Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA92.1% 653.0 / 33.7 202intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (7/9);  new base A (80/106);  total (87/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 203 total aligned reads displayed.

TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450143‑450436
                                                                                                                                                   |                                                                                                                                                   
tCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt                                                                                                                                                   <  2:941252/150‑1 (MQ=255)
tCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt                                                                                                                                                   >  2:3604342/1‑150 (MQ=255)
 cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                  <  1:4615736/150‑1 (MQ=255)
 cATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                  <  1:1653530/150‑1 (MQ=255)
  aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGTAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt                                                                                                                                                 <  1:3499888/150‑1 (MQ=255)
  aTGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt                                                                                                                                                 >  1:3725863/1‑150 (MQ=255)
     cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                              <  2:4106413/150‑1 (MQ=255)
      gcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATg                                                                                                                                             >  1:3299533/1‑150 (MQ=255)
           ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATTTTGCTAAAAATTTTATGaaaaa                                                                                                                                        >  2:2970491/1‑150 (MQ=255)
           ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                        >  1:2023939/1‑150 (MQ=255)
           ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                        >  1:2024142/1‑150 (MQ=255)
            tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                       <  2:4769517/150‑1 (MQ=255)
            tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTGTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                       <  2:4478301/150‑1 (MQ=255)
              tGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACgg                                                                                                                                     <  2:4314253/150‑1 (MQ=255)
               gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                    >  1:4412604/1‑150 (MQ=255)
                cATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                   >  1:2515875/1‑150 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCt                                                                                                                                  <  2:456725/150‑1 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCt                                                                                                                                  <  2:1230726/150‑1 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                  >  1:1534996/1‑150 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                  >  1:67758/1‑150 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                  >  1:4071828/1‑150 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                  >  1:3945525/1‑150 (MQ=255)
                   aCTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGc                                                                                                                                >  1:3680096/1‑150 (MQ=255)
                          cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt                                                                                                                         >  1:1962179/1‑150 (MQ=255)
                           tATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                        >  2:2611897/1‑150 (MQ=255)
                            aTTATGGAGTGGGACGGGTTGCAGGACACCGTATTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCTTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTTTGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCgttt                                                                                                                       <  1:846968/150‑1 (MQ=255)
                            aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCggtt                                                                                                                       >  2:3819734/1‑150 (MQ=255)
                            aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCggtt                                                                                                                       >  1:2647455/1‑150 (MQ=255)
                            aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCgttt                                                                                                                       <  1:1834217/150‑1 (MQ=255)
                              tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                     >  2:1518563/1‑150 (MQ=255)
                                   aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                                                <  2:2167975/150‑1 (MQ=255)
                                   aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                                                <  2:1224173/150‑1 (MQ=255)
                                    gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTtttgttttgt                                                                                                               <  2:2100379/150‑1 (MQ=255)
                                     tGGGAAGGGTGGCAGGACACCGTCTGGGCCGCTCCGCCCTGTCGCGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACGGTCGGCGCTTTTCCAGCAGGTTGCTAAAAATTTTATGACAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                              <  2:4723970/150‑1 (MQ=255)
                                      gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTtgtgttttgtgt                                                                                                             >  1:723497/1‑148 (MQ=255)
                                      gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCt                                                                                                             >  2:947162/1‑150 (MQ=255)
