Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F9 I0 R1 105 139.1 2894862 97.5% 2822490 224.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 433,649 T→G 19.9% G65G (GGT→GGG)  ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913433,6490TG19.9% 152.7 / 11.9 119G65G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (29/59);  new base G (22/3);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 1.47e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

Only 100 of 119 total aligned reads displayed.

AGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCA  >  NC_000913/433415‑433884
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
aGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTCCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTAAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGa                                                                                                                                                                                                                                          <  1:856104/239‑1 (MQ=255)
aGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGa                                                                                                                                                                                                                                          <  1:372640/239‑1 (MQ=255)
    tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGGG‑CGAccc                                                                                                                                                                                                                                       >  1:1301732/1‑237 (MQ=255)
            agagGTCGGGCGCCTGGGGGACTGAGCCGTGCAGGACGATGATTACATGGCGCGGGGTGTAAAGCTGGCGCAACGAGGACTTTTTACCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTca                                                                                                                                                                                                                              <  2:1186783/239‑1 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTCCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCAACAAAGTGCGGGGGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGA‑GGAAAAAACG‑AAAGATg‑agagctgctctgtac                                                                                                                                                                                                                              >  2:220092/1‑227 (MQ=255)
                        ccTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGAC‑AAAGGCG‑CGAcacgccattgcccaccccaaccc                                                                                                                                                                                                                   >  2:790588/1‑216 (MQ=255)
                          tGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGt                                                                                                                                                                                                                <  1:287213/239‑1 (MQ=255)
                                 cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAAGGG‑CGACCGCCTATGTCAAACTCGAACCCTGTAGccct                                                                                                                                                                                                          >  1:1379033/1‑236 (MQ=255)
                                   aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAACC‑AAAAGGG‑CGGCCGCCTATGTCACACTCGAACCCTGTAGCcat                                                                                                                                                                                                          >  1:1373009/1‑236 (MQ=255)
                                   aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGCGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGG‑TGCGTATGGCGGG‑GGCAAAAGAC‑AAAGGGG‑CGCCCGCCTACGTCGCACTTCCACCCTGTACCcctccc                                                                                                                                                                                                       >  2:333410/1‑237 (MQ=255)
                                       cGTGCAGGACGAGTATTACAGGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATgg                                                                                                                                                                                                     <  1:1416032/237‑1 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGGAAAAGAC‑AAAGGTG‑CGACCCCCTATGTCCCACACCAACCCAGTAGCCATCATGGTCGt                                                                                                                                                                                                 >  2:511806/1‑239 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGGAAAAGCC‑CAAGGGG‑GGAACGCCTCTGTCCCAACCGAACCCCGTAGcccaccctgtcg                                                                                                                                                                                                  >  2:1381815/1‑228 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGCCCGCCCATGTCACACCCGAACCCTGTGGCCCTCATGGTCGt                                                                                                                                                                                                 >  1:1327256/1‑239 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGGG‑CGGCCGCCTATGTCACACTCGCACCCCGTCGGCCACATGGTCGt                                                                                                                                                                                                 >  1:448130/1‑239 (MQ=255)
                                         tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGt                                                                                                                                                                                                 >  1:1445828/1‑239 (MQ=255)
                                          gCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGGACAAACC‑AAAGGGG‑CGACCGACCATGTGACACTCGAACCCTCTAGCCATCATGGGCGt                                                                                                                                                                                                 >  2:480714/1‑238 (MQ=255)
                                          gCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGAC‑AAAAGGG‑CGACCGCCTATGTTACACTCGAACCCTGTTGCCATCATGGTCGt                                                                                                                                                                                                 >  1:1097166/1‑238 (MQ=255)
                                              gACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCTAAACCTGTTGCCATCATGGTCGTACGc                                                                                                                                                                                             >  1:109149/1‑238 (MQ=255)
