Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	450,290	G→A	intergenic (+45/‑299)	bolA → / → tig	stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	450,290	0	G	A	99.5%	728.4 / NA	218	intergenic (+45/‑299)	bolA/tig	stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (115/102); minor base T (0/1); total (115/103)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450144‑450436

cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                  <  2:2369089/150‑1 (MQ=255)

cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                  >  1:846792/1‑150 (MQ=255)

    cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                              <  1:1433818/150‑1 (MQ=255)

       tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑aa                                                                                                                                          >  1:3588461/1‑150 (MQ=255)

        ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑aaa                                                                                                                                         >  1:1110159/1‑150 (MQ=255)

          ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑aaaaa                                                                                                                                       <  2:3675321/150‑1 (MQ=255)

            ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACg                                                                                                                                     <  2:2263720/150‑1 (MQ=255)

            ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACg                                                                                                                                     <  1:305014/150‑1 (MQ=255)

             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTATAAATTTTATG‑AAAAACgg                                                                                                                                    >  2:485824/1‑150 (MQ=255)

             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACgg                                                                                                                                    <  2:134417/150‑1 (MQ=255)

             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACgg                                                                                                                                    >  2:498024/1‑150 (MQ=255)

                aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCt                                                                                                                                 <  1:1268194/150‑1 (MQ=255)

                 tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTg                                                                                                                                >  2:12542/1‑150 (MQ=255)

                 tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTg                                                                                                                                <  2:790839/150‑1 (MQ=255)

                     tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCggg                                                                                                                            <  1:2952319/150‑1 (MQ=255)

                      acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGc                                                                                                                           >  2:1387990/1‑150 (MQ=255)

                      acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGc                                                                                                                           >  2:315595/1‑150 (MQ=255)

                      acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGc                                                                                                                           <  1:149040/150‑1 (MQ=255)

                        acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCg                                                                                                                         >  1:3771214/1‑150 (MQ=255)

                        acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCACGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCg                                                                                                                         >  1:1820224/1‑150 (MQ=255)

                         cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgt                                                                                                                        >  2:3327607/1‑150 (MQ=255)

                         cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTACG‑AAAAACGGCCTGCGGGCCgt                                                                                                                        >  1:2684182/1‑150 (MQ=255)

                          tATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgtt                                                                                                                       >  1:3510689/1‑150 (MQ=255)

                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttt                                                                                                                      >  1:1049229/1‑150 (MQ=255)

                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttt                                                                                                                      <  2:2628671/150‑1 (MQ=255)

                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttt                                                                                                                      >  1:1481457/1‑150 (MQ=255)

                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttt                                                                                                                      >  1:2019032/1‑150 (MQ=255)

                             tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgtgttg                                                                                                                    >  1:3616936/1‑150 (MQ=255)

                             tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCATTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttttg                                                                                                                    <  1:3098899/150‑1 (MQ=255)

                               aGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttttgtt                                                                                                                  <  2:1708226/150‑1 (MQ=255)

                                 taGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttttgtttt                                                                                                                >  1:3659466/2‑150 (MQ=255)

                                 gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCgttttgtttt                                                                                                                <  1:3060460/150‑1 (MQ=255)

                                   gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTtttgttttgt                                                                                                              >  2:1932002/1‑150 (MQ=255)

                                        aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGa                                                                                                         >  2:3416535/1‑150 (MQ=255)

                                         aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAt                                                                                                        >  2:3272271/1‑150 (MQ=255)

                                             ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTg                                                                                                    >  2:1768187/1‑150 (MQ=255)

                                              tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                                                   >  2:3711236/1‑150 (MQ=255)

                                               gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcg                                                                                                  <  1:1550453/150‑1 (MQ=255)

                                                cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTtcgc                                                                                                 >  1:188236/1‑150 (MQ=255)

                                                 aGGACACCGTCTTTGCCTCTCCTTCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCt                                                                                                >  1:2443811/1‑150 (MQ=255)

                                                 aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCt                                                                                                >  1:692360/1‑150 (MQ=255)

                                                  ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                               >  2:2183795/1‑150 (MQ=255)

