Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,004,533 G→T 100% T151T (ACG→ACT)  yedL → putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,004,5330GT98.6% 731.4 / ‑5.0 286T151T (ACG→ACT) yedLputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (8/1);  major base T (109/154);  minor base A (14/0);  total (131/155)
Fisher's exact test for biased strand distribution p-value = 7.50e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 288 total aligned reads displayed.

CAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC  >  CP009273/2004386‑2004679
                                                                                                                                                   |                                                                                                                                                  
cAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGActct                                                                                                                                                  <  2:5054566/150‑1 (MQ=255)
 aGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                                 <  2:256014/150‑1 (MQ=255)
 aGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTa                                                                                                                                                 >  2:2959282/1‑150 (MQ=255)
 aGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTAATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTa                                                                                                                                                 <  2:4998538/150‑1 (MQ=255)
   aTGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCAATTACTCTATg                                                                                                                                               >  1:4988827/1‑150 (MQ=255)
    tGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGc                                                                                                                                              <  2:2708233/150‑1 (MQ=255)
     gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCg                                                                                                                                             <  1:1096215/150‑1 (MQ=255)
     gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCg                                                                                                                                             <  1:197907/150‑1 (MQ=255)
     gCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCg                                                                                                                                             >  2:4442556/1‑150 (MQ=255)
       cTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaa                                                                                                                                           >  2:594129/1‑150 (MQ=255)
       cTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaa                                                                                                                                           <  1:2831447/150‑1 (MQ=255)
         tCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTCATTTACTCAAAGCGaaaa                                                                                                                                         >  2:2215485/1‑150 (MQ=255)
         tCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGaaaa                                                                                                                                         <  2:4353006/150‑1 (MQ=255)
           aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAaata                                                                                                                                       >  2:3264714/1‑150 (MQ=255)
              gCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAAATTACTGTATGAGGAaataatt                                                                                                                                    >  1:1363715/1‑149 (MQ=255)
               ccTGAGGATAGAAACGGGTACACAGGCTTGATTTACGGCCTGCCATCATCTTTATTTGAAGCTTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGa                                                                                                                                   <  2:2842913/150‑1 (MQ=255)
                cTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGTAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGaa                                                                                                                                  <  1:2320111/150‑1 (MQ=255)
                 tGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAAt                                                                                                                                 <  1:4311250/150‑1 (MQ=255)
                  gAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTACTCGATGCGGAAATAATGTAtt                                                                                                                                >  1:36114/1‑150 (MQ=255)
                   aGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAACTAATGAATTg                                                                                                                               <  2:5272300/150‑1 (MQ=255)
                   aGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTg                                                                                                                               <  2:3809330/150‑1 (MQ=255)
                    gCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGc                                                                                                                              <  1:3501269/150‑1 (MQ=255)
                        aGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATg                                                                                                                          <  2:4673155/150‑1 (MQ=255)
                          aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATTACTATATTAGAAAAAAATGAATTTCCATGAg                                                                                                                        >  1:1076213/1‑150 (MQ=255)
                          aaaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCATTTGCTCGATGCGAAAAAAATTAATTGGCATGAg                                                                                                                        >  1:2930369/1‑150 (MQ=255)
                           aaCGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGc                                                                                                                       <  2:4410584/150‑1 (MQ=255)
                              gggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGTGCCAg                                                                                                                    >  2:2796226/1‑150 (MQ=255)
                              gggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATTAATATATGCGAAAAAAATGAATTTCACTGAGCCAg                                                                                                                    >  2:4080408/1‑150 (MQ=255)