                                              gACGAGTATTACAGGGCGCGGGCGCTAAGGCGGGCGAAACGAGGACGTTTTCCCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGGCGGTGAAGGTTACCACCAACGTGGGGGGGAACCACAGGCCGAAGTCCACGCGT‑TGCGTAGGGCGGG‑TGAAAAAGCA‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGc                                                                                                                                                                                             <  2:394246/238‑1 (MQ=255)
                                                cgatttttACATGGGGCGGGCGCTAAGGCTGGCGCAACGAGGCGGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGT‑TTCGTATGGGGGG‑TAAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                           <  2:1445828/232‑1 (MQ=255)
                                                cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACTTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGTTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                           <  1:405618/238‑1 (MQ=255)
                                                cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACAGGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                           <  1:44865/238‑1 (MQ=255)
                                                cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCa                                                                                                                                                                                           >  1:1163975/1‑238 (MQ=255)
                                                   gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGACATTGTCGGTGAAGGTTACCACCAACGTGCGGGAGAACCACATGCCGAAGTCCACGCGT‑TGCGTATGGCGGC‑GGGAAAAGCC‑AAAGGAG‑TGCACGCCCACGTCCAACTCGATCCCGGCTGCCATCATGTTccagacccaccgg                                                                                                                                                                                       >  2:1266673/1‑227 (MQ=255)
                                                   gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑Gt                                                                                                                                                                                      >  1:295017/1‑239 (MQ=255)
                                                    tATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCAAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGTTGAACCACATGCCGAAGTACACGCGT‑TGCTTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCcat                                                                                                                                                                                                          <  1:160824/219‑1 (MQ=255)
                                                      ttccagggggggggCGCAAAGGCTGGCGCAACGGGGCCGTTTTCCCACGCATCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTAGGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCt                                                                                                                                                                                   <  2:295017/228‑1 (MQ=255)
                                                        aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCtgt                                                                                                                                                                                 >  1:517996/1‑239 (MQ=255)
                                                        aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACCCGAACCCTGTAGCCATCATTGGCGTACGCCAAC‑GTgtcgt                                                                                                                                                                                 >  1:818379/1‑235 (MQ=255)
                                                            ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑GGACCGCCCATGTCACAACCGAACCCTGTAGCCCTCATGGTCGTACGCCACC‑GAGCTGTGAc                                                                                                                                                                              >  1:276815/1‑238 (MQ=255)
                                                             tggcgGGGGCAAAGGCTGGCGCAACGAGGAGGTGTTTCCAGGCATCCCAACCCGAATGTCGGGTGCGTAATTGCAAAAGATGGCGAAATGGTGGGTGAAGGTTCCCACCAAGGGGCGGGTGAACCACATGCCGAAGTACACGCGT‑GGCGTATGGGGGG‑TGAAAAAGCA‑AAGGTTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACg                                                                                                                                                                             <  2:756596/236‑1 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGTCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑CAAGGGG‑CGAACGCCTATGTAACACTCCAACCCTGTAGCCATCATGGGCGTACGCCCCC‑GTGcccgtctctt                                                                                                                                                                           >  1:438791/1‑230 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGTTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGc                                                                                                                                                                            <  1:654316/238‑1 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGc                                                                                                                                                                            >  1:1348777/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑AGACCGCCTATGTCACACTCGAACCCCGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGc                                                                                                                                                                            >  1:1278632/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCCCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGc                                                                                                                                                                            >  1:681579/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAACC‑AAAGGGG‑CGACCGCCTATGTCACCCTCGCACCCCGAGACCACCACAGTCGTACGCCAccccgcctggacgc                                                                                                                                                                             >  2:1432134/1‑226 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGGGAAAGCC‑CAAGGGG‑CGACCGCCCATGTCACACACGCACCCTGTAGCCACCATGGTCGGAAGCCACC‑GTGCTCTGAccc                                                                                                                                                                            >  2:1348486/1‑236 (MQ=255)
                                                                cgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCAACATGGTCGTACCCCACC‑GTGCTGTGACGCAc                                                                                                                                                                          >  1:1140182/1‑238 (MQ=255)
                                                                   gcgcTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑CAAGGTG‑CGACAGCCTATTTCACACCCGAACCCCCTAGCCATCATGGTCGTACGCCACC‑GTGCCGTTACCCCCTCa                                                                                                                                                                       >  1:465879/1‑238 (MQ=255)
