Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA99.9% 1036.1 / ‑6.7 319intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (150/163);  minor base T (0/4);  total (150/169)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 321 total aligned reads displayed.

TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450143‑450436
                                                                                                                                                   |                                                                                                                                                     
tCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAt                                                                                                                                                     <  1:3513001/150‑1 (MQ=255)
    gcgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTa                                                                                                                                                 >  1:4764969/1‑150 (MQ=255)
     cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAt                                                                                                                                                <  2:4569001/150‑1 (MQ=255)
       cTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                              >  1:515882/1‑150 (MQ=255)
        tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaa                                                                                                                                             >  2:2177798/1‑150 (MQ=255)
          gCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaa                                                                                                                                           >  2:813161/1‑150 (MQ=255)
           ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                          <  2:126049/150‑1 (MQ=255)
            tctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAc                                                                                                                                         <  1:4024008/150‑1 (MQ=255)
               gCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                      >  1:4868669/1‑150 (MQ=255)
                 aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCt                                                                                                                                    >  2:4003184/1‑150 (MQ=255)
                  tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTg                                                                                                                                   >  1:2165920/1‑150 (MQ=255)
                   aCTTACACTATTAAGGTGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGc                                                                                                                                  <  1:3128286/150‑1 (MQ=255)
                     ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCgg                                                                                                                                >  1:4151247/1‑150 (MQ=255)
                        cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                             <  2:5185156/150‑1 (MQ=255)
                           tattatGGTGTGGGACGGGTTGCAGGACACCGTCTTTGCCTCTCCCCCCTGTCGTGGAGCTGGAAGCATCGCGTAAAAACGAATTGGCACCTGTCGGCGCTTTTCCTGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCgt‑t                                                                                                                         <  1:3722372/150‑1 (MQ=255)
                            aTTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tg                                                                                                                        >  1:5116789/1‑149 (MQ=255)
                               aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgt                                                                                                                     >  2:2487753/1‑150 (MQ=255)
                               aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgt                                                                                                                     >  1:854424/1‑150 (MQ=255)
                                 gtaGTGGGATGGGTTGCAGTACACCTTCTTGGCCTCTCCTCCCTGTCTTGGAGCAGGAAGCATCGCGTAAAAACTCATTTGAAACTGTCGGCGCTTTTCAAGTATGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCgt‑tttgttt                                                                                                                   <  1:902955/148‑1 (MQ=255)
                                  gAGGGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCACCGGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgt‑tttgtttt                                                                                                                  <  1:1836891/150‑1 (MQ=255)
                                   aGTGGTAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAAGGCCTGCGGGCCgt‑tttgttttt                                                                                                                 >  2:4352912/1‑149 (MQ=255)
                                   aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGt‑tttgttttg                                                                                                                 >  2:4233779/1‑150 (MQ=255)
                                   aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGt‑tttgttttg                                                                                                                 >  1:1303093/1‑150 (MQ=255)
                                    gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑tttgttttgt                                                                                                                >  2:1078810/1‑150 (MQ=255)
                                     tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTc                                                                                                               <  1:1614443/150‑1 (MQ=255)
                                      gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑tttgttttgtat                                                                                                              >  1:4378057/1‑148 (MQ=255)
                                        gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTGTCT‑gg                                                                                                           >  2:2936516/1‑150 (MQ=255)
                                         aaGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGa                                                                                                          >  1:3976157/1‑150 (MQ=255)
                                         aaGGGTAGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCAGT‑TTTTTTTTTTAT‑GGa                                                                                                          >  1:3458398/1‑150 (MQ=255)
                                          aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAt                                                                                                         <  2:106125/150‑1 (MQ=255)
                                            ggTTGCCGGACACCGTCTTGGCCTCACCTCCCTGTCGTGTAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCATTATGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAttt                                                                                                       <  1:4473986/150‑1 (MQ=255)
                                            ggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAttt                                                                                                       <  1:3932869/150‑1 (MQ=255)
                                             gTTGCAGGACACCGTCTTTGCCACTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGAtttt                                                                                                      <  1:4537941/150‑1 (MQ=255)
