Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F17 I0 R1 66 109.4 2318666 93.6% 2170271 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 433,649 T→G 19.9% G65G (GGT→GGG)  ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913433,6490TG19.9% 132.7 / 8.5 105G65G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (27/50);  new base G (14/9);  total (46/59)
Fisher's exact test for biased strand distribution p-value = 3.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

Only 100 of 107 total aligned reads displayed.

AGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCACC  >  NC_000913/433415‑433886
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
aGAATTGGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑AAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGa                                                                                                                                                                                                                                           <  1:461136/239‑1 (MQ=255)
   aTTTGGCGAAGAGTGCCCGCGCCTGGAGGACTAGGCCGTGCAGGACGAGTATTACATGGGGGGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTCCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGAc                                                                                                                                                                                                                                          <  2:257011/237‑1 (MQ=255)
    tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCg                                                                                                                                                                                                                                        >  1:1011555/1‑238 (MQ=255)
     ttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGTCCGAgcgc                                                                                                                                                                                                                                       >  1:11603/1‑238 (MQ=255)
      tGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAGTGCGGCCGc                                                                                                                                                                                                                                       >  2:461136/1‑237 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCACCCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               <  1:28257/239‑1 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAGAAAGCCAAAAGTGCGACACCCTATGTc                                                                                                                                                                                                                                >  2:627929/1‑238 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑AAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTG‑AACCACATG‑CCGAAGTCCACGCGTTGCGTATGGGGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               <  1:304066/239‑1 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTAAATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               <  1:1054063/239‑1 (MQ=255)
            agagATCGCCGGCCGGGAGGACAAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAAGGAGGACGTTTTACCACGCATCCCACCCCGAATGTCGGGTGCGTCATTGT‑CAAAGAGGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACCCGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               <  2:238156/239‑1 (MQ=255)
                   gggggcCGGGAGGACGAAGCCGCGCAGAGCGATTATTACAGGGCGAGGGGGCAAAAGCTGGCACAACGGGGACGTTTTACCACCCATCCCACCCCGAAGGCCGGGTGAGTCTGTGT‑CAAAGATGGCGAAATTCTCGGTGAAGGTTGCCCCCCGCGTGGGGTTA‑AACCACATG‑CCGAAGTACGCGCGTTGCGTATGGCGGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                                        <  2:370111/235‑1 (MQ=255)
                   gcgcgcGGGGGGGAATAAGCCGTGCAGGACGAGTATTACATGGCGCGGGGGCTAAAGCTGGCGCAAAGAGGACGTTTTACCACGCATCACACCCCGAAGGTGGGGGGCGTCATTGA‑CAAAGATGGCGAAATTGTCGGGGAAGGTTACCCCCAACGTGCGGGTA‑AACCACAGG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCg                                                                                                                                                                                                                         <  2:494998/238‑1 (MQ=255)
                    agcgcCAGGAGGGCAAAGCGTTGCAGGCAGGATATTACAGGCCGGGGGCGCTAAGGCTGGCCAACCGAGGCTGTTTTACCACGCATCCCAACCGGAATGTCGGGTGCGTCATGGT‑GAAAGATGGCGAATTTGTGGTTGAAGGTTACCACCACCGGGCGGGGG‑ACCCACATG‑CCGAGTTCCACGCGTTGCGTATGGGGGTTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                                        <  2:1143740/237‑1 (MQ=255)
                        ccTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCCAAAGTGCGGACGccatagttaccctctatcccg                                                                                                                                                                                                                   >  2:286127/1‑220 (MQ=255)
                          tGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGt                                                                                                                                                                                                                 >  1:235064/1‑239 (MQ=255)
                                 cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGCAAAAGACAAACGTGCGAGCGCCTCTCTCACCATCGAACACTGTTACcatc                                                                                                                                                                                                          >  2:1046911/1‑239 (MQ=255)
                                 cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGCGGAAAAGCCCACAGGGCGACCGCCTATGTctccaccggaccctctacccc                                                                                                                                                                                                            >  2:520297/1‑217 (MQ=255)
                                   aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatcat                                                                                                                                                                                                        >  1:166037/1‑239 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               <  2:939015/210‑1 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGGGACCGCCTATGTca                                                                                                                                                                                                                               >  1:939015/1‑210 (MQ=255)
                                           cAGGCCGAGTATGACATGGCGCGGGAGCTAAAGCTGTCGCAACGAGGACTTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑AAAAAATGGCGAAATTGTCGGTGAAGGTTACCCCCACCGTGCGGGTG‑ACCCACATG‑CCGAAGTACCCGCGTTGCGTATGGCGGGTGAAAAATCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTa                                                                                                                                                                                                 <  1:898867/238‑1 (MQ=255)
