Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 616.2 / ‑6.7 189intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (83/105);  minor base T (0/1);  total (83/106)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 189 total aligned reads displayed.

ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGT  >  CP009273/450145‑450429
                                                                                                                                                 |                                                                                                                                             
aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt                                                                                                                                             >  2:2247921/1‑148 (MQ=255)
 tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                            >  1:2235281/1‑148 (MQ=255)
 tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                            <  2:3424741/148‑1 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                          >  2:1588112/1‑148 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                          <  1:3436220/148‑1 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                          <  1:3518989/148‑1 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                          <  2:642390/148‑1 (MQ=255)
       ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                      <  2:3225008/148‑1 (MQ=255)
       ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                      <  1:1567074/148‑1 (MQ=255)
       ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                      <  1:1568014/148‑1 (MQ=255)
         ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaa                                                                                                                                    <  1:889365/148‑1 (MQ=255)
          tctGCATACTTACACTATTCAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                   <  2:2199512/148‑1 (MQ=255)
          tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                   <  2:3105175/148‑1 (MQ=255)
           ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                  >  2:1731326/1‑148 (MQ=255)
             gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                >  1:3214752/1‑148 (MQ=255)
             gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                >  1:3073880/1‑148 (MQ=255)
             gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATTAAAAAcc                                                                                                                                >  1:3336254/1‑147 (MQ=255)
              cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg                                                                                                                               >  2:1118281/1‑148 (MQ=255)
              cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg                                                                                                                               <  2:290493/148‑1 (MQ=255)
               aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                              >  2:134566/1‑148 (MQ=255)
                tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                             <  1:2589212/148‑1 (MQ=255)
                tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                             >  2:1222287/1‑148 (MQ=255)
                tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                             >  2:100981/1‑148 (MQ=255)
                tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                             >  2:1221650/1‑148 (MQ=255)
                 aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                            >  1:2921202/1‑148 (MQ=255)
                 aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                            >  2:1757815/1‑148 (MQ=255)
                    tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                         >  2:716801/1‑148 (MQ=255)
                          aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt                                                                                                                   <  1:3244541/148‑1 (MQ=255)
                           ttATGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                  <  1:383290/148‑1 (MQ=255)
                            tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTAGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                 >  2:1005315/1‑148 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                              >  2:1804914/1‑148 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                              <  1:448523/148‑1 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                              >  2:3352900/1‑148 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgggttgt                                                                                                              >  2:1805623/1‑148 (MQ=255)
                                gtgTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgtt                                                                                                             <  1:158331/146‑1 (MQ=255)
                                  gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgtttt                                                                                                           <  2:3453995/148‑1 (MQ=255)
                                     ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGttgtttttgta                                                                                                        >  2:1509169/1‑147 (MQ=255)
                                       aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttgttttttatg                                                                                                      >  2:2835362/1‑143 (MQ=255)
                                         gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGa                                                                                                    <  1:3232437/148‑1 (MQ=255)
                                         gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGa                                                                                                    <  1:1716920/148‑1 (MQ=255)
                                          ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGAt                                                                                                   >  2:2559567/1‑148 (MQ=255)
                                          ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt                                                                                                   <  2:2501876/148‑1 (MQ=255)
                                           gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtt                                                                                                  <  2:2756924/148‑1 (MQ=255)
                                              gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                               <  2:910389/148‑1 (MQ=255)