                                gTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTCTATGCGAAAATAAAGAAATTCCATGAGCCAGAc                                                                                                                  >  1:4399508/1‑150 (MQ=255)
                                gTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGAc                                                                                                                  <  1:2117516/150‑1 (MQ=255)
                                   cacaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGAATCTATGCGAAAATAATTAATTGCCATGATCCAGACgca                                                                                                               >  2:2824291/1‑150 (MQ=255)
                                    acaGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTTCCCTGAGCCCGACgcag                                                                                                              >  1:2949070/1‑150 (MQ=255)
                                     caGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATTCGAAAATAATGAATTGCCATGAGCCAGACgcagc                                                                                                             >  2:3438418/1‑150 (MQ=255)
                                       ggCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAAAAATGAATTGCCATGAGCCAGACGCAGcac                                                                                                           >  2:4895407/1‑150 (MQ=255)
                                         cTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAt                                                                                                         <  2:3993531/150‑1 (MQ=255)
                                          tGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAtt                                                                                                        <  1:740657/150‑1 (MQ=255)
                                          tGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCAATTTCAAAATGCCAAAATAAAGAAATTCCATGAGCCAGACGCAGCACAtt                                                                                                        >  2:4409804/1‑150 (MQ=255)
                                             aTTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCtt                                                                                                     <  2:2626247/150‑1 (MQ=255)
                                              tttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTg                                                                                                    <  2:1183640/150‑1 (MQ=255)
                                                      cTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCAATGCGAAAATAATGAAATGCCAAGAGCCAGACGCAGCACAATCTTGCCTTCGAc                                                                                            >  2:5120670/1‑150 (MQ=255)
                                                       tGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTTATCAATTAGAAAAAAATTAATTTCCCAGAGCCAGAAGCACCCCAATATTGCaatacacc                                                                                           >  1:1750052/1‑143 (MQ=255)
                                                        gCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGt                                                                                          <  1:1573063/150‑1 (MQ=255)
                                                         ccATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAAATTACTCTATTCGAAAATAATGAATTTCCAAGAGCCAGACGCAGCACATTCTTGCATTCGACGtg                                                                                         >  2:1635698/1‑150 (MQ=255)
                                                          cATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgc                                                                                        <  1:2492271/150‑1 (MQ=255)
                                                          cATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATTCGAAAATAATGAATTGACATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgc                                                                                        >  1:5233671/1‑150 (MQ=255)
                                                           aTCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGtgct                                                                                       <  2:1032319/150‑1 (MQ=255)
                                                           aTCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAATTTACTCTATGCGAAAATAAAGAATTGCCAAGAGCCAGACGCAGCACAGTCTTGGATTAGAAGtgcg                                                                                       >  1:304954/1‑149 (MQ=255)
                                                                  ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTAATCTATGCGAAAATAATGAATTGCCCTGAGCCCGAAGCAGCACAATCTTGCATTAGACGTGCTGCGCCtt                                                                                >  2:148749/1‑150 (MQ=255)
                                                                  ttATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCtt                                                                                <  2:3686700/150‑1 (MQ=255)
                                                                    aTTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCttta                                                                              <  2:1783934/150‑1 (MQ=255)
                                                                    aTTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTACTCAATGCGAAAATAATTAATTTACAAGAGCCAGACGCAGCACATTATTTGATTCGAAGTGCTGCGCCgtta                                                                              >  2:2351467/1‑150 (MQ=255)
                                                                       agAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATCAATGACTCAAAGCGAAAATAAAGAATTGCCAAGAGCCAGACGCACCACATTCATGCATTAGACCTGCTGCCCCtttattt                                                                           >  2:99298/2‑150 (MQ=255)
                                                                         aaGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTTATAATTGTCTCGATGCGAAAATAATGAATTTCCATGTGGCAGACGCAGCCCATTCTTGCCTTGGACGTGCTGGGCCTgtatttat                                                                         >  1:4387270/1‑150 (MQ=255)
                                                                          aGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAATTTTCTATATGCGAAAATAATGAATTTCCAGGAGCCCGACGCAGCACAATATTTCATTAGACGTGCTGGGGCGTTATTTATc                                                                        >  1:3194949/1‑150 (MQ=255)
                                                                          aGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAAATGAATAATTTAGAAAAAAATGAATTTCCATGAGCCCGACGCAGCCCAATCTTTCATTAGACGTGCTGCGCCTTTATTTATc                                                                        >  1:4694749/1‑150 (MQ=255)
                                                                             aTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCCGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAcc                                                                     >  2:825778/1‑150 (MQ=255)
