Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 679.5 / NA 205intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (98/107);  total (98/107)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 206 total aligned reads displayed.

ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450145‑450434
                                                                                                                                                 |                                                                                                                                                    
 tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   >  1:450225/1‑148 (MQ=255)
  gcgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt                                                                                                                                                  >  1:2655234/1‑148 (MQ=255)
   cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                 <  1:1034257/148‑1 (MQ=255)
      tGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATg                                                                                                                                              >  2:877436/1‑148 (MQ=255)
           ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         <  2:2807377/148‑1 (MQ=255)
                tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    >  1:73572/1‑148 (MQ=255)
                     acacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCgg                                                                                                                               >  2:69341/1‑148 (MQ=255)
                      cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGAAACTGTCGGCGATTTTCAAGTATGTTGATAAAAATTTTATCAAAAACGGCCTGCggg                                                                                                                              <  1:1221209/148‑1 (MQ=255)
                      cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCggg                                                                                                                              >  2:345662/1‑148 (MQ=255)
                       acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                             <  2:2276433/148‑1 (MQ=255)
                        cTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                            >  2:1652989/1‑148 (MQ=255)
                           ttAAGGAGTGGGACGGGTGGCAGGCCACCGTCTTGGCCCCCCCGCCCTGTCGTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         <  1:1907863/148‑1 (MQ=255)
                           ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         >  1:3537178/1‑148 (MQ=255)
                           ttAAGGAGTGGGAAGGGTAGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         >  2:2630758/1‑148 (MQ=255)
                              aGGAGTGGGACGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                      <  1:1618245/148‑1 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑t                                                                                                                    <  2:2620310/148‑1 (MQ=255)
                               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑t                                                                                                                    <  1:3540415/148‑1 (MQ=255)
                                  gTGGGATGGGTTGCAGGACACCGTCTTTGCCTCTCCGCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tttt                                                                                                                 <  2:1899807/148‑1 (MQ=255)
                                  gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑tttt                                                                                                                 <  2:1103042/148‑1 (MQ=255)
                                     ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg‑ttttttg                                                                                                              >  1:1450983/1‑145 (MQ=255)
                                      gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTTTCt                                                                                                             >  2:420809/1‑148 (MQ=255)
                                      gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGggtg‑ttttgtgt                                                                                                             >  1:3441365/1‑146 (MQ=255)
                                         gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTG‑TTTTGTGTggg                                                                                                          >  1:26948/1‑147 (MQ=255)
                                           gTTGCAGCACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑Att                                                                                                       <  1:3354128/148‑1 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑Attt                                                                                                      >  2:2245524/1‑148 (MQ=255)
                                            ttGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGGTG‑TTTTTTGTggtgtt                                                                                                       >  1:1662890/1‑144 (MQ=255)
                                              gCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTg                                                                                                    <  2:1151206/148‑1 (MQ=255)
                                               cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑GTTTGTCTGG‑ATTTTgc                                                                                                   >  2:3171870/1‑148 (MQ=255)
                                                 ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTgcgc                                                                                                 <  1:685142/148‑1 (MQ=255)
                                                 ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTgcgc                                                                                                 <  2:1562607/148‑1 (MQ=255)
                                                   acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑tt                                                                                              <  2:958935/148‑1 (MQ=255)
                                                   acacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTTTGTTG‑TTTTTGGGC‑tt                                                                                              >  1:2245993/1‑148 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑ttt                                                                                             <  1:1745894/148‑1 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑ttt                                                                                             <  1:1714874/148‑1 (MQ=255)