                                              ttGCTGGACACCGTGTTTGCCTCTCCTCCGTGTCGTGGAGCAGGAAGCATCTCGTATTTTGGCTTTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAATATTTTTTTGAAAATCGGCCTGCGTGCCGT‑TTTGTTTTGTCT‑GGATTTTg                                                                                                     <  2:1527707/150‑1 (MQ=255)
                                                gCAGGACACCTTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTgcg                                                                                                   <  1:2737727/150‑1 (MQ=255)
                                                gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTCT‑GGATTTTgcg                                                                                                   >  2:36303/1‑150 (MQ=255)
                                                 cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑GTTGTTTTGTGT‑GGGTTTTgggc                                                                                                  >  2:2377381/1‑150 (MQ=255)
                                                 cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCAG‑TTTGTTTTGTCT‑GGATTTTgcgc                                                                                                  >  1:3346605/1‑150 (MQ=255)
                                                        ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGc                                                                                           <  2:369385/150‑1 (MQ=255)
                                                        ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGGTTTTTGG‑GGTTTTTTGGCttttttg                                                                                           >  1:1523520/1‑148 (MQ=255)
                                                         cGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACGGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCG‑GGATTTTGCGCTTTTTGcc                                                                                          <  2:3189977/150‑1 (MQ=255)
                                                          gTCTTTGCCTCTCCTCGCTGTCGTGGTGCAGTAAGCATCGAGTAAAAACGATTTTGCAACTGTCGGCGCTTTTCCTTTTTTTTGCTAAAATTTTTTGGAAATACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         <  1:3216080/150‑1 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGTGGGTTTTTGGGGTTTTTGcc                                                                                          >  1:1106426/1‑150 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATGTTATGAAAAACGGCCTGCGGGCCGT‑TTGGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         >  2:735166/1‑150 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGT‑GGATTTTGCGCTTTTTGcac                                                                                         >  2:2017587/1‑148 (MQ=255)
                                                          gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGATTTTTCGCTTTTTTccc                                                                                         >  2:5327384/1‑150 (MQ=255)
                                                            cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAg                                                                                       <  2:409923/150‑1 (MQ=255)
                                                              ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCa                                                                                     >  2:4390102/1‑150 (MQ=255)
                                                                 ccTCTCCTCCCTGTCGTGGAGCTGGAAGCATCTCGTAAAAACGCATTTGCATCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTc                                                                                  <  2:4904850/150‑1 (MQ=255)
                                                                  ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                 >  2:2918707/1‑150 (MQ=255)
                                                                  ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGATTTTTCGCGTTTTGCCCAGccataa                                                                                 >  1:1215264/1‑145 (MQ=255)
                                                                           cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTAT‑GGATTTTTAGCTTTTTTGCCCGCAATAAG‑ACAaaaaa                                                                       >  1:2861520/1‑149 (MQ=255)
                                                                            tGTCGTGGAGCAGGTAGCATCGCGTAAAATCTCTTTTGCTCCTGTCGGGGCTTTTACAGTTTGTTGATAAATTTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTTCGCTTTTTGCCCAGCATTCAG‑ACGAAAAtt                                                                      <  1:3772083/150‑1 (MQ=255)
                                                                             gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTg                                                                     <  2:3371849/150‑1 (MQ=255)
                                                                                gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGccc                                                                  <  1:665903/150‑1 (MQ=255)
                                                                                  ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCgg                                                                >  2:3430372/1‑150 (MQ=255)
                                                                                      cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTGT‑GGATTTTTAGCTTTTTTCCCAGCATTCAG‑ACGAAAATTTCCCGGGAAt                                                            >  2:3284037/1‑150 (MQ=255)
                                                                                      cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAAt                                                            <  2:5083197/150‑1 (MQ=255)
                                                                                      cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTAAG‑ACGAAAATTGCCCGGGAAt                                                            >  2:3798660/1‑150 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAAtt                                                           <  2:1720132/150‑1 (MQ=255)
                                                                                       aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGTTTTTTGGCTTTTTTGCCAGCAATGAG‑TCGGAAATTGGCCggggagt                                                           >  2:4467123/1‑146 (MQ=255)
                                                                                        ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTTTGT‑GGGTTTTTCGCTTTTTTCCCACCATTAAG‑TCGAAAATTTACCGGGAAttt                                                          >  1:792534/1‑149 (MQ=255)
                                                                                        ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGGTTTTGCGCTTTTTTCCCAGCATTACT‑CAGAAAAATGCCCggggagtg                                                          >  2:4027222/1‑145 (MQ=255)
                                                                                         gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGT‑GGGTTTTGGGGTTTTTTGCCCGCAATAAGAACAAAAATTGCCGGGGGATtg                                                          >  1:1004064/1‑150 (MQ=255)
                                                                                          aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATtgtg                                                        <  2:5097530/150‑1 (MQ=255)
                                                                                           aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAATCGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGa                                                       >  2:2641175/1‑150 (MQ=255)
                                                                                           aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGACTTGTGa                                                       >  2:2770670/1‑150 (MQ=255)
                                                                                            gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGaa                                                      <  2:3850716/150‑1 (MQ=255)
