Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 617.9 / ‑6.7 189intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (90/98);  minor base T (0/1);  total (90/99)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 191 total aligned reads displayed.

ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG  >  CP009273/450145‑450433
                                                                                                                                                 |                                                                                                                                                 
aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCGCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTTAatat                                                                                                                                                 <  1:1314850/148‑1 (MQ=255)
 tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                <  2:3768426/148‑1 (MQ=255)
 tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                <  2:3768750/148‑1 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                              <  1:1924652/148‑1 (MQ=255)
     cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                            <  2:974463/148‑1 (MQ=255)
     cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                            >  2:3221448/1‑148 (MQ=255)
     cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                            >  2:3547276/1‑148 (MQ=255)
      tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATg                                                                                                                                           <  2:798000/148‑1 (MQ=255)
       ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                          >  1:452451/1‑148 (MQ=255)
        gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                         >  1:2777385/1‑148 (MQ=255)
        gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                         >  2:2851640/1‑148 (MQ=255)
         ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaa                                                                                                                                        >  2:2696551/1‑148 (MQ=255)
           ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                      >  1:670838/1‑148 (MQ=255)
               aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                  <  2:1484940/148‑1 (MQ=255)
               aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                  <  2:318666/148‑1 (MQ=255)
                 aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                >  2:1522900/1‑148 (MQ=255)
                   ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                              >  2:447061/1‑148 (MQ=255)
                   ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                              >  1:3606109/1‑148 (MQ=255)
                    tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                             >  2:2596289/1‑148 (MQ=255)
                    tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                             >  2:2596245/1‑148 (MQ=255)
                      cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCggg                                                                                                                           >  1:2863956/1‑148 (MQ=255)
                       acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                          <  2:2662962/148‑1 (MQ=255)
                       acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                          <  2:3744316/148‑1 (MQ=255)
                       acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                          >  2:1960874/1‑148 (MQ=255)
                          aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt                                                                                                                       >  1:317087/1‑148 (MQ=255)
                           ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtg                                                                                                                      >  1:3533181/1‑147 (MQ=255)
                           ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑t                                                                                                                     <  2:849366/148‑1 (MQ=255)
                           ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑t                                                                                                                     <  2:2765124/148‑1 (MQ=255)
                             aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTGGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑ttt                                                                                                                   <  1:1468735/148‑1 (MQ=255)
                              aGGTGTGGGTAGGGTTGCAGGACACCGTGTTTGCCTCTCCTCCCTGTGTTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCgt‑tttg                                                                                                                  <  1:2654052/148‑1 (MQ=255)
                              aGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttg                                                                                                                  >  2:3539081/1‑148 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgt                                                                                                                 >  1:3052437/1‑148 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑gttgt                                                                                                                 >  2:2198987/1‑148 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑gttgt                                                                                                                 >  1:2452477/1‑148 (MQ=255)
                                 aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgttt                                                                                                               <  2:818836/148‑1 (MQ=255)
                                    gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑tttgttttgt                                                                                                            <  1:3128111/148‑1 (MQ=255)
                                    gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑tttgttttgt                                                                                                            <  2:122022/148‑1 (MQ=255)
                                     ggACGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑c                                                                                                          <  1:1388385/148‑1 (MQ=255)
                                      gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑gttgttttgtgt                                                                                                          >  1:1565152/1‑146 (MQ=255)