                                                   acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                          <  1:1093829/148‑1 (MQ=255)
                                                   acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCtt                                                                                          >  2:1711169/1‑148 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                         <  1:1276068/148‑1 (MQ=255)
                                                     acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                                        <  1:539922/148‑1 (MQ=255)
                                                        gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAAGGCCTGCGGGCCGTGTTGTTTTTTGTTGATTTTTGGCgtttttc                                                                                     >  2:613120/1‑146 (MQ=255)
                                                         tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                    <  1:258875/148‑1 (MQ=255)
                                                         tCTTGGCCTCACCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGcc                                                                                    <  1:2103425/148‑1 (MQ=255)
                                                          cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTTTGTGGTTTTTTGGCttttttgcc                                                                                   >  1:2555137/1‑144 (MQ=255)
                                                            ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGTCCAg                                                                                 <  2:1451374/148‑1 (MQ=255)
                                                            ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTGTGTGGGTTTTGGGGttttttggccg                                                                                 >  1:2140678/1‑142 (MQ=255)
                                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                             <  1:1384234/148‑1 (MQ=255)
                                                                 tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTGGTGGATTTTTGGCTTTTTTGCCAGCATTc                                                                            >  1:2373171/1‑148 (MQ=255)
                                                                  ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                           <  2:1688556/148‑1 (MQ=255)
                                                                  ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                           >  2:778755/1‑148 (MQ=255)
                                                                   tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                          <  1:1184742/148‑1 (MQ=255)
                                                                     ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGTGTTTGTGGTATTTTACCAGCAAACAGaa                                                                        >  2:3339820/1‑147 (MQ=255)
                                                                        ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAATCAGACG‑aa                                                                    >  2:1703144/1‑148 (MQ=255)
                                                                          tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGCTTTTGGGCTTTTTGCCCAGCATTCAGACG‑aaaa                                                                  >  2:2802244/1‑148 (MQ=255)
                                                                           gTCGTGGAGCAGGAAGCATCGCGTAAAATCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCCGACG‑ACAAt                                                                 >  2:3176921/1‑148 (MQ=255)
                                                                             cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTg                                                               <  1:1994569/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                              <  1:1070869/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTGTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                              <  1:2426854/148‑1 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGTGTTTTGGCGTTTTGCCCAGCAATCAGACG‑AAAAATGG‑CCg                                                          >  1:1786666/1‑148 (MQ=255)
                                                                                      ggAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAAt                                                     <  1:1031426/148‑1 (MQ=255)
                                                                                       gAAGCATCGCGTAAAAACACATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGGTTTGTGTTGGTTTTGGGGTTTTTGGCCAGCAATCAGACGAAAAATTGC‑CCggggtt                                                     >  1:1309465/1‑145 (MQ=255)
                                                                                        aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATtg                                                   >  2:298503/1‑148 (MQ=255)
                                                                                          gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTGGTTTTTTGGGTTTTTTCCCAGCATTAAGAAG‑AAAATTTG‑CCGGGGATtggg                                                 >  1:571072/1‑146 (MQ=255)
                                                                                          gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGGTGTTTTTTCTGGGTTTTTGGCTTTTTTCCCCGCATTCAGACG‑AAAATTGCGCCgggcttgtt                                                  >  2:76700/1‑142 (MQ=255)
                                                                                            aTCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGaa                                               <  1:1917921/148‑1 (MQ=255)
                                                                                                cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGGGCTTTTTGCCCCGCCTTCAGACG‑AAAATTTC‑CCGGGAATTGTGtaaaaa                                           >  2:2476378/1‑148 (MQ=255)
                                                                                                 gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAAt                                          <  2:2743944/148‑1 (MQ=255)
                                                                                                 gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAAt                                          <  1:1558832/148‑1 (MQ=255)
                                                                                                  tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATa                                         >  2:2344736/1‑148 (MQ=255)
                                                                                                   aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGGTGGGTTGTGTGGGTTTTTGGGTTTTTGGCCACGCTACAGGAG‑CAAAATGC‑CCCGGGAATGTGTAAAAATAc                                        >  1:1968409/1‑148 (MQ=255)
                                                                                                    aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATAcg                                       <  2:3550241/148‑1 (MQ=255)