                                                                                              aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGaaaa                                                    >  2:3478838/1‑150 (MQ=255)
                                                                                                 gcttAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                 <  1:4107832/147‑1 (MQ=255)
                                                                                                 gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                 <  1:507173/150‑1 (MQ=255)
                                                                                                 gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑GTTGTTTTTTGG‑GGTTTTTTGGCTTTTTGCCCAGCCATCAG‑ACGAAAATTGGCCGGGGAGTGTGAAAAAAt                                                 >  1:341689/1‑150 (MQ=255)
                                                                                                  cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGAGCTTTTTGCCCAGCAATAAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATa                                                >  1:1720124/1‑150 (MQ=255)
                                                                                                   gTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATAc                                               <  1:3924830/150‑1 (MQ=255)
                                                                                                   gTAAAAACGATTTTGCAACTGTCGGCGCTTTTCCTGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATAc                                               <  1:2297226/150‑1 (MQ=255)
                                                                                                      aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                            <  1:5281834/150‑1 (MQ=255)
                                                                                                       aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑TGTGTTTTTTGT‑GGATTTTTGGCTTTTTGGCCAGCAATCAG‑ACGAAAAATGGCCGGGAAATTTGGAAAAATAcgcgg                                           >  2:1359318/1‑149 (MQ=255)
                                                                                                        aaCGCATTTGCAACTGTCGGCGCTTGTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGAc                                          <  2:3866452/150‑1 (MQ=255)
                                                                                                            cATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGT‑GGTTTTTTGGCTTTTTGCCCCGCAATCAG‑TCGAAAAATTGCCGGGAAGTGTGTAAAAATACACGAAAgcg                                      >  2:4509588/1‑150 (MQ=255)
                                                                                                              tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                    <  2:4764969/150‑1 (MQ=255)
                                                                                                                  cAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGAG‑GGATTTTGGGTTTTTTGCCCACCATAAAA‑ACAAAAATTG‑CCGGGGATTTTTAAAAAATAAACCACAgggcgccca                                >  1:569648/1‑147 (MQ=255)
                                                                                                                   aagtgtgGGCGATTTTCCAGTATTTTGATAAAAATTTTATGAAACACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGAATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                               <  1:4090445/147‑1 (MQ=255)
                                                                                                                   aaCTGTCGGCGATTTTCCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGAGCGCAATa                               <  2:4091603/150‑1 (MQ=255)
                                                                                                                         gggCGATTTTCCTGTATTTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCTT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                         <  1:3662335/149‑1 (MQ=255)
                                                                                                                           gctcTTTTCAATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCAAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                       <  1:4290005/147‑1 (MQ=255)
                                                                                                                           gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                       <  2:3946956/150‑1 (MQ=255)
                                                                                                                            cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtctc                      <  2:4631979/150‑1 (MQ=255)
                                                                                                                             gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                     <  2:747290/150‑1 (MQ=255)
                                                                                                                             gcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                     <  2:835881/150‑1 (MQ=255)
                                                                                                                               ttttCCAGTATGTTGCTAAAAATTTTATGTAAATAGGCCTGCTGGCCTT‑TTTTTTTTGTTT‑GGATTTTGAGCTTTTTGCCCAGCATTCAG‑ACGAAATTTGCCCGGTATTTGTGAAAAATTTCGCGACAGCGCGCAATAACCGTTCTCGag                   <  2:647069/150‑2 (MQ=255)
                                                                                                                               ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                   <  2:1868422/150‑1 (MQ=255)
                                                                                                                                   ccAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAt               <  2:4952446/150‑1 (MQ=255)
                                                                                                                                          gtttatAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg        <  1:1937179/145‑1 (MQ=255)
                                                                                                                                          gTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTg        <  2:1815886/150‑1 (MQ=255)
                                                                                                                                           ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa       <  2:4868669/150‑1 (MQ=255)
                                                                                                                                            tGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt      <  1:1078810/150‑1 (MQ=255)
                                                                                                                                            tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt      <  2:2519498/150‑1 (MQ=255)
                                                                                                                                            tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt      <  1:239558/150‑1 (MQ=255)
                                                                                                                                              cTAAAAATTTTTTGAAAAACGGCGGGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGATTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc    <  1:2263224/150‑1 (MQ=255)
                                                                                                                                                aaaaATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCg  <  1:3085557/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑ACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG  >  CP009273/450143‑450436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTA  >  CP009273/450141‑450439
                                                                                                                                                     |                                                                                                                                                      