                                         aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGa                                                                                                          >  2:2620470/1‑150 (MQ=255)
                                            ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTTGAttt                                                                                                       >  2:637839/1‑150 (MQ=255)
                                             gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                      <  1:2899836/150‑1 (MQ=255)
                                               tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTGGTGGAGCAGGAAGCATCGAGTAAAAACGCATTTGCAACGGTCGGCGATTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                                                    <  1:4478009/150‑1 (MQ=255)
                                               tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                                                    >  2:2786348/1‑150 (MQ=255)
                                               tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTgc                                                                                                    >  2:394702/1‑150 (MQ=255)
                                                      cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                             <  2:3010696/150‑1 (MQ=255)
                                                      cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTGGATTTTGCGCttttt                                                                                             >  2:4236289/1‑150 (MQ=255)
                                                       acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                            <  2:3478039/150‑1 (MQ=255)
                                                        ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGc                                                                                           >  2:1944203/1‑150 (MQ=255)
                                                        ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTATGGATTTTTCGCTTTTTGc                                                                                           >  2:2455632/1‑150 (MQ=255)
                                                               tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                    <  1:1982955/150‑1 (MQ=255)
                                                               tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                    <  2:4443472/150‑1 (MQ=255)
                                                               tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                    <  2:3592977/150‑1 (MQ=255)
                                                               tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAt                                                                                    <  2:2040276/150‑1 (MQ=255)
                                                                   tcgcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATGTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                <  1:4458186/147‑1 (MQ=255)
                                                                      cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                             <  2:1427624/150‑1 (MQ=255)
                                                                      cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                             <  1:4733996/150‑1 (MQ=255)
                                                                      cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                             <  1:4731786/150‑1 (MQ=255)
                                                                       ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTATGGATTTTGCGCTTTTTTCCCAGCAATCAGAcca                                                                            >  2:3275319/1‑148 (MQ=255)
                                                                          ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑anaa                                                                        <  2:25567/150‑1 (MQ=255)
                                                                          ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGTGGATTTTTCGGTTTTTGCCCAGCAAACACACG‑aaaa                                                                        >  1:3902510/1‑150 (MQ=255)
                                                                          ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCTTGTTTTTGGGGGTTTTGGACACCCATCACACG‑aaaa                                                                        >  1:3828294/1‑150 (MQ=255)
                                                                             gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTg                                                                     <  1:2874971/150‑1 (MQ=255)
                                                                                 tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg                                                                 <  2:64850/150‑1 (MQ=255)
                                                                                 tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg                                                                 <  1:2897933/150‑1 (MQ=255)
                                                                                 tGGAACAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCTGTTTGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg                                                                 <  1:589647/150‑1 (MQ=255)
                                                                                   gAGCAGGAAGCATCGCATAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTGCGCTTTTTGCCCAGCCTTAAGACG‑AAAAATGCCCggg                                                               >  1:3049896/1‑150 (MQ=255)
                                                                                       tggAAGCATCGCTTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCATTTTGTTGCTAAAATTTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt                                                           <  1:4014447/149‑1 (MQ=255)
                                                                                       agtaaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt                                                           <  1:4278957/147‑1 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTTCGGATTTTTACCAGCAATAAGACG‑AAAAATTCCCggggaat                                                           >  1:4345199/1‑146 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt                                                           <  1:2161800/150‑1 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAtt                                                           <  1:2161669/150‑1 (MQ=255)
                                                                                          aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGGGGTTTTTTCCCAGCATTCAGACG‑AAAAATTCCCGGGAATtgtg                                                        >  1:3971626/1‑150 (MQ=255)