                                                                   gcgcTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GTGAAAAGAC‑ACAAGTG‑GGGCCGCCCCACTCACAACCCGACCCTTTACCCATCATGGTGGTCCggcccccggctctggctccaccc                                                                                                                                                                        >  2:1245806/1‑216 (MQ=255)
                                                                     gcTAAAGCTGGAGCAACGAGGACGTTTTACCACGCATCCCGACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGT‑TGCGTATGGCGGG‑TAAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATc                                                                                                                                                                     <  2:707614/238‑1 (MQ=255)
                                                                        aatgcgggccaaaCAAGGACGTTTTTCCGCAAATCCCACCCAGAATGGCGGGTGCTTCATTGTAAAAGTGGGCGAATTGGTCGGTGAAGGTTACCACCAACGTGGGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCA‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATcgccg                                                                                                                                                                 <  2:465879/228‑1 (MQ=255)
                                                                               gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGGAAAAGCC‑AAAAGGG‑CGACCGCCTATGTCACACTCGGAACCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGt                                                                                                                                                          >  1:33204/1‑239 (MQ=255)
                                                                                 gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCGCACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAg                                                                                                                                                        >  1:818173/1‑239 (MQ=255)
                                                                                     cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAAGTG‑CGACCCCCTATGTCACACTCGAACCCTGTAGGCATCATGGTCGTAACCCACC‑GGGCTGTGACGCACTCCTTGCCCCCGGccgaacccc                                                                                                                                                    >  1:623695/1‑231 (MQ=255)
                                                                                     cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCCCCTATGTCACACCCGAACCCTGTAGCCCACATGGTCGTACGCCACC‑CTGCTCTGACGCCCCCATCCCCCCCGGCGTAgcgc                                                                                                                                                     >  2:347566/1‑238 (MQ=255)
                                                                                         gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAACC‑AAAAGGG‑CGACCGCCTTTGTTACAATCGAACCCTGTAGCCATCATGGCCGTACGCCCCC‑CGGCTGTGACGCCATCATcgccgcacggggagggttgtt                                                                                                                                                 >  2:1118776/1‑223 (MQ=255)
                                                                                                 cccACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                                                                                                                        <  1:1381815/238‑1 (MQ=255)
                                                                                                 aCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTTAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAACC‑AAAGGAG‑CGACCGCCTATGTCACACTCGCACCCTGTAGCCATCCTAGACGTCCACCCCCTATGC‑GTCACGCACTCCTCACCGCCCGCGCACCGCGCATCGTTTccc                                                                                                                                         >  2:788333/1‑237 (MQ=255)
                                                                                                        accccAACCCGAATGTCGGGTGCGTCATGGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGGGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAg                                                                                                                                                        <  1:595020/214‑1 (MQ=255)
                                                                                                        aTCCGACCCCGAATCGCGGGGGGGTCATGGTAAAAGGGGGCGAAATTGTCGGTGAGGGTTCCCACCACCGTGCGGGTGACCCACATGCAAAAGAACACGCTT‑TCGGTAGGGCGGT‑TGAAAAACCA‑AAAGGGG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGGCTCGATGCa                                                                                                                                  <  2:109227/238‑1 (MQ=255)
                                                                                                        aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                  <  1:1252257/238‑1 (MQ=255)
                                                                                                        aTCACAACCAGAATGTCGGGTGCGTCATGGTGAAGGAGGGCGAAATTGTCGGTGGAGGTTCCCGCCAGGGTGCGGGTGAACAACAGGCCGAAGTACACGCGT‑GTCGTAGGGCGGG‑TGAAAAAGCC‑AAGGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                  <  2:635174/238‑1 (MQ=255)
                                                                                                          cccAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGa                                                                                                                               >  1:421932/1‑239 (MQ=255)
                                                                                                                    aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAccc                                                                                                                      >  1:900534/1‑238 (MQ=255)
                                                                                                                    aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAACC‑CAAGGTG‑CGACCCCCTATGTCAAACTCGAACCCTGTAGCCCTCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCCCCGGCGTAGCGCGCGTGGTTGCCTCGCTTCAAGCTCCTAAccc                                                                                                                      >  1:67006/1‑238 (MQ=255)
                                                                                                                     tgtggggtgctcaatttgaaaaGGTGGCAAAATTGGCGGGGAAGGTTACCACCAAGGGGGGGGGGAACCACATGCAGAAGTACCCGCGT‑TGCGTATGGGGGG‑TGAAAAGGCA‑AAGGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc                                                                                                                    <  2:653218/220‑1 (MQ=255)
                                                                                                                           gTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTc                                                                                                               >  1:1296929/1‑238 (MQ=255)