                                                                               ggTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCTTTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcag                                                                   <  2:655126/150‑1 (MQ=255)
                                                                                 tttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGAATCGATGCGAAAATAATGTATTGGCATGAGCCAGACGCAGCACAATCATGCATTCGACGTTCTGCGCCATTATTTTTAACcagca                                                                 >  1:4001470/1‑150 (MQ=255)
                                                                                  ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcagcag                                                                <  1:330509/150‑1 (MQ=255)
                                                                                  ttCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACcagcag                                                                <  1:492557/150‑1 (MQ=255)
                                                                                   tCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTTCTGCGCCTTTATTTATCACCAGCAgg                                                               <  2:777077/150‑1 (MQ=255)
                                                                                     gCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGaa                                                             <  2:4948121/150‑1 (MQ=255)
                                                                                      cTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATTCGAAAAAAATGAATTTCCATTATCCAGACGCAGCACAATAGTTCATTCGACGTGCTGCGCCTTTATTTATAAACAGCAGGaaa                                                            >  2:1539418/1‑150 (MQ=255)
                                                                                      cTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGaaa                                                            <  1:854290/150‑1 (MQ=255)
                                                                                        gATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACg                                                          <  1:1255848/150‑1 (MQ=255)
                                                                                        gATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTTAAACTATTCGAAAAAAATTAATTTCCAAGAGCCCGACGCAGCACATTATTGCAATAGACGTGGTGCGCCGTTATTTATAACCCGCAGGAAACg                                                          >  1:769578/1‑150 (MQ=255)
                                                                                        gATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATAATTGACTCTATGCGGAAATAATGAATTTCCATGTGCCAGACGCAGCACAATATTTCATTAGACGTGCTGCGCCATTATTTATAACAAGCAGGAAACg                                                          >  2:4217557/1‑150 (MQ=255)
                                                                                            gCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCtt                                                      <  1:466408/150‑1 (MQ=255)
                                                                                            gCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGACAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCtt                                                      <  2:137938/150‑1 (MQ=255)
                                                                                                    tttGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAg                                              <  2:4103775/150‑1 (MQ=255)
                                                                                                        cTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTTACTATATGCGAAAATAATTAATTTCCATGAGCCAGACGCAGCACAATCATTCATTCGACGTGCTGGGGCTTTATTTATAACCAGCAGGAAACGCCATGTACATATACGc                                          >  2:3145945/1‑150 (MQ=255)
                                                                                                           aTTATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGcccc                                       <  2:3507371/150‑1 (MQ=255)
                                                                                                            ttATCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCt                                      <  1:2421709/150‑1 (MQ=255)
                                                                                                              aTCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATTAATTTCCATGAGCCAGACGCAGCACAATCTTGCATTAGACGTGCTGCGCCTTTATTTATCAACAGCAGGAAACGCCGTGTCCATAGACGCCCCATc                                    >  2:2101482/1‑150 (MQ=255)
                                                                                                               tCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTcc                                   <  1:4102457/150‑1 (MQ=255)
                                                                                                               tCGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTcc                                   <  1:1452108/150‑1 (MQ=255)
                                                                                                               tCGACTTGATCCACACAGTCGATTTTTATAATTTTCTCAATTCGAAAATAAATAATTTGCATTAGCCAGACGCAGCACAATCTTGCATTAGACGTGCTGCGCCATTTTTTATAACCACCCGGAAACGCCGTGTCCATAGACGCCCCATcc                                   >  1:777816/1‑150 (MQ=255)
                                                                                                                cGACTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCca                                  <  2:2004950/150‑1 (MQ=255)
                                                                                                                  aCTTGATCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCcaca                                <  1:4859082/150‑1 (MQ=255)
                                                                                                                  aCTTGATCCACACAGTCGATTTTTATCATTGACTATATTCGAAAAAAATGAAATTCCATGAGCCAGAAGCAGCACAATATTTAATTCGACGTGCTGCGCCATTATTTATAAACAGCAGGAAACGCCTTGTACAAAGACGCCCCATAcaca                                >  2:2138885/1‑150 (MQ=255)
                                                                                                                       aTCCACACAGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATgcgc                           <  1:5182399/150‑1 (MQ=255)
                                                                                                                         ccACACAGTCGATTTTTATCATTGTCTCTATTCGAAAAAAAATAATTTCCAATAGCCAGAAGCAGCACATTATTTCATTCGACGTGCTGCGCCTTTATTTATCAACCGCAGGAAACGCCTTGTCCATAGTCGCCCCTTCCACAAGCGCca                         >  2:2751193/1‑150 (MQ=255)
                                                                                                                             acaGTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAg                     <  1:3743881/150‑1 (MQ=255)
                                                                                                                                gTCGATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGaaa                  >  2:1198016/1‑150 (MQ=255)
