Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	450,290	G→A	100%	intergenic (+45/‑299)	bolA → / → tig	stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	450,290	0	G	A	94.7%	792.4 / 24.0	229	intergenic (+45/‑299)	bolA/tig	stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand): ref base G (7/5); new base A (95/121); total (102/127)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 230 total aligned reads displayed.

TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450142‑450435

ttCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGa                                                                                                                                                     >  2:806393/1‑150 (MQ=255)

ttCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGa                                                                                                                                                     >  2:806251/1‑150 (MQ=255)

  cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   >  1:1490876/1‑150 (MQ=255)

  cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   <  1:2726231/150‑1 (MQ=255)

    tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                 >  2:2340957/1‑150 (MQ=255)

        cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             <  1:3220592/150‑1 (MQ=255)

        cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             >  1:2997756/1‑150 (MQ=255)

           gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                          >  2:3859667/1‑150 (MQ=255)

            ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         <  2:3799955/150‑1 (MQ=255)

            ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         >  2:1720949/1‑150 (MQ=255)

             tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                        <  1:1152220/150‑1 (MQ=255)

              ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                       <  1:2952820/150‑1 (MQ=255)

                 cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    >  2:908548/1‑150 (MQ=255)

                    aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGc                                                                                                                                 >  1:1798374/1‑150 (MQ=255)

                    aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                                 >  1:3167472/1‑150 (MQ=255)

                    aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                                 >  1:4109637/1‑150 (MQ=255)

                      ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAAGGCCTGCgg                                                                                                                               >  1:880283/1‑150 (MQ=255)

                      ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCgg                                                                                                                               <  2:1084655/150‑1 (MQ=255)

                        acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                             >  2:3641853/1‑150 (MQ=255)

                         cacTATTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                            <  1:1284279/150‑1 (MQ=255)

                          acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCg                                                                                                                           >  2:3849816/1‑150 (MQ=255)

                          acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCg                                                                                                                           <  2:619921/150‑1 (MQ=255)

                              ttAACGTGTGGGCAGGGTTGCAGGACACCGACTTTGCCACTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTACCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtttt                                                                                                                       <  1:3267898/150‑1 (MQ=255)

                                aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttgt                                                                                                                     >  2:1311132/1‑150 (MQ=255)

                                aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttgg                                                                                                                     >  1:1023215/1‑149 (MQ=255)

                                  ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGGCgtgttgttt                                                                                                                   >  1:3337638/1‑150 (MQ=255)

                                           aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGG‑At                                                                                                         >  1:3978768/1‑150 (MQ=255)

                                           aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑At                                                                                                         <  2:2697745/150‑1 (MQ=255)

                                           aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑At                                                                                                         >  2:1033140/1‑150 (MQ=255)

                                            gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Att                                                                                                        <  2:3037583/150‑1 (MQ=255)

                                            gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Att                                                                                                        <  2:3234742/150‑1 (MQ=255)

                                            gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Att                                                                                                        <  1:2188410/150‑1 (MQ=255)

                                             ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Attt                                                                                                       <  1:485342/150‑1 (MQ=255)

                                             ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTTG‑Attt                                                                                                       >  2:2843414/1‑150 (MQ=255)

                                              gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑Atttt                                                                                                      <  2:3556325/150‑1 (MQ=255)

                                                       taaggTCTTTGCCTCTCCTCCCTGTCTTGGAGCATGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCttttt                                                                                             <  2:1378495/146‑1 (MQ=255)

                                                       taaggTCTTTGCCTCTCCTCCCGGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCttttt                                                                                             <  2:3325169/146‑1 (MQ=255)

                                                       cacCGTCTTTGCCACTCCTCCCTGTCGTGGAGCAGTAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCttttt                                                                                             <  1:1916792/150‑1 (MQ=255)

                                                        acCGTCTGTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTg                                                                                            <  1:2977773/150‑1 (MQ=255)

                                                          cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGcc                                                                                          <  1:1077378/150‑1 (MQ=255)

                                                           gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTTGGGGTTTTTGcc                                                                                          >  1:874171/1‑150 (MQ=255)

                                                               ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGG‑GTTTTTGGCTTTTTTGCCAGC‑a                                                                                    >  1:2050345/1‑150 (MQ=255)

                                                                tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑At                                                                                   >  2:1671113/1‑150 (MQ=255)

