Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA99.3% 1004.0 / ‑2.6 300intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (171/126);  total (173/127)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 302 total aligned reads displayed.

CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450144‑450435
                                                                                                                                                  |                                                                                                                                                      
cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt                                                                                                                                                      >  2:2381395/1‑149 (MQ=255)
 aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                     >  2:128418/1‑149 (MQ=255)
        ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                              <  1:4418613/149‑1 (MQ=255)
           tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                           <  1:1358895/149‑1 (MQ=255)
             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                         >  2:1236450/1‑149 (MQ=255)
             tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACg                                                                                                                                         >  2:2809499/1‑149 (MQ=255)
               cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                       <  2:296314/149‑1 (MQ=255)
                aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                      >  2:3072148/1‑149 (MQ=255)
                 tACTTACACTATTAAGTAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                     <  2:1955554/149‑1 (MQ=255)
                 tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                     >  2:3245587/1‑149 (MQ=255)
                 tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                     >  2:3673103/1‑149 (MQ=255)
                  aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTg                                                                                                                                    >  2:4280096/1‑149 (MQ=255)
                    ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                                  >  1:3765341/1‑149 (MQ=255)
                       cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                               <  2:1000917/149‑1 (MQ=255)
                       cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                               >  2:4115394/1‑149 (MQ=255)
                         cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCg                                                                                                                             >  1:3059539/1‑149 (MQ=255)
                           aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                           <  1:2460106/149‑1 (MQ=255)
                            ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                          >  2:4555130/1‑149 (MQ=255)
                             tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACACATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgggtt                                                                                                                         >  1:505909/1‑145 (MQ=255)
                              aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtggtt                                                                                                                        >  1:574411/1‑145 (MQ=255)
                                 gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑ttt                                                                                                                    <  1:3449037/149‑1 (MQ=255)
                                   gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttg‑ttttg                                                                                                                  >  2:3191570/1‑149 (MQ=255)
                                   gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttg‑ttttg                                                                                                                  >  2:2292317/1‑149 (MQ=255)
                                   gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGtgttg‑ttttg                                                                                                                  >  1:296271/1‑149 (MQ=255)
                                     gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑ttttttg                                                                                                                >  1:2249649/1‑146 (MQ=255)
                                     gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑c                                                                                                               <  2:4749524/149‑1 (MQ=255)
                                             ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑Atttt                                                                                                      <  1:182277/149‑1 (MQ=255)
                                             ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTTT‑GTGG‑Ttttt                                                                                                      >  1:3764201/1‑149 (MQ=255)
                                                 aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGgtg‑gttttt‑gtgg‑tttttttgg                                                                                                  >  1:1224554/1‑145 (MQ=255)
                                                 aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑GTGG‑Atttttggc                                                                                                  >  1:4691712/1‑145 (MQ=255)
                                                  ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCt                                                                                                 >  2:636453/1‑149 (MQ=255)
                                                  ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCt                                                                                                 <  2:1052359/149‑1 (MQ=255)
                                                  ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCt                                                                                                 <  1:2194668/149‑1 (MQ=255)
                                                    acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑ATTTTTCGCttt                                                                                               >  2:4336231/1‑149 (MQ=255)
                                                     cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCtttt                                                                                              <  2:2162125/149‑1 (MQ=255)