                                                    cacCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTTTGTGG‑CTTTTtcgggtt                                                                                              >  2:2991525/1‑144 (MQ=255)
                                                     acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑tttt                                                                                            <  1:2337251/148‑1 (MQ=255)
                                                     acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTT‑TTTTGTGTGG‑GTTTTgggggttt                                                                                             >  2:1438/1‑141 (MQ=255)
                                                         tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGcc                                                                                        <  1:2364287/148‑1 (MQ=255)
                                                            ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTGG‑GTTTGTGTGG‑TTTTTGCGC‑TTTTTGaccct                                                                                     >  2:799636/1‑146 (MQ=255)
                                                             tGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGc                                                                                    <  1:2971860/148‑1 (MQ=255)
                                                               ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTGTGG‑ATTTTTCGC‑TTTTTGCCCCGCAt                                                                                  >  2:1697761/1‑148 (MQ=255)
                                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCAtt                                                                                 <  2:785804/148‑1 (MQ=255)
                                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCAtt                                                                                 <  1:1538881/148‑1 (MQ=255)
                                                                  ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGGTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGTCCAGCATTCa                                                                               >  2:3274018/1‑148 (MQ=255)
                                                                   tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAg                                                                              <  2:3443573/148‑1 (MQ=255)
                                                                    ccccccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAAGTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAGGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGa                                                                             <  1:929649/145‑1 (MQ=255)
                                                                      tccCTGTCGTGTATCATGAAGCATAGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTACCAGTATGTTGCTAAAATTTTTTTGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACg                                                                           <  1:1404803/148‑1 (MQ=255)
                                                                      tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACg                                                                           <  2:190592/148‑1 (MQ=255)
                                                                       cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑a                                                                         <  1:3054546/148‑1 (MQ=255)
                                                                        ccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTGTGG‑ATTTTGCGC‑TGTTTGCCCAGCATTCAGACG‑aa                                                                        >  2:2444407/1‑148 (MQ=255)
                                                                         cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGG‑ATTTTGCGC‑TTTTTTCCCAGCCTTAAGACG‑aaa                                                                       >  2:2787829/1‑148 (MQ=255)
                                                                          tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCAATTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGG‑GTTTTTGGG‑TTTTTTGCCACCATTAAGAAG‑caaa                                                                      >  1:525706/1‑148 (MQ=255)
                                                                           gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAAt                                                                     <  1:888453/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                                  <  2:1590798/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAAGCATCGCGTAAAAACGCATTGGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                                  <  1:3302491/148‑1 (MQ=255)
                                                                              gTGGAGCAGGAACCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAACCGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGc                                                                  <  2:3359952/148‑1 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTGTGG‑TTTTTTGGCGTTTTTGCCC‑GCAATCATAAG‑AAAAATTCCCg                                                               >  1:2551031/1‑148 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCg                                                               <  2:2100364/148‑1 (MQ=255)
                                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTTTTGT‑TTTTTGGGG‑TTTTTGGCC‑GCAATGAGACGAAAAATTGccg                                                                >  1:784896/1‑147 (MQ=255)
                                                                                  aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTCAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCgg                                                              >  2:290510/1‑148 (MQ=255)
                                                                                  aGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTG‑TTTTTTTTTG‑GTTTTTGGC‑TTTTTTGCCAGCCATCAGACGAAAAATTGccgg                                                               >  1:229861/1‑146 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                            <  1:3437209/148‑1 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                            <  1:902279/148‑1 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGa                                                            <  1:2857262/148‑1 (MQ=255)