                                                    acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                                             <  2:2029770/150‑1 (MQ=255)

                                                    acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtttt                                                                                             <  2:1658872/150‑1 (MQ=255)

                                                     cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                            <  2:2230232/150‑1 (MQ=255)

                                                     cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                            <  2:2642063/150‑1 (MQ=255)

                                                     cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                            >  1:120979/1‑150 (MQ=255)

                                                      acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                           <  2:878349/150‑1 (MQ=255)

                                                      acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTg                                                                                           >  1:472502/1‑150 (MQ=255)

                                                           cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                      >  2:2143164/1‑150 (MQ=255)

                                                              tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGACCAGCAt                                                                                   >  1:2009442/1‑150 (MQ=255)

                                                              tGCCTCTCCTCCCTGTCGTGGAGCAGGAAACATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                   <  2:2664666/150‑1 (MQ=255)

                                                                ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                 >  1:636843/1‑150 (MQ=255)

                                                                ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                 <  2:652963/150‑1 (MQ=255)

                                                                ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                 <  1:2390147/150‑1 (MQ=255)

                                                                 ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                <  2:115306/150‑1 (MQ=255)

                                                                 ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                <  1:2541451/150‑1 (MQ=255)

                                                                  tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                               <  1:492188/150‑1 (MQ=255)

                                                                    tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAc                                                                             >  1:613456/1‑150 (MQ=255)

                                                                       tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                                          <  1:2326168/150‑1 (MQ=255)

                                                                        cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                         <  2:2218049/150‑1 (MQ=255)

                                                                          cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAt                                                                       <  2:1836075/150‑1 (MQ=255)

                                                                            gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTGTTGTTTTGTATGGATTTTTCGCTTTTTGCCCAGCAATAAGACGAAAAttt                                                                     >  1:131767/1‑149 (MQ=255)

                                                                               gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAGTGccc                                                                  >  2:965834/1‑150 (MQ=255)

                                                                                tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGAGCTTTTTTCCCAGCATTAAGACGAAAATTGCCCg                                                                 >  1:1792580/1‑150 (MQ=255)

                                                                                       ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGC‑TTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                         >  2:2084878/1‑150 (MQ=255)

                                                                                        gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                         >  2:2751249/1‑150 (MQ=255)

                                                                                            cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                     <  2:304225/150‑1 (MQ=255)

                                                                                             aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                     <  2:3543955/150‑1 (MQ=255)

                                                                                             aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaa                                                    >  2:2997917/1‑150 (MQ=255)

                                                                                                gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                 <  2:846792/150‑1 (MQ=255)

                                                                                                gcgTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                 <  1:3379379/150‑1 (MQ=255)

                                                                                                   tAAAAACGCATTTGCATCTGTTGGCGATTTTCTAGTATGTTGCTAAATATTTTATG‑AAAAATGGTGTGCGGGCATTTTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCATTCAGAAGAAAATTGCCCGGGATTTGTGAAAAAATAcg                                              <  1:3688931/150‑1 (MQ=255)

                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                                             >  2:1027787/1‑150 (MQ=255)

                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                                             >  1:3101704/1‑150 (MQ=255)

                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                                             <  1:2751249/150‑1 (MQ=255)

                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTCAGACAAAAATTGACCGGGAATTGTGAAAAAATAagc                                             >  1:3537594/1‑150 (MQ=255)

                                                                                                     aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                            <  1:543857/150‑1 (MQ=255)

                                                                                                      aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAATGTGAAAAAATACGCGa                                           >  1:3714057/1‑150 (MQ=255)

                                                                                                         cGCATGTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAg                                        <  1:2914104/150‑1 (MQ=255)

                                                                                                            aTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAAATGCCCGGGAATTGTGAAAAAATAAGCGACAacgc                                     >  1:2708104/1‑150 (MQ=255)

                                                                                                               tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                  >  1:1249824/1‑150 (MQ=255)

                                                                                                                gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                 <  2:2968983/150‑1 (MQ=255)

                                                                                                                 cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                                >  2:701249/1‑150 (MQ=255)

                                                                                                                  aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                               <  2:2919755/150‑1 (MQ=255)