                                      gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑Ct                                                                                                         >  2:2512199/1‑148 (MQ=255)
                                       aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑gttgttttttgtg                                                                                                         >  1:1917693/1‑143 (MQ=255)
                                        aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTgg                                                                                                       <  2:1413993/148‑1 (MQ=255)
                                          ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGAt                                                                                                     <  2:2298190/148‑1 (MQ=255)
                                          ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGGTTTGT‑GTgggt                                                                                                     >  1:1705500/1‑146 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGTAGCAGTAAGGATGGCGTAAAAACGAATTTTCAACTTTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTTTGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGAttt                                                                                                   <  1:2659511/148‑1 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGATTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGAttt                                                                                                   <  1:3700528/148‑1 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGAttt                                                                                                   <  1:2937512/148‑1 (MQ=255)
                                                aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTgcg                                                                                               <  2:3434324/148‑1 (MQ=255)
                                                 ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTgcgc                                                                                              <  1:746130/148‑1 (MQ=255)
                                                  gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCt                                                                                             <  1:2288468/148‑1 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCttt                                                                                           <  1:503308/148‑1 (MQ=255)
                                                      ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑TTTGTTTTTT‑GTGGTTTTTTGGGttttt                                                                                         >  1:151296/1‑148 (MQ=255)
                                                        gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGc                                                                                       <  1:1033860/148‑1 (MQ=255)
                                                        gTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGTAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGc                                                                                       <  1:1034325/148‑1 (MQ=255)
                                                          cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGGTTTGT‑GTGGATTTTGCGCTTTTTTccc                                                                                     >  1:964010/1‑148 (MQ=255)
                                                          cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTGGTTTTTTGTTGGTTTTTGGGTTTTTTGcc                                                                                      >  1:1169022/1‑148 (MQ=255)
                                                            ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAg                                                                                   >  2:1900884/1‑148 (MQ=255)
                                                              gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCa                                                                                 <  1:2746939/148‑1 (MQ=255)
                                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCAtt                                                                               <  2:3755073/148‑1 (MQ=255)
                                                                 tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTTCGCTTTTTGCCCAGCAATc                                                                              >  2:2943094/1‑148 (MQ=255)
                                                                       cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTT‑CTGGATTTTGCGCTTTTTTCCCCGCATTCAGACGa                                                                        >  2:1787207/1‑148 (MQ=255)
                                                                        ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTT‑CTGGATTTTTGGCTTTTTGCCCAGCATTAAGACGaa                                                                       >  2:1817848/1‑148 (MQ=255)
                                                                         cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                      <  1:795215/148‑1 (MQ=255)
                                                                          tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaaa                                                                     <  1:235365/148‑1 (MQ=255)
                                                                            tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAtt                                                                   <  1:3796128/148‑1 (MQ=255)
                                                                               tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTT‑GTGGGTTTTTGGGTTTTTTGCCAGCAATCAGACGaaaaattgc                                                                >  1:2393114/1‑143 (MQ=255)
                                                                                  aGCAGGAAGAATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                                             <  1:425830/148‑1 (MQ=255)
                                                                                     aGGAAGCATCGCGTAAAAACGCATGTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGaa                                                          <  2:2446968/148‑1 (MQ=255)
                                                                                      ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTT‑GTGGGTTTTGGGGTTTTTTGCCAGCATTCAGACGAAAAATGCCCggggca                                                         >  1:3437207/1‑145 (MQ=255)