                                                                                    cAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTTTGGTTTTTTGGGT‑TTTTTGGCCGCCATAAGAACA‑AAAATTACCCggg                                                             >  1:700019/1‑148 (MQ=255)
                                                                                       gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTGTGG‑AGTTTTGGC‑TTTTTTGCCAGCCTTCAGACG‑AAAAATTCCCGGGGAtt                                                         >  1:281868/1‑148 (MQ=255)
                                                                                        aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtg                                                        <  2:580152/148‑1 (MQ=255)
                                                                                          gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATtgtg                                                      <  1:710141/148‑1 (MQ=255)
                                                                                          gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGG‑TTTTTGCGC‑TTTTTGCCCAGCATTAAGACG‑AAAATTGCCCCGGCAAtgtg                                                      >  2:2129111/1‑148 (MQ=255)
                                                                                              cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGaaaa                                                  <  2:1839502/148‑1 (MQ=255)
                                                                                              cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGaaaa                                                  <  2:34140/148‑1 (MQ=255)
                                                                                                cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TGTTGTTGGG‑CTTTTTGGC‑TTTTTTCCCCGCCTACAGACC‑AAAAAAGCCCCGGCATTTTTaaaaaa                                                >  2:1265366/1‑148 (MQ=255)
                                                                                                  tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGG‑TTTTTTCCCCGCATTCAGACG‑AAAAGTGCCCGGGAAGTGTGAAAAAATa                                              >  2:1322670/1‑148 (MQ=255)
                                                                                                   aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAc                                             <  2:1934426/148‑1 (MQ=255)
                                                                                                     aatcGCATTTGAAACTGTCGGCGGTTTTCCAGTATTTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATAcgc                                           <  1:3022702/145‑1 (MQ=255)
                                                                                                     aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTGTGG‑ATTTTTGGG‑TTTTTTCCCCGCATTGAGCCG‑AAAAATTCCCGGGCAGTGTGTAAAAATAagc                                           >  2:3673/1‑148 (MQ=255)
                                                                                                     aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGG‑ATTTTGCGC‑CTTTTGCCCAGCATTCAGACG‑AAAATTTCCCGGGAATTTTGAAAAAATAcgc                                           >  2:1120989/1‑148 (MQ=255)
                                                                                                              tGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                  <  1:242113/148‑1 (MQ=255)
                                                                                                                  aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTTTCTGG‑ATTTTTCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGTAAAAATAAGCGACCGCGCCCAAt                              >  2:1075677/1‑148 (MQ=255)
                                                                                                                  aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTATGG‑ATTTTGCGG‑TTTTTGCCCAGCATTACGACG‑AAAATTGCCCGGGAAGTGTGGAAAAAAAAGCGACACCGCCCaaa                              >  2:3444092/1‑147 (MQ=255)
                                                                                                                   cggTCGGCGATTTTCCTGTTTGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                             <  1:1032693/146‑1 (MQ=255)
                                                                                                                    tGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                            <  2:166566/148‑1 (MQ=255)
                                                                                                                       gggCGATTTCCCTGTATGTTCCAAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTGGCGC‑TTTTTGCCCAGCATTCAGACG‑AAATTTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCg                         <  1:2115218/147‑1 (MQ=255)
                                                                                                                       cGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTATGG‑ATTTTTCGC‑TTTTTTCCCACCAATCAGACG‑AAAAATGCCCGGGCATTTTTAAAAAATAACCGACAACGCGCAAAAACCg                         >  2:1840757/1‑148 (MQ=255)
                                                                                                                        ggCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                        <  2:2316959/148‑1 (MQ=255)
                                                                                                                         gcgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                       <  2:3537178/148‑1 (MQ=255)
                                                                                                                         gcgaTTTTCCAGTAGTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGtt                       <  1:2603371/148‑1 (MQ=255)
                                                                                                                          cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTTTGTGG‑ATTTTTCGC‑TTTTTTCCCAGCATTAAGACG‑AAAATTGCCCCGGAATTGTGAAAAAATAAGCGACAGCGCGCAATAAACGtta                      >  2:3477476/1‑147 (MQ=255)
                                                                                                                          cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCCGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCCtta                      >  2:2582301/1‑147 (MQ=255)
                                                                                                                             ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                   <  2:3155280/148‑1 (MQ=255)