                                                                                                                   aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTGCGCTTTTTTACCAGCAATAAAACGAAAAATTCCCGGGAAATGTGAAAAAATAAACGACAACGCGCAaaaa                              >  1:1699318/1‑150 (MQ=255)

                                                                                                                    cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                             <  1:3411670/150‑1 (MQ=255)

                                                                                                                        cGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTTCGCTTTTTGCCCAACATTAAGACGAAAATTTCCCGGGAATTGTGAAAAAATACTCGACAACGCGCAATAACCAtt                         >  1:2176134/1‑150 (MQ=255)

                                                                                                                         ggCGTTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGTTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtc                        <  1:734184/150‑1 (MQ=255)

                                                                                                                          gcgcTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                       >  2:2454270/1‑150 (MQ=255)

                                                                                                                           cgcTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                      <  2:120979/150‑1 (MQ=255)

                                                                                                                            gcTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACTCGACAGCGCGCAATAACCGTTCTCg                     >  1:3803889/1‑150 (MQ=255)

                                                                                                                            gcTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                     >  2:310213/1‑150 (MQ=255)

                                                                                                                            gcTTTTCCAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCg                     >  2:2821832/1‑150 (MQ=255)

                                                                                                                                   cAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa              <  1:3057530/150‑1 (MQ=255)

                                                                                                                                   cAGTATGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa              <  2:1428665/150‑1 (MQ=255)

                                                                                                                                       aTGTTGCTAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg          >  2:173700/1‑150 (MQ=255)

                                                                                                                                              tAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc   <  1:2161912/150‑1 (MQ=255)

                                                                                                                                              tAAAAATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc   >  2:3456906/1‑150 (MQ=255)

                                                                                                                                               aaaaATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg  <  2:2567729/150‑1 (MQ=255)

CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATG‑AAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450144‑450436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGC  >  CP009273/450141‑450437

GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                     >  SRR19973061.3553030/1‑150 (MQ=60)

   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                  >  SRR19973061.849000/1‑150 (MQ=60)

     TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTAGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTT                                                                                                                                                >  SRR19973061.1467403/1‑150 (MQ=60)

      GCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTA                                                                                                                                               <  SRR19973061.2661240/150‑1 (MQ=60)

       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                              >  SRR19973061.1654861/1‑150 (MQ=60)

       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                              <  SRR19973061.3308034/150‑1 (MQ=60)

       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                              <  SRR19973061.1437431/150‑1 (MQ=60)

        GCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG                                                                                                                                             <  SRR19973061.2360007/150‑1 (MQ=60)

          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                           >  SRR19973061.3597660/1‑150 (MQ=60)

          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                           >  SRR19973061.3597732/1‑150 (MQ=60)

          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                           >  SRR19973061.3804995/1‑150 (MQ=60)

           GGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAA                                                                                                                                          >  SRR19973061.1113001/1‑150 (MQ=60)

               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                      <  SRR19973061.3108589/150‑1 (MQ=60)

               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                      <  SRR19973061.305818/150‑1 (MQ=60)

               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                      >  SRR19973061.1757203/1‑150 (MQ=60)

                 GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                    <  SRR19973061.1223518/150‑1 (MQ=60)

                 GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                    <  SRR19973061.3545484/150‑1 (MQ=60)

                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  <  SRR19973061.2161482/150‑1 (MQ=60)

                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  >  SRR19973061.711537/1‑150 (MQ=60)

                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  <  SRR19973061.1271323/150‑1 (MQ=60)

                        TACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGG                                                                                                                             <  SRR19973061.2959933/150‑1 (MQ=60)

                         ACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGC                                                                                                                            <  SRR19973061.149514/150‑1 (MQ=60)

                           ACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCG                                                                                                                          >  SRR19973061.3780843/1‑150 (MQ=60)

                           ACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCACGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCG                                                                                                                          >  SRR19973061.1824783/1‑150 (MQ=60)

                            CTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT                                                                                                                         >  SRR19973061.2691091/1‑150 (MQ=60)

                            CTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTACGAAAAACGGCCTGCGGGCCGT                                                                                                                         >  SRR19973061.2691090/1‑150 (MQ=60)