                                                      acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑ATTTTGCGCttttt                                                                                             >  2:1905955/1‑149 (MQ=255)
                                                      acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑ATTTTGAGCttttt                                                                                             >  2:3614457/1‑149 (MQ=255)
                                                        cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑CTGG‑ATTTTGCGCTTTTTgg                                                                                           >  2:45826/1‑148 (MQ=255)
                                                          tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGccc                                                                                         <  1:2089979/149‑1 (MQ=255)
                                                          tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGGTGT‑TTTTTGGGTTTTTTGcc                                                                                          >  1:1678037/1‑149 (MQ=255)
                                                          tCTTTGCCTCTCCTCCCTGTCGTGGAGAATTAAGCAGCGCGTAAAAACGCATTTGAAACTGTCGGCTCTTTTCCAGTATGTTTCTAAATATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGccc                                                                                         <  1:4097297/149‑1 (MQ=255)
                                                            tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGG‑CTGG‑AGTTTGCGCTTTTTTccccg                                                                                       >  1:1059186/1‑147 (MQ=255)
                                                               gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑GTTTTGCGCTTTTTGCCCAGCaa                                                                                    >  2:1439466/1‑148 (MQ=255)
                                                               gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑ATTTTTCGCTTTTTGCCCAGCAt                                                                                    >  2:1530144/1‑149 (MQ=255)
                                                               gCCTCTCCGCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTGGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCAt                                                                                    <  1:4001559/149‑1 (MQ=255)
                                                                  tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTTG‑ATTTTGCGCTTTTTGCCCCGCATTCa                                                                                 >  2:1051124/1‑149 (MQ=255)
                                                                    tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                               <  1:3730800/149‑1 (MQ=255)
                                                                        cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑TTTG‑GTTTTGGGGTTTTTTGCCACCAGTCaaaaaca                                                                           >  1:4584307/1‑143 (MQ=255)
                                                                         ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑aaa                                                                        <  1:2381395/149‑1 (MQ=255)
                                                                           ggTCGTGGAGCAGGACGCAGCGCGTAAAAACGCATGTGCAACTGTCGGCGCTTTTCCAGTATGTGGCGAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CGGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAAt                                                                      <  2:3440744/148‑1 (MQ=255)
                                                                                 ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCg                                                                <  2:2243378/149‑1 (MQ=255)
                                                                                  gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑GTGG‑TTTTTTGGGTTTTTTGCCACCAATCATG‑CG‑AAAATTGCCCgg                                                               >  1:3353739/1‑149 (MQ=255)
                                                                                    gCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑GTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCgggc                                                             >  2:4195553/1‑148 (MQ=255)
                                                                                      aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAAt                                                           <  2:610009/149‑1 (MQ=255)
                                                                                       ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑TTTTTTCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAAtt                                                          >  2:2175951/1‑149 (MQ=255)
                                                                                       ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAAtt                                                          <  2:2869336/149‑1 (MQ=255)
                                                                                        gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATtg                                                         <  1:1070368/149‑1 (MQ=255)
                                                                                          aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTT‑CTGG‑TTTTTTGGCTTTTTGGCCAACATTCAGA‑CG‑AAAATTTCCCGGGGAttttt                                                       >  2:1843961/1‑146 (MQ=255)
                                                                                          aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTTT‑GTGG‑TTTTTGGGGTTTTTGGCCACCAATCAGA‑CGAAAAATTACCCgggcttgtg                                                        >  1:3313055/1‑143 (MQ=255)
                                                                                          aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑GTGG‑GTTTTTGGCTTTTTTGCCCCCAATCAGA‑AC‑CAAAATGCCCCGGACGtgtg                                                       >  1:1365145/1‑149 (MQ=255)
                                                                                          aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTTG‑TTTTTTGGTGT‑GTTTTG‑GCTTTTTTGCCAGCCATAAGA‑CGAAAAATGGCCCGGGAAtgtg                                                        >  1:3313016/1‑146 (MQ=255)
                                                                                             aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTGTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGaaa                                                    <  2:4608178/149‑1 (MQ=255)