                                                                                                    aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGGTTTTTGGCTTTTTTCCCAGCATTAAGAAG‑AAAAATTG‑CCGGGTATTGTGTAAAAATaag                                       >  1:1189879/1‑146 (MQ=255)
                                                                                                       aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGTTTTTTCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATAAGCGa                                    >  2:2616789/1‑148 (MQ=255)
                                                                                                                cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                           <  2:3039354/148‑1 (MQ=255)
                                                                                                                  aCTGTCGGCGCTTTTCCATTATGTTGCAAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                         <  1:2532591/148‑1 (MQ=255)
                                                                                                                   cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                        <  1:2619495/148‑1 (MQ=255)
                                                                                                                   cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                        <  2:533197/148‑1 (MQ=255)
                                                                                                                    tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                       <  2:2235281/148‑1 (MQ=255)
                                                                                                                     gTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTGAGCTTTTTTCCCAGCAATAACACG‑AAAAATTC‑CCGGGAAATGTGAAAAAATAACCGCCAgcgcgccaaaaa                      >  2:2413259/1‑142 (MQ=255)
                                                                                                                      tCGGCTGTTTTCATGTATTTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGAATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc                     <  1:371168/148‑1 (MQ=255)
                                                                                                                      tCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGTTTTTGGGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc                     >  2:2071452/1‑148 (MQ=255)
                                                                                                                       cGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTAAGACG‑AAAATTTC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAccc                    >  2:2882221/1‑147 (MQ=255)
                                                                                                                         gcgaTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                  <  1:1684027/148‑1 (MQ=255)
                                                                                                                                 ccAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTc          <  2:131018/148‑1 (MQ=255)
                                                                                                                                  cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa         <  2:2921202/148‑1 (MQ=255)
                                                                                                                                     tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTGGGCTTTTTTGCCACCCATCAGACC‑AAAAAATA‑CCGGGAATTGTGAAAAAATAACCGACACCGCGCCAAAAACCATCTCGACTCaaaa      >  1:2284194/1‑148 (MQ=255)
                                                                                                                                     tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa      <  1:822890/148‑1 (MQ=255)
                                                                                                                                      aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa     <  2:1699912/148‑1 (MQ=255)
                                                                                                                                       tGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa    <  1:1773697/148‑1 (MQ=255)
                                                                                                                                        gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg   <  2:193048/148‑1 (MQ=255)
                                                                                                                                         tttcTAAAATTTTTTTGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt  <  1:3353584/145‑1 (MQ=255)
                                                                                                                                         ttGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCTTTTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt  <  1:3352900/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC‑CCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGT  >  CP009273/450145‑450429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCT  >  CP009273/450141‑450438
                                                                                                                                                     |                                                                                                                                                     
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                       <  SRR19973067.3362061/150‑1 (MQ=60)
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                       >  SRR19973067.3323985/1‑150 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                    <  SRR19973067.721597/150‑1 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                    >  SRR19973067.2250151/1‑150 (MQ=60)
    ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTT                                                                                                                                                   <  SRR19973067.379205/150‑1 (MQ=60)
       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                                <  SRR19973067.3541254/150‑1 (MQ=60)
       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                                <  SRR19973067.3458099/150‑1 (MQ=60)
           GGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAA                                                                                                                                            <  SRR19973067.1578736/150‑1 (MQ=60)
           GGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAA                                                                                                                                            <  SRR19973067.1577794/150‑1 (MQ=60)
           GGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAA                                                                                                                                            <  SRR19973067.1577777/150‑1 (MQ=60)
             CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                          <  SRR19973067.895259/150‑1 (MQ=60)
             CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                          >  SRR19973067.2585878/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                        >  SRR19973067.3093460/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                        >  SRR19973067.3234985/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                        >  SRR19973067.1587446/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                        >  SRR19973067.3093450/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATTAAAAACC                                                                                                                                        >  SRR19973067.3357377/1‑150 (MQ=60)
                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                    >  SRR19973067.2939777/1‑150 (MQ=60)
                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                    <  SRR19973067.536914/150‑1 (MQ=60)
                    TACTTACACTATTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTG                                                                                                                                   <  SRR19973067.2606785/150‑1 (MQ=60)
                    TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTG                                                                                                                                   <  SRR19973067.2606066/150‑1 (MQ=60)
                     ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                                  >  SRR19973067.2412427/1‑150 (MQ=60)
                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                         <  SRR19973067.3264865/150‑1 (MQ=60)
                               TTATGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTT                                                                                                                        <  SRR19973067.385960/150‑1 (MQ=60)
                                  AGGAGTGGGAAGGGTTGCTGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTT                                                                                                                     <  SRR19973067.3064459/150‑1 (MQ=60)
                                  AGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTT                                                                                                                     <  SRR19973067.1718967/150‑1 (MQ=60)
                                   GGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtt                                                                                                                    >  SRR19973067.3051362/1‑142 (MQ=60)
                                   GGAGTGGGAAGGGTTGCAGGACACCGTCTTGGCCTCGCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTT                                                                                                                    <  SRR19973067.451634/150‑1 (MQ=60)
                                    GTGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                   <  SRR19973067.159649/150‑1 (MQ=60)
                                    GTGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                   <  SRR19973067.3332622/150‑1 (MQ=60)
                                     AGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTG                                                                                                                  <  SRR19973067.199744/150‑1 (MQ=60)
                                        GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgt                                                                                                               >  SRR19973067.2797718/1‑146 (MQ=60)
                                             GGGTTGCTGGACACCGTCTTTGCCTCTCCTCCCTGCCGTGGAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATT                                                                                                          <  SRR19973067.1728685/150‑1 (MQ=60)
                                             GGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATT                                                                                                          <  SRR19973067.3252720/150‑1 (MQ=60)
                                              GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTT                                                                                                         <  SRR19973067.213802/150‑1 (MQ=60)
                                                 TGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGC                                                                                                      <  SRR19973067.3499909/150‑1 (MQ=60)
                                                 TGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGC                                                                                                      <  SRR19973067.1729508/150‑1 (MQ=60)
                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttgtgtgggtttttgggt                                                                                                   >  SRR19973067.982508/1‑125 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgttgttttttggctt                                                                                                  >  SRR19973067.2920403/1‑133 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttggtgttttttgggtt                                                                                                  >  SRR19973067.2919871/1‑133 (MQ=60)
                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTT                                                                                                <  SRR19973067.1101293/150‑1 (MQ=60)
                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTT                                                                                                <  SRR19973067.1101337/150‑1 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                               <  SRR19973067.1284757/150‑1 (MQ=60)
                                                        CACCGACTTTGCCCCTCCTCCCTGTCGTGGAGCTGGACGCAGCGCGTAAAAACGCATTGGCAAGTGTCGGCGCTTTTCCTGTCTGTTGCTAAATATTTTAGGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                               <  SRR19973067.1284907/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                              <  SRR19973067.543518/150‑1 (MQ=60)
                                                           CGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCC                                                                                            <  SRR19973067.2213688/150‑1 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttttgttggtgttggggttttttggc                                                                                           >  SRR19973067.708548/1‑117 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttggtttttgtggttttttggcttttttgcc                                                                                           >  SRR19973067.2571845/1‑118 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtgggtttttgggttttttggc                                                                                           >  SRR19973067.708628/1‑128 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACCGTATGTTGCTAAAAATTTTATGAAAAAAGGCCTGCGGGgcggggggttttttgtttgtttttttggtttttgga                                                                                           >  SRR19973067.919069/1‑114 (MQ=60)