                                                                                          aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtgtg                                                        <  1:2786348/150‑1 (MQ=255)
                                                                                          aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTGTGTGGTTTTTGGGCTTTTTTCACAGCAATGAGACG‑CAAATTGCCCGGGACTtgtt                                                        >  2:2138961/1‑149 (MQ=255)
                                                                                              aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGaaaa                                                    <  2:3521248/150‑1 (MQ=255)
                                                                                                 gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTAAGACG‑AAAATTTCCCGGGAAATGTGTAAAAAt                                                 >  2:2267352/1‑150 (MQ=255)
                                                                                                  cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTTGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATa                                                <  2:464160/150‑1 (MQ=255)
                                                                                                   gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAc                                               <  2:3725863/150‑1 (MQ=255)
                                                                                                     aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                             <  1:4659885/150‑1 (MQ=255)
                                                                                                      aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                            <  1:2402124/150‑1 (MQ=255)
                                                                                                      aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTTGGCTTTTTGGCCACCAATAAGAAG‑AAAAATGCCCGGGCAATGTGGAAAAAAAagcg                                            >  1:4311329/1‑150 (MQ=255)
                                                                                                         aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                                         <  2:3469260/150‑1 (MQ=255)
                                                                                                         aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                                         <  1:3181027/150‑1 (MQ=255)
                                                                                                           gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCGGGATTTTTGGCGTTTTTCCCAGCCCTCAGACG‑AAAATTGGCCGGGAATGTGGTAAAAATAACCGACaac                                       >  1:1887135/1‑148 (MQ=255)
                                                                                                            cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTTGATTTTTGGCATTTTGGCCAGCCATAAGGCGAAAAATTGCCC‑GGAATTGTGAAAAAATACGCGACcgcg                                      >  2:63575/1‑150 (MQ=255)
                                                                                                                 gCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                                 <  2:4468038/150‑1 (MQ=255)
                                                                                                                  cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGGTTTGTTTTTTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGTCG‑AAAAATGCCCGGGACTTGTTAAAAAATACGCGCCCGCGCGCAAt                                >  2:3889014/1‑150 (MQ=255)
                                                                                                                   aagtGTCGGCGCTTTTCCAGTATTTTGCTAAAGATTTTATGAAAAACGGCGTGCGGGCCTTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCTTTCAGACG‑AAAATTGCCCGTGAATTGTGAAAAAATACGCGACAGCGCGCAATa                               <  1:2713366/147‑1 (MQ=255)
                                                                                                                   aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                               <  2:470767/150‑1 (MQ=255)
                                                                                                                   aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTTTGTTGGTTTTTGGCTTTTTGTCCCGCAATAACAAGAAAAAATCCCCGGGAATT‑TGTAAAAAAAAGCGACACCGCCCaaaa                               >  1:4175596/1‑148 (MQ=255)
                                                                                                                   aaCTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                               <  1:3148869/150‑1 (MQ=255)
                                                                                                                     cTGTCGGCGCTTTTCCATTTTGTTGCTAAAATTTTTAGCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                             <  1:524236/150‑1 (MQ=255)
                                                                                                                     cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                             <  2:1882334/150‑1 (MQ=255)
                                                                                                                           gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                       <  2:1710488/150‑1 (MQ=255)
                                                                                                                                tttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCAATCAGACG‑AAAAATTCCCGGGAATTTTGAAAAAATAAGCGACAGCGCGCAATAAACGTTCTCGACt                  >  2:4364701/1‑150 (MQ=255)
                                                                                                                                  tCCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa                <  2:2484384/150‑1 (MQ=255)
                                                                                                                                    cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATa              <  2:1383743/150‑1 (MQ=255)
                                                                                                                                     aGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaa             <  2:4514366/150‑1 (MQ=255)
                                                                                                                                      gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa            <  1:105151/150‑1 (MQ=255)
                                                                                                                                          gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTTAGCTTTTTGCCCCGCATTCAGACG‑AAAATTGCCCGGGGATTGTGAAAAAATAAGAGACAGCGCGCAATAAACGGTCTAGACTCCAAAAAgag        >  2:3392552/1‑148 (MQ=255)