                                              gACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAAGGGCGCCCGCCTATGTTACACCCGAACCCTGTAGCCATCTTGGTCGTACGc                                                                                                                                                                                              >  1:666115/1‑238 (MQ=255)
                                                cGAGTATTACATGGCGCGGGCGCTAAAGCGGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                            <  1:1017380/238‑1 (MQ=255)
                                                  aGTATTACATGGCGCGGGCGCAAAGGCTGGCGCACCGAGGAGGTTTTACCCCGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAGCGTGCGGGTG‑ACCCACATG‑CCGAAGTACACGCGTTGCGTTTGGCGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAAcc                                                                                                                                                                                                                     <  2:132988/211‑1 (MQ=255)
                                                   gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGAACGCCTATGTCACACTCGGACCCTGTAGCCATCATGGGTGTACGCCACCGt                                                                                                                                                                                        >  1:748447/1‑239 (MQ=255)
                                                     atacaaggcggcgtggcatAAGGCTGGCGAAAGGGGGATGTTGTCCCACGTATCCGAACCCGAAGGGAGGGT‑AGGCAGTGTACAAAAATGGGAAAATTGTCGGTGAGGGTTACCTCCAACGTGGGGGTG‑AACCCTATT‑CGGAAGTATACGGCTGTGGTAAGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                                       <  2:1008014/220‑1 (MQ=255)
                                                     aTTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                                       <  1:1119720/238‑1 (MQ=255)
                                                      ttACAGGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCt                                                                                                                                                                                     <  1:282598/239‑1 (MQ=255)
                                                        aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCCCACTCCAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgt                                                                                                                                                                                   >  1:583713/1‑239 (MQ=255)
                                                         cATGGGGTGGGCGTAAGAGCCGGCGCACCGAGGTCGTTTTCCCACGCATCCCAACCCGAATGTGGGGTGCGCCATCGC‑AAAAGATGGCGAATTTGGCGGAGAAGGTTACCACAAGCGGCGGGGCG‑AACCACTGG‑CCTAAGCACACGCGTGGGGTAGGGCGGGTGAAAAACCCAAGGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgtg                                                                                                                                                                                  <  2:508523/239‑1 (MQ=255)
                                                            ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCcat                                                                                                                                                                                                           >  1:132988/1‑211 (MQ=255)
                                                            ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGTGCGACCCCCCATATCACCCTCGCACCCTGTAGCCATCATGCACGTCCGCCACCGTCCTATGAc                                                                                                                                                                                >  2:1016627/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGGAAAAGCCAAAAGAGCGACCCCCCCTGTTCCACTCCCCCCCTGTAGCCATCATTGTCGAACCCCCCCGTGCtgttgtgg                                                                                                                                                                              >  2:682168/1‑233 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGCAAAAACCAAAGGCGCGACCGCCTATGTCACACTCGAACCCTGTAGCCAACATGGTCGTACGCCACCGTGCTGTGACGc                                                                                                                                                                              >  1:383968/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGc                                                                                                                                                                              >  1:50726/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAAGGGCGGCCGCCTATGTCACACTCCAACCCTGTAGCCATCCTGGTCGTACCCCACCGGGCTGTGAccc                                                                                                                                                                              >  1:640536/1‑236 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAAGTGCGACCGCCTATGTCACACCCGAACCCTGTAGCCACCATGGTCGTACGCCACCGTGCTGTGACGc                                                                                                                                                                              >  1:1101392/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGCGGGAAAAGACCAAAGAGCGGCCCCCCATGTTACACTCGAACCCTCTAGCCAACCTGgacggacgaccacgcccgttgacgc                                                                                                                                                                              >  2:1093124/1‑221 (MQ=255)
                                                                cgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGGCAAAAGGGCGACCGCCTATGTCACACTCGGACCCGGTAACCAGCATGGTCGTACGCCACCCCGCtggggccccc                                                                                                                                                                            >  1:501729/1‑230 (MQ=255)
                                                                cgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAAGCCCCCGTGCTGTGACGCAc                                                                                                                                                                            >  1:898367/1‑238 (MQ=255)
                                                                               gcgcacttcggcatttacaCCACGCCGCCTAACCCGAATGTCGTGGGCTTCAGTGC‑CAGGGAGGGGAAAATTGGCGGGTAGTGTCGCCACCAACGT‑CGGGTGAAACCCGATG‑CAAAAGCAAGCCCGTGGCGTGTGGAGGGTGAAAACGCCAGGGGGACGACCGCCTATGTCACACTCCAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCg                                                                                                                                                             <  2:812224/220‑1 (MQ=255)
                                                                                  accgcagtgggTTTTCCCACGAATCCAAACCCGAAGTGCGGGGGCGGCATTTA‑AAAAGTGGGCGAAATTTCTGGTGAAGGTTACCACACACGTGCGGGTT‑ACCCACATG‑CCGAGGTACCCGGGTTGCGTTTGGGGGCTAAAAAAGCAAAAGGGCCGACCGCCTATGTCACGCTCGAACCCTGTAGCCGTCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAg                                                                                                                                                          <  2:499322/228‑1 (MQ=255)