                             tcttccgatcTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                        <  SRR19973061.2237725/140‑1 (MQ=60)

                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                        >  SRR19973061.3519676/1‑150 (MQ=60)

                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                        <  SRR19973061.3076430/150‑1 (MQ=60)

                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                       >  SRR19973061.1051919/1‑150 (MQ=60)

                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                       >  SRR19973061.2024106/1‑150 (MQ=60)

                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                       >  SRR19973061.1485174/1‑150 (MQ=60)

                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                       >  SRR19973061.805646/1‑150 (MQ=60)

                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                       >  SRR19973061.1485946/1‑150 (MQ=60)

                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG                                                                                                                     >  SRR19973061.2018011/1‑150 (MQ=60)

                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG                                                                                                                     >  SRR19973061.3626189/1‑150 (MQ=60)

                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGACTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG                                                                                                                     <  SRR19973061.3106882/150‑1 (MQ=60)

                                    TAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                 >  SRR19973061.3668820/1‑150 (MQ=60)

                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                 >  SRR19973061.1807219/1‑150 (MQ=60)

                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                 <  SRR19973061.1163642/150‑1 (MQ=60)

                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                 <  SRR19973061.3068364/150‑1 (MQ=60)

                                     AGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTG                                                                                                                <  SRR19973061.3685993/150‑1 (MQ=60)

                                       TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTC                                                                                                              <  SRR19973061.2176009/150‑1 (MQ=60)

                                                  GCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCG                                                                                                   <  SRR19973061.1554307/150‑1 (MQ=60)

                                                  GCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCG                                                                                                   <  SRR19973061.649164/150‑1 (MQ=60)

                                                  GCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCG                                                                                                   <  SRR19973061.648558/150‑1 (MQ=60)

                                                  GCAGGACACCGTCTTTGCCTATACTCCCTGTCGTGGAGCAGGAATCATAGAGTAAAAACGCATTTGCTACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCG                                                                                                   <  SRR19973061.1909208/150‑1 (MQ=60)

                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTTCGC                                                                                                  >  SRR19973061.188807/1‑150 (MQ=60)

                                                    AGGACACCGTCTTTGCCTCTCCTTCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCT                                                                                                 >  SRR19973061.2450044/1‑150 (MQ=60)

                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttatggatttttagat                                                                                                 >  SRR19973061.2286843/1‑126 (MQ=60)

                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCT                                                                                                 >  SRR19973061.2402129/1‑150 (MQ=60)

                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGAGCT                                                                                                 >  SRR19973061.694159/1‑150 (MQ=60)

                                                      GACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTT                                                                                               <  SRR19973061.1391504/150‑1 (MQ=60)

                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTT                                                                                              <  SRR19973061.305108/150‑1 (MQ=60)

                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                             >  SRR19973061.121375/1‑150 (MQ=60)

                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                            >  SRR19973061.473743/1‑150 (MQ=60)

                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                            <  SRR19973061.182994/150‑1 (MQ=60)

                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTG                                                                                            >  SRR19973061.473776/1‑150 (MQ=60)

                                                           CGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTGCGATTTTTGCC                                                                                          >  SRR19973061.3629350/1‑150 (MQ=60)

                                                               TTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTGAGCTTTTTGACCAGC                                                                                      >  SRR19973061.1355429/1‑150 (MQ=60)

                                                                 TGCCTCTCCTCCCTGTCGTTTAGTAGGAAGCATCGCGTAAAAACGAATTTGCTAATGTCGGCTCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                    <  SRR19973061.499339/150‑1 (MQ=60)

                                                                 TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGACCAGCAT                                                                                    >  SRR19973061.2014484/1‑150 (MQ=60)

                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTC                                                                                  <  SRR19973061.2396243/150‑1 (MQ=60)

                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTC                                                                                  >  SRR19973061.638460/1‑150 (MQ=60)

                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA                                                                                 <  SRR19973061.2547917/150‑1 (MQ=60)

                                                                     TCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAG                                                                                <  SRR19973061.493495/150‑1 (MQ=60)

                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAC                                                                              >  SRR19973061.615034/1‑150 (MQ=60)