                                                                tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGG‑ATTTTGGGCTTTTTTCCCaccca                                                                                    >  1:987707/1‑146 (MQ=255)

                                                                  ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGGTTTTTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTc                                                                                 >  2:1932235/1‑150 (MQ=255)

                                                                   ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGG‑ATTTTGCGCGTTTTGCCCAGC‑ATTCa                                                                                >  2:3393069/1‑150 (MQ=255)

                                                                    tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAg                                                                               <  2:1828596/150‑1 (MQ=255)

                                                                       cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGG‑GTTTTTGGGTTTTTGACCCGC‑AATCAGACg                                                                            >  1:1680031/1‑150 (MQ=255)

                                                                        ctccCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGa                                                                           >  2:2505267/1‑150 (MQ=255)

                                                                        ctccCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGa                                                                           >  2:330789/1‑150 (MQ=255)

                                                                           ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGaaaa                                                                        <  1:2537843/150‑1 (MQ=255)

                                                                             tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAt‑t                                                                     <  1:2305069/150‑1 (MQ=255)

                                                                             tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAt‑t                                                                     <  1:3633304/150‑1 (MQ=255)

                                                                              gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑Tg                                                                    <  1:970510/150‑1 (MQ=255)

                                                                              gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCCGC‑AATCAGACGAAAAT‑Tg                                                                    >  2:301382/1‑150 (MQ=255)

                                                                              gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑Tg                                                                    <  1:3753774/150‑1 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGG‑ATTTTTCGCTTTTTGGCCAGC‑ATTCAGACGCAAAT‑TGcc                                                                  >  2:3380976/1‑150 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑CTTCAGACGAAAAG‑TGcc                                                                  >  2:3243937/1‑150 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGcc                                                                  <  1:1639000/150‑1 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGcc                                                                  <  1:792650/150‑1 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGG‑TTTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGcc                                                                  >  2:3605868/1‑150 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTTG‑GTTTTTGGCTTTTTTACCACC‑ATTGAGACGaaaaaagcc                                                                   >  2:1811960/1‑145 (MQ=255)

                                                                                cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑CTTCAGACGCAAAA‑TGcc                                                                  >  2:2294636/1‑150 (MQ=255)

                                                                                 gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGccc                                                                 <  1:2168751/150‑1 (MQ=255)

                                                                                  tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCg                                                                <  1:3047883/150‑1 (MQ=255)

                                                                                  tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTTGGGTTTTTTGCCC‑GC‑ATTAAGGCGGAAAT‑Tggcgg                                                                >  1:3899098/1‑146 (MQ=255)

                                                                                   ggAGCAGGAAGCATCGCGTAAAAACGCATGTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCgg                                                               <  1:2581949/150‑1 (MQ=255)

                                                                                    gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGG‑ATTTTGGGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCggg                                                              >  2:214311/1‑150 (MQ=255)

                                                                                     aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGa                                                             <  1:1798840/150‑1 (MQ=255)

                                                                                        aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAAtt                                                          <  2:1757292/150‑1 (MQ=255)

                                                                                         ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGG‑ATTTTGCGCTTTTTTCCCAGC‑ATTCAGTCGAAAAT‑TGCCCGGGAATtg                                                         >  2:1743444/1‑150 (MQ=255)

                                                                                            aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGa                                                      <  1:1012570/150‑1 (MQ=255)

                                                                                             gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCGGTTTTTTTTTGTGGGG‑TTTTTGGGGGTTTTTGCCACC‑AATCAGGCGATAAT‑TGGCCGGGTATagttta                                                     >  1:583392/1‑147 (MQ=255)

                                                                                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAAt                                                <  1:3129329/150‑1 (MQ=255)

                                                                                                  gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAAt                                                <  1:3201745/150‑1 (MQ=255)

                                                                                                   cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTTCGCTTTTTTCCCAGC‑ATTAAGACGAAAAT‑TTGCCGGGAATTGTGAAAAAATa                                               >  2:4086974/1‑150 (MQ=255)

                                                                                                   cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATa                                               <  1:4321408/150‑1 (MQ=255)

                                                                                                    gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATAc                                              <  2:638574/150‑1 (MQ=255)

                                                                                                         caCGCATGTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGAc                                         <  2:2205867/149‑1 (MQ=255)

                                                                                                         aaCGCATTTGCAACTGTCGGCGCTTTTCCATTATTTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGAc                                         <  2:2206292/150‑1 (MQ=255)

