Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,004,533 G→T 93.4% T151T (ACG→ACT)  yedL → putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,004,5330GT93.4% 422.8 / 17.8 179T151T (ACG→ACT) yedLputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (5/8);  new base T (88/74);  total (97/82)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT  >  CP009273/2004388‑2004676
                                                                                                                                                 |                                                                                                                                               
gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                               <  2:2002858/148‑1 (MQ=255)
gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                               >  2:3500840/1‑148 (MQ=255)
gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                               <  2:1157236/148‑1 (MQ=255)
gATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                               <  2:2002725/148‑1 (MQ=255)
 aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                              <  2:1749772/148‑1 (MQ=255)
 aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                              <  1:1173696/148‑1 (MQ=255)
   gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTGActctctt                                                                                                                                            >  2:781272/1‑145 (MQ=255)
   gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATg                                                                                                                                            <  1:3002323/148‑1 (MQ=255)
         aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaaaa                                                                                                                                      <  2:400974/148‑1 (MQ=255)
         aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCATGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaaaa                                                                                                                                      <  2:1826580/148‑1 (MQ=255)
          tCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGCAaat                                                                                                                                     >  2:1020258/1‑148 (MQ=255)
          tCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATATACTATATTCGAAaat                                                                                                                                     >  2:2796856/1‑148 (MQ=255)
           cGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaata                                                                                                                                    <  2:2276176/148‑1 (MQ=255)
            gCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaataa                                                                                                                                   <  2:1427621/148‑1 (MQ=255)
            gCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaataa                                                                                                                                   <  1:1649734/148‑1 (MQ=255)
              cTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATg                                                                                                                                 <  1:17210/148‑1 (MQ=255)
               tGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGa                                                                                                                                <  1:3348754/148‑1 (MQ=255)
                gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGaa                                                                                                                               >  2:518509/1‑148 (MQ=255)
                gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGaa                                                                                                                               <  1:2722891/148‑1 (MQ=255)
                     tAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATTAATTGcc                                                                                                                          >  2:3370073/1‑148 (MQ=255)
                        aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATg                                                                                                                       <  2:3150460/148‑1 (MQ=255)
                            gggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGcc                                                                                                                   <  1:3526962/148‑1 (MQ=255)
                            gggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGcc                                                                                                                   <  1:2483585/148‑1 (MQ=255)
                             ggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACACTATTCGAAAAAAATTAAATTACAAGAGccc                                                                                                                  >  1:1104469/1‑147 (MQ=255)
                              gTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAg                                                                                                                 <  2:3072302/148‑1 (MQ=255)
                               tACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTAGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTATTATAATTGACCATATTCCAAAATAAAGAATTGGCATGTGCCaca                                                                                                                >  2:732621/1‑146 (MQ=255)
                                 cacaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACg                                                                                                              >  2:1716873/1‑148 (MQ=255)
                                 cacaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGAAAATAATTAATTGCCATTAGCCAGACg                                                                                                              >  2:2816904/1‑148 (MQ=255)
                                     ggCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGTCTCTATGCGAAAATAATGAATTTCCATGAGCCAGACgcagc                                                                                                          >  2:1458525/1‑148 (MQ=255)
                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTCAATTACTAAATTCGCAAAAAATGAATTGCCATGAGCCACACgccgca                                                                                                         >  1:3418181/1‑148 (MQ=255)
                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACgcagca                                                                                                         >  2:170342/1‑148 (MQ=255)
                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATTCGAAAATAATGAATTTCCATGAGCCAGACgcagca                                                                                                         >  2:564756/1‑148 (MQ=255)
                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGTATCTATGCGAAAATAATTAATTTGCATGAGCCACACccagca                                                                                                         >  1:2865932/1‑148 (MQ=255)
                                      gCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATTACTCTATTCGAAAAAAATTAATTTCCATGAGCCACTAgcagca                                                                                                         >  2:32070/1‑148 (MQ=255)
                                        tGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGcaca                                                                                                       <  1:3081400/148‑1 (MQ=255)
                                        tGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGcaca                                                                                                       <  1:2704700/148‑1 (MQ=255)