                                                                                                  gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTTCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATa                                               >  2:3552937/1‑149 (MQ=255)
                                                                                                  gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑GTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCAGTCAGA‑CG‑CAAATTGCCCGGGACATGTGAAAAAATa                                               >  2:4652179/1‑149 (MQ=255)
                                                                                                   tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATAc                                              <  2:3784540/149‑1 (MQ=255)
                                                                                                   tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑GTGG‑GTTTTGGGCTTTTTTACCCCCAAACAGACCA‑AAAATTGCCCGGGAA‑TGTGTAAAAATaa                                              >  1:682401/1‑148 (MQ=255)
                                                                                                   tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑ATTTTGTGCTTTTTGCCCAGCATTCAGA‑CC‑AAAATTTCCCGGGACTTGTGAAAAAATaa                                              >  2:2470210/1‑148 (MQ=255)
                                                                                                   tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTACTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑GTGG‑TGTTTGCGCTTTTTTCCCCGCAATAAGA‑CG‑AAAAATGCCCGGGGAGTGTGAAAAAATaa                                              >  1:2375194/1‑148 (MQ=255)
                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTTTT‑GTGG‑TTTTTTGGTTTTTTTGCCCCCATAAGAA‑GG‑AAAAATGCCCGGGAGTTGTTaaaaataaa                                              >  1:3593714/1‑145 (MQ=255)
                                                                                                    aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTGTG‑TTTTTT‑GTGGTTTTTTGGGGTTTTTG‑GCAGCAATCAGA‑CG‑CAAAATTCCCGGGGATTTTGTAAAAATaag                                             >  1:3257658/1‑147 (MQ=255)
                                                                                                      aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑CGGG‑ATTTTGCGGTTTTTTCCCCCCCATCAGA‑CC‑AAAAATGCCCGGGCAATGTGgaaaaaaaaccc                                           >  1:4308194/1‑143 (MQ=255)
                                                                                                       aaCGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                          <  1:1723732/149‑1 (MQ=255)
                                                                                                       aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                          <  1:1723864/149‑1 (MQ=255)
                                                                                                         cgaaTTTGAAAGTGTCGGCGCTTTTCCTTTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                                        <  1:40083/146‑1 (MQ=255)
                                                                                                         cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGT‑CTGG‑ATTTTGCGGTTTTTGCCCAGCAATCCTA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                                        >  2:910322/1‑149 (MQ=255)
                                                                                                           cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAATCGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACCgc                                      >  2:4320117/1‑149 (MQ=255)
                                                                                                           cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTT‑CTGG‑ATTTTTCGCTTTTTTCCCAGCATTCAGA‑CG‑AAAAATGCCCGGGACTTGTGAAAAAATACGCGACAgc                                      >  2:1929959/1‑149 (MQ=255)
                                                                                                             tttGCAAGTGTCGGGGCTTTTCCTGTTTGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGATTTGTGAAAAAATACGCGACAgcgc                                    <  1:4216126/149‑1 (MQ=255)
                                                                                                             tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTT‑CTGG‑ATTTTGCGCTTTTTTACCCGCAATCAGA‑CG‑AAAATTGCCCGGGGATTGTGAAAAAATAAGCGACAgcgc                                    >  2:2138320/1‑149 (MQ=255)
                                                                                                               tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑GTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTTTGAAAAAATAAGCGACAgcgcgc                                  >  2:175303/1‑149 (MQ=255)
                                                                                                                 cAACTGTCGGCGCTTTTCCTTTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                <  1:2852225/149‑1 (MQ=255)
                                                                                                                 cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                <  2:2712624/149‑1 (MQ=255)
                                                                                                                 cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTTT‑TTTTTT‑CTGG‑CTTTTTCGCTTTTTTCCCCGCATTCAGA‑CG‑AAAATTTCCCGGGCATAGTGTAAAAATACGCGAAAGCGCGCac                                >  2:2078476/1‑148 (MQ=255)
                                                                                                                      gTCGGCGCTTTGCCTGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAcc                           <  1:4647864/149‑1 (MQ=255)
                                                                                                                         ggCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                        <  2:3908404/149‑1 (MQ=255)
                                                                                                                         ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                        <  2:4494641/149‑1 (MQ=255)
                                                                                                                           ggcTTTTCCTTTATGTTTCTAAAAATTTTTTGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                      <  2:4056026/148‑1 (MQ=255)
                                                                                                                           cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                      <  2:115810/149‑1 (MQ=255)
                                                                                                                            gcTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                     <  2:740563/149‑1 (MQ=255)