                                                                                     cGAGGACTTTTTCCCACGCATCCAAACCCGAATGTCGGGGGCGTCATGGT‑CAAAGATGGCGAAATGGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTGGCGTAGGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgcg                                                                                                                                                      <  2:166037/239‑1 (MQ=255)
                                                                                     cGAGGACTGTTTACCACGCACCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGGA‑ACCCACATG‑CCAAAGTACACGCGTTGCGTATTGCGGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgcg                                                                                                                                                      <  2:1053462/239‑1 (MQ=255)
                                                                                         gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATTTCACACTCGAACCCTGTAGCCCTCATGGTCGTACCCCACCCCGCTGTGACGCACTCCTCCCCGCCTGCCTAGcgcgcggg                                                                                                                                                  >  1:51836/1‑237 (MQ=255)
                                                                                                    aCGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGAt                                                                                                                                       >  1:1114014/1‑239 (MQ=255)
                                                                                                       cacccaaaCCCTAAGTTCGGGGGCGACATTGC‑CAAAGATGGGGAAATTGGCGGTGAAGGTTACCACCAGCGGGCGGGGG‑AACCACATG‑CCGAATGACCCGGGTTGCGTATGTGGGGGAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                    <  2:50726/233‑1 (MQ=255)
                                                                                                       cATCCCACCCCGAATGTCGGGTGCGTCATTGT‑AAAAGATGGCGAAATTGTCGGTGAAGGTTCCCCCCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACAGGCTTTGGGTAGGGGGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                                       <  1:1123407/188‑1 (MQ=255)
                                                                                                       cATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                    >  1:426231/1‑239 (MQ=255)
                                                                                                       cATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCCATGTCACACTCCAACCCTGTAGCCATCATGGGCGTACGCCCCCCTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGagtca                                                                                                                                    >  1:1092912/1‑235 (MQ=255)
                                                                                                        ttCCCAACCAGAATGTCGGGGGCGTCATGGT‑CAAAGATGGCGAAAGTGTCGGTGAAGGTTACCACCAACGTGCGGGGG‑AACCACCGG‑CCAAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                    <  2:235064/237‑1 (MQ=255)
                                                                                                        accccAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATGGTCGGTGAAGGTTCCCCCCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCTTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                    <  1:172260/236‑1 (MQ=255)
                                                                                                              aCCCGAATGTGGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTAGGGCGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                                                                                                                             <  1:682168/239‑1 (MQ=255)
                                                                                                                    aTGTCGGGAGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAACGCGCGGCCGCCCATGTCAAGCTACCACCCCATAGCCATCATGGTCGGCCGGCCCCGATGTGTGACGCCCCCCTCCCCGCCGGCGTAGCGCGCGTGGTGTGCTCGGTGCCAGATACTAAcca                                                                                                                        >  2:688027/1‑237 (MQ=255)
                                                                                                                       tCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGaa                                                                                                                                                                                                                       <  1:726195/140‑1 (MQ=255)
                                                                                                                          ggTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGGCAAAGGTGCGACCGACTATGTCACACTCGCACCCTGTCACCATCATGGTCCTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCGAGATCCTCACCCGCAGGTc                                                                                                                 >  1:139028/1‑239 (MQ=255)
                                                                                                                          ggTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               >  2:119888/1‑129 (MQ=255)
                                                                                                                             gCGTCTTTGT‑CAAAGAGGGCGAAATTGTCGGTGAAGTTTCCCACCAACGTGCGGGTG‑ACCCACATG‑CCGAAGTACGCGCGTGGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc                                                                                                               <  1:263244/238‑1 (MQ=255)
                                                                                                                             gCGTCATTGT‑CAAAGATGGCAAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTG‑ACCCACATG‑CCGAAGTACACGCGTTGCTTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc                                                                                                               <  1:61399/238‑1 (MQ=255)
                                                                                                                                 cATTGT‑CAAAGATGGCGAATTGGTGGGTGAAGGTCCCCCCCAACGTGCGGGGG‑AACCCAATG‑CCGAAGTACGCGCGTGGCGTATGGGGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                          <  1:344902/239‑1 (MQ=255)
                                                                                                                                 cATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCAAAAGGGGGACCGCCTATGTGACACTCGAACCCTGTAGcc                                                                                                                                                                                                             >  2:726195/1‑140 (MQ=255)
                                                                                                                                 aaTTGT‑CAAAGAGGGCGAAATTGTCGGTGAGGGTACCCCCCAAGGGGGGGGTG‑AACCACAGG‑CCGAATTACACGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                          <  2:898367/238‑1 (MQ=255)