                                         ggATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGTCTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAt                                                                                                      >  2:2364517/1‑148 (MQ=255)
                                          gATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAtt                                                                                                     >  2:2143265/1‑148 (MQ=255)
                                           aTTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAAAGTATTGCCATGAGCCCGACGCAGcacaata                                                                                                    >  2:2848602/1‑145 (MQ=255)
                                            tttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCt                                                                                                   <  1:137723/148‑1 (MQ=255)
                                                   ccTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTAAGCTATGCGAAAAAAATGAAATTCCAATAGCCCGACGCACCACAATATTGCAATc                                                                                            >  1:2669846/1‑148 (MQ=255)
                                                    cTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCg                                                                                           <  2:46563/148‑1 (MQ=255)
                                                       ccATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACg                                                                                        <  1:705140/148‑1 (MQ=255)
                                                         aTCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtg                                                                                      <  1:3299817/148‑1 (MQ=255)
                                                          tCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgc                                                                                     <  2:2984163/148‑1 (MQ=255)
                                                          tCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATGCGAAAATAATTAATTTCCATGAGCCAGACGCAGCACATTCTTGCATTAGACGtgc                                                                                     >  2:2270083/1‑148 (MQ=255)
                                                           cAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAATTCCATTAGCCAGACGCAGCACAATATTTCATTCGACGtgct                                                                                    >  2:2456214/1‑148 (MQ=255)
                                                           cAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTAATCTATTCGAAAATAAATAATTTCCAAGAGCCAGACGCAGCACAATATTTCATTCGAAGtgat                                                                                    >  2:670532/1‑146 (MQ=255)
                                                            aaCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgctg                                                                                   <  1:69707/148‑1 (MQ=255)
                                                            aaCTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgctg                                                                                   <  1:68656/148‑1 (MQ=255)
                                                              cTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTTCTCAATTCGAAAATAATGAATTTGCAAGAGCCAGACGCAGCACAATAGTTCATTCGACGTGCTgcg                                                                                 >  2:1244994/1‑148 (MQ=255)
                                                                 tATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCt                                                                              <  1:3350457/148‑1 (MQ=255)
                                                                 tATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATTACTCTATGCGCAAATAATGTATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCt                                                                              >  2:818106/1‑148 (MQ=255)
                                                                  aTTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTAATCGAAAAAAATTAATTGCCATGAGCCAGACGCAGCACAATATTTCATTCGACGTTCTGCGCCtt                                                                             >  2:185389/1‑148 (MQ=255)
                                                                     tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGNCTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttat                                                                          <  1:1805117/148‑1 (MQ=255)
                                                                     tGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTTCCATGAGCCCGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtttat                                                                          >  2:3522095/1‑148 (MQ=255)
                                                                       aaGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATTCGAAAATAAAGAATTGCCCTGAGCCAGACGCCGCACATTCTTGCATTCGACGTGCTGCGCCtttattt                                                                        >  2:1398159/1‑148 (MQ=255)
                                                                        aGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCGATGCGAAAATAATGAATTGCCAGGAGCCAGACGCAGCACAATCTTGCATTAGACGTGCTGCGCCgttattta                                                                       >  2:3219580/1‑148 (MQ=255)
                                                                         gCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATGATTTACTCTATTCGTAAATAAAGAATTTCCAAGAGCCAGACGCAGCACAATCTTTCATTAGACGTGCAGCGGCtttattttt                                                                      >  1:586713/1‑146 (MQ=255)
                                                                          cATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCTATGCGAAAAAAAATAATTTACATGAGCCAGACGCCGCACAGTCTTGGATTCGACGTGCTGCGCCgtttttttta                                                                     >  2:928257/1‑145 (MQ=255)
                                                                           aTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGTCGCTAAGCCAAAATAAAGAAATACCCGGAGCCCCACCCCCCACAATACTGCAATCGACCGGCGGCCCCAGTATTTATCa                                                                    >  2:2685405/1‑148 (MQ=255)
                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATGAATGACTCAATTCGAAAAAAAAGAATTTCCCAGAGCCACACGCAGCACAATCTTGCAATAGACCTGCTGCGCCTTTATTTATCAc                                                                   >  1:2005025/1‑148 (MQ=255)
                                                                            tGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCGATGCGAAAATAATGAATTTCCATGAGCCCGACGCAGCACAATCTTGCATTCGACGTGCTGCGCCGTTATTTATCAc                                                                   >  2:161818/1‑148 (MQ=255)
                                                                                  cGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAAATTACAATATTCGAAAAAAATGAAATTCCAATAGCCAGACGCAGCACATTATTTCATTAGACCTTCTGCGCCATTATTTATAACcagcag                                                             >  2:936883/1‑148 (MQ=255)
                                                                                     tGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGaa                                                          <  2:675525/148‑1 (MQ=255)
                                                                                      gATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACGCGATGCGAAAAAAAATAAATTCCAAGAGACAGACCCACCACAATATTGCATTCGACGTGCTGGGCCGTTTTTTATCACAACCACGaaa                                                         >  1:1905297/1‑148 (MQ=255)
                                                                                      gATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGaaa                                                         <  1:869635/148‑1 (MQ=255)
                                                                                         tGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGc                                                      <  1:410592/148‑1 (MQ=255)
                                                                                             aaCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTg                                                  <  1:2143265/148‑1 (MQ=255)