                                                                                      ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGGTTTTT‑GTGGGTTTTGCGCTTTTTTGCCCGCAATCAGACGAAAAATGCCCgggcct                                                         >  1:3678501/1‑145 (MQ=255)
                                                                                      ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTGT‑GTGGTTTTTTGGGTTTTTTGCCAGCATTGAGACGAAAATTGCCCGGGGAt                                                         >  1:3437234/1‑148 (MQ=255)
                                                                                       gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTTTTTTTTT‑TGGGTTTTTGGGGTTTTTTGCCGCCATCAAGCGCAAAATTGCCCgggcgt                                                         >  1:30133/1‑145 (MQ=255)
                                                                                        aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                       <  1:1867434/148‑1 (MQ=255)
                                                                                        aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                       <  2:1479715/148‑1 (MQ=255)
                                                                                             tCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑TTTGTTTTGT‑GTGGATTTTGCGCTTTTTTCCCAGCATTCAGACCCAAAATTCCCGGGGATTGTGtaa                                                  >  2:3653856/1‑148 (MQ=255)
                                                                                                 gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                              <  1:1815458/148‑1 (MQ=255)
                                                                                                  tAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATa                                             <  1:2014490/148‑1 (MQ=255)
                                                                                                   aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑GTTGTTTTGT‑GTGGATTTTGCGCGTTGTGCCCCGCAATGAGCCGACACTTGCCCGGGCCTTGGGAAAAAATaa                                            >  2:2602632/1‑147 (MQ=255)
                                                                                                    aaaaCGCATTTGCAACTGTGGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcg                                           <  1:2563342/148‑1 (MQ=255)
                                                                                                    aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcg                                           <  1:3653856/148‑1 (MQ=255)
                                                                                                      aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                         <  2:611991/148‑1 (MQ=255)
                                                                                                      aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGGTTTTT‑CTGGGTTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                         >  2:867815/1‑148 (MQ=255)
                                                                                                        cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTT‑GTGGTTTTTTGGCTTTTTTGCCACCCTTCAGACGAAAAATTCCCGGGACATGTGTAAAAATaaccgcc                                       >  2:2922787/1‑142 (MQ=255)
                                                                                                        cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑TTTGTTTTTT‑GTGGGTTTTGCGCGTTTTGCCCCGCCTTGAGACGAAAATTGCCCGGGCATTGTGAAAAAATAAGCGAc                                       >  2:2354952/1‑148 (MQ=255)
                                                                                                            tttGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcg                                   <  1:2217154/148‑1 (MQ=255)
                                                                                                             ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGT‑CTGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                  >  2:1555337/1‑148 (MQ=255)
                                                                                                                   cTGTCGGCTCTTTTCCTGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGGTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATa                            <  2:573464/148‑1 (MQ=255)
                                                                                                                    tgtgGGGGCTTTCCCTGTTTTTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                           <  1:2246589/148‑1 (MQ=255)
                                                                                                                    tgtgGGCGTTTTTCCATTTTGTTCATAAAATTTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                           <  1:3221448/148‑1 (MQ=255)
                                                                                                                     gTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                          <  2:2920402/148‑1 (MQ=255)
                                                                                                                          cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTTTTTTTT‑TTTGATTTTGAGCTTTTTTGCCCGCAATAAGAAGAAAATTTCCCGGGAATTGTGTAAAAATAAACCCCAGCCCGCAaaaaaacgta                     >  1:2059871/1‑142 (MQ=255)
                                                                                                                                tCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt               <  2:342043/148‑1 (MQ=255)
                                                                                                                                    gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTGT‑CTGGCTTTTGGGCTTTTTGCCCAGCATTCAGTCGAAAATTGCCCGGGCCTTGTGAAAAAATACGCGCCCGCGCGCAATAACCGTTGTCGACTCATa           >  2:3111111/1‑148 (MQ=255)
                                                                                                                                     tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTT‑CTTGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTTAAAAAATAAGCGACAGCGCGCAAAAAACGTTCTAGACTCATaa          >  2:2045646/1‑148 (MQ=255)
                                                                                                                                      aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTT‑CTGGATTTTTCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAAACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa         >  2:3364962/1‑148 (MQ=255)
                                                                                                                                      aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa         <  2:3334358/148‑1 (MQ=255)
                                                                                                                                        gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑GTTGATTTTTCGCTTTTTGCCCAGCATTCAGAAGAAAAATTCCCGGGAATTTTTTAAAAATACGCGACAGCGCGCAATAAACGTTCTCGACTCATaaaat       >  2:512896/1‑147 (MQ=255)