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                        >  SRR19973062.2532942/1‑150 (MQ=60)
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                        >  SRR19973062.2532963/1‑150 (MQ=60)
  TCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAT                                                                                                                                                      <  SRR19973062.3530805/150‑1 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                     >  SRR19973062.4460497/1‑150 (MQ=60)
     TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTT                                                                                                                                                   >  SRR19973062.1048402/1‑150 (MQ=60)
     TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTT                                                                                                                                                   >  SRR19973062.2936281/1‑150 (MQ=60)
      GCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTA                                                                                                                                                  >  SRR19973062.4789739/1‑150 (MQ=60)
         CTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGA                                                                                                                                               >  SRR19973062.518304/1‑150 (MQ=60)
          TGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAA                                                                                                                                              <  SRR19973062.337999/150‑1 (MQ=60)
           GGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAA                                                                                                                                             >  SRR19973062.550392/1‑150 (MQ=60)
              TCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAC                                                                                                                                          <  SRR19973062.4044421/150‑1 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                         >  SRR19973062.3176798/1‑150 (MQ=60)
               CTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACG                                                                                                                                         <  SRR19973062.2279146/150‑1 (MQ=60)
                 GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                       >  SRR19973062.4893767/1‑150 (MQ=60)
                 GCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGC                                                                                                                                       >  SRR19973062.4893877/1‑150 (MQ=60)
                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                     <  SRR19973062.5058797/150‑1 (MQ=60)
                   ATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCT                                                                                                                                     >  SRR19973062.962596/1‑150 (MQ=60)
                    TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTG                                                                                                                                    >  SRR19973062.2176865/1‑150 (MQ=60)
                    TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTG                                                                                                                                    >  SRR19973062.839531/1‑150 (MQ=60)
                    TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTG                                                                                                                                    >  SRR19973062.4147959/1‑150 (MQ=60)
                     ACTTACACTATTAAGGTGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                                   <  SRR19973062.3144384/150‑1 (MQ=60)
                     ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                                   >  SRR19973062.3990346/1‑150 (MQ=60)
                       TTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGG                                                                                                                                 >  SRR19973062.4172770/1‑150 (MQ=60)
                          CACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCC                                                                                                                              >  SRR19973062.1565324/1‑150 (MQ=60)
                          CACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCC                                                                                                                              >  SRR19973062.1565325/1‑150 (MQ=60)
                           ACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCG                                                                                                                             >  SRR19973062.1634005/1‑150 (MQ=60)
                             TATTATGGTGTGGGACGGGTTGCAGGACACCGTCTTTGCCTCTCCCCCCTGTCGTGGAGCTGGAAGCATCGCGTAAAAACGAATTGGCACCTGTCGGCGCTTTTCCTGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                           <  SRR19973062.3741474/150‑1 (MQ=60)
                             TATTAAGGTGTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                           <  SRR19973062.3741578/150‑1 (MQ=60)
                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTT                                                                                                                           <  SRR19973062.3741587/150‑1 (MQ=60)
                              ATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTG                                                                                                                          >  SRR19973062.5143866/1‑150 (MQ=60)
                               TTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTT                                                                                                                         <  SRR19973062.421580/150‑1 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG                                                                                                                        <  SRR19973062.3321839/150‑1 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttg                                                                                                                        >  SRR19973062.4076821/1‑145 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG                                                                                                                        <  SRR19973062.3321825/150‑1 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACCGTATGTTGCTAAAAATTTTATGAAAAACGTCCTGCGGGCCGggttg                                                                                                                        >  SRR19973062.4112676/1‑145 (MQ=60)
                                TAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTACAGGATGTTGCTAAAAATTTTATTAAAAAagtccttcgggccgtggtg                                                                                                                        >  SRR19973062.1818269/1‑131 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGT                                                                                                                       >  SRR19973062.4397779/1‑150 (MQ=60)
                                 AAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGT                                                                                                                       >  SRR19973062.858111/1‑150 (MQ=60)
                                   GTAGTGGGATGGGTTGCAGTACACCTTCTTGGCCTCTCCTCCCTGTCTTGGAGCAGGAAGCATCGCGTAAAAACTCATTTGAAACTGTCGGCGCTTTTCAAGTATGTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTT                                                                                                                     <  SRR19973062.906842/150‑1 (MQ=60)
                                    GAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                    <  SRR19973062.1021302/150‑1 (MQ=60)