                                                                                              aCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGAATCTATGCGAAAATAATGTATTTCCAAGTGCCAGACGCAGCACATTCTTTCATTCGACGTGCTGCGCCTTTATTTATAACAACCAGGAAAAGCCTTGt                                                 >  2:434436/1‑148 (MQ=255)
                                                                                               ccGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTc                                                <  2:1859146/148‑1 (MQ=255)
                                                                                               ccGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTc                                                <  2:2912285/148‑1 (MQ=255)
                                                                                                   ttGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATa                                            <  1:3247897/148‑1 (MQ=255)
                                                                                                                aCTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATAACCAGCAGGAAACGCCTTGTCCATAGACGCCCCGTCca                               >  2:2268822/1‑148 (MQ=255)
                                                                                                                aCTTGATCCACACAGTCGATTTTTATAATTTAATCTATGCGAAAATAATTTAATTCCATGAGCCAGACGCAGCACAATAGTGCATTCGACGTGGTGCGCCGTTATTTATAACCAGCAGGAAACCCCATGTCCATATGCGCCCCGTCca                               >  2:392777/1‑148 (MQ=255)
                                                                                                                   tGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACAt                            <  1:140068/148‑1 (MQ=255)
                                                                                                                         acacaGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCCGCAGGAAACCCCTTTTCCATAGAAGCCCCATCCACAGGCGCca                      >  2:2077084/1‑148 (MQ=255)
                                                                                                                          cacaGTCGATTTTTATAATTTACTCTATTAGAAAAAAAAGTAATGCCATGAAACAGAACCAGAACATTATTTCATTAGACGTGCTGCGCCTTTATTTATAACCAGCAGGAAAAGCCATTTGCAAAAAAGCCCAATCAACATGCGCcac                     >  2:949710/1‑148 (MQ=255)
                                                                                                                           acaGTCGATTTTTATCAATTAATCTATGCGAAAATAATGAATTTCCATGTGCCAGACGCAGCACAATCTTTCATTAGAAGTGCTTCGCCATTATTTATAAACAACACGAAAAGCCATGTACATAGAAGCCCCATACACATTCGCccca                    >  2:437127/1‑148 (MQ=255)
                                                                                                                                cGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaaa               <  2:2588640/148‑1 (MQ=255)
                                                                                                                                 gATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAAc              <  2:2977038/148‑1 (MQ=255)
                                                                                                                                   tttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct            >  1:3389893/1‑148 (MQ=255)
                                                                                                                                   tttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct            >  2:3231372/1‑148 (MQ=255)
                                                                                                                                   tttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct            >  2:3020681/1‑148 (MQ=255)
                                                                                                                                   tttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct            <  2:473914/148‑1 (MQ=255)
                                                                                                                                   tttttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct            >  1:826598/1‑148 (MQ=255)
                                                                                                                                       tATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAt        <  1:32070/148‑1 (MQ=255)
                                                                                                                                         tCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTc      <  1:2295786/148‑1 (MQ=255)
                                                                                                                                          cATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc     >  1:491912/1‑148 (MQ=255)
                                                                                                                                          cATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc     >  1:3129699/1‑148 (MQ=255)
                                                                                                                                          cATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc     >  1:61689/1‑148 (MQ=255)
                                                                                                                                           aTTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa    >  1:1101056/1‑148 (MQ=255)
                                                                                                                                           aTTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa    >  1:919339/1‑148 (MQ=255)
                                                                                                                                           aTTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa    <  2:3292847/148‑1 (MQ=255)
                                                                                                                                           aTTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa    <  2:745885/148‑1 (MQ=255)
                                                                                                                                            ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCCTAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg   >  2:2506485/1‑148 (MQ=255)
                                                                                                                                            ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg   >  2:3343764/1‑148 (MQ=255)
                                                                                                                                            ttGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg   <  2:2387527/148‑1 (MQ=255)
                                                                                                                                             tGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGt  >  2:1027527/1‑148 (MQ=255)
                                                                                                                                             tGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGt  >  2:333320/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGT  >  CP009273/2004388‑2004676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC  >  CP009273/2004384‑2004681
                                                                                                                                                     |                                                                                                                                                    
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                      <  SRR19973067.397735/150‑1 (MQ=60)
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACT                                                                                                                                                      <  SRR19973067.1725765/150‑1 (MQ=60)
   CGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTA                                                                                                                                                   <  SRR19973067.365112/150‑1 (MQ=60)
    GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTAT                                                                                                                                                  <  SRR19973067.2694697/150‑1 (MQ=60)
     ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATG                                                                                                                                                 <  SRR19973067.1181472/150‑1 (MQ=60)
     ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATG                                                                                                                                                 <  SRR19973067.3049138/150‑1 (MQ=60)
       GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCG                                                                                                                                               <  SRR19973067.3021384/150‑1 (MQ=60)
       GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATGACTCTATTCG                                                                                                                                               >  SRR19973067.3553426/1‑150 (MQ=60)
        CCTTCATCGCCAGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGA                                                                                                                                              <  SRR19973067.3287886/150‑1 (MQ=60)
             ATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTGACTCTATTCGAAAAAA                                                                                                                                         >  SRR19973067.2557138/1‑150 (MQ=60)
              TCGCCTGATCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAA                                                                                                                                        <  SRR19973067.3392502/150‑1 (MQ=60)
              TCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAA                                                                                                                                        <  SRR19973067.2737360/150‑1 (MQ=60)
                GCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATG                                                                                                                                      <  SRR19973067.1661095/150‑1 (MQ=60)
                  CTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATGATTTAATCAATGCGAAAAAAATGAA                                                                                                                                    >  SRR19973067.1685885/1‑150 (MQ=60)
                  CTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAA                                                                                                                                    <  SRR19973067.17470/150‑1 (MQ=60)
                   TGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAT                                                                                                                                   <  SRR19973067.3369984/150‑1 (MQ=60)
                    GAGCTTAGAAACGGGTACACTGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATT                                                                                                                                  <  SRR19973067.2740458/150‑1 (MQ=60)
                         TAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCAT                                                                                                                             <  SRR19973067.3340446/150‑1 (MQ=60)
                               CGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACACTATTCGAAAAaaattaaattacaagagccc                                                                                                                       >  SRR19973067.1111974/1‑130 (MQ=60)
                                GGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAG                                                                                                                      <  SRR19973067.2499912/150‑1 (MQ=60)
                                GGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAG                                                                                                                      <  SRR19973067.706086/150‑1 (MQ=60)
                                GGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAG                                                                                                                      <  SRR19973067.3549268/150‑1 (MQ=60)
                                        AGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGTATCTATGCGAAAATAATTAATTTGCATGAGCCACACCCAGCA                                                                                                              >  SRR19973067.2884289/1‑150 (MQ=60)
                                            TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATT                                                                                                          <  SRR19973067.2722114/150‑1 (MQ=60)
                                            TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATT                                                                                                          <  SRR19973067.3101008/150‑1 (MQ=60)
                                               ATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTT                                                                                                       <  SRR19973067.1740534/150‑1 (MQ=60)
                                                TTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTG                                                                                                      <  SRR19973067.138951/150‑1 (MQ=60)
                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTAAGCTATGCGAAAAAAATGAAattccaatagcccgacgcaccacaatattgcaatc                                                                                                 >  SRR19973067.2686986/1‑115 (MQ=60)
                                                     GGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTC                                                                                                 <  SRR19973067.3110246/150‑1 (MQ=60)
                                                       CCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTCATTTACACTAAGCGAAAAAAAAGAATTGCCctgtgcccgacgccgcacaatcttggagtcgc                                                                                               >  SRR19973067.1151528/1‑118 (MQ=60)
                                                           CCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTG                                                                                           <  SRR19973067.710019/150‑1 (MQ=60)
                                                             ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCT                                                                                         <  SRR19973067.3320599/150‑1 (MQ=60)
                                                              TCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTG                                                                                        <  SRR19973067.2307789/150‑1 (MQ=60)
                                                                AACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCG                                                                                      <  SRR19973067.70522/150‑1 (MQ=60)
                                                                AACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCG                                                                                      <  SRR19973067.69465/150‑1 (MQ=60)
                                                                AACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCG                                                                                      <  SRR19973067.658239/150‑1 (MQ=60)
                                                                   TTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCT                                                                                   <  SRR19973067.47861/150‑1 (MQ=60)
                                                                     TATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACGCGATGCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTTCATTAGACGTGCTGCGCCTTT                                                                                 >  SRR19973067.571485/1‑150 (MQ=60)