                                                                                                            gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTTGTGG‑GTTTTTGGGTTTTTTGGCACC‑CAACAGACGAAAATATGCCCGGG‑ATTGTGTAAAAAAAAGCGCCacc                                      >  1:1201925/1‑148 (MQ=255)

                                                                                                              aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTTCCCAGC‑AATAAGACCAAAAA‑TGCCCCGGGATTGTGAAAAAAAACGCCACAccgc                                    >  2:2622929/1‑150 (MQ=255)

                                                                                                                    aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                              <  2:1233497/150‑1 (MQ=255)

                                                                                                                      cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                            <  2:1658398/150‑1 (MQ=255)

                                                                                                                           ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTATGG‑ATTTTTGGCTTTTTGCCCACC‑ATTCAGACCAAAAA‑TGACCGGGAATTTTGTAAAAATAAGCGACAGCGCGCAAAAACCCTtc                       >  2:1267688/1‑150 (MQ=255)

                                                                                                                           ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                       <  2:2794246/150‑1 (MQ=255)

                                                                                                                            gcgcTTTGACAGTATGTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                      <  1:4246642/150‑1 (MQ=255)

                                                                                                                              gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTTG‑ATTTTTCGCTTTTTGCCCACC‑AATCAGAACAAAAT‑TTCCCGGGAATTGTGAAAAAAAAAGCGACAGCGCGCAATAAcccatctag                    >  2:2258779/1‑143 (MQ=255)

                                                                                                                               cTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                   <  2:1503294/150‑1 (MQ=255)

                                                                                                                                ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTTCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGAc                  >  2:3939938/1‑150 (MQ=255)

                                                                                                                                ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGTGG‑ATTTTTCGCTTTTTTACCAGCAATACGCACAAAAAT‑TCCCCGGGAA‑TGTTAAAAAAAAAGCGAACCCGCCCAAAAACCGATCTCGAc                  >  2:1116796/1‑150 (MQ=255)

                                                                                                                                 tttCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt                 <  1:106665/150‑1 (MQ=255)

                                                                                                                                    ccAGTATGTTGCTAAAAATGTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTAGGG‑ATTTTGCGCTTTTTTCCCAGC‑ATTCAGACGAAAAG‑TGCCCGGGAATTTTGTAAAAATACGCGGAAGCGCGCACAAACCGGTATCGACTCAt              >  2:3531495/1‑150 (MQ=255)

                                                                                                                                     ctgtttGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  1:3784832/145‑1 (MQ=255)

                                                                                                                                     cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  2:1198702/150‑1 (MQ=255)

                                                                                                                                     cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  2:207284/150‑1 (MQ=255)

                                                                                                                                       gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa           <  2:2627069/150‑1 (MQ=255)

                                                                                                                                           gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg       <  2:2606231/150‑1 (MQ=255)

                                                                                                                                            tggCTAAAATTTTTATTAAAAACGGCCGGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAATT‑TGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa      <  1:3288990/148‑1 (MQ=255)

                                                                                                                                               cTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTTTCTGG‑ATTTTTCGCGTTTTTCCCAGC‑AATAAGACGAAAAT‑TGGCCGGGCATTGTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACTCAAAAAAGTGATGc   >  2:3700635/1‑150 (MQ=255)

TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG‑ATTTTGCGCTTTTTGCCCAGC‑ATTCAGACGAAAAT‑TGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450142‑450435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG  >  CP009273/450142‑450433

TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                >  SRR19973079.2287244/1‑150 (MQ=60)

 TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGAT                                                                                                                                               <  SRR19973079.2270083/150‑1 (MQ=60)

 TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAT                                                                                                                                               <  SRR19973079.1982628/150‑1 (MQ=60)

  CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                              <  SRR19973079.2740325/150‑1 (MQ=60)

  CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                              >  SRR19973079.1499081/1‑150 (MQ=60)

  CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                              >  SRR19973079.1499074/1‑150 (MQ=60)

      CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                          >  SRR19973079.3211202/1‑150 (MQ=60)

        CTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGA                                                                                                                                        <  SRR19973079.3236966/150‑1 (MQ=60)

        CTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGA                                                                                                                                        >  SRR19973079.3013230/1‑150 (MQ=60)

             TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                   <  SRR19973079.1158307/150‑1 (MQ=60)

             TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                   <  SRR19973079.3641706/150‑1 (MQ=60)

             TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                   <  SRR19973079.1158836/150‑1 (MQ=60)

              CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                  <  SRR19973079.2967973/150‑1 (MQ=60)

               TGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGG                                                                                                                                 <  SRR19973079.1803554/150‑1 (MQ=60)

               TGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGG                                                                                                                                 <  SRR19973079.1803068/150‑1 (MQ=60)

                 CATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCC                                                                                                                               >  SRR19973079.3987775/1‑150 (MQ=60)

                    ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGC                                                                                                                            >  SRR19973079.1807741/1‑150 (MQ=60)

                    ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                            >  SRR19973079.3183699/1‑150 (MQ=60)

                    ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                            >  SRR19973079.4130479/1‑150 (MQ=60)

                      TTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAAAGGCCTGCGG                                                                                                                          >  SRR19973079.885378/1‑150 (MQ=60)

                         CACTATTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCC                                                                                                                       <  SRR19973079.1291593/150‑1 (MQ=60)

                            TATTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCCCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                    <  SRR19973079.3138440/150‑1 (MQ=60)

                              TTAAGGAGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTT                                                                                                                  <  SRR19973079.948827/150‑1 (MQ=60)

                              TTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTT                                                                                                                  <  SRR19973079.3284250/150‑1 (MQ=60)

                              TTAACGTGTGGGCAGGGTTGCAGGACACCGACTTTGCCACTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTACCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTT                                                                                                                  <  SRR19973079.3284383/150‑1 (MQ=60)

                                AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttgg                                                                                                                >  SRR19973079.1028928/1‑145 (MQ=60)

                                AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGT                                                                                                                >  SRR19973079.1028739/1‑150 (MQ=60)

                                  GGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTT                                                                                                              <  SRR19973079.4219853/150‑1 (MQ=60)

                                  GGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGGCGTGTTGTTT                                                                                                              >  SRR19973079.3354750/1‑150 (MQ=60)

                                  GGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTT                                                                                                              >  SRR19973079.1147772/1‑150 (MQ=60)

                                  GGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCACCTCCCTGTCGTGGAGCAGGAAGAATCGCTTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTT                                                                                                              <  SRR19973079.413551/150‑1 (MQ=60)

                                           AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGAT                                                                                                     >  SRR19973079.3998767/1‑150 (MQ=60)

                                            GGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATT                                                                                                    <  SRR19973079.2199646/150‑1 (MQ=60)

                                            GGGTGGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAACCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATT                                                                                                    <  SRR19973079.2198323/150‑1 (MQ=60)

                                             GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTT                                                                                                   <  SRR19973079.488292/150‑1 (MQ=60)

                                             GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTT                                                                                                   <  SRR19973079.488433/150‑1 (MQ=60)

                                               TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttgtgtttt                                                                                                 >  SRR19973079.870852/1‑130 (MQ=60)

                                               TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTgttgtgttttgtgcttttt                                                                                                 >  SRR19973079.870523/1‑131 (MQ=60)

                                               TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATGAAAAAAGGCCTGCGGGCCGgggttgttttttttgtttttt                                                                                                 >  SRR19973079.870813/1‑129 (MQ=60)

                                                TGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAACGGCCGGCGGGCCGTGTTGTTTTttgttgttttttt                                                                                                >  SRR19973079.4140347/1‑137 (MQ=60)

                                                TGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTtttttgttttttg                                                                                                >  SRR19973079.2628774/1‑137 (MQ=60)

                                                   AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtggttgttggtttttttggt                                                                                             >  SRR19973079.2640639/1‑126 (MQ=60)

                                                   AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtgggtttttgggg                                                                                             >  SRR19973079.3082059/1‑126 (MQ=60)

                                                      ACACCGTCTTTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTT                                                                                          <  SRR19973079.2823805/150‑1 (MQ=60)

                                                       CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                         <  SRR19973079.4377826/150‑1 (MQ=60)

                                                       CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                         <  SRR19973079.2541566/150‑1 (MQ=60)

                                                       CACCGTCTTTGCCACTCCTCCCTGTCGTGGAGCAGTAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                         <  SRR19973079.1927027/150‑1 (MQ=60)

                                                        ACCGTCTGTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                        <  SRR19973079.2993094/150‑1 (MQ=60)

