >  1:3522743/1‑148 (MQ=255)

                                                                             ggTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTGATTTAGAATATTCGAAAAAAAAGA‑ATTTGCATGAGCCCCACGCAGCCCAATAATGGATTGGAGCTGGTGGGCCCttttttataaac                                                                   >  1:3394910/1‑144 (MQ=255)

                                                                              gTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGAAGCAGCAAATTCTTGCATTCGACGTGCTGCGCCTttatttatgaaaa                                                                  >  2:3047242/1‑143 (MQ=255)

                                                                                ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTTCTCTAATCGAAAAAAAAGA‑ATTGCCAAGAGCCAGAAGCAGCACATTCTTGCATTAGACGTGCTGCGCCCTTATTTATCACcagc                                                                >  2:1872169/1‑148 (MQ=255)

                                                                                ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcagc                                                                <  2:1635788/148‑1 (MQ=255)

                                                                                ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACAATCTTGCATTCGACGTGCTGCGCCGTTATTTATAACcagc                                                                >  2:153527/1‑148 (MQ=255)

                                                                                       aTTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCGTGCCTTCGGCGTGCTGCGCCTTTATTTTTCACCAGCAGGAAAc                                                         >  2:2011959/1‑148 (MQ=255)

                                                                                             aaCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATTCGAAAATAATGA‑ATTTCCATGAGCCAGACGCAGCACAATCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCACCAGGAAAACCCTTg                                                   >  2:2867509/1‑148 (MQ=255)

                                                                                             aaCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAAGCGAAAATAATGA‑ATTGCCAAGAGCCAGACGCAGCACATTCTTGCAATCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTg                                                   >  2:1708794/1‑148 (MQ=255)

                                                                                             aaCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACACTATGCGCAAATAATGA‑ATTTCCATGAGCCACACGCAGCACATTCTTGCATTCGACCTGCAGCGCCTTTATTTATCACCACCAGGAAACCCCGTg                                                   >  2:235060/1‑148 (MQ=255)

                                                                                             aaCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATTACGCTATTCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCATGCAATCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACAccatt                                                   >  2:3217488/1‑145 (MQ=255)

                                                                                             aaCAGTTTGCTGATTATCGACTTGATCCACACAGTAGATTTTTATCATTGAATCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACTTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTg                                                   <  1:3108725/148‑1 (MQ=255)

                                                                                               ccGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTc                                                 <  2:3243823/148‑1 (MQ=255)

                                                                                                cGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTcc                                                <  1:860475/148‑1 (MQ=255)

                                                                                                  tttGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATGAATCTATGCGAAAATAATGA‑AATTCCCTGAGCCCGACGCAGCACATTATTGCATTCGACCTGCTGCGCCGTTATTTATCACCAGCAGGAAACCCCTTTTccca                                              >  2:1627066/1‑146 (MQ=255)

                                                                                                    tGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAg                                            <  1:16369/148‑1 (MQ=255)

                                                                                                    tGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAg                                            <  1:714765/148‑1 (MQ=255)

                                                                                                     gCTGATTATCGACTTGATCCACACAGTCGTTTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGa                                           <  1:3492403/148‑1 (MQ=255)

                                                                                                         aTTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGcc                                       <  1:2762896/148‑1 (MQ=255)

                                                                                                          ttATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCTAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACTTTCTTGCTTTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGccc                                      <  2:40572/148‑1 (MQ=255)

                                                                                                           tATCGACTTGATCCACACAGTCGATTTTTATCATTTACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACAATCTTGGATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAAAGCCTTGTCCATAGAACcccc                                     >  2:521291/1‑148 (MQ=255)

                                                                                                           tATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGcccc                                     <  1:2993105/148‑1 (MQ=255)

                                                                                                            aTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCt                                    <  1:3272561/148‑1 (MQ=255)

                                                                                                             tCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCtt                                   <  1:1149388/148‑1 (MQ=255)

                                                                                                                  ttGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTTCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAACAGCAGGAAAAGCCTTGTCCAAAGTCGCCCCTTCcaca                              >  2:2682638/1‑148 (MQ=255)

                                                                                                                  ttGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcaca                              <  1:454285/148‑1 (MQ=255)

