Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F60 I0 R1 465 320.4 10502068 95.5% 10029474 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,463,906 C→A 14.2% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,463,9060CA14.2% 384.6 / 14.0 137Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (31/80);  new base A (23/2);  total (55/82)
Fisher's exact test for biased strand distribution p-value = 2.78e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

TAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGC  >  CP009273/2463760‑2464054
                                                                                                                                                  |                                                                                                                                                      
   taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGa                                                                                                                                                   <  1:4951861/149‑1 (MQ=255)
    aTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGaa                                                                                                                                                  >  2:880741/1‑149 (MQ=255)
     tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATTAAAAAAataaaataaa                                                                                                                                                 >  1:5176525/1‑149 (MQ=255)
     tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATTAAATAATTAAAATaaa                                                                                                                                                 >  1:3058965/1‑149 (MQ=255)
      ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTAtaaaaaaaataaaaaaaaa                                                                                                                                                >  1:143951/1‑148 (MQ=255)
       tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTTTTTATAAAATAATTAAAATaaaag                                                                                                                                               >  2:1326392/1‑147 (MQ=255)
       tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTTTTTATAAAAAAATTATAATAAAta                                                                                                                                               >  2:1326495/1‑148 (MQ=255)
       tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAaaataaata                                                                                                                                               >  2:2949018/1‑148 (MQ=255)
              ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTCTAAAATAATTCAAAGAAATCAAGACaa                                                                                                                                        <  1:2155856/149‑1 (MQ=255)
              ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                                                                                                        <  1:4615691/149‑1 (MQ=255)
               gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTTTTAAATAAATAAAATAAATAAAAAAAAt                                                                                                                                       >  1:1020497/1‑149 (MQ=255)
                    tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                  <  1:4056225/149‑1 (MQ=255)
                    tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                  <  1:4151107/149‑1 (MQ=255)
                    tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                  <  2:1336731/149‑1 (MQ=255)
                     ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                 <  1:701787/149‑1 (MQ=255)
                     ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                 <  1:486291/149‑1 (MQ=255)
                      tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                                                                                                <  1:3401966/149‑1 (MQ=255)
                         aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCGAGACAATAGCGATGTaa                                                                                                                             <  2:1080454/149‑1 (MQ=255)
                            ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                          <  2:3765403/149‑1 (MQ=255)
                            ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                          <  2:1587317/149‑1 (MQ=255)
                             tATGTGTCATTTTTCGGGGAAGGATATAGAGAATTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                         <  1:3910142/149‑1 (MQ=255)
                                 tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAAAAAAAAATAAAAAATCCATGTAAAGCACTaa                                                                                                                     >  2:630666/1‑149 (MQ=255)
                                 tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                     <  2:3086183/149‑1 (MQ=255)
                                  gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAATACAAAAGCGATTTAAATCACaaaa                                                                                                                    >  2:1288600/1‑148 (MQ=255)
                                         ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAAt                                                                                                            <  2:3974055/149‑1 (MQ=255)
                                            ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAAAAAAAAAAAAAAAAAACGATGTTAATAACAAAA‑TTTAAAttta                                                                                                         >  2:3167704/1‑147 (MQ=255)
                                            ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑gc                                                                                                        <  2:961832/149‑1 (MQ=255)
                                            ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑gc                                                                                                        <  2:3942446/149‑1 (MQ=255)
                                             gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACGAAG‑TTTAAATT‑tcg                                                                                                       >  2:5148513/1‑149 (MQ=255)
                                              tgAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATTTATTTTTCGTTCGACATTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑gcgc                                                                                                      <  1:1976112/148‑1 (MQ=255)
                                              ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAAAAAAAAAACAAAAAATCGATGTTAAGGCCAAAG‑GTTAAAtttagc                                                                                                       >  1:5129924/1‑145 (MQ=255)
                                                  ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTTAAATAATTCAAATAAAAAAAGACCAAATCGATGTGAATCACGAAG‑TTTTATTT‑TAGCGccc                                                                                                  >  2:3682232/1‑148 (MQ=255)
                                                  ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAAAAAACAAGAAAAAATCGATTTAAAGCACAAAT‑TTTAAATT‑GCGCGCCa                                                                                                  >  2:2588487/1‑149 (MQ=255)
                                                   gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCaa                                                                                                 <  1:4590268/149‑1 (MQ=255)