                                                                                                                             gggtaatgggcagagagGGTGAAATGGTCGGTGAGGTTCTCCCCGAACGTGGGGGTCATCCACATGTCCAATGCCACGGGTGGGCGT‑TGGGGGG‑TGAAAGACCA‑AATGGTG‑GCACCGCTGATGTCACCCTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc                                                                                                             <  2:420073/225‑1 (MQ=255)
                                                                                                                             gCGTCATTGTAAAGGATGGAGAAATGGTCGGTGAGGGTTCCCACCAACGTGGGGGTGAACCACACGCCGAAGCACCCGCGT‑TGCGTATGGCGGG‑GAAAAAAGCA‑AAGGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc                                                                                                             <  2:639114/238‑1 (MQ=255)
                                                                                                                             gCGTCATTGTAAAAGATGGCAAAATTGTCGGTGAAGTTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCt                                                                                                            <  1:1079655/239‑1 (MQ=255)
                                                                                                                                 cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                        <  1:1416917/239‑1 (MQ=255)
                                                                                                                                 cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTAGGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                        <  1:593349/239‑1 (MQ=255)
                                                                                                                                   ttGTAAAAGATGGGAAAATTGTCGGGGAGGGTTCCCACCAACGTGCGGGTGAACCACATGCCAAAGTACACGCGT‑TGCGTAGGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt                                                                                                      <  1:194913/239‑1 (MQ=255)
                                                                                                                                             tGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACAGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTa                                                                                              <  1:1312031/237‑1 (MQ=255)
                                                                                                                                                caaaaTTGTCGGTGAGGGTTCACACCAGCGTGCGGGGGAACCAAATGCCGAATTACCGGCGT‑TTCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGt                                                                                          <  1:1052038/236‑1 (MQ=255)
                                                                                                                                                cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCg                                                                                           >  1:860921/1‑237 (MQ=255)
                                                                                                                                                     ttGTGGGTGAAGGTTACCACCAACGTGGGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGGGGA‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCaa                                                                                    <  1:702919/239‑1 (MQ=255)
                                                                                                                                                       gTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAACCATCATGGTCGTAAGCCACC‑GGGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGGGGACTTTACCGACTGCAACa                                                                                  >  1:117951/1‑239 (MQ=255)
                                                                                                                                                         cGGTGAAGGTTCCCCCCAACGTGCGGGTGAACCACATGCCGAAGTACGCGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAgg                                                                                <  1:1032067/239‑1 (MQ=255)
                                                                                                                                                                      ccccacgcggCGGGTAAACCATGTGCCCAGGTACGCG‑TT‑TTCGTTCGGGGGGTTGAAAAAGCC‑‑AAGGTGACGTCCCCCTAGGTCACACGCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCGTGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                                   <  2:1097166/230‑1 (MQ=255)
                                                                                                                                                                      cccaaaCGTGGGGGTGACCCAAAGGCCGAGGTACACGCGT‑GGCGTATGGGGGG‑TAAAAAACCA‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                                   <  2:1348777/235‑1 (MQ=255)
                                                                                                                                                                           aCGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCGGGCGTAGCGCGCGTGGTTGCCTCGGTGCAAGATCCTAACCCGCAGGGCGCCGGGGGTGGACTTTTCCGTCCGCAACAGGGTGGCATTgcgcgcgtg                                                               >  2:804254/1‑230 (MQ=255)
                                                                                                                                                                           aCGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTTACGTCAGc                                                               >  1:19190/1‑238 (MQ=255)
                                                                                                                                                                            cgggCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTAGGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                             <  1:347566/236‑1 (MQ=255)
                                                                                                                                                                            cGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                             <  1:492281/239‑1 (MQ=255)
                                                                                                                                                                             gggCGGGTGACCCATGGGCCGAGGTGCCCGGTT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGCTG‑CGCCCGCCTATGTCACACTCGAACCCTGTAGCCAGCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGTCATTGACGTCAGCCa                                                             <  2:192714/236‑1 (MQ=255)
                                                                                                                                                                              gccGGGTGACCCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                             <  1:1083995/235‑1 (MQ=255)
                                                                                                                                                                                cGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑CAAAGAG‑CGACCCCCTCTGTCCCAATCCAACCCTTTACCCATCACGGTCGAACGCCCCC‑GCCCTGCGCCGCCCGCCGCGCCGCTGCCCGAGCGCGCCGGGGTGCCCCGGCGGAAGATCCTACCCCGCACCGCGCTGGGCGCGCGCTTTACCGTCGTCAAAAGGCTGGCATTTCCGTCCGcccccg                                                          >  2:1287110/1‑234 (MQ=255)