                                                                                                                                              cTAAAAATTTCATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc    <  2:3298205/150‑1 (MQ=255)
                                                                                                                                               tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTGGTTGATTTTTAGCTTTTTTCCCAGCAGTCAGACG‑AAAAATGCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc   >  2:4194858/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450143‑450436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGC  >  CP009273/450144‑450437
                                                                                                                                                  |                                                                                                                                                   
CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                  <  SRR19973078.1663553/150‑1 (MQ=60)
CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                  <  SRR19973078.4641883/150‑1 (MQ=60)
 ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGTAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTT                                                                                                                                                 <  SRR19973078.3519773/150‑1 (MQ=60)
 ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTT                                                                                                                                                 >  SRR19973078.3745559/1‑150 (MQ=60)
 ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTT                                                                                                                                                 >  SRR19973078.3747234/1‑150 (MQ=60)
     GCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGTATGTTGCTAAAAATTTTATG                                                                                                                                             >  SRR19973078.3318349/1‑150 (MQ=60)
          CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                        >  SRR19973078.4691624/1‑150 (MQ=60)
          CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                        >  SRR19973078.2035972/1‑150 (MQ=60)
          CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                        >  SRR19973078.2035767/1‑150 (MQ=60)
          CTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATATGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAA                                                                                                                                        >  SRR19973078.2035916/1‑150 (MQ=60)
           TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                       <  SRR19973078.2711216/150‑1 (MQ=60)
           TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAA                                                                                                                                       >  SRR19973078.4642276/1‑150 (MQ=60)
             TGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAACGG                                                                                                                                     >  SRR19973078.4285045/1‑150 (MQ=60)
             TGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGG                                                                                                                                     >  SRR19973078.2367396/1‑150 (MQ=60)
              GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                    >  SRR19973078.4437396/1‑150 (MQ=60)
              GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                    >  SRR19973078.258529/1‑150 (MQ=60)
               CATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCC                                                                                                                                   >  SRR19973078.2530310/1‑150 (MQ=60)
                ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  >  SRR19973078.68477/1‑150 (MQ=60)
                ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  >  SRR19973078.1544204/1‑150 (MQ=60)
                ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  >  SRR19973078.4095031/1‑150 (MQ=60)
                ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                  >  SRR19973078.3967877/1‑150 (MQ=60)
                  ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGC                                                                                                                                >  SRR19973078.3701122/1‑150 (MQ=60)
                         CTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT                                                                                                                         >  SRR19973078.1973599/1‑150 (MQ=60)
                           ATTATGGAGTGGGACGGGTTGCAGGACACCGTATTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCTTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTTTGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTT                                                                                                                       <  SRR19973078.852075/150‑1 (MQ=60)
                           ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTT                                                                                                                       >  SRR19973078.2662549/1‑150 (MQ=60)
                           ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTT                                                                                                                       <  SRR19973078.1845241/150‑1 (MQ=60)
                             TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG                                                                                                                     <  SRR19973078.2729043/150‑1 (MQ=60)
                                 GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgtttt                                                                                                                 >  SRR19973078.2090559/1‑141 (MQ=60)
                                  AGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTT                                                                                                                >  SRR19973078.3836382/1‑150 (MQ=60)
                                   GTGGGACGGGTTGCAGGACACCGTATTTGCCTCTCCCCCCTGTCGTGGAGCAGGAAGAATCGCTTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGT                                                                                                               <  SRR19973078.818950/150‑1 (MQ=60)
                                     GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtgtgtttttgt                                                                                                             >  SRR19973078.2498646/1‑138 (MQ=60)