                                                        ACCGTATTTGCCTCTCCTCCCTGTCGTGTAGCAGTAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGATTTTCCTTTTTTTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                        <  SRR19973079.387274/150‑1 (MQ=60)

                                                          CGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCC                                                                                      <  SRR19973079.1083285/150‑1 (MQ=60)

                                                          CGTCTTTGCCACTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTACCATTTTTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCC                                                                                      <  SRR19973079.1083248/150‑1 (MQ=60)

                                                           GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTtttgtgggttttggggtttttgcc                                                                                     >  SRR19973079.2562987/1‑126 (MQ=60)

                                                           GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtggttttttggggtttttgcc                                                                                     >  SRR19973079.879237/1‑128 (MQ=60)

                                                           GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgtggggtgttgtctttttttgc                                                                                     >  SRR19973079.376353/1‑117 (MQ=60)

                                                            TCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggggtttttttttgtgttttggcgtttttggca                                                                                    >  SRR19973079.3369402/1‑117 (MQ=60)

                                                               TTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttggcttttttgccagca                                                                                 >  SRR19973079.2061101/1‑122 (MQ=60)

                                                               TTGCCTCTCCTCCCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCA                                                                                 <  SRR19973079.2098771/150‑1 (MQ=60)

                                                                TGCCTCTCCTCCCTGTCGTGGAGCTGGAAGAATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                <  SRR19973079.3322218/150‑1 (MQ=60)

                                                                TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                <  SRR19973079.3784150/150‑1 (MQ=60)

                                                                TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                <  SRR19973079.2685290/150‑1 (MQ=60)

                                                                TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCGGGATTTTGGGCTTTTTtcccaccca                                                                                >  SRR19973079.993297/1‑141 (MQ=60)

                                                                     CTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA                                                                           <  SRR19973079.2844199/150‑1 (MQ=60)

                                                                       CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAATCGGCCTGCGGGCCGTGTTGTTTTttgttgtttttttgggtttttgaccgccataagaaa                                                                         >  SRR19973079.428420/1‑114 (MQ=60)

                                                                       CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgttgatttttcgcttttttaccagccataagacg                                                                         >  SRR19973079.1689072/1‑114 (MQ=60)

                                                                       CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtgggtttttgggtttttgacccgcaatcagacg                                                                         >  SRR19973079.1689084/1‑116 (MQ=60)

                                                                        CTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGgggtgtttttttttggttttgtgcgttttttccaccaaaaatacca                                                                        >  SRR19973079.124174/1‑104 (MQ=60)

                                                                          CCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAA                                                                      <  SRR19973079.3012703/150‑1 (MQ=60)

                                                                           CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAA                                                                     <  SRR19973079.2550636/150‑1 (MQ=60)

                                                                             TGTCGTGGAGCAGTAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATT                                                                   <  SRR19973079.3651682/150‑1 (MQ=60)

                                                                             TGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATT                                                                   <  SRR19973079.2317033/150‑1 (MQ=60)

                                                                              GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTtttttttatggttttttggcttttttcccagcattaataagaaaaatg                                                                  >  SRR19973079.1046576/1‑102 (MQ=60)

                                                                              GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtggttttttgggttttttgcccaccataaaaagaaaaatt                                                                  >  SRR19973079.4209680/1‑107 (MQ=60)

                                                                              GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                  <  SRR19973079.976065/150‑1 (MQ=60)

                                                                              GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                  <  SRR19973079.976033/150‑1 (MQ=60)

                                                                              GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                  <  SRR19973079.3773007/150‑1 (MQ=60)

                                                                                CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCC                                                                <  SRR19973079.797149/150‑1 (MQ=60)

                                                                                CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCC                                                                <  SRR19973079.1647942/150‑1 (MQ=60)

                                                                                 GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCC                                                               <  SRR19973079.2179829/150‑1 (MQ=60)

                                                                                  TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCG                                                              <  SRR19973079.3063664/150‑1 (MQ=60)

                                                                                  TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtggttttttgggttttttgcccgcattaaggcggaaattggcgg                                                              >  SRR19973079.3918878/1‑105 (MQ=60)

                                                                                   GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggggtttttttttgtttttgtgggtttttgacagcagtaagacggaaaaatgacgg                                                             >  SRR19973079.1173184/1‑94 (MQ=60)

                                                                                   GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGG                                                             <  SRR19973079.3227866/150‑1 (MQ=60)