                                                                                                                                   gATTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACct               >  2:1883237/1‑150 (MQ=255)
                                                                                                                                    aTTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCTACAAGAAACctc              <  2:2469045/150‑1 (MQ=255)
                                                                                                                                    aTTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctc              >  1:3562468/1‑150 (MQ=255)
                                                                                                                                    aTTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctc              >  1:2984934/1‑150 (MQ=255)
                                                                                                                                    aTTTTTATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctc              >  1:1179013/1‑150 (MQ=255)
                                                                                                                                       tttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAt           <  1:3264714/150‑1 (MQ=255)
                                                                                                                                       tttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAt           >  2:4288951/1‑150 (MQ=255)
                                                                                                                                        ttATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATATACGCCCCTTCCACATGCGCCACAAGAAACCTCTAtt          >  1:4733847/1‑150 (MQ=255)
                                                                                                                                          aTCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc        <  1:2580714/150‑1 (MQ=255)
                                                                                                                                          aTCATTGACTCTATGCGAAAATAATGAATTGCCATGAGACAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTcc        >  2:4739586/1‑150 (MQ=255)
                                                                                                                                           tCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa       <  2:3922101/150‑1 (MQ=255)
                                                                                                                                           tCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCa       <  1:549934/150‑1 (MQ=255)
                                                                                                                                             aTTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACACGAAACCTCTATTCCAGt     >  1:3800775/1‑150 (MQ=255)
                                                                                                                                             aTTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGACTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGt     <  2:2182826/150‑1 (MQ=255)
                                                                                                                                                gACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGac  >  1:20161/1‑150 (MQ=255)
                                                                                                                                                gACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGac  <  2:681205/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC  >  CP009273/2004386‑2004679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACA  >  CP009273/2004388‑2004682
                                                                                                                                                         |                                                                                                                                                     
GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTAT                                                                                                                                                   <  SRR19973062.2860445/150‑1 (MQ=60)
 ATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCAATTACTCTATG                                                                                                                                                  >  SRR19973062.5015180/1‑150 (MQ=60)
   GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCG                                                                                                                                                <  SRR19973062.1101373/150‑1 (MQ=60)
   GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCG                                                                                                                                                <  SRR19973062.4913851/150‑1 (MQ=60)
   GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCG                                                                                                                                                <  SRR19973062.1101284/150‑1 (MQ=60)
   GCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCG                                                                                                                                                <  SRR19973062.198975/150‑1 (MQ=60)
     CTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAA                                                                                                                                              <  SRR19973062.2846425/150‑1 (MQ=60)
     CTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGGTTT‑‑‑‑‑‑‑‑ATCAGTGACGCAATGCGAA                                                                                                                                              >  SRR19973062.4771809/1‑150 (MQ=60)
        CATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAAT                                                                                                                                           <  SRR19973062.300259/150‑1 (MQ=60)
        CATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAAT                                                                                                                                           <  SRR19973062.2997862/150‑1 (MQ=60)
          TCGCCTGAGCTTAGAAACGGTTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTATATGCGAAAATAA                                                                                                                                         <  SRR19973062.4376088/150‑1 (MQ=60)
              CTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGTAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAA                                                                                                                                     <  SRR19973062.2332349/150‑1 (MQ=60)
              CTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAA                                                                                                                                     <  SRR19973062.2332360/150‑1 (MQ=60)
              CTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACGCTATGCGAAAATAATGAA                                                                                                                                     <  SRR19973062.3319215/150‑1 (MQ=60)
               TGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAAT                                                                                                                                    <  SRR19973062.820967/150‑1 (MQ=60)
               TGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAAT                                                                                                                                    <  SRR19973062.4333486/150‑1 (MQ=60)
                GAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAAATGAAGCAATGCGAAAAAAATGAAAT                                                                                                                                   >  SRR19973062.4365593/1‑150 (MQ=60)
                  GCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGC                                                                                                                                 <  SRR19973062.3519035/150‑1 (MQ=60)
                    TTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCA                                                                                                                               <  SRR19973062.2956030/150‑1 (MQ=60)
                    TTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCA                                                                                                                               <  SRR19973062.2717329/150‑1 (MQ=60)
                     TAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCAT                                                                                                                              <  SRR19973062.4697442/150‑1 (MQ=60)
                      AGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGAATCTATGCGAAAATAATTAATTTCCATG                                                                                                                             >  SRR19973062.2980735/1‑150 (MQ=60)
                        AAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTT‑‑‑‑‑‑‑‑ATCATTTGCTCGATGCGAAAAAAATTAATTGGCATGAG                                                                                                                           >  SRR19973062.2945795/1‑150 (MQ=60)
                        AAACGGGTACACAGGATGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAG                                                                                                                           <  SRR19973062.3762461/150‑1 (MQ=60)
                         AACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGC                                                                                                                          <  SRR19973062.4155046/150‑1 (MQ=60)
                           CGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCA                                                                                                                        <  SRR19973062.381191/150‑1 (MQ=60)
                           CGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCA                                                                                                                        <  SRR19973062.3451084/150‑1 (MQ=60)
                              GTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTTACTCTATGCGAAAATAAAGAAATTCCATGAGCCAGAC                                                                                                                     >  SRR19973062.4422026/1‑150 (MQ=60)
                              GTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGAC                                                                                                                     <  SRR19973062.3888512/150‑1 (MQ=60)
                              GTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGAC                                                                                                                     <  SRR19973062.2601655/150‑1 (MQ=60)
                              GTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGAC                                                                                                                     <  SRR19973062.2128292/150‑1 (MQ=60)
                               TACACTGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACG                                                                                                                    <  SRR19973062.4600148/150‑1 (MQ=60)
                                  ACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTTCCCTGAGCCCGACGCAG                                                                                                                 >  SRR19973062.2964570/1‑150 (MQ=60)
                                    AGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTGACTATAAGGGAAAATAATGAATTGGCATGAGCCCGAAGCAGCA                                                                                                               >  SRR19973062.5296143/1‑150 (MQ=60)
                                     GGCTGGATTTACGTCCTGCCATCAACTTTATTTGTAGCATGGTTTCGCTTATTGCGATCCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTATATGCGAAAATAATGAATTGCCTTGAGCCAGACGCAGCAC                                                                                                              <  SRR19973062.1161115/150‑1 (MQ=60)
                                       CTGGTTTTACGGCCTGCCATCAACTTTATTTGAAGCTTGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAT                                                                                                            <  SRR19973062.4036877/150‑1 (MQ=60)
                                       CTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAT                                                                                                            <  SRR19973062.1703201/150‑1 (MQ=60)
                                       CTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACAT                                                                                                            <  SRR19973062.4036889/150‑1 (MQ=60)
                                        TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTTACTCTATTAGAAAATTATTAATTGGCATGAGCCAGACGCAGCACAAT                                                                                                           >  SRR19973062.780976/1‑150 (MQ=60)
                                        TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATT                                                                                                           <  SRR19973062.744019/150‑1 (MQ=60)
                                        TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTGACTCGATGCGAAAATAAAGAATTTCCATGAGCCAGACGCAGCACAAT                                                                                                           >  SRR19973062.3464539/1‑150 (MQ=60)
                                        TGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTT‑‑‑‑‑‑‑‑ATAATTTACTCTATTCGAAAATAAAGAATTGCCAGGAGCCCCACGCAGCACAAT                                                                                                           >  SRR19973062.780980/1‑150 (MQ=60)