                                     GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGTGT                                                                                                             >  SRR19973078.727903/1‑150 (MQ=60)
                                       GAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggggggtttttgtgg                                                                                                           >  SRR19973078.3718162/1‑135 (MQ=60)
                                       GAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGG                                                                                                           <  SRR19973078.184872/150‑1 (MQ=60)
                                         AGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtttttttttttggt                                                                                                         >  SRR19973078.4157352/1‑133 (MQ=60)
                                          GGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATT                                                                                                        <  SRR19973078.4189277/150‑1 (MQ=60)
                                            GTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT                                                                                                      <  SRR19973078.2916347/150‑1 (MQ=60)
                                              TGCAGGACACCGTCTTTGCCTCTCCTCCCTGTGGTGGAGCAGGAAGCATCGAGTAAAAACGCATTTGCAACGGTCGGCGATTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGC                                                                                                    <  SRR19973078.4503354/150‑1 (MQ=60)
                                              TGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGC                                                                                                    <  SRR19973078.161630/150‑1 (MQ=60)
                                               GCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTTGGTTTTTGTGGAGTTTGCG                                                                                                   >  SRR19973078.3941629/1‑150 (MQ=60)
                                                      ACCGTATTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                            <  SRR19973078.1527735/150‑1 (MQ=60)
                                                        CGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACCGTATGTTGCTAAAAAATTTATGAAAAACGGCCTGCGGGCCcgggtggtttttgtgtgggtttgggtgttttga                                                                                          >  SRR19973078.2180919/1‑117 (MQ=60)
                                                         GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgttgtgtttttggttttttgac                                                                                         >  SRR19973078.2443648/1‑117 (MQ=60)
                                                         GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCC                                                                                         <  SRR19973078.612359/150‑1 (MQ=60)
                                                         GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTGTgtgggtgtgtggggttttggcc                                                                                         >  SRR19973078.966126/1‑128 (MQ=60)
                                                           CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAG                                                                                       <  SRR19973078.2976485/150‑1 (MQ=60)
                                                              TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                    <  SRR19973078.3081636/150‑1 (MQ=60)
                                                              TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                    <  SRR19973078.1994531/150‑1 (MQ=60)
                                                              TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                    <  SRR19973078.1994433/150‑1 (MQ=60)
                                                                CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTC                                                                                  <  SRR19973078.4380757/150‑1 (MQ=60)
                                                                CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTC                                                                                  <  SRR19973078.3412982/150‑1 (MQ=60)
                                                                  TCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAG                                                                                <  SRR19973078.4485527/150‑1 (MQ=60)
                                                                  TCGCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATGTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAG                                                                                <  SRR19973078.4483376/150‑1 (MQ=60)
                                                                   CTCCTCCCTGTCGTGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggttttttttgttttttgggtttttttggagcccagcga                                                                               >  SRR19973078.3618362/1‑107 (MQ=60)
                                                                     CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgtggggttttttttggttttttgggttttttgcaggcaaagataa                                                                             >  SRR19973078.2409776/1‑105 (MQ=60)
                                                                     CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG                                                                             <  SRR19973078.4758400/150‑1 (MQ=60)
                                                                     CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG                                                                             <  SRR19973078.4758538/150‑1 (MQ=60)
                                                                     CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG                                                                             <  SRR19973078.4760618/150‑1 (MQ=60)
                                                                         CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGTGGATTTTTCGGTTTTTGCCCAGCAAACACACGAAAA                                                                         >  SRR19973078.3924740/1‑150 (MQ=60)
                                                                         CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttcttgtttttgggggttttggacacccatcacacgaaaa                                                                         >  SRR19973078.3850226/1‑110 (MQ=60)
                                                                         CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTtggcttttttcacacccatcagaccaaaa                                                                         >  SRR19973078.3924711/1‑121 (MQ=60)