                                                                                                                                        gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑GTGGATTTTTGGCTTTTTGCCCACCATTCAGACGAAAATTTCCCGGGAATTTTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACTCATaaaat       >  2:2601393/1‑147 (MQ=255)
                                                                                                                                             tAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg  <  2:670838/148‑1 (MQ=255)
                                                                                                                                             tAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg  <  2:1269827/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGT‑CTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG  >  CP009273/450145‑450433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450141‑450434
                                                                                                                                                     |                                                                                                                                                  
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAA                                                                                                                                                    >  SRR19973069.2947647/1‑150 (MQ=60)
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                    >  SRR19973069.1600337/1‑150 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                   >  SRR19973069.3356520/1‑150 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                   >  SRR19973069.3356200/1‑150 (MQ=60)
    ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCGCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTTAATATTT                                                                                                                                                <  SRR19973069.1323858/150‑1 (MQ=60)
    ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTT                                                                                                                                                <  SRR19973069.1323920/150‑1 (MQ=60)
     TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTT                                                                                                                                               <  SRR19973069.3195646/150‑1 (MQ=60)
       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                             <  SRR19973069.1937883/150‑1 (MQ=60)
        GCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATG                                                                                                                                            <  SRR19973069.3038745/150‑1 (MQ=60)
         CTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGA                                                                                                                                           >  SRR19973069.455759/1‑150 (MQ=60)
          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                          >  SRR19973069.2796062/1‑150 (MQ=60)
          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                          >  SRR19973069.866768/1‑150 (MQ=60)
          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                          >  SRR19973069.2795884/1‑150 (MQ=60)
             CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                       >  SRR19973069.1278365/1‑150 (MQ=60)
             CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                       >  SRR19973069.675444/1‑150 (MQ=60)
                TGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGG                                                                                                                                    >  SRR19973069.2602779/1‑150 (MQ=60)
                 GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                   <  SRR19973069.2339550/150‑1 (MQ=60)
                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                 >  SRR19973069.2393669/1‑150 (MQ=60)
                     ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                               >  SRR19973069.2779227/1‑150 (MQ=60)
                     ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                               >  SRR19973069.3629653/1‑150 (MQ=60)
                        TACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGG                                                                                                                            >  SRR19973069.2882776/1‑150 (MQ=60)
                            CTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT                                                                                                                        >  SRR19973069.319177/1‑150 (MQ=60)
                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTG                                                                                                                       >  SRR19973069.3556075/1‑150 (MQ=60)
                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgg                                                                                                                       >  SRR19973069.3556089/1‑148 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG                                                                                                                    <  SRR19973069.1458288/150‑1 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGT                                                                                                                   >  SRR19973069.3072635/1‑150 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGT                                                                                                                   >  SRR19973069.2468971/1‑150 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGGTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggggg                                                                                                                   >  SRR19973069.3651703/1‑145 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTGGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGT                                                                                                                   <  SRR19973069.1478978/150‑1 (MQ=60)
                                  AGGTGTGGGTAGGGTTGCAGGACACCGTGTTTGCCTCTCCTCCCTGTGTTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTT                                                                                                                  <  SRR19973069.2671837/150‑1 (MQ=60)
                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGACGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                <  SRR19973069.3137972/150‑1 (MQ=60)
                                     AGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTT                                                                                                               >  SRR19973069.2227541/1‑150 (MQ=60)