                                                                                                                                   ttGC‑CAAAGATGGCAAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt                                                                                                        <  1:778781/239‑1 (MQ=255)
                                                                                                                                    tggtaaaggATGGGAAAAGTGTGGGGGAAGGTTACCACCAA‑GTTGGGGTA‑GACCTCACGCCCGAGGAACACGCGTGCCGTTGGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGGTCCTAACCCGCAGGTCGCTGGGCGt                                                                                                        <  2:276905/231‑1 (MQ=255)
                                                                                                                                              tGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTa                                                                                                <  1:1158699/237‑1 (MQ=255)
                                                                                                                                              gggCAAAAGTTTCTGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTa                                                                                                <  1:1128897/236‑1 (MQ=255)
                                                                                                                                                      ttGTCGGTGAAGGTTACCACCACCGTGCGGGTG‑ACCCACATG‑CCGAAGTACACGCGTTGCGTATGGGGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCaa                                                                                      <  1:1093124/239‑1 (MQ=255)
                                                                                                                                                                       ccccaCCGGGCGGGTG‑AACCCCATG‑CCGAAGTCCACGCGTGGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                                     <  1:313714/237‑1 (MQ=255)
                                                                                                                                                                            aCGTGCGGGTG‑AACCACATG‑CCGAAGTACATGCGTTGCGTATGGCGGGTGAAAAACCCAAAAGTGCGACCGCCCATGTTACAACCGACCCCCGTAGCCATCACGTTCGTACGCCACCGTGCTGTGACGCACCCACAGCCCCTGGCGTACCGCGCGTGGATGCCTCGAGGCAACATCCTAACACGCTGGTTGCTGGGCCGGGACTTTTACGTCTGCAACACGCTGGCACTGACGTCAGc                                                                 >  2:391060/1‑238 (MQ=255)
                                                                                                                                                                            aCGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTTGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGTGGACCTTACCGTCTGCCACAAGCTGGCCTTGACGTCAGc                                                                 >  1:545250/1‑238 (MQ=255)
                                                                                                                                                                             cGTGCGGGTG‑ACCCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                               <  1:1046911/239‑1 (MQ=255)
                                                                                                                                                                             cGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCCCACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                                                                                                                          >  1:631701/1‑180 (MQ=255)
                                                                                                                                                                               ggtcggttaacacacaCG‑CCGAAGTTCGGGCGTTTGGTAAGGCGGGGAAAAAGGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTTCGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                               <  2:426231/226‑1 (MQ=255)
                                                                                                                                                                                gCGGGTG‑AACCACATG‑CCGAAGTCCCCGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                             <  1:512267/238‑1 (MQ=255)
                                                                                                                                                                                 cGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGCGAAAAAGCCAAAGGCGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTTGATGCAAGATCCTAAc                                                                                                                          >  1:555359/1‑176 (MQ=255)
                                                                                                                                                                                        aaCCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTCTGTCACACCCGAACCCTCTAGCCATAATTGTCGTACGCCACCGTGCTGGGACGCACTCCCCGCCCCTCGCGCAGCGCGCGGGGATGGCTCGATGCAAAAACCCAACCCGCCGGCCGCCGGGCGGGGACGCTACACTCTGGAACAGGCTGGCCTTGTCGTCAGTCACGGCCtggtg                                                     >  2:694464/1‑239 (MQ=255)
                                                                                                                                                                                         aCCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                     <  1:461896/238‑1 (MQ=255)
                                                                                                                                                                                          ccACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCCAAGGGGCGACCGCCTATGTCACACTCGCACCCTGTAGCCACCATGGGCGTACGCCCCCGCGCTGTGACGCACCCATCGCCGCTGGCGTAGCGCGCGTGGGTTCCTCGCTGCAAGATCCTAACCCGCAGGGCGCGGGGCGGGGGCTTTACCGTCTGCAACAGGCTGGCCTTTACGTCCACCCCGGCCtgttggt                                                   >  2:408165/1‑237 (MQ=255)
                                                                                                                                                                                             aaTG‑CCAAAGTACACGAGTTGCGAAGGGCGGGTGAAAAAACCAAAGGTGCAACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAg                                                <  1:239688/238‑1 (MQ=255)
                                                                                                                                                                                                     aaGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGcc                                         >  1:117709/1‑239 (MQ=255)
                                                                                                                                                                                                      aGTACGCGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                        <  1:520297/239‑1 (MQ=255)