                                                                                                                              ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTT‑GTGG‑ATTTTTGGCTTTTTTCCCAGCATTCAGA‑AG‑AAAAATTCCCCGGAAATGTGAAAAAAAAAGCGACACCGCGCCAAAAACCTTCTCGa                   >  1:1126464/1‑149 (MQ=255)
                                                                                                                               tttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                  <  1:725324/149‑1 (MQ=255)
                                                                                                                                ttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACt                 <  1:2741930/149‑1 (MQ=255)
                                                                                                                                  ccAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑AG‑AAAATTTCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTAGACTCa               >  2:3951256/1‑149 (MQ=255)
                                                                                                                                    aGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa             <  1:165480/149‑1 (MQ=255)
                                                                                                                                      tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTT‑CTGG‑ATTTTTCGCTTTTTTCCCCGCATTCATA‑AG‑AAAATTTGCCGGGCATTGTGAAAAAATACCCGCCAGCGCGCAAAAACCGTTGTAGACACCTaaa           >  2:3725661/1‑149 (MQ=255)
                                                                                                                                      tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTT‑CTTG‑ATTTTTCGCTTTTTTCCCCCCATTAATA‑CG‑AAAATTGCCCCGGAAATTTGAAAAAATACGCGACAGCCCGCCAAAACCCATCTCGACACAaaaa           >  2:4445905/1‑149 (MQ=255)
                                                                                                                                       aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa          <  2:3369623/149‑1 (MQ=255)
                                                                                                                                       aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCAATCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAAAACCGTTCTCGACACATaaaa          >  2:621837/1‑149 (MQ=255)
                                                                                                                                        tGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCATTTTGCCCCCCAATAACA‑CG‑AAAAATGCCCCGGCAGTTTTAAAAAATACGCGACAGCCCGCAAAAACCCTTCTCGACTCATAAAAg         >  2:1368192/1‑149 (MQ=255)
                                                                                                                                               aaaaATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc  <  2:2600583/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGT‑CTGG‑ATTTTGCGCTTTTTGCCCAGCATTCAGA‑CG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCC  >  CP009273/450144‑450435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTG  >  CP009273/450141‑450430
                                                                                                                                                     |                                                                                                                                               
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                 >  SRR19973071.4293265/1‑150 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                >  SRR19973071.923205/1‑150 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                >  SRR19973071.1113733/1‑150 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                <  SRR19973071.14184/150‑1 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                <  SRR19973071.4653480/150‑1 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCTTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                              <  SRR19973071.3309421/150‑1 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                              <  SRR19973071.3501207/150‑1 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                              <  SRR19973071.477502/150‑1 (MQ=60)
       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                          <  SRR19973071.4718566/150‑1 (MQ=60)
          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                       >  SRR19973071.2367701/1‑150 (MQ=60)
          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                       >  SRR19973071.4369457/1‑150 (MQ=60)
           GGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAA                                                                                                                                      <  SRR19973071.4434193/150‑1 (MQ=60)
              TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                   <  SRR19973071.1363910/150‑1 (MQ=60)
              TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                   <  SRR19973071.1363941/150‑1 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                  <  SRR19973071.2916987/150‑1 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                  >  SRR19973071.1596860/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                  >  SRR19973071.4018959/1‑150 (MQ=60)
                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAACATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                              <  SRR19973071.1720790/150‑1 (MQ=60)
                    TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTG                                                                                                                             <  SRR19973071.3130939/150‑1 (MQ=60)
                     ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                            >  SRR19973071.760180/1‑150 (MQ=60)
                      CTTACACTATTAAGGATTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCG                                                                                                                           >  SRR19973071.3778667/1‑150 (MQ=60)
                      CTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCG                                                                                                                           >  SRR19973071.3778727/1‑150 (MQ=60)
                      CTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCG                                                                                                                           >  SRR19973071.3778664/1‑150 (MQ=60)
                           ACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCG                                                                                                                      >  SRR19973071.4345549/1‑150 (MQ=60)
                           ACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCG                                                                                                                      >  SRR19973071.3070392/1‑150 (MQ=60)
