Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,463,906 C→A 27.6% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,463,9060CA27.6% 309.6 / 54.1 172Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (29/90);  new base A (44/8);  total (74/98)
Fisher's exact test for biased strand distribution p-value = 1.21e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

TGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAT  >  CP009273/2463758‑2464052
                                                                                                                                                     |                                                                                                                                                  
tGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTc                                                                                                                                                    <  1:2755885/149‑1 (MQ=255)
 gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCa                                                                                                                                                   >  2:4129391/1‑149 (MQ=255)
   agtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCaaa                                                                                                                                                 <  1:1550938/149‑1 (MQ=255)
      aTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAaaaaaa                                                                                                                                              >  2:4633265/1‑149 (MQ=255)
       tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTTATTTATAAAATAAATaaaaaaaa                                                                                                                                             >  1:4355580/1‑149 (MQ=255)
       tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTTATTTATAAAATAAATaaaaaaaa                                                                                                                                             >  1:2957196/1‑149 (MQ=255)
       tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTAtaaaaaaaataaaaaaaa                                                                                                                                             >  1:4355617/1‑149 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                                                                                           <  2:1111560/149‑1 (MQ=255)
          ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATaaaataaataaaataaataa                                                                                                                                          >  2:3055591/1‑147 (MQ=255)
            ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                        <  2:4634764/149‑1 (MQ=255)
                ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                                                                                                     <  1:3702516/149‑1 (MQ=255)
                   atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                 <  2:2821644/149‑1 (MQ=255)
                    tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAAATCAAATAAAAAAATACAATAGc                                                                                                                                >  2:648421/1‑149 (MQ=255)
                     atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAAATTTTAAAATAATAAAAAGAAATAAAGAAAATAgag                                                                                                                               >  2:4453307/1‑147 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                                                                                            <  2:1941709/149‑1 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                                                                                            <  1:315006/149‑1 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                                                                                            <  2:4026155/149‑1 (MQ=255)
                          aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTTATTTATAAAATAATTAAAATAAATAAAGAAAAAATCGATGtt                                                                                                                          >  1:1059966/1‑148 (MQ=255)
                            ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TGTTTTTTATTTATAAAAAAAATAAAAGAAAAAAAAACAAAATCGAAGTaaa                                                                                                                        >  1:2226369/1‑149 (MQ=255)
                            ttttATGTGTCATTTTTCGGGGAAGGACATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                        <  1:1918838/149‑1 (MQ=255)
                                aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                                                                                                    <  2:4313540/149‑1 (MQ=255)
                                aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAAAAAATGAAAATAAAAAAAACAAAATCGATTTAAAgaaa                                                                                                                    >  2:1276745/1‑146 (MQ=255)
                                 tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                                                                                                   <  2:3227361/149‑1 (MQ=255)
                                  gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                                                                                                  <  1:1241132/149‑1 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                               <  1:316412/149‑1 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                               <  2:4776371/149‑1 (MQ=255)
                                      cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                              <  1:1304658/149‑1 (MQ=255)
                                         ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAATCAAAAAAAAATCGATTTAAAGCAAAAATTTTaa                                                                                                           >  2:1977882/1‑149 (MQ=255)
                                              ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TGTTTTTTATTTTTAAAAAAAATAAAAGAAATAAAAAAAATATCGAAGTTAAGCACGAAGtttttattta                                                                                                      >  1:3411162/1‑142 (MQ=255)
                                               gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGCGATGTAAAACACTAAGTTTAAAtttag                                                                                                     >  2:3471735/1‑146 (MQ=255)
                                                ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAAAAATTAAAAGTAAAAAATACAATAAAGATGTAAAGCACTAATTTTAAAttttgc                                                                                                    >  2:4582320/1‑145 (MQ=255)
                                                ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATATAAAAAATAAATAAAAAAAATAAAGACAAAATAGATGTTAATCACTAACTTTTAAtttaga                                                                                                    >  2:181914/1‑145 (MQ=255)
                                                ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAAATTATAAAATAATTAAAAGAAATCAAGACAAAAACGATGGAAAGCACAAATTTTAAATTgcgc                                                                                                    >  2:181238/1‑149 (MQ=255)