                                                                                                                                                                                 gggTCAACCACATGCGTAAGAACACGCTT‑CTCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTC‑CGCCCGCCTATGTCACACTCGAACCCTGTAGCCAGCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                                         <  2:33204/238‑1 (MQ=255)
                                                                                                                                                                                       aCCAAATGCCGAAGTACACGCGT‑TGCGTAGGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                   <  1:360012/238‑1 (MQ=255)
                                                                                                                                                                                             tGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTg                                            >  1:923733/1‑239 (MQ=255)
                                                                                                                                                                                                caaagtcctgtggT‑TGCGTATGGGGGG‑TGAAAAAGCA‑AAAGGTC‑CGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg                                         <  2:181230/227‑1 (MQ=255)
                                                                                                                                                                                                cGAAGTACCCGGGT‑TGCGTATGGCGGG‑TAAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg                                         <  1:1266673/239‑1 (MQ=255)
                                                                                                                                                                                                cGAAGTACACGCGT‑TGCGTATGGCGTG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg                                         <  1:530144/239‑1 (MQ=255)
                                                                                                                                                                                                cGAAGTACACGCGT‑TGCGTAGGGCGGG‑GAAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg                                         <  2:361392/239‑1 (MQ=255)
                                                                                                                                                                                                   aGTACACGCGT‑TGCGTATGGCGGG‑GGAAAAAACC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGGCACC‑CGGCTGTGACGCCCTCATCCCCCCCGGCGGAGGGGGCGGGGTTGCCTCGGTGGAAGGATCTAACCCGCCGGGCGCCGGGCGGGGGCCTTCACGTCTGCAAACGGCTGGGCGTGGCCTCCGCCACgggcgtgtggtgaggggagccg                                      >  2:1217390/1‑219 (MQ=255)
                                                                                                                                                                                                     tACACGCGT‑TGCGTATGGCGTG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                                         <  1:1442821/218‑1 (MQ=255)
                                                                                                                                                                                                      acacGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                    <  1:31945/238‑1 (MQ=255)
                                                                                                                                                                                                        acGCGT‑TGCGTATGGCGGG‑GGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCCGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCCCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGGGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa                                 >  2:512630/1‑239 (MQ=255)
                                                                                                                                                                                                                gCGTATGGCGGG‑TGAAAAAGCC‑AAAGGGG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGGCTGATGATGAGTGAAGCCGGGCAATTGAATa                          >  1:553342/1‑239 (MQ=255)
                                                                                                                                                                                                                 gcgaaggggggctGAAAAACCC‑AAAGTTG‑GCACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGGCTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATa                          <  2:109149/227‑1 (MQ=255)
                                                                                                                                                                                                                 cttaggggggg‑TGAAAAACCC‑AAGGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATa                          <  2:553342/230‑1 (MQ=255)
                                                                                                                                                                                                                         ggg‑TGTAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCACGGTCGTACGCCACC‑GTGCTGTGACCCACTCATCCCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGGCAGCCACGGCCTGATGATGAGTGAAGCCGCGCACTTGGATAAAGGCtt                   >  2:527026/1‑237 (MQ=255)
                                                                                                                                                                                                                               aaaaaGTC‑AAGTGCG‑CGTCCGCCGATGTCACACGCGAACCCTGTAGCCATCAT‑GTCGTACGCCGCC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGc           <  2:848759/239‑1 (MQ=255)
                                                                                                                                                                                                                                aaaaGCCAAAAGGTG‑CACCTGCCTATGTCACGCTCGAACCCTGTAGCCATCATGGTCGTCCGCCCCC‑GTGCTGTGACGCACTCATCCCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAg            <  2:201398/239‑1 (MQ=255)
                                                                                                                                                                                                                                         aaGGGG‑CGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTTCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATgcgc   <  1:790616/239‑1 (MQ=255)
                                                                                                                                                                                                                                          aGGTG‑CGACCGACTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGGCGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCa  <  1:369565/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
AGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGT‑TGCGTATGGCGGG‑TGAAAAAGCC‑AAAGGTG‑CGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACC‑GTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCA  >  NC_000913/433415‑433884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A