                                                                                                                             ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTGTG‑TTTTGTGTGG‑ATTTTTCGG‑TTTTTTCCCAGCAATAAGACG‑AAAATTTCCCGGGAAATTTGAAAAAAAAAGCGACAACGCGCAAAAAACCTTCTCg                   >  1:2960312/1‑148 (MQ=255)
                                                                                                                                     tATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTTGGC‑TTTTTGCCCAGCAATGAGACG‑AAAAATTCCCGGGAAGTGTTTAAAAATAAACGACACCGCGCAAAAAACGATCTCGACACATaa           >  2:704296/1‑148 (MQ=255)
                                                                                                                                      aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTG‑TTTTGTCTTG‑ATTTTGCGC‑TTTTTTCCCAGCAATAAGACG‑AAAATTGCCCGGGCATTGTGAAAAAATAAGCGACAGCGCGCAAAAAACCTTCTCGACTCATaaa          >  2:2482560/1‑148 (MQ=255)
                                                                                                                                       tGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTTCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa         >  2:2051748/1‑148 (MQ=255)
                                                                                                                                       tGTTGCAAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaaa         <  1:112686/148‑1 (MQ=255)
                                                                                                                                         ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt       <  2:2707973/148‑1 (MQ=255)
                                                                                                                                         ttGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt       <  1:3085478/148‑1 (MQ=255)
                                                                                                                                          tGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTT‑TTTTTTCTGG‑GTTTTTGGC‑TTTTTTGCCACCATACAGACG‑AAAAATTCCCGGGCATTGTTTAAAAAAAATCCACACCGCGCCAAAAACCTTCGCGACTCATAAAATTg      >  2:921951/1‑148 (MQ=255)
                                                                                                                                            cTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt    <  1:3401906/148‑1 (MQ=255)
                                                                                                                                              aaaaATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc  >  2:1493400/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTG‑TTTTGTCTGG‑ATTTTGCGC‑TTTTTGCCCAGCATTCAGACG‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450145‑450434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCT  >  CP009273/450141‑450438
                                                                                                                                                     |                                                                                                                                                     
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAA                                                                                                                                                       >  SRR19973066.1466403/1‑150 (MQ=60)
 TTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAA                                                                                                                                                      >  SRR19973066.2445831/1‑150 (MQ=60)
   CATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATT                                                                                                                                                    >  SRR19973066.453689/1‑150 (MQ=60)
    ATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTT                                                                                                                                                   >  SRR19973066.2673042/1‑150 (MQ=60)
       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                                <  SRR19973066.1041869/150‑1 (MQ=60)
       CGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAT                                                                                                                                                <  SRR19973066.2703456/150‑1 (MQ=60)
            GNTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAA                                                                                                                                           >  SRR19973066.2726143/1‑150 (MQ=60)
                  CATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCC                                                                                                                                     >  SRR19973066.74444/1‑150 (MQ=60)
                  CATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAAAGGCC                                                                                                                                     >  SRR19973066.73228/1‑150 (MQ=60)
                     ACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGC                                                                                                                                  >  SRR19973066.2843211/1‑150 (MQ=60)
                          CAATATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGAATTTGAAACTGTCGGCGATTTTCAAGTATGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCC                                                                                                                             <  SRR19973066.1229833/150‑1 (MQ=60)
                           ACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCG                                                                                                                            <  SRR19973066.1118704/150‑1 (MQ=60)
                            CTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT                                                                                                                           >  SRR19973066.2387478/1‑150 (MQ=60)
                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑T                                                                                                                         >  SRR19973066.3560692/1‑150 (MQ=60)
                             TATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑G                                                                                                                         >  SRR19973066.3560729/1‑150 (MQ=60)
                               TTAAGGAGTGGGACGGGTGGCAGGCCACCGTCTTGGCCCCCCCGCCCTGTCGTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTT                                                                                                                       <  SRR19973066.1921456/150‑1 (MQ=60)