                                          GATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCT                                                                                                         <  SRR19973062.1109315/150‑1 (MQ=60)
                                          GATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAAATTAATCTAATCGAAAATAATGAATTTGCAAGATCCAGACGCAGCACATTCT                                                                                                         >  SRR19973062.3705874/1‑150 (MQ=60)
                                              TACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCA                                                                                                     <  SRR19973062.2250831/150‑1 (MQ=60)
                                                    CTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGAC                                                                                               <  SRR19973062.4975881/150‑1 (MQ=60)
                                                      GCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGT                                                                                             <  SRR19973062.1580842/150‑1 (MQ=60)
                                                        CATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGC                                                                                           <  SRR19973062.2505605/150‑1 (MQ=60)
                                                        CATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCAGTTTCAATATGCGAAAATAAAGTATTTCCATGAGCCAGACGCAGCACAATCTTGGATTCGACGTGC                                                                                           >  SRR19973062.1739824/1‑150 (MQ=60)
                                                        CATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTGACTCTATTCGAAAATAATGAATTGACATGAGCCAGACGCAGCACAATCTTGCATTAGACGTGC                                                                                           >  SRR19973062.5261245/1‑150 (MQ=60)
                                                         ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTTATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCT                                                                                          <  SRR19973062.5009762/150‑1 (MQ=60)
                                                         ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTTACACAATGCGAAAAAAATTAATTTCCAGTATCCAGACGCAGCACAATCATGCATTCGTCGTGGT                                                                                          >  SRR19973062.306407/1‑150 (MQ=60)
                                                         ATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTT‑‑‑‑‑‑‑‑ATAATTTACTCTATGCGAAAATAAAGAATTGCCAAGAGCCAGACGCAGCACAGTCTTGgattagaagtgcg                                                                                          >  SRR19973062.306408/1‑137 (MQ=60)
                                                          TCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTG                                                                                         <  SRR19973062.3220496/150‑1 (MQ=60)
                                                             ACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTGACAATATTCGAAAATAATGAATTTCCAAGTGCCAGACCCAGCACAATATTTCATTAGACGTGCGGCGC                                                                                      >  SRR19973062.3285514/1‑150 (MQ=60)
                                                               TTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCT                                                                                    <  SRR19973062.2475292/150‑1 (MQ=60)
                                                               TTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCT                                                                                    <  SRR19973062.5284640/150‑1 (MQ=60)
                                                                    TTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATT                                                                               <  SRR19973062.4945946/150‑1 (MQ=60)
                                                                    TTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATT                                                                               <  SRR19973062.3571058/150‑1 (MQ=60)
                                                                     TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                              <  SRR19973062.1773325/150‑1 (MQ=60)
                                                                     TGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTT                                                                              <  SRR19973062.4744232/150‑1 (MQ=60)
                                                                       AAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGAGTTTT‑‑‑‑‑‑‑‑ATAATTGTCTCGATGCGAAAATAATGAATTTCCATGTGGCAGACGCAGCCCATTCTTGCCTTGGACGTGCTGGGCCTGTATTTAT                                                                            >  SRR19973062.4409750/1‑150 (MQ=60)
                                                                        AGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTTTAAATGAATAATTTA‑‑‑‑‑‑‑‑GAAAAAAATGAATTTCCATGAGCCCGACGCAGCCCAATCTTTCATTAGACGTGCTGCGCCTTTATTTATC                                                                           >  SRR19973062.4719277/1‑150 (MQ=60)
                                                                        AGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑TTAATTTTCTATATGCGAAAATAATGAATTTCCAGGAGCCCGACGCAGCACAATATTTCATTAGACGTGCTGGGGCGTTATTTATC                                                                           >  SRR19973062.3211538/1‑150 (MQ=60)
                                                                          CATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAC                                                                         <  SRR19973062.5132516/150‑1 (MQ=60)
                                                                           ATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCAATGAATATATTCGAAAATAATGAATTTCCATGAGCCAGACGCAGCACATTCTTTCATTCGACGTGCTGCGGCTTTATTTATAACC                                                                        >  SRR19973062.3965640/1‑150 (MQ=60)
                                                                           ATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTTTCTCTATTCGAAAAAAATTAATTTGCCTGATCCAGACGCAGCACAATATTTCATTCGACGTGCTGCGCCTTTATTTATaaac                                                                        >  SRR19973062.4414312/1‑146 (MQ=60)
                                                                            TGGTTTCGCTGATTTCGAACCGTTTGCTGATTATCGTCTTGATCCACACAGTCGTTTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCTAAAATAATGAATTGCCTTGAGCCAGACGCAGCACTTTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCA                                                                       <  SRR19973062.3620425/150‑1 (MQ=60)
                                                                            TGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCA                                                                       <  SRR19973062.3620353/150‑1 (MQ=60)