                                                   aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTTATTTATAAAAAAAATAAAAGAAAACAAAAAAAAAACGATTTAAAGCACGAAGTTTTAATTTAGCGcc                                                                                                 >  1:4724858/1‑149 (MQ=255)
                                                        ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                            <  2:1770014/149‑1 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                         <  2:2475253/149‑1 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                         <  2:2409905/149‑1 (MQ=255)
                                                           gagaTTTAAGCAAGAATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                         <  2:2411959/149‑1 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                      <  2:2320884/149‑1 (MQ=255)
                                                                ttAAGAAATTATTTATTTTTATTTTTTTTTTCGTTCTAAATTTTATTATACATAACAGTGT‑TTTTTTTAATTTATAAAATAATTAAAATAAATCAAGAAATTAGCGTTTTAAATCACTATTTTTAATTTGCGCGCCAATCATGgcgcgc                                                                                    <  1:4154231/149‑1 (MQ=255)
                                                                 tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAAAAAATAAAAAAAAACAATAAAAAATCCATTTAAAAAACTAACTTTTAAATTCGCGCCCAAAATGgcgcccc                                                                                   >  2:1757456/1‑146 (MQ=255)
                                                                     caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAAATTATAAAAAAAATAAAAAAAAAAAAGAAAAAATCGATTTAAATAACAAATTTTAAATTTCGCGCCAAACAATGCGCGCCCaac                                                                               >  2:521410/1‑148 (MQ=255)
                                                                      aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTTTAAAATAATTAAAAGAAAAAAAGAAAATATCGATGTAAATCACTAAGTTTAAATTTAGCGCCAATCATTGCGCGCACAAGc                                                                              >  2:2328889/1‑149 (MQ=255)
                                                                       agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                             <  2:2280302/149‑1 (MQ=255)
                                                                        gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                            <  2:3146841/149‑1 (MQ=255)
                                                                        gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAAATAAAATTAATAAATACAAAAGCGATTTAAAGCACGAATTTTTTATTGCGAGCCAATAATTTCGCGCACAAgaga                                                                            >  2:3573069/1‑146 (MQ=255)
                                                                          atttttttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtata                                                                          <  2:4357699/149‑1 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAATAAATCAACACAAAAGCGATGTAAAGCACTAAGTTTATATTGCGCGCCAAACATGGCGCGCACAAGCgata                                                                          >  2:1098561/1‑149 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtata                                                                          <  2:734376/149‑1 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGAGCGCCAATCATGGCGCGCACAACCtata                                                                          >  2:2330286/1‑149 (MQ=255)
                                                                              atttTGAATTTATTTTTCGTTCTACGTTTGATTATAAATAACAGTCT‑TTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAc                                                                      <  1:2635221/149‑1 (MQ=255)
                                                                              atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAAAATCGATGTAAAGCACAAATTTTAAAATTCGCGCCCAACAATGCGCGCACAAGCAaaaataa                                                                      >  2:4721083/1‑148 (MQ=255)
                                                                               tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                     <  2:3244638/149‑1 (MQ=255)
                                                                               tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TGTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAAACAAAAACGAATTAAAAAACAAAGTTTTAATTTAGCGCCAAACAGTGGGCGCCCAACCAaaaataac                                                                     >  1:1559799/1‑147 (MQ=255)
                                                                                  ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAAAAAATAAAAGAAATAAATAAAATATCGATGTAAAGCACAAAGTTTAAATTTAGCGCCAAAAAATGCGCGCACAATCGATAAAACCAAc                                                                  >  2:3514398/1‑149 (MQ=255)
                                                                                  ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAAAAAATAAAAGAAAAAAAAACAAAATCGATTTAAAACACAAATTTTAAAATTGGCGCCAATAAATGCGCGCCCAACCTataaaaaacac                                                                  >  2:237121/1‑142 (MQ=255)
                                                                                   gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcc                                                                 <  2:1813477/149‑1 (MQ=255)
                                                                                      tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTaa                                                              <  2:4552311/149‑1 (MQ=255)
                                                                                       gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                             <  2:3816174/149‑1 (MQ=255)
                                                                                       gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTAG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                             <  2:3816103/149‑1 (MQ=255)
                                                                                        tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGAATTAAATCACTAAGTTTAAATTTCGCGCCCATCAAGGCGCGCCCAAACAAAAAAACCAACCaaaat                                                            >  2:728692/1‑147 (MQ=255)
                                                                                          tttttCGGTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                          <  2:3627139/149‑1 (MQ=255)
                                                                                            tttCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAAATTAAAAAATAAATAAAAAAAAAAAAAAAAAAAAAGCTGTAAAACACAAAGTTTAAAATTAGAGCCAACAATGGCGGGCCCAACCTATAAAAACAACCaaaaataac                                                        >  2:6011/1‑143 (MQ=255)
                                                                                                  tcgACCTTTGATTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                  <  1:3064290/149‑1 (MQ=255)
                                                                                                   cgACCTTTGATTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCctct                                                 <  1:1050808/149‑1 (MQ=255)
                                                                                                          tGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                          <  1:3719666/149‑1 (MQ=255)
                                                                                                              tataCATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                      <  1:2889625/149‑1 (MQ=255)