                                  AGGAGTGGGACGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTT                                                                                                                    <  SRR19973066.1629980/150‑1 (MQ=60)
                                  AGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTT                                                                                                                    <  SRR19973066.164253/150‑1 (MQ=60)
                                   GGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTT                                                                                                                   <  SRR19973066.3563937/150‑1 (MQ=60)
                                       TGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTttg                                                                                                               >  SRR19973066.1461280/1‑147 (MQ=60)
                                        GGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttgtgt                                                                                                               >  SRR19973066.3464384/1‑137 (MQ=60)
                                         GGATGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTG                                                                                                             <  SRR19973066.157597/150‑1 (MQ=60)
                                           AAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TGTGTTTTGTgtggg                                                                                                           >  SRR19973066.27335/1‑145 (MQ=60)
                                              GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgtggtgtt                                                                                                         >  SRR19973066.1675003/1‑131 (MQ=60)
                                              GGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtgttttttgtgggttt                                                                                                         >  SRR19973066.1538867/1‑131 (MQ=60)
                                               GTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTT                                                                                                       <  SRR19973066.3376679/150‑1 (MQ=60)
                                               GTTGCAGCACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCAGCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTT                                                                                                       <  SRR19973066.3376477/150‑1 (MQ=60)
                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttttgttgattttttggt                                                                                                   >  SRR19973066.476638/1‑126 (MQ=60)
                                                    AGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtttttgtgggtttttggggt                                                                                                   >  SRR19973066.117660/1‑126 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCCGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTT                                                                                                 <  SRR19973066.690174/150‑1 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgttttttttgggtttttggggt                                                                                                  >  SRR19973066.1298889/1‑124 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgttttttgttgtttttgggctt                                                                                                  >  SRR19973066.2261475/1‑124 (MQ=60)
                                                     GGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCTGGCTTTTGgggtt                                                                                                 >  SRR19973066.2938501/1‑145 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                              <  SRR19973066.1758467/150‑1 (MQ=60)
                                                        CACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTT                                                                                              <  SRR19973066.1727312/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTttgtggtttttggggtttttt                                                                                             >  SRR19973066.1751408/1‑129 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                             <  SRR19973066.2353861/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                             <  SRR19973066.801992/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTG                                                                                             <  SRR19973066.2353176/150‑1 (MQ=60)
                                                         ACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTttgttgttttttggggttttt                                                                                             >  SRR19973066.1751733/1‑129 (MQ=60)
                                                             TCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCA                                                                                         <  SRR19973066.2380299/150‑1 (MQ=60)
                                                              CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAG                                                                                        >  SRR19973066.2991446/1‑150 (MQ=60)
                                                              CTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTAAGTTGCTAAAAAATTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttttttttgtgggttttttgcac                                                                                         >  SRR19973066.3299657/1‑116 (MQ=60)
                                                                 TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                     <  SRR19973066.2991504/150‑1 (MQ=60)
                                                                 TGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAT                                                                                     <  SRR19973066.2991523/150‑1 (MQ=60)
                                                                  GCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATT                                                                                    <  SRR19973066.1783504/150‑1 (MQ=60)
                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCA                                                                                  <  SRR19973066.1549870/150‑1 (MQ=60)