                                    GAGGGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCACCGGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTT                                                                                                                    <  SRR19973062.1846018/150‑1 (MQ=60)
                                     AGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTT                                                                                                                   >  SRR19973062.1309056/1‑150 (MQ=60)
                                      GTGGGAAGGGTTGCCGCACACCCTCATTGCCGCTCCTCCCCGTCGTGGAGCCCGAAGCATGGCGTAAAACCGCATTGGCAACTGTCGGCGCTTGCCCAGTATGTTGCTAAAATTTTTTTGAAATACGGCCTGCGGGCCGTTTTGTTTTGT                                                                                                                  <  SRR19973062.1524664/150‑1 (MQ=60)
                                      GTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTGT                                                                                                                  >  SRR19973062.3927224/1‑150 (MQ=60)
                                       TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTC                                                                                                                 <  SRR19973062.1622435/150‑1 (MQ=60)
                                        GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTAT                                                                                                                >  SRR19973062.4400512/1‑150 (MQ=60)
                                         GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTG                                                                                                               >  SRR19973062.3515821/1‑150 (MQ=60)
                                           AAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCTGGA                                                                                                             >  SRR19973062.3996263/1‑150 (MQ=60)
                                           AAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGA                                                                                                             >  SRR19973062.3996289/1‑150 (MQ=60)
                                           AAGGGTAGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCAGTTTTTTTTTTTATGGA                                                                                                             >  SRR19973062.3476025/1‑150 (MQ=60)
                                              GGTTGCCGGACACCGTCTTGGCCTCACCTCCCTGTCGTGTAGCAGGAAGCATCGCGTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCATTATGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTT                                                                                                          <  SRR19973062.4496904/150‑1 (MQ=60)
                                              GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTT                                                                                                          <  SRR19973062.3952852/150‑1 (MQ=60)
                                               GTTGCAGGACACCGTCTTTGCCACTCCGCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTT                                                                                                         <  SRR19973062.4561585/150‑1 (MQ=60)
                                                  GCAGGACACCTTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCG                                                                                                      <  SRR19973062.2752159/150‑1 (MQ=60)
                                                  GCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttttttttgttgttttttgg                                                                                                      >  SRR19973062.3231002/1‑128 (MQ=60)
                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtggttttttggg                                                                                                     >  SRR19973062.1443651/1‑135 (MQ=60)
                                                   CAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCAGTTTGTTTTGTCTGGATTTTGCGC                                                                                                     >  SRR19973062.3363858/1‑150 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTTTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTT                                                                                                   <  SRR19973062.4014100/150‑1 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgtggttttttggctt                                                                                                   >  SRR19973062.3910126/1‑133 (MQ=60)
                                                      GACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTT                                                                                                  <  SRR19973062.4868230/150‑1 (MQ=60)
                                                      GACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgttgttttttgggttt                                                                                                  >  SRR19973062.1932476/1‑132 (MQ=60)
                                                       ACACCGTCTGGGCCTCCCCCCCCTGGCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCACCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTT                                                                                                 <  SRR19973062.2287724/150‑1 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgttgttttttggcgtttt                                                                                                >  SRR19973062.1870970/1‑122 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGTAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCTTTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                               <  SRR19973062.5118920/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtttgttttgtgggtttttggggttttt                                                                                               >  SRR19973062.1822670/1‑120 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                               <  SRR19973062.216711/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                               <  SRR19973062.2178391/150‑1 (MQ=60)
                                                          CCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttggtttttggggttttttggcttttttg                                                                                              >  SRR19973062.1531028/1‑120 (MQ=60)
                                                            GTCTTTGCCTCTCCTCGCTGTCGTGGTGCAGTAAGCATCGAGTAAAAACGATTTTGCAACTGTCGGCGCTTTTCCTTTTTTTTGCTAAAATTTTTTGGAAATACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCC                                                                                            <  SRR19973062.3232780/150‑1 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTCTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTgtgggtttttggggtttttgcc                                                                                            >  SRR19973062.1111628/1‑128 (MQ=60)
                                                            GTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTTTGTGGTTTTTGGGCTTTTTGGCC                                                                                            >  SRR19973062.1111466/1‑150 (MQ=60)
                                                             TCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                           <  SRR19973062.491414/150‑1 (MQ=60)
                                                              CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGGGCTTTTTGCCCAG                                                                                          >  SRR19973062.897854/1‑150 (MQ=60)
                                                                TTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgttgtgttttggcttttttcccagca                                                                                        >  SRR19973062.5205868/1‑122 (MQ=60)