                                                                                                              tataCATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                      <  1:2700781/149‑1 (MQ=255)
                                                                                                                 aaaTAACAGTCT‑TTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                   <  1:3018226/147‑1 (MQ=255)
                                                                                                                 aCATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                   <  2:2278177/149‑1 (MQ=255)
                                                                                                                  cATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtc                                  <  2:4255425/149‑1 (MQ=255)
                                                                                                                         gTCG‑TTTTTTTAATTTATAAAATAATTAAAATAAAACAAGAAAAAATCGATGTAAATCACTAAGTTTATATTTCGCGCCAATCATGGCGCGCACAAGCTAAAATAACAACCTAATTTCTACTACTCTTAGAGTGACTATATATCCTGAt                           >  2:3002043/1‑149 (MQ=255)
                                                                                                                          tCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAAAAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCAAAATTCTCCTCCACTTAGAGTGACTATATCTCCTGATa                          >  1:703982/1‑149 (MQ=255)
                                                                                                                              tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                       >  2:3835433/1‑149 (MQ=255)
                                                                                                                              tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                       >  1:2834485/1‑149 (MQ=255)
                                                                                                                               ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                      <  1:1048289/149‑1 (MQ=255)
                                                                                                                               ttttttAAATTATAAAATAATTCAAATAAATCAAGACAATAGCTTTTTAAAGAACTAAGTTTAAATGGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                      <  1:4448755/149‑1 (MQ=255)
                                                                                                                                tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                     >  2:2528510/1‑149 (MQ=255)
                                                                                                                                 ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                    <  1:3318967/149‑1 (MQ=255)
                                                                                                                                  tttAATTTATAAAATAATTCAAATAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                   <  1:911349/149‑1 (MQ=255)
                                                                                                                                  tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                   <  2:4484041/149‑1 (MQ=255)
                                                                                                                                    tAATTTATAAAATAATTCAAAGAAATCAAGACAATCGCGATTTAAAGCACTAAGTTTAAATGGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                 <  1:2106447/149‑1 (MQ=255)
                                                                                                                                     aaTTTAAAAAATAATACAAATAAATCAAGAAAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                <  1:3808060/149‑1 (MQ=255)
                                                                                                                                       tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt              <  2:946441/149‑1 (MQ=255)
                                                                                                                                       tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt              >  1:167007/1‑149 (MQ=255)
                                                                                                                                       tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt              <  1:4521981/149‑1 (MQ=255)
                                                                                                                                        ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt             >  1:3617305/1‑149 (MQ=255)
                                                                                                                                            aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt         <  1:2117587/149‑1 (MQ=255)
                                                                                                                                            aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCGCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt         <  1:3471735/149‑1 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt       >  2:117731/1‑149 (MQ=255)
                                                                                                                                               ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc      <  2:1956101/149‑1 (MQ=255)
                                                                                                                                                taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa     >  2:2747477/1‑149 (MQ=255)
                                                                                                                                                 aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa    >  2:531748/1‑149 (MQ=255)
                                                                                                                                                 aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa    >  2:873426/1‑149 (MQ=255)
                                                                                                                                                  atacAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa   <  1:4045174/146‑1 (MQ=255)
                                                                                                                                                  atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa   >  2:2868012/1‑149 (MQ=255)
                                                                                                                                                  atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa   >  2:2599912/1‑149 (MQ=255)
                                                                                                                                                  atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa   <  1:2062409/149‑1 (MQ=255)
                                                                                                                                                  atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa   <  1:1980623/149‑1 (MQ=255)
                                                                                                                                                   ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt  >  2:824242/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAT  >  CP009273/2463758‑2464052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A