                                                                          TCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAA                                                                           <  SRR19973061.2332068/150‑1 (MQ=60)

                                                                            CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAA                                                                         <  SRR19973061.587407/150‑1 (MQ=60)

                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAT                                                                        <  SRR19973061.3619627/150‑1 (MQ=60)

                                                                              TGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATT                                                                       <  SRR19973061.2478691/150‑1 (MQ=60)

                                                                               GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTATGGATTTTTCGCTTTTTGCCCAGCAATAAGACGAAAATTT                                                                      >  SRR19973061.132193/1‑150 (MQ=60)

                                                                                   TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGCGCTTTTTGCCCAGCATTaaaacgaaaaattaccg                                                                  >  SRR19973061.1076084/1‑133 (MQ=60)

                                                                                   TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGAGCTTTTTTCCCAGCATTAAGACGAAAATTGCCCG                                                                  >  SRR19973061.1797064/1‑150 (MQ=60)

                                                                                       GCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAA                                                              <  SRR19973061.2068296/150‑1 (MQ=60)

                                                                                       GCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAA                                                              <  SRR19973061.424431/150‑1 (MQ=60)

                                                                                           GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAACATTCAGACGAAAATTGCCCcggactttt                                                          >  SRR19973061.3000280/1‑141 (MQ=60)

                                                                                              taatctctTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAA                                                       <  SRR19973061.1730215/142‑1 (MQ=60)

                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGAGCTTTTTGCCCAGCATTCAAACGAAAATTGCCCGGGAATTGTGAAAA                                                     >  SRR19973061.1710515/1‑150 (MQ=60)

                                                                                                   GCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAT                                                  <  SRR19973061.3388065/150‑1 (MQ=60)

                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAC                                                <  SRR19973061.2812047/150‑1 (MQ=60)

                                                                                                      TAAAAACGCATTTGCATCTGTTGGCGATTTTCTAGTATGTTGCTAAATATTTTATGAAAAATGGTGTGCGGGCATTTTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCATTCAGAAGAAAATTGCCCGGGATTTGTGAAAAAATACG                                               <  SRR19973061.3698363/150‑1 (MQ=60)

                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACG                                               <  SRR19973061.1305764/150‑1 (MQ=60)

                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGC                                              >  SRR19973061.3109696/1‑150 (MQ=60)

                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGC                                              >  SRR19973061.629228/1‑150 (MQ=60)

                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGC                                              <  SRR19973061.2758334/150‑1 (MQ=60)

                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTCAGACAAAAATTGACCGGGAATTGTGAAAAAATAAGC                                              >  SRR19973061.3546656/1‑150 (MQ=60)

                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCG                                             <  SRR19973061.545288/150‑1 (MQ=60)

                                                                                                         AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGA                                            <  SRR19973061.1496470/150‑1 (MQ=60)

                                                                                                         AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAATGTGAAAAAATACGCGA                                            >  SRR19973061.3723552/1‑150 (MQ=60)

                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGAC                                           >  SRR19973061.3458937/1‑150 (MQ=60)

                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTAAGACGAAAATTGCCCGGGACTTGTGAAAAAATAAGCGAC                                           >  SRR19973061.3458934/1‑150 (MQ=60)

                                                                                                            CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAG                                         <  SRR19973061.487121/150‑1 (MQ=60)

                                                                                                            CGCATGTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAG                                         <  SRR19973061.2921583/150‑1 (MQ=60)

                                                                                                               ATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAAATGCCCGGGAATTGTGAAAAAATAAGCGACAACGC                                      >  SRR19973061.2715073/1‑150 (MQ=60)

                                                                                                                  TGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCA                                   >  SRR19973061.1252920/1‑150 (MQ=60)

                                                                                                                    CAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTTCGCTTTTTTCCCAGCAATCAAACAAAAAATTCCCGGGAATTGTGAAAAAATAaacaacagcgcgccaa                                 >  SRR19973061.2563703/1‑134 (MQ=60)

                                                                                                                      ACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttattgatttttagcttttttaccaacaataaaacaaaaaatgcccgggaatttttaaaaaataaacaacaacacacaaaaa                               >  SRR19973061.3240790/1‑68 (MQ=60)