                                                                                   GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttggcgttttggccagcaatcagacgcaaaatgaccgg                                                             >  SRR19973079.1259891/1‑102 (MQ=60)

                                                                                   GGAGCAGGAAGCATCGCGTAAAAACGCATGTGCAACTGGCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGG                                                             <  SRR19973079.2595163/150‑1 (MQ=60)

                                                                                     AGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGA                                                           <  SRR19973079.1808208/150‑1 (MQ=60)

                                                                                     AGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGA                                                           <  SRR19973079.1808204/150‑1 (MQ=60)

                                                                                      GCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAA                                                          <  SRR19973079.1381422/150‑1 (MQ=60)

                                                                                        AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                        <  SRR19973079.82711/150‑1 (MQ=60)

                                                                                        AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgtttttttttggtttttggggttttttgccaccaaaaaactcaaaatttaccgggatt                                                        >  SRR19973079.1571655/1‑88 (MQ=60)

                                                                                          GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttttgttgttttttggggttttgtggcgccgtgttgagaaaattgggcggggcttgg                                                      >  SRR19973079.2563016/1‑86 (MQ=60)

                                                                                          GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGGTGCGAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggggggttgtgtggggttggggggggttgtcccgccggccgccggcaaatgcgcgcggcgggg                                                      >  SRR19973079.2563021/1‑86 (MQ=60)

                                                                                            AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGA                                                    <  SRR19973079.2984265/150‑1 (MQ=60)

                                                                                            AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGA                                                    <  SRR19973079.1018258/150‑1 (MQ=60)

                                                                                            AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGA                                                    <  SRR19973079.879559/150‑1 (MQ=60)

                                                                                             GCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCGGTTTTTTTTTGTggggtttttgggggtttttgccaccaatcaggcgataattggccgggtatagttta                                                   >  SRR19973079.586821/1‑94 (MQ=60)

                                                                                               ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTgtttttgttttttgggttttggggtttttggcccccaaaagaccaacaagtgaccgggcgttggtaaa                                                 >  SRR19973079.1455870/1‑82 (MQ=60)

                                                                                                  GCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAT                                              <  SRR19973079.3218080/150‑1 (MQ=60)

                                                                                                  GCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAT                                              <  SRR19973079.3224442/150‑1 (MQ=60)

                                                                                                  GCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAT                                              <  SRR19973079.3145437/150‑1 (MQ=60)

                                                                                                   CTTAAAAACGCATTTGCACCGGTCGGCGCTTTTCCAGTATTTTTATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATA                                             <  SRR19973079.2370604/150‑1 (MQ=60)

                                                                                                   CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttttgggttttttgggttttttccccccatgagcccaacaattacagggcaatgtgtaaaaaaa                                             >  SRR19973079.1492997/1‑78 (MQ=60)

                                                                                                   CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATA                                             <  SRR19973079.4343145/150‑1 (MQ=60)

                                                                                                   CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATA                                             <  SRR19973079.4343137/150‑1 (MQ=60)

                                                                                                       AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgtttttggtggggttttgggttttttgccaccaaaaaaacgcaaatttcccgggcatttttgaaaaaaaaccg                                         >  SRR19973079.3830169/1‑73 (MQ=60)

                                                                                                        AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttgttttttttggtttttggggttttttgcggcaaaggaaacggaaatttgcggggagttttttaaaaaaagaga                                        >  SRR19973079.4034072/1‑73 (MQ=60)

                                                                                                        AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttggggtttttgcccgccttaaaacgaaaaaatgccgggcagtttgtaaaaaaaagcga                                        >  SRR19973079.1197320/1‑81 (MQ=60)

                                                                                                        AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTtttggttttggggttttttgcccgccttaaaaagaaaaattgccgggcaatttgtaaaaaaaaaaga                                        >  SRR19973079.553496/1‑83 (MQ=60)

                                                                                                            GCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttgtgggtttttgggttttttggcacccaacagacgaaaatatgcccgggattgtgtaaaaaaaagcgccacc                                    >  SRR19973079.1208841/1‑69 (MQ=60)

                                                                                                              ATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggggtgttttgggggtttttggggtttttgacacccatgagaaggacaatggcccggaaatttgtaaaaaaaaacgcccaagc                                  >  SRR19973079.820062/1‑66 (MQ=60)