                                                                  GCCTCTCCTCCCTGTCTTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT                                                                                      <  SRR19973062.4619621/150‑1 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCACCTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTC                                                                                     <  SRR19973062.4526786/150‑1 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTC                                                                                     <  SRR19973062.4526635/150‑1 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCAATC                                                                                     >  SRR19973062.3453060/1‑150 (MQ=60)
                                                                   CCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGTTTTTGCGCTTTTTGgccacccttc                                                                                     >  SRR19973062.3452835/1‑140 (MQ=60)
                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTTTTTTGTATGGTTTTTGAGCATTTTGGCCAGCAataa                                                                                    >  SRR19973062.4022350/1‑146 (MQ=60)
                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGTGGATTTTTCGCGTTTTGCCCAGCcataa                                                                                    >  SRR19973062.1220919/1‑145 (MQ=60)
                                                                     TCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAATTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTttttgtttttttgggttatttggcaccaataag                                                                                   >  SRR19973062.4079162/1‑117 (MQ=60)
                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCAATTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAC                                                                                 <  SRR19973062.563583/150‑1 (MQ=60)
                                                                        CCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttggtttgtgggggggttgggggtttttgccagccataagaac                                                                                >  SRR19973062.1475951/1‑105 (MQ=60)
                                                                         CTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttttgggttttgggggtttttgccccccataagaaga                                                                               >  SRR19973062.1836831/1‑105 (MQ=60)
                                                                          TCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGATTTTTGGCTTTTTTCCCAGCAATAAGACG‑AA                                                                             >  SRR19973062.124282/1‑150 (MQ=60)
                                                                           CCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAA                                                                            <  SRR19973062.2856334/150‑1 (MQ=60)
                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGATTTTTCGCTTTTTTCCCAGCATTAAGAAG‑AAAAA                                                                          >  SRR19973062.5298335/1‑150 (MQ=60)
                                                                             CTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATGGATTTTtagcttttttgcccgcaataagacaaaaaa                                                                           >  SRR19973062.2876648/1‑120 (MQ=60)
                                                                              TGTCGTGGAGCAGGTAGCATCGCGTAAAATCTCTTTTGCTCCTGTCGGGGCTTTTACAGTTTGTTGATAAATTTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGACG‑AAAATT                                                                         <  SRR19973062.3791406/150‑1 (MQ=60)
                                                                              TGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATT                                                                         <  SRR19973062.4820006/150‑1 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                     <  SRR19973062.668978/150‑1 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                     <  SRR19973062.4349165/150‑1 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCC                                                                     <  SRR19973062.669003/150‑1 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCAGGATTTTGCGCTTTTTTCCCAGCAATCAGACG‑AAAATTTGCCGG                                                                   >  SRR19973062.895150/1‑150 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAT                                                               <  SRR19973062.2843663/150‑1 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGGGTTTTTTGTGGGTTTTGGGGTTTTTGCCCAGCAATCAGACG‑AAAATTTCCCGGGTATT                                                              >  SRR19973062.403596/1‑150 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTAGGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATT                                                              <  SRR19973062.604457/150‑1 (MQ=60)
                                                                                          GGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTGGGTTTTTCGCTTTTTTCCCACCATTAAGTCG‑AAAATTTACCGGGAATTT                                                             >  SRR19973062.796056/1‑150 (MQ=60)
                                                                                          GGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTTTTTTTTGTGGATTTTGgggttttttgcccgccttcagacgaaaatttaccgggaagtt                                                              >  SRR19973062.2950363/1‑108 (MQ=60)
                                                                                          GGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTG                                                             <  SRR19973062.3112126/150‑1 (MQ=60)
                                                                                           GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggttttggggttttttgcccgcaataagaacaaaaattgccgggggattg                                                             >  SRR19973062.1008748/1‑95 (MQ=60)
                                                                                           GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTgtggttttttttggtgttggggggtttggccccccgtggctaggaaaattgcaggggaattg                                                             >  SRR19973062.1449975/1‑88 (MQ=60)
                                                                                           GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttttggttttttggggtttttgcccgcaataagaaaaaaatttgcccggggttgt                                                             >  SRR19973062.1449982/1‑95 (MQ=60)
                                                                                           GAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCGGCGGGCCGTggtggtttttgtggggttttgggtgttttggcacccatggggcggaaaattgccgggggtttt                                                             >  SRR19973062.1449950/1‑87 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgggggtttttggggtttttgccccccagaagaccgaaaatggcccgggcatgtg                                                            >  SRR19973062.2460370/1‑85 (MQ=60)
                                                                                            AAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTTTGTGGGTTTTGGGCTTTTTTGCCAGCAATCAGACG‑AAAATTTCCCGGGGATTGTG                                                           >  SRR19973062.3944851/1‑150 (MQ=60)
                                                                                            AAGCATCGCGAAAAAACGCATTTGCAACTGACGGCGCTTTTCCAGAATGTTGCTAAAAATTATATGAAAAAAGGGCTGCGGGggatgttttgttttgtatagttttaggttttttgacaaccataagagaaaaaattgccgggaaattgt                                                            >  SRR19973062.2460363/1‑82 (MQ=55)
                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggtttttgggttttttgccagcaataacaccaaaatttcccgggacaggtggtaa                                                        >  SRR19973062.4242230/1‑90 (MQ=60)
                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTttgtgggttttgggcgtttttgccagcaatgagtcgcaaatttcccggggagtgtgtaaa                                                        >  SRR19973062.4262246/1‑90 (MQ=60)