                                                                                                                      tCCACACAGTCGATTTTTTTAATTGACTCTATTCGAAAATAATGA‑ATTTCCATGAGCCAGACGCCGCACATTCTTTCATTCGAAGTGGTGCGCCTTTATTTATAACCAGCAGGAAACGCCTTGTCCATAGACGCCCCATCCACAAgcg                          >  2:568595/1‑148 (MQ=255)

                                                                                                                      tCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATgcg                          <  2:3052574/148‑1 (MQ=255)

                                                                                                                         acacaGTCGATTTTTATCAATTACAAAAAGCGAAAAAAATTA‑AATTCCAAGAGCCAGACGCAGCACAATAATTCAATAGAAGTTCTTCGCCTTTATTTATAAACAGCAGGAAACCCCTTGTCCAAAAAAGCCCCATCCACAATCGCca                       >  2:1168755/1‑148 (MQ=255)

                                                                                                                          cacaGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCAAGAGCCAGACCCAGCACAATCTTGCATTCGACGTGCTGCGCCGTTATTTATAACCACCACGAAACCCCATGTCCAAAGACCCCCCGACCACAAGCGCcac                      >  2:2510327/1‑148 (MQ=255)

                                                                                                                              gTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTTCCATGAGCCAGACGCAGCCCATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGa                  >  1:1189865/1‑148 (MQ=255)

                                                                                                                               tCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaa                 <  1:1574577/148‑1 (MQ=255)

                                                                                                                               tCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaa                 <  1:1295006/148‑1 (MQ=255)

                                                                                                                                cGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaaa                >  1:2627579/1‑148 (MQ=255)

                                                                                                                                   tttttATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct             >  2:157292/1‑148 (MQ=255)

                                                                                                                                     tttATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctct           <  2:639344/148‑1 (MQ=255)

                                                                                                                                       tATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAt         <  2:115155/148‑1 (MQ=255)

                                                                                                                                       tATCATTGACTCTATGCGAAAATAATGA‑AATGACATGAGCCAGACGCAGCACATTCTTGCATACTACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGACTTGTCAATATACGCACCTTCCACATGAGCCACAAGAAACCTCTAt         >  1:3159979/1‑148 (MQ=255)

                                                                                                                                        aTCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAtt        >  2:2944967/1‑148 (MQ=255)

                                                                                                                                          cATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc      >  1:600376/1‑148 (MQ=255)

                                                                                                                                            ttGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg    <  2:3491350/148‑1 (MQ=255)

                                                                                                                                            ttGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg    <  2:3173767/148‑1 (MQ=255)

                                                                                                                                            ttGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg    >  1:867449/1‑148 (MQ=255)

                                                                                                                                             tGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGt   >  2:86053/1‑148 (MQ=255)

GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG  >  CP009273/2004388‑2004677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC  >  CP009273/2004384‑2004679

GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                     <  SRR19973066.657541/150‑1 (MQ=60)

    GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAT                                                                                                                                                 <  SRR19973066.3580591/150‑1 (MQ=60)

     ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATG                                                                                                                                                <  SRR19973066.591490/150‑1 (MQ=60)

     ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATG                                                                                                                                                <  SRR19973066.3120692/150‑1 (MQ=60)

              TCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAA                                                                                                                                       <  SRR19973066.2707146/150‑1 (MQ=60)

                GCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATG                                                                                                                                     <  SRR19973066.3494138/150‑1 (MQ=60)

                 CCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA                                                                                                                                    <  SRR19973066.3095549/150‑1 (MQ=60)

                 CCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA                                                                                                                                    <  SRR19973066.3095556/150‑1 (MQ=60)

                      GCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACTCTATTCGAAAAAAATGA‑ATTTC                                                                                                                              >  SRR19973066.3149407/1‑150 (MQ=60)

                       CTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCC                                                                                                                             <  SRR19973066.1419455/150‑1 (MQ=60)

                       CTTAGAAACGGGTACACAGGCTGGATGTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCTCTGATTGCGAAACGTTTGCTGATTTTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCC                                                                                                                             <  SRR19973066.2052873/150‑1 (MQ=60)

                        TTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCA                                                                                                                            <  SRR19973066.1460604/150‑1 (MQ=60)

                         TAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCAT                                                                                                                           <  SRR19973066.3514157/150‑1 (MQ=60)