                                                                                                              ATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTggggttttttggcttttttcccaccaatcaatcgaaacattgccgggcaatgtgtaaaaataacacgcaccgc                                  >  SRR19973079.4225010/1‑77 (MQ=60)

                                                                                                              ATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggtggttttttgtggtttttttggggtttttaccgcaataagaaccaaaattaccggggcttttttgaaaaaaatagacacacc                                  >  SRR19973079.3008211/1‑66 (MQ=60)

                                                                                                              ATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtttttttttttgtttttttgggttttttacacccataagtagaaaaattggcggggcatgtttataaaaaaaagaccgggc                                  >  SRR19973079.1872384/1‑67 (MQ=60)

                                                                                                                       TGTCGGCGCTTTCCCTGTATGTTTATAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACC                         <  SRR19973079.3717273/150‑1 (MQ=60)

                                                                                                                        GTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTtttttttgttgttttttggcttttttgccagcaataagtcgaaaaattacccgggaatttgaaaaaaaaaaccaaaacgccccaaaaaac                        >  SRR19973079.697936/1‑60 (MQ=60)

                                                                                                                          CGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttttggtttttttgggttttttacagcaataattagaaaaaatgcccgggcttttgttaaaataaaccacaccgcgcaaaaaacaga                      >  SRR19973079.828591/1‑55 (MQ=60)

                                                                                                                          CGGCGATTTTCCATTTTGTTGATAAATATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTT                      <  SRR19973079.3145558/150‑1 (MQ=60)

                                                                                                                            GCGCTTTGACAGTATGTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                    <  SRR19973079.4268132/150‑1 (MQ=60)

                                                                                                                             CGCTTTTCCAGTTTGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                   <  SRR19973079.1179575/150‑1 (MQ=60)

                                                                                                                               tttttcattttTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGA                 <  SRR19973079.3660343/139‑1 (MQ=60)

                                                                                                                                 TTTCCTTTATGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACT               <  SRR19973079.3033228/150‑1 (MQ=60)

                                                                                                                                 TTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACT               <  SRR19973079.107593/150‑1 (MQ=60)

                                                                                                                                  TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTGGGTTTTGCGCTTTTTtccccgcaataacacgacaaaatcccgggcatttttgaaaaaaaagcgacacagcgccaaaaaccaacgcgacac              >  SRR19973079.1233142/1‑75 (MQ=60)

                                                                                                                                     CAGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATA           <  SRR19973079.1353831/150‑1 (MQ=60)

                                                                                                                                         TTGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAG       <  SRR19973079.411679/150‑1 (MQ=60)

                                                                                                                                         ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGATTTTTCGCTTTTTtccccccattaagacgaaaaattacccgggaatgttaaaaaaaaaacgacagcgcgcaaaaaaccttctcgacaaaaaaaaa       >  SRR19973079.3622998/1‑68 (MQ=60)

                                                                                                                                         ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAG       <  SRR19973079.723931/150‑1 (MQ=60)

                                                                                                                                          TGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttttttttggggatttttggcttttttcccaccaataagacgaaaaatggcccggcaatgtttaaaaaaaagcgacaccgcgcaaaaaaccgtctcgactaataaaagt      >  SRR19973079.2097382/1‑39 (MQ=60)

                                                                                                                                          TGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgtgggtttttcgcttttttgccccccataagacgaaaattgccccgggagtttgaaaaaaaaagcgaccacgcgccaaaaaccatatcgacacaaaaaaag      >  SRR19973079.2097333/1‑47 (MQ=60)

                                                                                                                                            TTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGA    <  SRR19973079.1812033/150‑1 (MQ=60)

                                                                                                                                             TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgttgttttttggcttttttgccagcaatcagaagaaaaattcccgggaattgtgtaaaaataaaccacaacccccaaaaaaccttatagaatcaaaaaaatgat   >  SRR19973079.1579061/1‑44 (MQ=60)

                                                                                                                                             TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTttgtgggttttggggttttttgcccgcattcatacgaaaaattgccggggcttgtttaaaaaaaaaagaaaccgcgccaaaaacggtctcgacagaataaagtgtt   >  SRR19973079.1580531/1‑44 (MQ=60)

                                                                                                                                              GCTAAAAATTTTATGAAAAACGGCCTGCTGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG  <  SRR19973079.911354/150‑1 (MQ=60)

TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG  >  CP009273/450142‑450433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`