                                                                                                  CGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttttgggtttttggcttttttgccagcaataatacgaaaaattgccgggaagttttttaaaa                                                      >  SRR19973062.3253093/1‑80 (MQ=60)
                                                                                                  CGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAA                                                     >  SRR19973062.411334/1‑150 (MQ=60)
                                                                                                   TCGTAATAACCCATTTGAAACTGTCGGCGCTTTTCCTGTATGTTGATAAAAATTTTTTGAAAATCGGCCTGCGGGCCGTTTTGTATTGTCTGGTTTTTGCGCTTTTTGACCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAAT                                                    <  SRR19973062.3201201/150‑1 (MQ=60)
                                                                                                   GCTTAAAAACGAATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAAT                                                    <  SRR19973062.4129048/150‑1 (MQ=60)
                                                                                                   GCGTAAAAACGCATTTGCAACTTTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAAT                                                    <  SRR19973062.4129470/150‑1 (MQ=60)
                                                                                                   GCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAAT                                                    <  SRR19973062.509544/150‑1 (MQ=60)
                                                                                                   GCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAAT                                                    <  SRR19973062.1103625/150‑1 (MQ=60)
                                                                                                   GCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTGTTTTTTGGGGTTTTTTGGCTTTTTGCCCAGCCATCAGACG‑AAAATTGGCCGGGGAGTGTGAAAAAAT                                                    >  SRR19973062.343261/1‑150 (MQ=60)
                                                                                                    CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgttttttgttggttttgggggtttttggccccagtaagacgaatatttgcccggaagtgtggaaaaata                                                    >  SRR19973062.531353/1‑77 (MQ=60)
                                                                                                    CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTgtgggattttgggttttttgccagcaataagaagaaaaattgccggggattgtgtaaaaata                                                    >  SRR19973062.1232923/1‑88 (MQ=60)
                                                                                                    CGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGAGCTTTTTGCCCAGCAATAAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATA                                                   >  SRR19973062.1728517/1‑150 (MQ=60)
                                                                                                     TTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                                  <  SRR19973062.3720648/150‑1 (MQ=60)
                                                                                                     GTAAAAACTCATTTGCAACTGTCGGCGATTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                                  <  SRR19973062.3720598/150‑1 (MQ=60)
                                                                                                     GTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                                  <  SRR19973062.3944787/150‑1 (MQ=60)
                                                                                                     GTAAAAACGATTTTGCAACTGTCGGCGCTTTTCCTGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAC                                                  <  SRR19973062.2309169/150‑1 (MQ=60)
                                                                                                      TAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgtggtgttttgggtttttggcccgccagaagacgtaaatttggcgggggatgtttaaaaataag                                                  >  SRR19973062.1499562/1‑75 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                               <  SRR19973062.5309605/150‑1 (MQ=60)
                                                                                                        AAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCG                                               <  SRR19973062.357960/150‑1 (MQ=60)
                                                                                                         AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtgtttttttgtgggtttttggggttttggccagcattcagaccaaaaattgccggggattgtggaaaaataagcgc                                               >  SRR19973062.1189009/1‑73 (MQ=60)
                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGAC                                             <  SRR19973062.3109694/150‑1 (MQ=60)
                                                                                                                  TGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCA                                     <  SRR19973062.2490262/150‑1 (MQ=60)
                                                                                                                  TGCAACTGTCGGCGATTTTCCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCA                                     <  SRR19973062.2490295/150‑1 (MQ=60)
                                                                                                                    CAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggtttttttttgtttgtttttagggtttttgaccaccataagaacaaaaaatcccccggaaatttaaaaaaaaaaagcacacccgccca                                    >  SRR19973062.1907477/1‑61 (MQ=60)
                                                                                                                    CAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgagggattttgggttttttgcccaccataaaaacaaaaattgccggggatttttaaaaaataaaccacagggcgccca                                    >  SRR19973062.572322/1‑62 (MQ=60)
                                                                                                                    CAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTttgtggttttttggctattttaccagcaaaaaaacgaaaaatgaccccgcagtttgaaaaaaaaagacacaccgcccaaa                                    >  SRR19973062.2882157/1‑70 (MQ=60)
                                                                                                                     AAGTGTGGGCGATTTTCCAGTATTTTGATAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATA                                  <  SRR19973062.4111504/150‑1 (MQ=60)
                                                                                                                      AGTGTCGGCGCTTTTCATTTATGTTGATAAATTTTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAA                                 <  SRR19973062.36559/150‑1 (MQ=60)
                                                                                                                      ACTGTCGGCGCTTTTCCAGTATGTTTCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTtttttttattgttttttggagtttttcccagcaatgaaaagaaaaattcacgggaaatttttaaaaaaaagccaaaaaccgcaaaaa                                  >  SRR19973062.3037206/1‑63 (MQ=60)
                                                                                                                      ACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAA                                 <  SRR19973062.4617255/150‑1 (MQ=60)
                                                                                                                       ggGTCGGCGCTTTTCCAGTTGGTTGAAAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAC                                <  SRR19973062.3151891/148‑1 (MQ=60)
                                                                                                                        TTTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACC                               <  SRR19973062.3507428/150‑1 (MQ=60)