                                                                    CTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGG‑TTTGTTTTttgttggttttgggcttttttgccccccataa                                                                                  >  SRR19973066.3509306/1‑118 (MQ=60)
                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGTgtttttttttgttgtgtttttggtttttttgcaccaaaaaaaa                                                                                >  SRR19973066.3355836/1‑107 (MQ=60)
                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggtttttttttttgggtttttgggttttttcgccgccataacac                                                                                >  SRR19973066.378616/1‑106 (MQ=60)
                                                                       TCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtttttttttggtttttggcttttttgccagcaaacagaa                                                                                >  SRR19973066.378614/1‑107 (MQ=60)
                                                                        CCCCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAAGTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAGGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACG                                                                              <  SRR19973066.936390/150‑1 (MQ=60)
                                                                         CTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAAAGGCCAGCGGGCCGT‑Tgttgtttttttttttttttatctattttgaaacccataaaaaaa                                                                             >  SRR19973066.3340939/1‑106 (MQ=60)
                                                                          TCCCTGTCGTGTATCATGAAGCATAGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTACCAGTATGTTGCTAAAATTTTTTTGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAA                                                                            <  SRR19973066.1414910/150‑1 (MQ=60)
                                                                           CCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAA                                                                           <  SRR19973066.3074975/150‑1 (MQ=60)
                                                                            CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTtggtgggtttttggctttttggccccccatcccaccaaaa                                                                          >  SRR19973066.529586/1‑110 (MQ=60)
                                                                            CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGGTTTTTGTGGGTTTTGGGGTTTTTTGCCAGCAGTCAGACGAAAA                                                                          >  SRR19973066.2333429/1‑150 (MQ=60)
                                                                            CCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCAATTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTttgtgggtttttgggttttttgccaccattaagaagcaaa                                                                          >  SRR19973066.529557/1‑110 (MQ=60)
                                                                               GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                       <  SRR19973066.894757/150‑1 (MQ=60)
                                                                               GTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTG                                                                       <  SRR19973066.2216489/150‑1 (MQ=60)
                                                                                 CGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgggggtttttgttggtttttggggtttttgccacccgtgagacccaacattcc                                                                      >  SRR19973066.367305/1‑97 (MQ=60)
                                                                                  GTGGAGCAGGAAGCATCGCGTAAAAACGCATTGGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAACACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCC                                                                    <  SRR19973066.3324333/150‑1 (MQ=60)
                                                                                  GTGGACCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCC                                                                    <  SRR19973066.934682/150‑1 (MQ=60)
                                                                                   TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTttgtggttttttggcgtttttgcccgcaatcataagaaaaattcccg                                                                   >  SRR19973066.2568358/1‑103 (MQ=60)
                                                                                   TGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTtttttgttttttggggtttttggccgcaatgagacgaaaaattgccg                                                                   >  SRR19973066.790688/1‑103 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGgggtggtttgtgtgggtttttgggtttttggcccgcagtcagacgcaaatttgccgg                                                                   >  SRR19973066.706853/1‑93 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtttttttttggtttttggcttttttgccagccatcagacgaaaaattgccgg                                                                   >  SRR19973066.231692/1‑94 (MQ=60)
                                                                                    GGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TGTGTTTTGTtttggtttttggggtttttggcccccaagagcaccaaattggccgg                                                                  >  SRR19973066.706609/1‑104 (MQ=60)
                                                                                      AGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTttttggttttttgggttttttggccgccataagaacaaaaattacccggg                                                                >  SRR19973066.705181/1‑100 (MQ=60)
                                                                                        CAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                              <  SRR19973066.2876455/150‑1 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                              <  SRR19973066.908637/150‑1 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                              <  SRR19973066.3460205/150‑1 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                              <  SRR19973066.3080387/150‑1 (MQ=60)
                                                                                        CAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAAT                                                              <  SRR19973066.3460344/150‑1 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTtttttttgtggttttttgggttttttgccagcattgagacgaaaaaagaccgggcaat                                                             >  SRR19973066.143406/1‑92 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTGTGGAGTTTTGGCTTTTTTGCCAGCCTTCAGACGAAAAATTCCCGGGGATT                                                             >  SRR19973066.283918/1‑150 (MQ=60)