                                        GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT                                                                                                            <  SRR19973069.3148634/150‑1 (MQ=60)
                                        GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT                                                                                                            <  SRR19973069.189056/150‑1 (MQ=60)
                                        GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGT                                                                                                            >  SRR19973069.1575826/1‑150 (MQ=60)
                                         GGACGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTG                                                                                                           <  SRR19973069.1398070/150‑1 (MQ=60)
                                         GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtg                                                                                                           >  SRR19973069.1930899/1‑145 (MQ=60)
                                            AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgtgttttgtgggg                                                                                                        >  SRR19973069.692580/1‑133 (MQ=60)
                                            AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttgttgtt                                                                                                        >  SRR19973069.693059/1‑134 (MQ=60)
                                            AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAT                                                                                                        <  SRR19973069.1061347/150‑1 (MQ=60)
                                            AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTGTGTGGGT                                                                                                        >  SRR19973069.1717438/1‑150 (MQ=60)
                                             GGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATT                                                                                                       <  SRR19973069.455091/150‑1 (MQ=60)
                                              GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttttgtggtttt                                                                                                      >  SRR19973069.14392/1‑131 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGTAGCAGTAAGGATGGCGTAAAAACGAATTTTCAACTTTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTTTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTG                                                                                                    <  SRR19973069.2677337/150‑1 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGATTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTG                                                                                                    <  SRR19973069.3724714/150‑1 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTG                                                                                                    <  SRR19973069.2956993/150‑1 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTG                                                                                                    <  SRR19973069.3724708/150‑1 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTG                                                                                                    <  SRR19973069.2679422/150‑1 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTG                                                                                                    <  SRR19973069.2956882/150‑1 (MQ=60)
                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgttttttgtgggttttgggg                                                                                                 >  SRR19973069.770025/1‑126 (MQ=60)
                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtgggtttttggg                                                                                                 >  SRR19973069.1539738/1‑137 (MQ=60)
                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATGAAAAACGGCCAGCGGGCCGTTTTGTTTTGTgtgggtatttcggt                                                                                                >  SRR19973069.3118621/1‑136 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTT                                                                                               <  SRR19973069.3838316/150‑1 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGATTTTCCAGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTT                                                                                               <  SRR19973069.751417/150‑1 (MQ=60)
                                                      GACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTT                                                                                              <  SRR19973069.2304017/150‑1 (MQ=60)
                                                      GACACCGTCTTTGCCACTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTT                                                                                              <  SRR19973069.2303308/150‑1 (MQ=60)
                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttggttttttgggtgttttggggttt                                                                                             >  SRR19973069.110476/1‑123 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                            <  SRR19973069.2441303/150‑1 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTTGTTTTttgtggttttttgggttttt                                                                                            >  SRR19973069.152634/1‑130 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACACTATGTTTCTAAAAATTTTATTAAAAAAGGCCTGagggcgggggtgttttgtgtgtgtttggtgtatttt                                                                                            >  SRR19973069.153262/1‑114 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                            <  SRR19973069.506916/150‑1 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtggttttttgttggtttttgggttttttgcc                                                                                        >  SRR19973069.1177134/1‑118 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTGTGTGGATTTTGCGCTTTTTTCCC                                                                                        >  SRR19973069.970443/1‑150 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCC                                                                                        <  SRR19973069.1041028/150‑1 (MQ=60)
                                                            GTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGTAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCC                                                                                        <  SRR19973069.1041499/150‑1 (MQ=60)