                                                                                                                                                                                                      aGTACACGGTTGGCTAATGGGGGGTGAAAAAGCAAAAGGTGCCACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                        <  1:712891/239‑1 (MQ=255)
                                                                                                                                                                                                        tccacGGGTTGCGTATGGGGGGGGAAAAACGCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                      <  2:583713/237‑1 (MQ=255)
                                                                                                                                                                                                         tcccgctTTGCGTATGGCGGTTAAAAAATCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTTCGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                      <  1:203998/235‑1 (MQ=255)
                                                                                                                                                                                                         acacGCGTTGCGTATGGCGGGAGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                      <  1:286127/238‑1 (MQ=255)
                                                                                                                                                                                                          cacGCGTTGCGTATGGCGGGTGAAAAAGCCAAAAGTGCGACCGCCTATGTCACACTCGGACCCTGTAGACATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCCCCGCCGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTCACCCGCAGGGCGCTGCGCGCGGACGTTACCGTCTCCAACAGGCCGTCCTTGACGCCAGCCACGGCCCGATGATGAggggagacgcgga                                    >  2:899172/1‑227 (MQ=255)
                                                                                                                                                                                                           ccgcgTGGCGTATGCAGTGTGAAAAAGCCAAAGGGGCGTCCGCCTATGTCACACTCGAACCCGGTAGCCATCATGGTCGTACGCCGCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGACACGGCCTGATGATGAGTGAAGCCGAGCaa                                   <  2:139028/238‑1 (MQ=255)
                                                                                                                                                                                                           acGCGTTGGGTATGGCGGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa                                   <  1:707734/239‑1 (MQ=255)
                                                                                                                                                                                                           acGCGTTGCGTATGGCGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa                                   <  1:627929/239‑1 (MQ=255)
                                                                                                                                                                                                             gggtggCGTATGCGGGGCTAAAAAGTGAAAGGTGCGTCGGCCTTGGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGGGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAAt                                  <  2:640536/233‑1 (MQ=255)
                                                                                                                                                                                                                  gCGTATGGCGGGTGAAAAAGCCAAAAGTGCGGCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGGGCCGCACTCATCCCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCCGGTCGCTGGGCGTGGGCTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCCCCCCCTGCTAATGATGGACGCCGAGCACTTGAATa                            >  2:1079214/1‑239 (MQ=255)
                                                                                                                                                                                                                   cGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                          <  1:370340/160‑1 (MQ=255)
                                                                                                                                                                                                                     tATGGCGGGTGAAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCCGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAg                         >  1:794886/1‑239 (MQ=255)
                                                                                                                                                                                                                             gTGAAAAAGCCAAAGGTGCGACCCCCTATGTCCCACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGCTGCCTCGCTGCACGACCCTAACCCCCAGGTCGCTGGGCGGGGCCTTTACCGTCTTCAACAGGCTGGCATTGACGTGCGCCACGGCCTGGTGATGAGTGAGGCCGAGCAATTGACTAAAGGCTTtttc                 >  2:558232/1‑239 (MQ=255)
                                                                                                                                                                                                                             gTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCCGGTCGCTGGGCGTGGACTTTAcc                                                                                              >  1:656933/1‑162 (MQ=255)
                                                                                                                                                                                                                                aaaaaGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAg              <  1:1031786/239‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcaaaaGGGGCCACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGa          <  2:1114014/235‑1 (MQ=255)
                                                                                                                                                                                                                                       cAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGAt         <  1:499793/237‑1 (MQ=255)
                                                                                                                                                                                                                                            gtgtGACCGCCGATGTCACACTCAATCCCTTTTGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTATCCCGCAGGGCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCTCGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAc   <  2:859233/238‑1 (MQ=255)
                                                                                                                                                                                                                                             tGCGACCGCCTATGTCCCACTCGACCCCTGTCGCCATCATGGTCGTACGCCTCCGTGCTGTGACGTACTCATCGCCGCGGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAcc  <  2:1147623/238‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
AGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGT‑CAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTG‑AACCACATG‑CCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCACC  >  NC_000913/433415‑433886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A