                                                                                         AGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTtttggtttttgggtttttttccacccataagaccaacaaatgccgggccgt                                                             >  SRR19973066.3151865/1‑99 (MQ=60)
                                                                                              GCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAA                                                        <  SRR19973066.3483319/150‑1 (MQ=60)
                                                                                              GCATCGCGTAAAAACGCATTTGAAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAA                                                        <  SRR19973066.715402/150‑1 (MQ=60)
                                                                                                ATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgggtgtttttgttgttttttggggtttttggccgcagtaaaaacaaaaaatccccgggagttggaaaa                                                       >  SRR19973066.343143/1‑82 (MQ=60)
                                                                                                  CGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCCGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttttggtttttttggtgtttttggcgccattaagcgacaacatgccggggcggtggtgaata                                                     >  SRR19973066.25729/1‑80 (MQ=60)
                                                                                                         AATCGCATTTGAAACTGTCGGCGGTTTTCCAGTATTTTGCTAAAAATTTTATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGA                                             <  SRR19973066.3042866/150‑1 (MQ=60)
                                                                                                         AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggttgttttttgtgggtttttggggtttttgcccgcaatcagaagaaaaatggccggggcttgtgtaaaaataacagc                                              >  SRR19973066.2626389/1‑72 (MQ=60)
                                                                                                         AAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGggggggttttttttggtttttgtggtttttggccaccagtagacgtaaaaaggacgcggggtttgtaaaaaaaaacca                                              >  SRR19973066.2135692/1‑72 (MQ=60)
                                                                                                          AACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCAGCGGGCCGT‑GTTGTTTTTTGTGGATTTTGtggttttttggcagcaataaaaacaaaattgggccgggattttgtaaaaataaaagca                                            >  SRR19973066.2593632/1‑92 (MQ=60)
                                                                                                           ACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACA                                           <  SRR19973066.906177/150‑1 (MQ=60)
                                                                                                           ACGCATTTGAAACTGTCGGCGCTTTTCCAGTTTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACA                                           <  SRR19973066.1726426/150‑1 (MQ=60)
                                                                                                            CGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgtttttttttgttgtttgggtttttttccccccaaaagaacaaaaattgcccgggcttttgtaaaaaaaccccccac                                           >  SRR19973066.2743060/1‑70 (MQ=60)
                                                                                                                TTTGCAAGTGTGGGCGGTTTTCCATTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGACTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCG                                      <  SRR19973066.3068872/150‑1 (MQ=60)
                                                                                                                  TGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCA                                    <  SRR19973066.244005/150‑1 (MQ=60)
                                                                                                                  TGAAACTGTCGGCGATTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCA                                    <  SRR19973066.238840/150‑1 (MQ=60)
                                                                                                                      ACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATTAAAAAAGGCCTGCCGGCCGTgttttttttttttggttttttgggttttttgcacccaaaaaaacaaaaatagccccgcaattttttaaaaaaaaagacaccccccccaaa                                 >  SRR19973066.3081236/1‑60 (MQ=52)
                                                                                                                       CTGTCGGCGCTTTTCCAGTTTGTTGATAAAATTTTTATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAC                               <  SRR19973066.1040375/150‑1 (MQ=60)
                                                                                                                       CGGTCGGCGATTTTCCTGTTTGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAC                               <  SRR19973066.1040293/150‑1 (MQ=60)
                                                                                                                        TGTCGGCGCTTTTCCATTATGTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACC                              <  SRR19973066.1159867/150‑1 (MQ=60)
                                                                                                                           ggggGCTTTTCCATTATGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTT                           <  SRR19973066.2127763/146‑1 (MQ=60)
                                                                                                                             GCGCTTTTCCAGTATGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                         <  SRR19973066.2298717/150‑1 (MQ=60)
                                                                                                                             GCGATTTTCCAGTAGTTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCT                         <  SRR19973066.2620978/150‑1 (MQ=60)
                                                                                                                              CGCTTTTCCATTATGTTGCAAAAAATTTTATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTC                        <  SRR19973066.702771/150‑1 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtggtttttgttgttttttgggtttttttgcccccatcagacgaaaaaatgcccgggattttgtaaaaaaaaaagcccacccgccaaaaacccggtgg                        >  SRR19973066.73196/1‑51 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TGTGTTTTGTGTGGATTTTTCGGTTTTTTCCCAGCAATAAGACGAAAATTTCCCGGGAAATTTGAAAAAAAAAGCGACAACGCGCAAAAAACCTTCTCG                       >  SRR19973066.2979919/1‑150 (MQ=60)