                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TT                                                                                                                 <  SRR19973071.2469050/150‑1 (MQ=60)
                               TTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACACATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggtt                                                                                                                  >  SRR19973071.507787/1‑146 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtt                                                                                                                 >  SRR19973071.576564/1‑146 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTG                                                                                                               >  SRR19973071.576534/1‑150 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGT                                                                                                              >  SRR19973071.1885035/1‑150 (MQ=60)
                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTT                                                                                                           <  SRR19973071.277061/150‑1 (MQ=60)
                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTT                                                                                                           <  SRR19973071.3461211/150‑1 (MQ=60)
                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTT                                                                                                           <  SRR19973071.2297940/150‑1 (MQ=60)
                                     AGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTG                                                                                                          >  SRR19973071.297327/1‑150 (MQ=60)
                                       TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTGTG                                                                                                        >  SRR19973071.2257688/1‑150 (MQ=60)
                                       TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTttg                                                                                                        >  SRR19973071.2257941/1‑147 (MQ=60)
                                       TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAATATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTG                                                                                                        >  SRR19973071.379892/1‑150 (MQ=60)
                                         GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACTGCCTGCGGGCCGT‑‑TTTtgtttttttt                                                                                                      >  SRR19973071.3644276/1‑140 (MQ=60)
                                         GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgttttttgtg                                                                                                        >  SRR19973071.3002537/1‑136 (MQ=60)
                                         GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtg                                                                                                      >  SRR19973071.3644937/1‑145 (MQ=60)
                                          GAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCTTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGG                                                                                                     <  SRR19973071.4053695/150‑1 (MQ=60)
                                          GAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttggtttttgtgg                                                                                                       >  SRR19973071.1793171/1‑136 (MQ=60)
                                          GAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtggttttgttttt                                                                                                       >  SRR19973071.1305593/1‑136 (MQ=60)
                                          GAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTGTgttg                                                                                                     >  SRR19973071.4191390/1‑146 (MQ=60)
                                              GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTT                                                                                                 <  SRR19973071.4245346/150‑1 (MQ=60)
                                               GTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtggttttt                                                                                                  >  SRR19973071.3777579/1‑131 (MQ=60)
                                               GTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttgtgttgttttt                                                                                                  >  SRR19973071.3777578/1‑131 (MQ=60)
                                               GTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgttgttttt                                                                                                >  SRR19973071.2430064/1‑139 (MQ=60)
                                               GTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTGTGTGGGTTTT                                                                                                >  SRR19973071.3482695/1‑150 (MQ=60)
                                                TTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTG                                                                                               <  SRR19973071.183040/150‑1 (MQ=60)
                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtggtttttgtggtttttttgg                                                                                              >  SRR19973071.1228840/1‑127 (MQ=60)
                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTGTGTGGATTTTtggc                                                                                            >  SRR19973071.4708378/1‑146 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTT                                                                                          <  SRR19973071.2202782/150‑1 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTT                                                                                          <  SRR19973071.1509362/150‑1 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtgggtttttggggt                                                                                          >  SRR19973071.2249599/1‑133 (MQ=60)
                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAAAGGCCTGCGGGCCGTgttttttttttttgtttttttggtttt                                                                                          >  SRR19973071.3828135/1‑123 (MQ=60)
                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTT                                                                                        <  SRR19973071.332978/150‑1 (MQ=60)