                                                                                                                      ACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTATGGATTTTGCGCTTTTTTACCAGCAATAAAACGAAAAATTCCCGGGAAATGTGAAAAAATAAACGACAACGCGCAAAAA                               >  SRR19973061.1703585/1‑150 (MQ=60)

                                                                                                                       CTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAC                              <  SRR19973061.3420431/150‑1 (MQ=60)

                                                                                                                        TGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTtttttttattgattttgggcttttttaccaacaataagacgaaaaattccccggaaatttgaaaaaataaacaaaaaagcgcaataacc                             >  SRR19973061.987732/1‑61 (MQ=60)

                                                                                                                           CGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTTCGCTTTTTGCCCAACATTAAGACGAAAATTTCCCGGGAATTGTGAAAAAATACTCGACAACGCGCAATAACCATT                          >  SRR19973061.2181604/1‑150 (MQ=60)

                                                                                                                            GGCGTTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGTTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTC                         <  SRR19973061.736107/150‑1 (MQ=60)

                                                                                                                            GGCGATTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTC                         <  SRR19973061.2929774/150‑1 (MQ=60)

                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACTCGACAGCGCGCAATAACCGTTCTCG                      >  SRR19973061.3813582/1‑150 (MQ=60)

                                                                                                                                 TTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTATTGATTTTGCGCTTTTTTCCCAACAATCAAACGAAAATTTCCCCGGAATTGTGAAAATATACGCGACAGCGCGCAAAAACCGTTCTCGAC                    >  SRR19973061.2719279/1‑150 (MQ=60)

                                                                                                                                 TTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAC                    <  SRR19973061.3406322/150‑1 (MQ=60)

                                                                                                                                  TTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCACCATTCAGACGAAAATTGCCCAGGAATTGTGAAAAAATAaacgacaacgcgcaaaaaccaatatcgaat                   >  SRR19973061.2289748/1‑120 (MQ=60)

                                                                                                                                    TCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTATGGATTTTtaacttttttcccagccaaaagacaaaaaatacccgggaattttgaaaaaataaacgacaacccgcaaaaaacattaacaactaa                 >  SRR19973061.1067176/1‑65 (MQ=60)

                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGTCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATA               <  SRR19973061.3065432/150‑1 (MQ=60)

                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATTTCCCGGGAATTGTGAAAAAATACACGACAGCGCGCAATAACCGTTATCGACTCATA               >  SRR19973061.3117530/1‑150 (MQ=60)

                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATA               <  SRR19973061.3065429/150‑1 (MQ=60)

                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATA               <  SRR19973061.3065425/150‑1 (MQ=60)

                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAATTCCCGGGAATTTTGAAAAAATACACGACAACGCGCAATAACCGTTATCTACTCATAA              >  SRR19973061.3725311/1‑150 (MQ=60)

                                                                                                                                       AGTATGTTGCTAAAAAGTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGACTCATAA              >  SRR19973061.1257599/1‑150 (MQ=60)

                                                                                                                                        GTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCAATCAGACGAAAATTGCCCGGGAAATGTGAAAAAATACACGACAGCGCGCAAAAACCATTCACGACTCATAAA             >  SRR19973061.3561656/1‑150 (MQ=60)

                                                                                                                                          ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAG           <  SRR19973061.3712417/150‑1 (MQ=60)

                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGtaatttgagcatttttaacaacataaataagaaaattacccaggaatagttaaaaaataaacaaaagcacacaaaaaaaattataaaataaaaaacttgat       >  SRR19973061.1964295/1‑49 (MQ=60)

                                                                                                                                                 TAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC    <  SRR19973061.2167340/150‑1 (MQ=60)

                                                                                                                                                   AAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTGTGGATTTTGCGCTTTTTTCCCAGCCTTCAAACGAAAATTTCCCGGGAAATTTGAAAAAATACGCGACAACGCGCAATAAACAATATCGACTCATAAAAATGATGACGC  >  SRR19973061.2976763/1‑150 (MQ=60)

GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGC  >  CP009273/450141‑450437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`