                                                             TCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                          <  SRR19973067.260645/150‑1 (MQ=60)
                                                             TCTTGGCCTCACCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                          <  SRR19973067.2117381/150‑1 (MQ=60)
                                                              CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTGTgtgggttttggggttttttggccg                                                                                         >  SRR19973067.2154988/1‑126 (MQ=60)
                                                                 TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                      <  SRR19973067.3261821/150‑1 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttggttgggtttttgggttttttggcccccgta                                                                                    >  SRR19973067.1664389/1‑110 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTGGTGGATTTTTGGCTTTTTTGCCAGCATTC                                                                                    >  SRR19973067.2388606/1‑150 (MQ=60)
                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA                                                                                   <  SRR19973067.1393636/150‑1 (MQ=60)
                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA                                                                                   <  SRR19973067.1393657/150‑1 (MQ=60)
                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAC                                                                                <  SRR19973067.1192550/150‑1 (MQ=60)
                                                                          TCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttttgggtttttgggttttttggcagccatcagacgaa                                                                             >  SRR19973067.3078486/1‑103 (MQ=60)
                                                                          TCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttgtttgttttttgggttttggccagcaatcagtcgac                                                                             >  SRR19973067.3388522/1‑104 (MQ=60)
                                                                          TCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtggttttttgggttttttgcccccaataagaagaa                                                                             >  SRR19973067.247376/1‑114 (MQ=60)
                                                                           CCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttttgggtttttgggtttttttgcaacaaaataaagaaa                                                                            >  SRR19973067.3367420/1‑103 (MQ=60)
                                                                               GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgttggtttttggggttttttgccgcagtaagacgaaaattt                                                                        >  SRR19973067.2306333/1‑107 (MQ=60)
                                                                                TCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC                                                                      <  SRR19973067.3248057/150‑1 (MQ=60)
                                                                                 CGTGGAGCAGTAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCC                                                                     <  SRR19973067.1598994/150‑1 (MQ=60)
                                                                                 CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCC                                                                     <  SRR19973067.422250/150‑1 (MQ=60)
                                                                                 CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCC                                                                     <  SRR19973067.3368929/150‑1 (MQ=60)
                                                                                 CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCC                                                                     <  SRR19973067.2008040/150‑1 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                    <  SRR19973067.1078212/150‑1 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                    <  SRR19973067.1078257/150‑1 (MQ=60)
                                                                                  GTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTGTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                    <  SRR19973067.2442776/150‑1 (MQ=60)
                                                                                  GTGGAGCAGCAAGCATCGCGTAAAAACCCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCAAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                    <  SRR19973067.1232162/150‑1 (MQ=60)
                                                                                   TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGGGGATTTTTCGCTTTTTGGCCAGCATTCAGACG‑AAAATTGGCCG                                                                   >  SRR19973067.1798709/1‑150 (MQ=60)
                                                                                   TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGTGTTTTGGCGTTTTGCCCAGCAATCAGACG‑AAAAATGGCCG                                                                   >  SRR19973067.1798720/1‑150 (MQ=60)
                                                                                      AGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttgggttttttggccgccttgagaaggaaaattggcgggg                                                                 >  SRR19973067.2864634/1‑100 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACACATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtggtttgtgttggttttggggtttttggccagcaatcagacgaaaaattgcccggggtt                                                              >  SRR19973067.1318397/1‑89 (MQ=60)
                                                                                          GGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTG                                                            <  SRR19973067.1038523/150‑1 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTtttttttgtggttttttgggttttttcccagcattaagaagaaaatttgccggggattggg                                                           >  SRR19973067.574770/1‑89 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGATTTTGCGCGTTTTGCCCCGCATTCAGACG‑AAAAATTCCCGGGACTTGTG                                                          >  SRR19973067.573980/1‑150 (MQ=60)
                                                                                             AGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGA                                                         <  SRR19973067.2518494/150‑1 (MQ=60)
                                                                                                CTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAA                                                      <  SRR19973067.1931293/150‑1 (MQ=60)