                                                       ACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTAGCGCTTTT                                                                                        <  SRR19973071.4141765/150‑1 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtggggttttggggtttt                                                                                       >  SRR19973071.4478793/1‑130 (MQ=60)
                                                           CGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGCCCAGCGGGCCGT‑‑GTTGTTTTGTCGGGGTTTTGCGCTTTTTGCC                                                                                    >  SRR19973071.2387031/1‑150 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttggtgttttttgggttttttgcc                                                                                   >  SRR19973071.1684215/1‑126 (MQ=60)
                                                             TCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                  <  SRR19973071.2097680/150‑1 (MQ=60)
                                                             TCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                  <  SRR19973071.1713635/150‑1 (MQ=60)
                                                             TCTTTGCCTCTCCTCCCTGTCGTGGAGAATTAAGCAGCGCGTAAAAACGCATTTGAAACTGTCGGCTCTTTTCCAGTATGTTTCTAAATATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                  <  SRR19973071.4111855/150‑1 (MQ=60)
                                                              CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑‑GGTGTTTTGTCGGGGTTTTGCGCTTTTTGCCCcc                                                                                 >  SRR19973071.1063039/1‑148 (MQ=60)
                                                              CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTGGCTGGAGTTTGCGCTTTTTTCCCCG                                                                                 >  SRR19973071.1062976/1‑150 (MQ=60)
                                                                 TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑‑TTTGTTTTGTGTTGCTTTTTAGCTTTTTTCCCAGCAT                                                                              >  SRR19973071.401561/1‑150 (MQ=60)
                                                                  GCCTCTCCGCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTGGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT                                                                             <  SRR19973071.4015566/150‑1 (MQ=60)
                                                                     TCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTGGTTTTGTCTGGATTTTGCGCTTTTTTCCCCGCAATCAG                                                                          >  SRR19973071.1122552/1‑150 (MQ=60)
                                                                      CTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTGGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA                                                                         <  SRR19973071.4450573/150‑1 (MQ=60)
                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAC                                                                        <  SRR19973071.3744038/150‑1 (MQ=60)
                                                                        CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttgtttggtgtttttgcttttttgccagcaataatacg                                                                         >  SRR19973071.4648901/1‑106 (MQ=60)
                                                                         CTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GGTGTTTTTTCTGGAGTTTTCGCTTTTTGGCCAGCAataagacca                                                                      >  SRR19973071.3024517/1‑141 (MQ=60)
                                                                          TCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTtttttggttttggggttttttgccaccagtcaaaaaca                                                                     >  SRR19973071.4600642/1‑112 (MQ=60)
                                                                           CCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttttttttttgggttttggggtttttggccaccagtaagaagaaa                                                                      >  SRR19973071.1730235/1‑103 (MQ=60)
                                                                           CCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTGTCTGGATTTTGCGCGTTTTGCCCAGCAGTCAGACG‑AAA                                                                   >  SRR19973071.289619/1‑150 (MQ=60)
                                                                            CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAA                                                                  <  SRR19973071.2389862/150‑1 (MQ=60)
                                                                            CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAA                                                                  <  SRR19973071.2389977/150‑1 (MQ=60)
                                                                              TGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TGTGTTTTttgtgggtttttggcgtttttgccaccaataacaccaaaaaa                                                                 >  SRR19973071.2869749/1‑108 (MQ=60)
                                                                                 CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTGTGGATTTTTCGGTTTTTGCCCACCATTAAGACG‑AAAATTGCC                                                             >  SRR19973071.3915684/1‑150 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                            <  SRR19973071.1071128/150‑1 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGG                                                          <  SRR19973071.467784/150‑1 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGG                                                          <  SRR19973071.1851937/150‑1 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtggttttttgggttttttgccaccaatcatgcgaaaattgcccgg                                                           >  SRR19973071.3365640/1‑102 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAT                                                      <  SRR19973071.4631829/150‑1 (MQ=60)
                                                                                          GGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTG                                                    <  SRR19973071.685013/150‑1 (MQ=60)