                                                                  GCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtttttttttttggtttttgggtttttttgcaaccat                                                                                  >  SRR19973069.1671020/1‑112 (MQ=60)
                                                                  GCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT                                                                                  <  SRR19973069.2765293/150‑1 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtggtttttggggttttttggcagcaata                                                                                 >  SRR19973069.2113765/1‑121 (MQ=60)
                                                                      CTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtggtgttttggcttttttgcccgccatacgg                                                                              >  SRR19973069.1562334/1‑116 (MQ=60)
                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttgttttgggttttggggttttttgcgccaataacaacaaaaa                                                                       >  SRR19973069.1955691/1‑100 (MQ=60)
                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttggtttttggggggtttggggttttttgccagccagaacacgtaaaa                                                                       >  SRR19973069.2412227/1‑101 (MQ=60)
                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttggtgtgtttggggttttttgccaccattaagacgcaaaa                                                                       >  SRR19973069.2412999/1‑101 (MQ=60)
                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAAT                                                                      <  SRR19973069.800854/150‑1 (MQ=60)
                                                                              TGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATT                                                                     <  SRR19973069.237149/150‑1 (MQ=60)
                                                                                TCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC                                                                   <  SRR19973069.3820715/150‑1 (MQ=60)
                                                                                TCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGC                                                                   <  SRR19973069.3820726/150‑1 (MQ=60)
                                                                                 CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttgggttttttgccagcaatcagacgaaaaattgc                                                                   >  SRR19973069.2409328/1‑105 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGgcggttttgttttttttgggttttttgtgtttgtgcccccggcataaccaaattatgcag                                                                >  SRR19973069.976006/1‑90 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggggggtttttttggggtttgggggttttggccccgataagcacaaaatatgcccg                                                                >  SRR19973069.976220/1‑93 (MQ=60)
                                                                                      AGCAGGAAGAATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGA                                                             <  SRR19973069.428953/150‑1 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtttttttggggtttttgggttttttgcccgccatcagacggaaaattcccggggct                                                            >  SRR19973069.3702559/1‑90 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGTGGGTTTTGGGGTTTTTTGCCAGCATTCAGACG‑AAAAATGCCCGGGgca                                                           >  SRR19973069.3459533/1‑147 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTTTGTGGGTTTTGCGCTTTTTTGCCCGCAATCAGACG‑AAAAATGCCCGGGcct                                                           >  SRR19973069.3702561/1‑147 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTGTGTGGTTTTTTGGGTTTTTTGCCAGCATTGAGACG‑AAAATTGCCCGGGGAT                                                           >  SRR19973069.3459560/1‑150 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtgtttttgtgtggggttttggcgttttgggccgccgtgtgacggaaaatggccggggctt                                                           >  SRR19973069.2077372/1‑89 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttttttttttgggtttttggggttttttgccgccatcaagcgcaaaattgcccgggcgt                                                           >  SRR19973069.30563/1‑89 (MQ=60)
                                                                                          GGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGTggggttttttgtgggttttgggggtttttgccagcaataaggccaaaaaatgcccgggaatg                                                          >  SRR19973069.1507428/1‑88 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTGCAGTATGTTGGTAAAAATTTTATTAAAAAAGGCCTGCGGGCCGggtgtgtttttgtggggtttttgggttttgggcccccaacacaacaaacagtgccgcggagtgtg                                                        >  SRR19973069.1741882/1‑85 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTG                                                       <  SRR19973069.1880450/150‑1 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGTGTTGTTTTttttgggttttgtggttttttggcccccgaacgggccaaaatgagcggggagtggg                                                        >  SRR19973069.1743011/1‑94 (MQ=60)
                                                                                             AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGA                                                      <  SRR19973069.1761724/150‑1 (MQ=60)
                                                                                             AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttttgtgggtttttggggttttttgccgccgtgagccgcaaaattcgcggggcgtgtgt                                                       >  SRR19973069.1447415/1‑84 (MQ=60)
                                                                                             AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtgttttttttttgtttttttggttttttgcccgccctcaggagtaaaagtgcccgggaatgtgt                                                       >  SRR19973069.1700529/1‑85 (MQ=60)
                                                                                             AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttttgtggtgtttgggggtttttgcgccccgcaagaggacaaagtgggcggcgcggggg                                                       >  SRR19973069.1445256/1‑84 (MQ=60)
                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggtttttttttgtgggtttttggggtttttgccccccgtgagtcgcaaaattcccgggggctgtggtaa                                                    >  SRR19973069.143408/1‑81 (MQ=60)
                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgttggttttgggcgttttggccacccattagacgcaaaatggcccgggattgtgttaa                                                    >  SRR19973069.2443339/1‑92 (MQ=60)