                                                                             GGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAG                                                                      <  SRR19973062.2102651/150‑1 (MQ=60)
                                                                               TTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTGAATCGATGCGAAAATAATGTATTGGCATGAGCCAGACGCAGCACAATCATGCATTCGACGTTCTGCGCCATTATTTTTAACCAGCA                                                                    >  SRR19973062.4021678/1‑150 (MQ=60)
                                                                                TTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAG                                                                   <  SRR19973062.332042/150‑1 (MQ=60)
                                                                                TTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAG                                                                   <  SRR19973062.494838/150‑1 (MQ=60)
                                                                                    CTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAA                                                               <  SRR19973062.857977/150‑1 (MQ=60)
                                                                                    CTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTGACTCTATTCGAAAATAATGAAATTGCAAGAGCCAGACGCAACACAATAATGCATTAGAAGTGCTGCGCCATTATTTATAAACAGCAGGAAA                                                               >  SRR19973062.2561029/1‑150 (MQ=60)
                                                                                      GATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACG                                                             <  SRR19973062.1261646/150‑1 (MQ=60)
                                                                                      GATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTTAAACTATTCGAAAAAAATTAATTTCCAAGAGCCCGACGCAGCACATTATTGCAATAGACGTGGTGCGCCGTTATTTATAACCCGCAGGAAACG                                                             >  SRR19973062.773038/1‑150 (MQ=60)
                                                                                          GCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTT                                                         <  SRR19973062.468480/150‑1 (MQ=60)
                                                                                                  TTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAG                                                 <  SRR19973062.751352/150‑1 (MQ=60)
                                                                                                   TTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTTACTCTATTCGAAAATAATTAATTTGCATGAGCCAGACGCAGCACAATATTTCATTCGACGTGCTGCGCCTTTATTTATAACCAGCAGGAAACGCCTTGTACAAAGA                                                >  SRR19973062.3073593/1‑150 (MQ=60)
                                                                                                    TGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                               <  SRR19973062.3206733/150‑1 (MQ=60)
                                                                                                    TGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                               <  SRR19973062.149614/150‑1 (MQ=60)
                                                                                                    TGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                               <  SRR19973062.1203509/150‑1 (MQ=60)
                                                                                                    TGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGAC                                               <  SRR19973062.1203432/150‑1 (MQ=60)
                                                                                                         ATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAAATTACTCTATTCGAAAATAATGAATTTGCATGAGCCAGACGCAGCACAATATTTCATTAGACGTGCTGCGCCGTTATTTATAACCAGCAGGAAAACCCATGTCCATATACGCCCC                                          >  SRR19973062.1778461/1‑150 (MQ=60)
                                                                                                          TTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCT                                         <  SRR19973062.323034/150‑1 (MQ=60)
                                                                                                          TTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCT                                         <  SRR19973062.2434718/150‑1 (MQ=60)
                                                                                                           TATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTT                                        <  SRR19973062.285296/150‑1 (MQ=60)
                                                                                                             TCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCC                                      <  SRR19973062.1459191/150‑1 (MQ=60)
                                                                                                             TCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCC                                      <  SRR19973062.4123623/150‑1 (MQ=60)
                                                                                                             TCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATAATTTTCTCAATTCGAAAATAAATAATTTGCATTAGCCAGACGCAGCACAATCTTGCATTAGACGTGCTGCGCCATTTTTTATAACCACCCGGAAACGCCGTGTCCATAGACGCCCCATCC                                      >  SRR19973062.781294/1‑150 (MQ=39)
                                                                                                             TCGACTTGATCCACACAGTCGAGTTTT‑‑‑‑‑‑‑‑TTAATTGACTCTATGCGAAAATAATGAATTGGCATTAGCCCGACGCCGCACAATATTTCATTCGACGTGCTGCGCCGTTATTTTTAACCAGCAGGAAACcccatgtacatatacgccccgacc                                      >  SRR19973062.781371/1‑126 (MQ=54)
                                                                                                              CGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑TTAATTTTCTCTATTCGAAAATAAATAATTTACAAGAGCCAGACGCAGCACAATATTTGAATCGACGTGCTGCGCCGTTATTTATAAACAGCAGGAAACCCCGTTTCCAAAGACGCCCCATCCA                                     >  SRR19973062.1187253/1‑150 (MQ=12)
                                                                                                              CGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCA                                     <  SRR19973062.5216124/150‑1 (MQ=60)
                                                                                                              CGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCA                                     <  SRR19973062.4165368/150‑1 (MQ=60)
                                                                                                              CGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCA                                     <  SRR19973062.1998222/150‑1 (MQ=60)
                                                                                                                ACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACA                                   <  SRR19973062.4884148/150‑1 (MQ=60)
                                                                                                                     ATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGC                              <  SRR19973062.5209758/150‑1 (MQ=60)