                                                                            GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                      <  SRR19973078.2891277/150‑1 (MQ=60)
                                                                            GGCGTGGAGCAGGACGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                      <  SRR19973078.2890441/150‑1 (MQ=60)
                                                                              CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTATGGATTTTTGGCTTTTTTCCCAGCAATAAGACGAAAAATGCC                                                                    >  SRR19973078.4007668/1‑150 (MQ=60)
                                                                                TGTAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCG                                                                  <  SRR19973078.2914688/150‑1 (MQ=60)
                                                                                TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCG                                                                  <  SRR19973078.2914429/150‑1 (MQ=60)
                                                                                TGGAACAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCG                                                                  <  SRR19973078.592220/150‑1 (MQ=60)
                                                                                TGGAACAGGAAGCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCTGTTTGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCG                                                                  <  SRR19973078.593531/150‑1 (MQ=60)
                                                                                  GAGCAGGAAGCATCGCATAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGTTTTTGCGCTTTTTGCCCAGCCTTAAGACGAAAAATGCCCGGG                                                                >  SRR19973078.3067182/1‑150 (MQ=60)
                                                                                     CTGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTGTCCAGTATGTTGCTAAAATTTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                             <  SRR19973078.4464038/150‑1 (MQ=60)
                                                                                     CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                             <  SRR19973078.1987062/150‑1 (MQ=60)
                                                                                      TGGAAGCATCGCTTAAAAACGCATTTGCAACTGTGGGCGCTTTTCCATTTTGTTGCTAAAATTTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.4037155/150‑1 (MQ=60)
                                                                                      AGTAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.4303227/150‑1 (MQ=60)
                                                                                      AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTtcggatttttaccagcaataagacgaaaaattcccggggaat                                                            >  SRR19973078.4369665/1‑108 (MQ=60)
                                                                                      AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.3171189/150‑1 (MQ=60)
                                                                                      AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.1886794/150‑1 (MQ=60)
                                                                                      AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.2174461/150‑1 (MQ=60)
                                                                                      AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.2174329/150‑1 (MQ=60)
                                                                                      AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATT                                                            <  SRR19973078.2174443/150‑1 (MQ=60)
                                                                                         AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGATTTTGGGGTTTTTTCCCAGCATTCAGACGAAAAATTCCCGGGAATTGTG                                                         >  SRR19973078.3994058/1‑150 (MQ=60)
                                                                                         AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG                                                         <  SRR19973078.397044/150‑1 (MQ=60)
                                                                                         AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTG                                                         <  SRR19973078.2801865/150‑1 (MQ=60)
                                                                                          AGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGA                                                        <  SRR19973078.493715/150‑1 (MQ=60)
                                                                                           GCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAA                                                       <  SRR19973078.2902521/150‑1 (MQ=60)
                                                                                                    AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttttgggttttttggttttttgccccccaacaggcaacaaatttccgggggatgtgttaaaataaag                                              >  SRR19973078.3280348/1‑75 (MQ=60)
                                                                                                    AAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGC                                              <  SRR19973078.4686246/150‑1 (MQ=60)
                                                                                                     AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTgtgtttttttgtgggtttttggggtttttggccgccttaaggcgaaaaattcccggggcatgtgtaaaaataagcg                                             >  SRR19973078.4316533/1‑74 (MQ=60)
                                                                                                     AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCG                                             <  SRR19973078.2415681/150‑1 (MQ=60)
                                                                                                     AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgttgttttttgggttttttgccagcaataagaagaaaaatggccggggaatgtgtaaaaaaaaccc                                             >  SRR19973078.4336522/1‑82 (MQ=60)
                                                                                                     AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTtggctttttggccaccaataagaagaaaaatgcccgggcaatgtggaaaaaaaagcg                                             >  SRR19973078.4335691/1‑93 (MQ=60)
                                                                                                     AAAACGCATTTGCAACTGTCGGCGCTTTTCAAGTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGGGTTGTTTTGTttttgttttttgggttttttgcaagcaggaggcgaaaaaaggccccgcaatattaataaaaaaaag                                             >  SRR19973078.1350133/1‑84 (MQ=60)