                                                                                                  CGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgttgtttttttgggttttttggcccaataagacgaacatttgccgggggttttttgaaaa                                                  >  SRR19973069.3605580/1‑80 (MQ=60)
                                                                                                    CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggggggttttttttggttgttgggggttttgcgccccataagacgcaaaataacgggggcgggtgtgtaaaaa                                                >  SRR19973069.1317550/1‑77 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                              <  SRR19973069.1828210/150‑1 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                              <  SRR19973069.2012978/150‑1 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttgggttttttgccccccctaaaaccaaaaattaccggggcatgtgtaaaaaaaa                                               >  SRR19973069.214392/1‑85 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACG                                             <  SRR19973069.488532/150‑1 (MQ=60)
                                                                                                      TAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACG                                             <  SRR19973069.2028495/150‑1 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTGGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                           <  SRR19973069.2580271/150‑1 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                           <  SRR19973069.3677786/150‑1 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                           <  SRR19973069.2822378/150‑1 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                           <  SRR19973069.2580264/150‑1 (MQ=60)
                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttgttttttgggttttttgcccgcctaaagacgcaaaattgccggggcgtttttaaaaattatagcc                                          >  SRR19973069.2978744/1‑72 (MQ=60)
                                                                                                              CATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttgtggatttttcgggttttgcccaccaatcagaccaaaaattcccgggaaatttgtaaaaataatcgacagag                                      >  SRR19973069.3450399/1‑68 (MQ=60)
                                                                                                                TTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCG                                   <  SRR19973069.2232250/150‑1 (MQ=60)
                                                                                                                TTTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCG                                   <  SRR19973069.2232161/150‑1 (MQ=60)
                                                                                                                  TGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtgggtttttgggtttttttgccccaaagagaacaaaaaatacccgggcgtgtgtgaaaaaaaacgacaccgcgcc                                  >  SRR19973069.3665208/1‑64 (MQ=60)
                                                                                                                  TGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttggggttttttccccccatgacaagaaaaattgcccgggactgtgtaaaaaaaaacgacaccccggc                                  >  SRR19973069.3665185/1‑72 (MQ=60)
                                                                                                                            GGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttggtttttgggttttttgccaaccaaaagaacaaaaaagaccggggaatttttaaaaaaaaaagaaagcccccaaaaaaacaaa                        >  SRR19973069.2072061/1‑54 (MQ=60)
                                                                                                                            GGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTCTGGTTTTTTAGCTTTTTTCCCAGCAATCAGACG‑AAAAAAGCCCGGGAATTGTGAAAAAAaaaacgacaacccgcaaaaaaccttc                       >  SRR19973069.615756/1‑124 (MQ=60)
                                                                                                                            GGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTTTTGATTTTGAGCTTTTTTGCCCGCAATAAGAAG‑AAAATTTCCCGGGAATTGTGTAAAAATAaaccccagcccgcaaaaaaacgta                       >  SRR19973069.2074155/1‑126 (MQ=45)
                                                                                                                              CGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttgtgtggttttttgggttttttataaccactaaaaggcaaaaatgcccggcaaatttaaaaaaaaaaaaaacaaacccacaaaacaataaa                      >  SRR19973069.1656831/1‑52 (MQ=60)
                                                                                                                               GNTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTGTGTGGATTTTTGGCTTTTTTGCCAGCCATCAGACG‑AAAAATTCCCGGGAATTGTGAAAAAATAAGCGACCGCGCG‑CAAaaaaccgtctcg                   >  SRR19973069.3502071/1‑138 (MQ=60)
                                                                                                                                 TTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgttggtttttgggttttttaccaccaaacagacgaaaaaatacccggcatttttaaaaaaaaaacgacaacgcccaaaaaaacttcacgaa                   >  SRR19973069.1480603/1‑49 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgttgtttttttggtttttttcccgcagtaagacgaaaaattaccgcggattttttaaaaaaaaaccaaacagcgcccaaaaacgtatagccac                 >  SRR19973069.682232/1‑55 (MQ=60)
                                                                                                                                     CCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCAT             <  SRR19973069.1830608/150‑1 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtggttttttgggttttttgccagcaataagaacaaaaattcccggggaattttgaaaaataaacgaaaccgcgccaaaaaactggacggcacaaaa             >  SRR19973069.1039486/1‑51 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttattgttttttgggatttttaccagcaattacaagaaaaaatcacggggaatggttaaaaaaaaacgaaaccccgcaacaaacgttgaagaaacaaaa             >  SRR19973069.2328273/1‑51 (MQ=60)
                                                                                                                                                CTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGTTTTTTGGCTTTTTTGCCACCATTCAGACGAAAAATTGCCC‑GGAATTGTGTAAAAAAAAGCGACAGCGCGCCAAAAACCG‑TCTGGACTCAAAAAAGTGTTGC  >  SRR19973069.1246838/1‑150 (MQ=23)
                                                                                                                                                CTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGATTTTTGGGTTTTTTGCCAGCAATCAGACG‑AAAAATGGCCGGGCCATGTGAAAAAAAAAGCGACACCGCG‑CAAAAAACGTTCTCGACACATAAAAattattc  >  SRR19973069.3163040/1‑143 (MQ=29)
                                                                                                                                                     |                                                                                                                                                  
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCG‑CAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450141‑450434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