                                                                     TATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTT                                                                                 <  SRR19973067.3523037/150‑1 (MQ=60)
                                                                     TATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTT                                                                                 <  SRR19973067.3371701/150‑1 (MQ=60)
                                                                      ATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTA                                                                                <  SRR19973067.3414944/150‑1 (MQ=60)
                                                                       TTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTAT                                                                               <  SRR19973067.1241284/150‑1 (MQ=60)
                                                                        TTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATT                                                                              <  SRR19973067.938535/150‑1 (MQ=60)
                                                                         TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGNCTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                             <  SRR19973067.1817278/150‑1 (MQ=60)
                                                                           AAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATGATTTACTCTATTCGTAAATAAAGAATTTCCAAGAGCCAGACGCAGCACAATCTTTCATTAGACGTGCAGCGGCTTTATTTTT                                                                           >  SRR19973067.590462/1‑150 (MQ=60)
                                                                              CATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATGAATGACTCAATTCGAAAAAAAAGAATTTCCCAGAGCCACACGCAGCACAATCTTGCAATAGACCTGCTGCGCCTTTATTTATCAC                                                                        >  SRR19973067.2018529/1‑150 (MQ=60)
                                                                                     TCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGG                                                                 <  SRR19973067.2015329/150‑1 (MQ=60)
                                                                                        CTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACGCGATGCGAAAAaaaataaattccaagagacagacccaccacaatattgcattcgacgtgctgggccgttttttatcacaaccacgaaa                                                              >  SRR19973067.1918285/1‑73 (MQ=60)
                                                                                          GATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACG                                                            <  SRR19973067.1395724/150‑1 (MQ=60)
                                                                                          GATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACG                                                            <  SRR19973067.875447/150‑1 (MQ=60)
                                                                                          GATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACG                                                            <  SRR19973067.875440/150‑1 (MQ=60)
                                                                                             TGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCT                                                         <  SRR19973067.413450/150‑1 (MQ=60)
                                                                                                 AACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTC                                                     <  SRR19973067.2157590/150‑1 (MQ=60)
                                                                                                       TTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGA                                               <  SRR19973067.3268229/150‑1 (MQ=60)
                                                                                                               TATCGACTTGATCCACACAGTCGATTTTTATAATTTTCGCTATGCGAAAATAAATAATTTCCATGAGCCACACGCAGCACAATCTTGCCTTCGACGTGGTGCGCCGTTATTTATaacccgcacgaaaccccgtgtacaaagaagccccat                                       >  SRR19973067.839846/1‑114 (MQ=60)
                                                                                                                       TGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGC                               <  SRR19973067.141477/150‑1 (MQ=60)
                                                                                                                       TGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGC                               <  SRR19973067.141309/150‑1 (MQ=60)
                                                                                                                                    CGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACC                  >  SRR19973067.3207889/1‑150 (MQ=60)
                                                                                                                                     GATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCT                 >  SRR19973067.832240/1‑150 (MQ=60)
                                                                                                                                     GATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCT                 >  SRR19973067.828729/1‑150 (MQ=60)
                                                                                                                                     GATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCT                 >  SRR19973067.3411449/1‑150 (MQ=60)
                                                                                                                                           TATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTC           <  SRR19973067.32546/150‑1 (MQ=60)
                                                                                                                                           TATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTC           <  SRR19973067.32548/150‑1 (MQ=60)
                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC          >  SRR19973067.62434/1‑150 (MQ=60)
                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC          >  SRR19973067.495295/1‑150 (MQ=60)
                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC          >  SRR19973067.2884624/1‑150 (MQ=60)
                                                                                                                                            ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC          >  SRR19973067.3149588/1‑150 (MQ=60)
                                                                                                                                             TCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA         <  SRR19973067.2310936/150‑1 (MQ=60)
                                                                                                                                             TCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA         <  SRR19973067.2311729/150‑1 (MQ=60)
                                                                                                                                             TCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA         >  SRR19973067.925489/1‑150 (MQ=60)
                                                                                                                                             TCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA         >  SRR19973067.1108546/1‑150 (MQ=60)
                                                                                                                                                    CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAACCCTCTATTCCAGTGACAC  >  SRR19973067.727119/1‑150 (MQ=60)
                                                                                                                                                    CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC  >  SRR19973067.727063/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC  >  CP009273/2004384‑2004681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