                                                                                                                               GCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCG                       <  SRR19973066.776168/150‑1 (MQ=60)
                                                                                                                                 TTTTCCATTATTTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAC                     <  SRR19973066.2523474/150‑1 (MQ=60)
                                                                                                                                 TTTCCCAGTATGTTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAC                     <  SRR19973066.2521922/150‑1 (MQ=60)
                                                                                                                                  TTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggttttttttgatgttttttggcttttttcccagaaaacaaaacaaaaattcccggggatttttaaaaaataaacgaccgcgcgccaaaacccagctcgact                     >  SRR19973066.2831193/1‑48 (MQ=60)
                                                                                                                                    TCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttattgaatttatttttggctatttgtccaacagtcagaaaaacattggaaggtaagtttttaaaaaatatccaaagcgccccaaaacagatatcttatca                   >  SRR19973066.2021462/1‑46 (MQ=60)
                                                                                                                                    TCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTttgttgttttttggcttttttgccagcaataagacgaaaaatgcccggggaatgtgtaaaaaaaagcgacaccgcccccaaaaccttgtcgacaca                  >  SRR19973066.2021611/1‑54 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTggtgtttttttgggatttagaattttattcctactaaacaacaaaaattaccggggattgtgagaaaaaaacccaaaccaccgcaaaaaaatactacacagaaaa               >  SRR19973066.1335086/1‑45 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTttttttttttgtttttttggttttttgccccccataagaagaaaaaataccgggcattggttaaaaaaaaacgacaacgcgcccaaacccgtctcgacacaaaa               >  SRR19973066.1851179/1‑46 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTttttgtttttttggcgtttttgccacccataagaagaaaaattccccgggaattttaaaaaaaaaaaaccacccccccccaaaccttgaggacacaaaa               >  SRR19973066.1851232/1‑51 (MQ=60)
                                                                                                                                       AGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTttgtggctttttggcgtttttgcccgccataagacgaaaaaatccccggcaatttgaaaaaataacccaaaacccgcccaaaaccaaaacgacaaaaaa               >  SRR19973066.3428053/1‑51 (MQ=60)
                                                                                                                                        GTATGTTGCTGAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttgtatttttttttcgcgtttttaacagccaaaagaacaaaaattccccgggcttgtgtaaaaaaaaccgacagcgcgcaaaaaacactcacgaataaaaaa               >  SRR19973066.2174099/1‑42 (MQ=60)
                                                                                                                                        GTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttgtttttttgcttttttgccaccattaatacgaaaaaatgcccggtattttttaaaaaaaaacgacaccgcgccaaaaaccatatagacaaaaaaa               >  SRR19973066.1851756/1‑42 (MQ=60)
                                                                                                                                        GTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgttttttttttttggttttttgggtttttgccaccaaacaaaacaaaaaatgaccgggattttgtaaaaaaaatagaaaaccccccaaaaaactaaaacacaaaaaaa               >  SRR19973066.1851771/1‑42 (MQ=60)
                                                                                                                                        GTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTggtgttttttttggttttttggctttttggcacccaaaaagacgaaaaattgcccggacatttttaaaaaaaaaccacaaccccccaaaaaccgactcgacaaaaaaa               >  SRR19973066.1851668/1‑42 (MQ=60)
                                                                                                                                        GTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAA              <  SRR19973066.3440615/150‑1 (MQ=60)
                                                                                                                                         TATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTttttttttggggttttttgcttttttgccccccaagagtcgaaaatatggcggggattttgtaaaaataaaagacagcgccccaaaaaacgtctgggcgaaaaaaa             >  SRR19973066.1285558/1‑44 (MQ=60)
                                                                                                                                          ATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTGTTTTttttgatttttgggttttttgccagcaatgagaagaaaattggcccgggattttgtaaaaataaacgacagcgcgccaaaaaacttatagactcaaaaaaa             >  SRR19973066.515031/1‑49 (MQ=60)
                                                                                                                                           TGTTGCAAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGT           <  SRR19973066.113836/150‑1 (MQ=60)
                                                                                                                                             TTGATAAAAATTTTATCAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGA         <  SRR19973066.3106095/150‑1 (MQ=60)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTgtttttttgtgtggtgttttggcttttttgccagccttgagtagcaaaattcccggggattttgtaaaaataagccacagagcgcgaaaaacggtgtggactcaaaaaattgat         >  SRR19973066.537400/1‑36 (MQ=60)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTGTGGTTTTTTCGCGTTTTGGCCAGCATTAAGACGAAAAATTCCCGGGGATTGTTAAAAAAaaaaccgaagcgcgccataacacgtatagactgaaaaaagtgat        >  SRR19973066.537694/1‑106 (MQ=51)
                                                                                                                                              TGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAT        <  SRR19973066.3181525/150‑1 (MQ=60)
                                                                                                                                                CTAAAAATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC      <  SRR19973066.3424618/150‑1 (MQ=60)
                                                                                                                                                    AAATTTTATGAAAAACGGCCGGCGGGCCGT‑TTTGTTTTGTCTGTATTTTGCGCTTTTGGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCT  <  SRR19973066.348285/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTTCATGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCT  >  CP009273/450141‑450438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