                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAA                                                      <  SRR19973067.1931010/150‑1 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtggttttttgggttttttggccgccatgggacgcacaattgccggggcttgtggaaaaatac                                                  >  SRR19973067.1981709/1‑87 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                                 <  SRR19973067.186617/150‑1 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                                 <  SRR19973067.1569463/150‑1 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgggttgtgtgggtttttgggtttttggccacgctacaggagcaaaatgccccgggaatgtgtaaaaatac                                                  >  SRR19973067.1981764/1‑76 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgtgtggtttgtggtggtttttggcttttttgcccccaataataagaaaaattaccggggcatgttgaaaaaaaaa                                                 >  SRR19973067.406703/1‑75 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGTTTTTTGGCTTTTTGGCCAGCAATCAGAAG‑AAAAATGCCCGGGGATTGTGTAAAAATAAG                                                >  SRR19973067.1197576/1‑150 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGGTTTTTGGCTTTTTTCCCAGCATTAAGAAG‑AAAAATTGCCGGGTATTGTGTAAAAATAAG                                                >  SRR19973067.1197706/1‑150 (MQ=60)
                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggggggttttgtgggtttttggggtttttgcccgccatcagtcgcaaaatggccgggtattgtttaaaaataatc                                                >  SRR19973067.1666248/1‑74 (MQ=60)
                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttggggtttttggcagccatcacaccccaaattacccgggcatgtgtaaaaaaaata                                                >  SRR19973067.1666069/1‑83 (MQ=60)
                                                                                                               ATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgtgggttttttggtttttttccacccattatacggaaaattgcccgggagtgtgtaaaaaaaaaagacaaccc                                        >  SRR19973067.722529/1‑66 (MQ=60)
                                                                                                                TTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGTGTTGTTTTGTgtgggttttggggtttttttcccgcactcagacgcaaaaatacccgggagtgtgttaaaaaaaaagcaaccgcc                                       >  SRR19973067.3360794/1‑76 (MQ=60)
                                                                                                                      ACTGTCGGCGCTTTTCCATTATGTTGCAAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAA                                <  SRR19973067.2549182/150‑1 (MQ=60)
                                                                                                                       CTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAC                               <  SRR19973067.2636487/150‑1 (MQ=60)
                                                                                                                        TGTCGGCGATTTTCCATTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACT‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACC                              <  SRR19973067.2557205/150‑1 (MQ=60)
                                                                                                                          tcggctgttttcatgtatttttCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGAATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGT                            <  SRR19973067.373750/128‑1 (MQ=60)
                                                                                                                          TCGGCGATTTTCCTGTTTGTTGATAAAATTTTTATAAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACT‑AAAATGGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGT                            <  SRR19973067.373742/150‑1 (MQ=60)
                                                                                                                             GCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                         <  SRR19973067.1695637/150‑1 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtttttttttttggtttttgggttttttgacaaaattaaaaacaaaaattacccgggcaagtgtaaaaaaaaacaaaagcaccccaaaaaccttaaacacac                    >  SRR19973067.421774/1‑47 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttgtgtgggtttttggctttttttgcaccaatcagaacaaaaattacagggcaaattttaaaaaaaaacaaaacccccccataaacaataaagaaaa                    >  SRR19973067.723786/1‑47 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTGGTTTTttttgggtttttgggttttttgcccccaaataaaacaaaaaatccccggccatttgaaaaaaaaaacgaaaccccgcaaaaaaccataagcacac                    >  SRR19973067.411928/1‑55 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTGGGCTTTTTtgccacccatcagaccaaaaaataccgggaattgtgaaaaaataaccgacaccgcgccaaaaaccatctcgactcaaaa                >  SRR19973067.2299295/1‑71 (MQ=60)
                                                                                                                                         TATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAA             <  SRR19973067.828519/150‑1 (MQ=60)
                                                                                                                                          ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTTGTTTTttgttggtttttggggtttttgcccgccaaaaatacaaaaatggcacggcagtgtttaaaaaaaaaaaaccccccccaaaaaaacctctacacgccaaaaaa             >  SRR19973067.924878/1‑48 (MQ=60)
                                                                                                                                           TGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGT           <  SRR19973067.1785722/150‑1 (MQ=60)
                                                                                                                                           TGTTGATAAAATTTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCTGCATTCAGACG‑AAATTTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGT           <  SRR19973067.2262854/150‑1 (MQ=60)
                                                                                                                                            GTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGATTTTtgggttttttgccaccaatcagacgaaaaaatcccggggaatgtgaaaaaaaaatcgaccgcccgcaaaaaaacatctagaatcaaaaaaatg           >  SRR19973067.2336320/1‑57 (MQ=60)
                                                                                                                                             TTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCTTTTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGA         <  SRR19973067.3374172/150‑1 (MQ=60)
                                                                                                                                                   AAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGTTTTTTGGCTTTTTTCCCAGCATTAAGACGAAAAATTGCCCGGGAA‑TGTGTAAAAATAAACGACAGCGCGCAAaaaaccgtctcgacgcataaaagttattaccc   >  SRR19973067.3540129/1‑118 (MQ=36)
                                                                                                                                                    AAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCT  <  SRR19973067.1983084/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCT  >  CP009273/450141‑450438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