                                                    atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAAt                                                                                                <  1:2346623/149‑1 (MQ=255)
                                                      ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCa                                                                                              <  2:5097806/149‑1 (MQ=255)
                                                       taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCAt                                                                                             <  1:5201715/149‑1 (MQ=255)
                                                       taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCAt                                                                                             <  1:2023544/149‑1 (MQ=255)
                                                                     atattttttttGGATTTTTTTTTTCGTTCTACTTTTTCTTATAAATAACTTTTTTTTTTTTATTTTATAAATTAATTAAAATAAATAAATACAATCGCTATGTAAAGAACTAAT‑TTTAATTT‑GCGCGCAAATCATGGCGCGCACAAGCt                                                                               <  1:34759/145‑1 (MQ=255)
                                                                            atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATAc                                                                        <  1:3458936/149‑1 (MQ=255)
                                                                                ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAGACAATATCGATGTAAATCACTAAG‑TTTAAATT‑TCGCGCCCAACATGGCGCGCACAAGCGATAATACCaaa                                                                    >  2:3451866/1‑148 (MQ=255)
                                                                                   aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTa                                                                 <  2:861567/149‑1 (MQ=255)
                                                                                   aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAATAAAAAATCGATGTAAAGCACTAAG‑TTTAAATT‑TCGCGCCAAAAATGGCGCGCACAAGCAATAAAAACAAccaa                                                                 >  2:3437792/1‑147 (MQ=255)
                                                                                      tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAATAAAAAAAAAAAAAAAAAAAAAAAACGATGGTAAACAAAAAGTTTTAAATTAGCGC‑CCCACCAT‑GCGCGCCCAAGCAATAATAACaaaaacaaa                                                              >  1:2170122/1‑142 (MQ=255)
                                                                                       aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                             <  2:3325242/149‑1 (MQ=255)
                                                                                          tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                          <  2:1081100/149‑1 (MQ=255)
                                                                                              gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                      <  2:1630050/149‑1 (MQ=255)
                                                                                               ttcgACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                     <  1:5122304/149‑1 (MQ=255)
                                                                                                tcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                    <  2:3829259/149‑1 (MQ=255)
                                                                                                tcgACCTTTGATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                    <  1:861757/149‑1 (MQ=255)
                                                                                                tcgACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATTTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                    <  1:4219416/149‑1 (MQ=255)
                                                                                                 cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCctct                                                   <  2:35722/149‑1 (MQ=255)
                                                                                                  gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                  <  2:491805/149‑1 (MQ=255)
                                                                                                   aCCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATATCGATGTAAATCACTAAG‑TTTTAATT‑TAGCGCCAATCATGGCGCGCACAAGCTCTAAAAACAAACACAAATATCCTCCTCTTa                                                 >  2:4327172/1‑149 (MQ=255)
                                                                                                      tttGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                              <  2:4681704/149‑1 (MQ=255)
                                                                                                      tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                              <  2:2065991/149‑1 (MQ=255)
                                                                                                      tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                              <  2:4794168/149‑1 (MQ=255)
                                                                                                        tgattATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                            <  2:3839075/146‑1 (MQ=255)
                                                                                                             ataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                       <  2:1331892/149‑1 (MQ=255)
                                                                                                              taCATAACAGTCGTTTTTTTTATTTATAAAAAAATTGAAAAAAATAAAAAAAAAATCGATGTTAATCAATAAG‑TTTAAATT‑TGGCGCCAATCAAGGCGCGCCCAAGCAATAATAACAACAAAAATTATACTCCACTTATAGttaataaa                                      >  2:2540903/1‑142 (MQ=255)
                                                                                                              taCATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAGCGATGTAAAGCAATAAG‑TTTAAATT‑TCGCGCCAAACATTGCGCGCACCAGCTATAAAACCAACCAAAATTCTACAACTAGTAGAGTGAAtaaa                                      >  2:2540743/1‑147 (MQ=255)
                                                                                                                 aTAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtct                                   <  1:3660948/149‑1 (MQ=255)
                                                                                                                  tAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                  <  1:1457474/149‑1 (MQ=255)
                                                                                                                  tAACAGTCGTTTTTTTAATTTATAAAATATTTAAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAG‑TTTAATTT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                  <  1:4967161/149‑1 (MQ=255)
                                                                                                                  tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                  <  1:4222419/149‑1 (MQ=255)
                                                                                                                        tCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGAAAAAAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGGCAATCCTGGCGCGCACAAGCTATAATAACAACCAAATTTATCCTCCGCTTATAGTGACTAAATATACTGATa                            >  1:1202815/1‑149 (MQ=255)
                                                                                                                          gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                          >  2:1159792/1‑149 (MQ=255)