                                                                                                                           ACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAG                        <  SRR19973062.908528/150‑1 (MQ=60)
                                                                                                                           ACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAG                        <  SRR19973062.3763086/150‑1 (MQ=60)
                                                                                                                            CAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCATCCACATGCGCCACAAGA                       >  SRR19973062.4415359/1‑150 (MQ=60)
                                                                                                                                 GATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCT                  <  SRR19973062.219967/150‑1 (MQ=60)
                                                                                                                                  ATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTC                 >  SRR19973062.3580431/1‑150 (MQ=60)
                                                                                                                                  ATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTC                 >  SRR19973062.3540237/1‑150 (MQ=60)
                                                                                                                                  ATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTC                 >  SRR19973062.3000585/1‑150 (MQ=60)
                                                                                                                                  ATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTC                 <  SRR19973062.1374935/150‑1 (MQ=60)
                                                                                                                                  ATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTC                 >  SRR19973062.2464567/1‑150 (MQ=60)
                                                                                                                                  ATTTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTC                 >  SRR19973062.1184529/1‑150 (MQ=60)
                                                                                                                                    TTTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTA               <  SRR19973062.2091672/150‑1 (MQ=60)
                                                                                                                                     TTT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTAT              <  SRR19973062.3281646/150‑1 (MQ=60)
                                                                                                                                      TT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATATACGCCCCTTCCACATGCGCCACAAGAAACCTCTATT             >  SRR19973062.4758530/1‑150 (MQ=60)
                                                                                                                                      TT‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATT             >  SRR19973062.640562/1‑150 (MQ=60)
                                                                                                                                       T‑‑‑‑‑‑‑‑ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTC            <  SRR19973062.1436619/150‑1 (MQ=60)
                                                                                                                                                ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC           <  SRR19973062.2594415/150‑1 (MQ=60)
                                                                                                                                                ATCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCC           <  SRR19973062.2593978/150‑1 (MQ=60)
                                                                                                                                                 TCATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCA          <  SRR19973062.552529/150‑1 (MQ=60)
                                                                                                                                                   ATTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACACGAAACCTCTATTCCAGT        >  SRR19973062.3820228/1‑150 (MQ=60)
                                                                                                                                                    TTGACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTG       <  SRR19973062.1795546/150‑1 (MQ=60)
                                                                                                                                                      GACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGAC     >  SRR19973062.20334/1‑150 (MQ=60)
                                                                                                                                                       ACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACA    <  SRR19973062.1308037/150‑1 (MQ=60)
                                                                                                                                                       ACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACA    <  SRR19973062.2456273/150‑1 (MQ=60)
                                                                                                                                                       ACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACA    <  SRR19973062.1307994/150‑1 (MQ=60)
                                                                                                                                                       ACTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACA    >  SRR19973062.2888513/1‑150 (MQ=60)
                                                                                                                                                        CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC   >  SRR19973062.3298806/1‑150 (MQ=60)
                                                                                                                                                        CTCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACAC   >  SRR19973062.1860681/1‑150 (MQ=60)
                                                                                                                                                         TCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACA  <  SRR19973062.88379/150‑1 (MQ=60)
                                                                                                                                                         |                                                                                                                                                     
GATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTT‑‑‑‑‑‑‑‑ATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACA  >  CP009273/2004388‑2004682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