                                                                                                        ACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACA                                          <  SRR19973078.3198715/150‑1 (MQ=60)
                                                                                                        ACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACA                                          <  SRR19973078.3198965/150‑1 (MQ=60)
                                                                                                        ACGAATTTGAAACTGTCGGCGCTTTTCCTTTATGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACA                                          <  SRR19973078.4077941/150‑1 (MQ=60)
                                                                                                          GCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTCGGGATTTTTGGCGTTTTTCCCAGCCCTCAGACGAAAATTGGCCGGGAATgtggtaaaaataaccgacaac                                        >  SRR19973078.1898356/1‑129 (MQ=60)
                                                                                                          GCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTtttggtttttcgggtttttgccagcattcataacaaaaatgcccgggcattttgtaaaaataatcgaaacc                                        >  SRR19973078.2728474/1‑79 (MQ=60)
                                                                                                           AATTTGCAACTGTCGGCGCTTTTCCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCG                                       <  SRR19973078.3584872/150‑1 (MQ=60)
                                                                                                                  AAGTGTCGGCGCTTTTCCAGTATTTTGCTAAAGATTTTATGAAAAACGGCGTGCGGGCCTTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCTTTCAGACGAAAATTGCCCGTGAATTGTGAAAAAATACGCGACAGCGCGCAATA                                <  SRR19973078.2728687/150‑1 (MQ=60)
                                                                                                                  AACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTgttttttgttttgtttttttggattttttaccacagaaaaaaccaaaaataccccgacaagtgaaaaaaaaaaacacaaccccccaaa                                >  SRR19973078.3765219/1‑62 (MQ=60)
                                                                                                                  AACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTttttttttttgtttttttgggtttttcccctcaataattagaaaaattcacgggaattttgtaaaaataatagaaacagccccaaa                                >  SRR19973078.712429/1‑64 (MQ=60)
                                                                                                                  AACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTTGGTTTTTCGCTTTTTTACCAGCAATAAAACGAAAAATGCCCGGGAAATGTTAAAAAATAACCGACAGCCCGCAATA                                >  SRR19973078.712423/1‑150 (MQ=53)
                                                                                                                  AACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTttgttggtttttggctttttgtcccgcaataacaagaaaaaatccccgggaatttgtaaaaaaaagcgacaccgcccaaaa                                >  SRR19973078.4199429/1‑69 (MQ=60)
                                                                                                                  AACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAAGGCCTGCGGGCCGTTTTttgttttttgggtttttggcttttttgccaccaaagaaaacaaaaattgcccgggaatgtgtaaaaaaaaacaacaccgccccaaa                                >  SRR19973078.3400701/1‑64 (MQ=60)
                                                                                                                  AACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATA                                <  SRR19973078.3166477/150‑1 (MQ=60)
                                                                                                                  AACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATA                                <  SRR19973078.4306413/150‑1 (MQ=60)
                                                                                                                    CTGTCGGCGCTTTTCCATTTTGTTGCTAAAATTTTTAGCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAC                              <  SRR19973078.527696/150‑1 (MQ=60)
                                                                                                                          GCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGTGGATTTTTCGCTTTTTGgccaccaataagaccaaaaattcccgggaaattttaaaaaaaaaacgacaccgcgcaaaaaaacttca                        >  SRR19973078.4752232/1‑82 (MQ=60)
                                                                                                                           CGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttttgtgattttcgcttttttgccacccaaaagacgaaaaaagcccccgaatttggaaaaataaagcgacaccccgcaaaaaaccttata                       >  SRR19973078.244549/1‑60 (MQ=60)
                                                                                                                                     GTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAA             <  SRR19973078.106206/150‑1 (MQ=60)
                                                                                                                                      TATGTTGCTAACGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAA            <  SRR19973078.1918308/150‑1 (MQ=60)
                                                                                                                                       ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTtttttgttttttgggttttttggcagcatttattggaaaatttggcggggtatgtgttaaaattagagtcatgggggcaaaaacggtgtggtagagtaaaag           >  SRR19973078.4141748/1‑48 (MQ=60)
                                                                                                                                              TAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtggttttttgggttttttgccaccattaagaaggaaaaataccggggaatgtgtaaaaaaaagcgacaccgcccacaaaacaatataggaacaaaaaaattatgac    >  SRR19973078.3468294/1‑41 (MQ=59)
                                                                                                                                               AAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTtttttttttggtttttttgggttttggccaccaatcagaagaaaaatgccccgggagtttttaaaaaaaagccgcaccgcgcacaaaaccgtcacgaaacaaaaaactgatgccg   >  SRR19973078.3491527/1‑35 (MQ=60)
                                                                                                                                                AAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGGTTTTTCGCTTTTTtaccccccataagaccaaaaatgccccggcaatttgtaaaaaaaaaccacagcccgccaaaacccctcacgacaccaaaaagagattccgc  >  SRR19973078.3823797/1‑59 (MQ=56)
                                                                                                                                                  |                                                                                                                                                   
CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGC  >  CP009273/450144‑450437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