                           GAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGA                                                                                                                         <  SRR19973066.3322286/150‑1 (MQ=60)

                               CGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCA                                                                                                                     <  SRR19973066.2193833/150‑1 (MQ=60)

                                       CAGGCTGGATTTACGGCCTGCTATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTTATCTATTCGAAAATAATTA‑ATTTCCATGATCCACACGCAGC                                                                                                             >  SRR19973066.512651/1‑150 (MQ=60)

                                        AGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATTCGAAAATAAAGA‑ATTTCCATGAGCCAGACGCAGCA                                                                                                            >  SRR19973066.2075960/1‑150 (MQ=60)

                                        AGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCAATGAGAAAATAAAGACATTGCC‑TGAGCCAGACGCccca                                                                                                            >  SRR19973066.887486/1‑146 (MQ=60)

                                            TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATT                                                                                                        <  SRR19973066.1331601/150‑1 (MQ=60)

                                             GGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTTATCTATTCGAAAATAATTA‑ATTGGCATGAGCCAGAAGCCGCACATTC                                                                                                       >  SRR19973066.1700605/1‑150 (MQ=60)

                                                   ACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCAT                                                                                                 <  SRR19973066.186347/150‑1 (MQ=60)

                                                    CGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATT                                                                                                <  SRR19973066.3586218/150‑1 (MQ=60)

                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTC                                                                                               <  SRR19973066.2173803/150‑1 (MQ=60)

                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTC                                                                                               <  SRR19973066.1332445/150‑1 (MQ=60)

                                                      GCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCG                                                                                              <  SRR19973066.919045/150‑1 (MQ=60)

                                                        CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAC                                                                                            <  SRR19973066.2409367/150‑1 (MQ=60)

                                                        CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAC                                                                                            <  SRR19973066.2409391/150‑1 (MQ=60)

                                                        CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAC                                                                                            <  SRR19973066.2409803/150‑1 (MQ=60)

                                                        CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAC                                                                                            <  SRR19973066.2265741/150‑1 (MQ=60)

                                                        CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAC                                                                                            <  SRR19973066.460695/150‑1 (MQ=60)

                                                          GCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGT                                                                                          <  SRR19973066.1706370/150‑1 (MQ=60)

                                                          GCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGT                                                                                          <  SRR19973066.2794606/150‑1 (MQ=60)

                                                               CAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTGTATGCGAAAATAATTA‑ATTTCCATGAGCCAGACGCACCACATTATTTCATTCGACGTGCTGC                                                                                     >  SRR19973066.1198951/1‑150 (MQ=60)

                                                               CAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTTTAATTGACACTATTCGAAAAaaaataatttgcaattgccacacccagcacaatcattcattagacgtggtgc                                                                                      >  SRR19973066.2273421/1‑98 (MQ=60)

                                                                  CTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTATATTCGAAAATAATGA‑ATTTGCATTATCCAGACGCAGCACAatatttgattagaactggtgcgcc                                                                                  >  SRR19973066.3496613/1‑126 (MQ=60)

                                                                    TTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTT                                                                                <  SRR19973066.2373213/150‑1 (MQ=60)

                                                                    TTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTT                                                                                <  SRR19973066.785943/150‑1 (MQ=60)

                                                                      ATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTCATTTAATCTATTCGAAAATAATGA‑ATTTCCATGTGCCAGACGCAGCACAATCTTGCATTCGACGTGGGGCGCCTTTA                                                                              >  SRR19973066.3265215/1‑150 (MQ=60)

                                                                       TTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTAT                                                                             <  SRR19973066.1701829/150‑1 (MQ=60)

                                                                         TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                           <  SRR19973066.191902/150‑1 (MQ=60)

                                                                         TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                           <  SRR19973066.3469412/150‑1 (MQ=60)

                                                                           AAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTAT                                                                         <  SRR19973066.3583075/150‑1 (MQ=60)

                                                                             GCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCA                                                                       <  SRR19973066.797636/150‑1 (MQ=60)

                                                                              CATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAATTTACTCTATTCGAAAATAATTA‑ATTTCCCTGAGCCAGACGCACCACAATCATTCATTCGACGGGCTGCGCCGTTTTTTATCAC                                                                      >  SRR19973066.3546192/1‑150 (MQ=60)

                                                                                TGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCA                                                                    <  SRR19973066.2013989/150‑1 (MQ=60)