                                                                                           GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGT                                                   <  SRR19973071.1074183/150‑1 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtggtttttggggtttttggccaccaatcagacgaaaaattacccgggcttgtg                                                     >  SRR19973071.3324801/1‑86 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTG‑TTTGTTTTtgtgggttttgggggtttttgcccgcaataagaccaaaaattcccgggaattgtg                                                    >  SRR19973071.3423983/1‑95 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTttttgggtttttgggttttttgccagcaataagacgaaaaattccccggcaatgtg                                                   >  SRR19973071.3324773/1‑94 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtggttttttggcttttttgacagcaataagaacaaaatttcgccgggaatttg                                                   >  SRR19973071.3324766/1‑94 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtgggtttttggcttttttgcccccaatcagaaccaaaatgccccggacgtgtg                                                   >  SRR19973071.1370183/1‑94 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgggggttttgggggttttggcccgccgtaacacgaaaaattcccggggcgtggg                                                   >  SRR19973071.1370161/1‑94 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑‑TTTGTTTTttggtgtgttttggcttttttgccagccataagacgaaaaatggcccgggaatgtg                                                   >  SRR19973071.3324762/1‑94 (MQ=60)
                                                                                               CATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttggtggtttttggggtttttggccgccatcagaagaaaaattgcccgggattgtgtaa                                                >  SRR19973071.2176469/1‑91 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtgggttttgggcttttttacccccaaacagaccaaaaattgcccgggaatgtgtaaaaataa                                          >  SRR19973071.684838/1‑85 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTACTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtgggtttttgggttttttgcccccaatacaaagaaaaattgcccggcatattgtaaaaaaaa                                          >  SRR19973071.545466/1‑85 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTACTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTGTGTGGTGTTTGCGCTTTTTTCCCCGCAATAAGACG‑AAAAATGCCCGGGGAGTGTGAAAAAATAA                                         >  SRR19973071.2383768/1‑150 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgtgtgttttttgtggttttttggggtttttggcagcaatcagacgcaaaattcccggggattttgtaaaaataag                                           >  SRR19973071.3269167/1‑75 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtttttttgtggttttttggtttttttgcccccataagaaggaaaaatgcccgggagttgttaaaaataaa                                           >  SRR19973071.3606193/1‑76 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTtttttggtttttgggttttttggccggaataaaaacgaaaattgcccgggagtttttaaaaaaaaa                                         >  SRR19973071.3269373/1‑84 (MQ=60)
                                                                                                       AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTTTGTGGGTTTTGGGGTTTTTGGCCAGCATTAAGACG‑AAAATTTGCCGGGAATTGTGTAAAAATAAGC                                       >  SRR19973071.786506/1‑150 (MQ=60)
                                                                                                        ACAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTGGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                      <  SRR19973071.3446512/150‑1 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTTTCGGGATTTTGCGGTTTTTtcccccccatcagaccaaaaatgcccgggcaatgtggaaaaaaaaccc                                       >  SRR19973071.4323430/1‑102 (MQ=60)
                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGAC                                    <  SRR19973071.1730150/150‑1 (MQ=60)
                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGAC                                    <  SRR19973071.1730283/150‑1 (MQ=60)
                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGAC                                    <  SRR19973071.1730172/150‑1 (MQ=60)
                                                                                                            CGCATTTGCAACTGTGGGCGCTTTTCCTGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAG                                  <  SRR19973071.40611/150‑1 (MQ=60)
                                                                                                            CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTTCTAAAGATTTTATTAAAAAAGGCCTGCGGGCCGTGGTTTGTTTTGTgtttttttggcttttttgcacccaataagagcaaaaaataccgcggcatttgaaaaaaaaaaaacgac                                     >  SRR19973071.1411405/1‑82 (MQ=60)
                                                                                                            CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTTTGGGGTTTTTGGGCTTTTTGCCCAGCAataataacaaaaattgccggggaatttttaaaaataagcgaccg                                   >  SRR19973071.3157973/1‑106 (MQ=60)
                                                                                                            CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggttgtttttgtgggtttttggggtttttggccacaataacacgaaaaaatgcccgggcaggttgaaaaaaaaaccacag                                     >  SRR19973071.1649631/1‑69 (MQ=60)
                                                                                                            CGAATTTGAAAGTGTCGGCGCTTTTCCTTTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAG                                  <  SRR19973071.40334/150‑1 (MQ=60)
                                                                                                                TTTGCAAGTGTCGGGGCTTTTCCTGTTTGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCG                              <  SRR19973071.4231121/150‑1 (MQ=60)
                                                                                                                    CAACTGTGGGCGCTTTTCCTTTATGTTGCTAAAATTTTTTTGAAAAAGGGCCTGGGGGCCGT‑‑TTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAAT                          <  SRR19973071.461189/150‑1 (MQ=60)
                                                                                                                    CAACTGTCGGCGCTTTTCCTTTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAT                          <  SRR19973071.2862369/150‑1 (MQ=60)
                                                                                                                    CAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTtttttttgttgttttttggcttttttgccagccataagacgaaaatttccccggaattgtggaaaaaaaaccgacagcgccgcaa                           >  SRR19973071.2665320/1‑65 (MQ=60)
                                                                                                                        TGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACC                      <  SRR19973071.4302697/150‑1 (MQ=60)
                                                                                                                         GTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCG                     <  SRR19973071.3326125/150‑1 (MQ=60)
                                                                                                                         GTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCG                     <  SRR19973071.121308/150‑1 (MQ=60)
                                                                                                                         GTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCG                     <  SRR19973071.720780/150‑1 (MQ=60)