                                                                                                                         GTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTATTGATTTTGCGCTTTTTTCCCAGCAaaaagacccaaattgccccggcaatttgtaaaaaaaagcgaccccccccaaaaaccc                               >  SRR19973062.800286/1‑93 (MQ=60)
                                                                                                                           GGGCGATTTTCCTGTATTTTGATAAAAATTTTATTAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTT                            <  SRR19973062.3681116/150‑1 (MQ=60)
                                                                                                                           CGGCTCTTTTAAAGTATGTTTATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTT                            <  SRR19973062.738559/150‑1 (MQ=60)
                                                                                                                             GCTCTTTTCAATTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                          <  SRR19973062.4312175/150‑1 (MQ=60)
                                                                                                                             GCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                          <  SRR19973062.5153564/150‑1 (MQ=60)
                                                                                                                             GCGCTTTTCCAGTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                          <  SRR19973062.5153616/150‑1 (MQ=60)
                                                                                                                             GCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTGTTTTTTCTGGGTTTTGAGCTTTTTGCCCCGCAATCAGACG‑AAAATTGCCCGGGAATTTTGAAAAAATAAGCGACAGCGCGCAAAAACCGTTCT                          >  SRR19973062.826726/1‑150 (MQ=60)
                                                                                                                             GCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAATCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                          <  SRR19973062.1709609/150‑1 (MQ=60)
                                                                                                                             GCGCTTTCCCTGTTTGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCGGTTTTGTTTTGTCTGGTTTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                          <  SRR19973062.1857558/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCCAGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                         <  SRR19973062.5131701/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCCAGTATTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                         <  SRR19973062.1209255/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                         <  SRR19973062.4479589/150‑1 (MQ=60)
                                                                                                                              CGCTTTGCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                         <  SRR19973062.2361/150‑1 (MQ=60)
                                                                                                                                     CCATTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAT                  <  SRR19973062.1919968/150‑1 (MQ=60)
                                                                                                                                     CCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCAT                  <  SRR19973062.2808074/150‑1 (MQ=60)
                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtggttttttggcttttttgccagcaataagaccaaaaattaccggggattgtgaaaaaataaacgacagcgcgcccaaacccataaagacacaaa                  >  SRR19973062.3867575/1‑44 (MQ=60)
                                                                                                                                      CAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgtggtttttgggcattttggccagcattcataagaaaaaatgccgggaattttgtaaaaataagccacagcccgcaaaaacccgtatagacacata                  >  SRR19973062.1183984/1‑44 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttggttttttggttttttgccccccaaaaaaagaaaaattacccggcaatttttaaaaaaaaaagacaacgcccaaaaaaccgtaaagaaaaaaaa                 >  SRR19973062.3595327/1‑43 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTtggcatttttcccaccaataagaccaaaaattccccggaattgtgaaaaaataaacgacagcccgccaaaaaccaaatagacacaaaa                 >  SRR19973062.675885/1‑62 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTGTCTTGTTTTTTCGCTTTTTTGCCAGCAATCAGACGAAAAAATGCCC‑GGACTTGTGAAAAAATAAGCGACAGCGCGCAcaaacccttatagaatcaaaa                >  SRR19973062.2179150/1‑129 (MQ=49)
                                                                                                                                          ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAG             <  SRR19973062.3682594/150‑1 (MQ=60)
                                                                                                                                           TGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtggtttttggggtttttggggtttttgtccagcaaacagacgaaaaaagccccggcaatggggaaaaaaaagccacagcccccccaaacccggcgacgcacaaaaaaat             >  SRR19973062.1955782/1‑39 (MQ=60)
                                                                                                                                            GTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTG           <  SRR19973062.1947198/150‑1 (MQ=60)
                                                                                                                                            GTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTG           <  SRR19973062.4376280/150‑1 (MQ=60)
                                                                                                                                              TGCTAAATATTTTATTAAAACCGGCCTTCGGGCCGTTTTGTTTTGTCTGTATTTTGCTCTTTTTGCCCATATTTCAGACG‑AAAATTGCCCGTGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAATAGTGAT         <  SRR19973062.3422209/150‑1 (MQ=60)
                                                                                                                                              TGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAAGTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAT         <  SRR19973062.1083895/150‑1 (MQ=60)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAT         <  SRR19973062.3422229/150‑1 (MQ=60)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAT         <  SRR19973062.240781/150‑1 (MQ=60)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAT         <  SRR19973062.240784/150‑1 (MQ=60)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGTGGATTTTGGGCTTTTTTCCCCGCAATAAGACG‑AAAAATTCCCGGGAATTGTGAAAAAATAAGGAACATCGCGCAAAAAACGTTCTAGACTCAAAAAAGTGAT         >  SRR19973062.134049/1‑150 (MQ=45)
                                                                                                                                               GCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATG        <  SRR19973062.2124209/150‑1 (MQ=60)
                                                                                                                                                CTAAAAATTTTTTGAAAAACGGCGGGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGATTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC       <  SRR19973062.2274675/150‑1 (MQ=60)
                                                                                                                                                CTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC       <  SRR19973062.3950065/150‑1 (MQ=60)
                                                                                                                                                  AAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCG     <  SRR19973062.3101506/150‑1 (MQ=60)
                                                                                                                                                     AATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTTAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTA  <  SRR19973062.3648764/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTA  >  CP009273/450141‑450439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