                                                                                         TGATTGCGAACCGTTTGCTGATTATCGACTTGATCAACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAAC                                                           <  SRR19973066.317140/150‑1 (MQ=60)

                                                                                             TGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCT                                                       <  SRR19973066.792591/150‑1 (MQ=60)

                                                                                              GCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTT                                                      <  SRR19973066.3251674/150‑1 (MQ=60)

                                                                                                 AACAGTTTGCTGATTATCGACTTGATCCACACAGTAGATTTTTATCATTGAATCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACTTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTC                                                   <  SRR19973066.3129489/150‑1 (MQ=60)

                                                                                                    CGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCAT                                                <  SRR19973066.866668/150‑1 (MQ=60)

                                                                                                     GTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATTACACTATTCGAAAATAAAGA‑ATTtcccagagccccccgccccccaaactttcattcgacgtgctgcgccgttatttataaccaccccgcaaccccgtgtcccaa                                               >  SRR19973066.513323/1‑69 (MQ=56)

                                                                                                        TGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                            <  SRR19973066.16624/150‑1 (MQ=60)

                                                                                                        TGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                            <  SRR19973066.720060/150‑1 (MQ=60)

                                                                                                        TGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                            <  SRR19973066.720078/150‑1 (MQ=60)

                                                                                                        TGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                            <  SRR19973066.16591/150‑1 (MQ=60)

                                                                                                         GCTGATTATCGACTTGATCCACACAGTCGTTTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACG                                           <  SRR19973066.3515696/150‑1 (MQ=60)

                                                                                                             ATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCC                                       <  SRR19973066.2781497/150‑1 (MQ=60)

                                                                                                               TATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTT                                     <  SRR19973066.3012944/150‑1 (MQ=60)

                                                                                                                ATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTC                                    <  SRR19973066.3294382/150‑1 (MQ=60)

                                                                                                                ATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTC                                    <  SRR19973066.2739413/150‑1 (MQ=60)

                                                                                                                ATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTC                                    <  SRR19973066.3294037/150‑1 (MQ=60)

                                                                                                                 TCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCC                                   <  SRR19973066.1157586/150‑1 (MQ=60)

                                                                                                                      TTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATG                              <  SRR19973066.457778/150‑1 (MQ=60)

                                                                                                                      TTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATG                              <  SRR19973066.3323220/150‑1 (MQ=60)

                                                                                                                       TGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGC                             <  SRR19973066.154956/150‑1 (MQ=60)

                                                                                                                                CAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTTCCATGAGCCAGACGCAGCCCATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATATACGCCCCTTCCACATGCGCCACAAGA                    >  SRR19973066.1198203/1‑150 (MQ=60)

                                                                                                                                  GTCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAA                  >  SRR19973066.2645287/1‑150 (MQ=60)

                                                                                                                                   TCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAC                 <  SRR19973066.1585925/150‑1 (MQ=60)

                                                                                                                                   TCGATTTTTATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAC                 <  SRR19973066.1304387/150‑1 (MQ=60)

                                                                                                                                         TTTATCATTGACTCTATGCGAAAATAATGA‑AATGACATGAGCCAGACGCAGCACATTCTTGCATACTACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGACTTGTCAATATACGCACCTTCCACATGAGCCACAAGAAACCTCTAT           >  SRR19973066.3181037/1‑150 (MQ=60)

                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC        >  SRR19973066.604651/1‑150 (MQ=60)

                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC        >  SRR19973066.604508/1‑150 (MQ=60)

                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC        <  SRR19973066.799057/150‑1 (MQ=60)

                                                                                                                                              CATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAG      >  SRR19973066.873265/1‑150 (MQ=60)

                                                                                                                                              CATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAG      >  SRR19973066.873671/1‑150 (MQ=60)

                                                                                                                                              CATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAG      >  SRR19973066.876093/1‑150 (MQ=60)

                                                                                                                                                 TGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGA   >  SRR19973066.2389704/1‑150 (MQ=60)

                                                                                                                                                 TGACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGA   >  SRR19973066.2992424/1‑150 (MQ=60)

                                                                                                                                                  GACTCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC  >  SRR19973066.3519775/1‑150 (MQ=60)

GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGA‑ATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC  >  CP009273/2004384‑2004679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`