                                                                                                                         GTCGGCGCTTTGCCTGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCG                     <  SRR19973071.4664416/150‑1 (MQ=60)
                                                                                                                          TCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttttttttgtggagttttagcgttttttaacccaataaagagaaaaattgcccgggaatttgttaaaaaaacaaacacagcgccaaaaacca                       >  SRR19973071.2098198/1‑56 (MQ=60)
                                                                                                                             GCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                 <  SRR19973071.4685103/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttttgggtttttttgttttttgccccccaaaagaacaaaaattgcccgggagtttttaaaaaaaaaacacaacccccccaaaaacgaaaa                 >  SRR19973071.1662471/1‑60 (MQ=60)
                                                                                                                              CGATTTTCCAGTTTGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                <  SRR19973071.4546149/150‑1 (MQ=60)
                                                                                                                              CGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                <  SRR19973071.3764220/150‑1 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtggttttttggcttttttcccagcaaaaagacgaaaaattccccgggattgttaaaaaataatcgacagcgcgcaaaaacccatctcg                  >  SRR19973071.657234/1‑51 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttatggttttttggcttttttcacaccaataagaccaaaaattcccccgcaaagttaaaaaaaaaacgaccccccgcaaaaaaacgtaacc                  >  SRR19973071.3354545/1‑51 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTTTTTTGTCTGGATTTTTCGCTTTTTtgccaccaataagaccaaaatttccccgggaatttgtaaaaataaacgacaccgcgccaaaaaccgaatac                >  SRR19973071.657095/1‑79 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttgtgggtgtttggcttttttgccacccaaacgacccaaaaatccccggcacattgaaaaaaaaaaccacccccccccaaaaaccgtctcc                >  SRR19973071.3710142/1‑59 (MQ=60)
                                                                                                                                CTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTttttttttttggtttttggcttttttaccagcaataaaaacaaaaaatacccggaaattttaaaaaaaaacccacaccccccaaaaaaccttatcga               >  SRR19973071.2930369/1‑53 (MQ=60)
                                                                                                                                CTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTTTGTGGATTTTTGGCTTTTTTCCCAGCATTCAGAAG‑AAAAATTCCCCGGAAATGTGAAAAAAaaagcgacaccgcgccaaaaaccttctcga              >  SRR19973071.1130414/1‑120 (MQ=60)
                                                                                                                                CTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTTTATGGATTTTGCGCATTTTTCCCACCATTAAAACGAAAAATTGCCCGGCAA‑TGTGAAAAAATAAGCGACAGCCCGCAAAAACCGTTCTAGA              >  SRR19973071.1130352/1‑150 (MQ=60)
                                                                                                                                CTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTTGGTTTTTCGCTTTTTGCCCAGCATTCATACG‑AAAATTGCCCCGGACATGTTAAAAAAAACGCGACAGCGCGCAAaaaaccttatcga              >  SRR19973071.1200917/1‑137 (MQ=60)
                                                                                                                                 TTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTTTCTGTATTTTTGGCTTTTTGCCCAccaataagaacaaaaattccagggaattttttaaaaataagagacaccgcccaaaaaaacttctagac              >  SRR19973071.1507423/1‑82 (MQ=60)
                                                                                                                                  TTTCCCGTCTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACT            <  SRR19973071.2196318/150‑1 (MQ=60)
                                                                                                                                  TTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACT            <  SRR19973071.727841/150‑1 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTTTTTTTTATGGATTTTTCGCTTTTTTCCCAGCATTAATACG‑AAAATTTCCCCGGAATTTTTAAAAAATACGCGACAGCCCGCAATAAACGTTCTCGAATC           >  SRR19973071.2394449/1‑150 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTC           <  SRR19973071.2751649/150‑1 (MQ=60)
                                                                                                                                   TTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTTTATGGATTTTGCGCTTTTTGGCCACCCATCATACG‑AAAATTGCCCGGGGATTGTGAAAAAATACGCGACAGCGCGCAAAAACCGGTCTCGACTC           >  SRR19973071.2394480/1‑150 (MQ=60)
                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgttgttttttggcttttttccagcaataaaaagaaaatttccccgggattttgtaaaaaaaaacgacacaccgcaaaaaaacttatagacacaaaa           >  SRR19973071.2186222/1‑44 (MQ=60)
                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTTTGTGGATTTTGCGCTTTTTTCCCAGCAATAAGAAG‑AAAAATTCCCGGGAAATTTGTAAAAAAAAGCGACAGCGCGCAATAAACGTTGTCGACTCATA        >  SRR19973071.2785633/1‑150 (MQ=60)
                                                                                                                                      CAGTATGTTGCTAAAAATTTAATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTtttcgtttttttaggattttttccagcaatcatcccaaaaattccacgggatttttgaaaaaaaaacgaaaacgcgccaaaaaacttcacgaaaacaa         >  SRR19973071.2785451/1‑52 (MQ=49)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAA       <  SRR19973071.166200/150‑1 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAA       <  SRR19973071.166426/150‑1 (MQ=60)
                                                                                                                                       AGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAA       <  SRR19973071.166407/150‑1 (MQ=60)
                                                                                                                                          ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑GTTGTTTTttttttttttttggcttttttgccaccaaaaaaaaaaaaaaatgcccggccatgtttaaaaaaaaaacaaaccccgcacaaaacaatcaccacaaaaaaaaa     >  SRR19973071.4570095/1‑48 (MQ=60)
                                                                                                                                           TGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGT   <  SRR19973071.2597012/150‑1 (MQ=60)
                                                                                                                                            GTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTG  <  SRR19973071.1288926/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                               
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTG  >  CP009273/450141‑450430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