                                                                                                                           ttttttaAATTTATAAAATAATTCAAATAAATAAAGACAATAGCTATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                         <  1:4933341/149‑1 (MQ=255)
                                                                                                                            ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                        <  2:4624892/149‑1 (MQ=255)
                                                                                                                            ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                        <  2:4624779/149‑1 (MQ=255)
                                                                                                                                ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCTATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    <  1:2991177/149‑1 (MQ=255)
                                                                                                                                ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    <  1:2991193/149‑1 (MQ=255)
                                                                                                                                 tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                   <  2:1974531/149‑1 (MQ=255)
                                                                                                                                 tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                   <  1:5111137/149‑1 (MQ=255)
                                                                                                                                  aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                  <  1:4813703/149‑1 (MQ=255)
                                                                                                                                        taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc            >  1:559542/1‑149 (MQ=255)
                                                                                                                                        taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc            >  2:2310173/1‑149 (MQ=255)
                                                                                                                                        taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc            >  2:4826680/1‑149 (MQ=255)
                                                                                                                                          aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa          >  1:3785609/1‑149 (MQ=255)
                                                                                                                                          aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCCGGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa          >  1:2533384/1‑149 (MQ=255)
                                                                                                                                          aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa          <  2:115706/149‑1 (MQ=255)
                                                                                                                                          aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa          >  1:4394313/1‑149 (MQ=255)
                                                                                                                                          aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa          >  1:4394321/1‑149 (MQ=255)
                                                                                                                                           aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCTCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGTTAGAATTGCGGTATTGACTAt         >  1:1583928/1‑149 (MQ=255)
                                                                                                                                           aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt         <  2:2677240/149‑1 (MQ=255)
                                                                                                                                            ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc        <  2:3619654/149‑1 (MQ=255)
                                                                                                                                             taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa       >  2:226788/1‑149 (MQ=255)
                                                                                                                                              aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCACTCATTGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa      >  1:3109664/1‑149 (MQ=255)
                                                                                                                                              aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATTGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa      >  1:3109753/1‑149 (MQ=255)
                                                                                                                                              aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa      >  1:531276/1‑149 (MQ=255)
                                                                                                                                              aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa      >  2:4967570/1‑149 (MQ=255)
                                                                                                                                              aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa      <  1:3233208/149‑1 (MQ=255)
                                                                                                                                               atacAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa     <  1:2915673/146‑1 (MQ=255)
                                                                                                                                                ttCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAT‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt    <  1:3566651/149‑1 (MQ=255)
                                                                                                                                                ttCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt    <  1:5078723/149‑1 (MQ=255)
                                                                                                                                                 tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg   >  2:4687323/1‑149 (MQ=255)
                                                                                                                                                  cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc  <  2:143951/149‑1 (MQ=255)
                                                                                                                                                  cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc  <  2:144027/149‑1 (MQ=255)
                                                                                                                                                  cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc  >  1:2857814/1‑149 (MQ=255)
                                                                                                                                                  cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc  >  2:2898807/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAG‑TTTAAATT‑GCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGC  >  CP009273/2463760‑2464054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A