Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F17 I0 R1
|
19 |
52.5 |
1051376 |
98.5% |
1035605 |
229.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
433,649 |
T→G |
29.9% |
G65G (GGT→GGG) |
ribD → |
fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 433,649 | 0 | T | G | 29.9%
| 22.4
/ 3.1
| 32 | G65G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
| Reads supporting (aligned to +/- strand): ref base T (6/16); new base G (6/4); total (12/20) |
| Fisher's exact test for biased strand distribution p-value = 1.19e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01 |
TTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCT > NC_000913/433419‑433870
|
tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGAACg > 1:370558/1‑238 (MQ=255)
aaGAGAACGCGCGCATGAAGGACTAAGCCGTGCGGGACGGGTATTACATGGCGGGGGCGCTAAAGCTGGCGCCAAGAGGACGTTTTACCACGAATCCCAACCCGAGTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAAGGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGt < 2:70904/238‑1 (MQ=255)
ggaggaCAAAGCCGTGCAGGACGAGTATGCCAGGGCGCGGGCTGTAAAGCGGGCGCAACGAGGCCGTTTTCCCACGCTACCCACCCCGAATGTCGGGGGCGTCATTGTGAAAGATGGCGAAATTGTCGGTGAAGTTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGt < 2:350628/238‑1 (MQ=255)
cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGGGGACGCCCCAGTCACCCCCGCACCCTTTAGCcat > 2:287053/1‑238 (MQ=255)
cggtgaGGACGAGTATTGCAGGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTGGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTc < 2:166748/234‑1 (MQ=255)
gaGGACGAGTTTTACATGGCGGGGGCGCTAAAGCGGGCACAACGAGGACGTTTTACCACGCACCCCAACCCGAATGCCGGGTGCGTCATTGTCAAAGATGCCAAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTCCACGCGTTGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTa < 2:255401/237‑1 (MQ=255)
gACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGCCAAAGGGGCGACCGCCTATGGTACAATCGAACCCTGTAGCCATCATGGTCGTACGc > 1:337784/1‑238 (MQ=255)
cgggtcttACAGGGCGCGGGCCGTAAAGCGGGCGAACAGAGGGCGTTTCACCCCGCATCCCACCCGGAATGGCGGGGGCGTCATTGTCAAAGATGGCAAAATGGTCGGTGAAGGTTTCCACAAACGTGGGGGTGACCCACAGGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa < 2:391009/232‑1 (MQ=255)
gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGCCAAAGGTGCGACCGCCGATGTCACACTCGAACCCCGTAGCCATCATGGGCGTGAGCCACCgg > 1:211973/1‑238 (MQ=255)
tATTACATGGGGCGGGCGTTAAAGCGGGCGCAACGAGGACGTTTTACCACGCAGCCCAACCCGAATGTCGGGGGCGCAATTGACAAAGAGGGCAAAATTGTGGGGGAAGGTTACCCCCAACGTGCGGGTGAACCACAGGCCGAGGTACACGGGTGGCGTATGGCGGGGGAAAAACCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg < 2:262926/239‑1 (MQ=255)
aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGCGGAAAAAACCAAAGGGGCGGCCGCCTATGTTCCACTCGGACCCTGTAGGCACCAACGGCGCACGCCACCGTGCtgt > 1:68769/1‑239 (MQ=255)
cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCCCACTCGAACCCTGTAGCCATCCTTGTCGTACGCCCCCGTGCTGTGACGc > 2:178563/1‑238 (MQ=255)
ccTAAAGCTGGCGAAACGAGGACGTTTTACCACGCATCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTAACCCACATGCCGAAGTACACGCGTGGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc < 2:383641/237‑1 (MQ=255)
tACCAAGCATCCTACCCCGAATGTCGGGTGCGTCATTGTCAAGGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCt < 2:333928/239‑1 (MQ=255)
ccACGCACCCCAACCCGAATGGCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCg < 2:245655/239‑1 (MQ=255)
cATCCCAACCCGAAGGTGGGGGGCGGCATTGTCAAAGAGGGCGAAATTGGCGTGGAAGGTTCCCCCCAGCGTGCGGGTGAACCCAATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa < 2:337784/239‑1 (MQ=255)
aacccagcgggAATGTGGGGTGCGTAATGGTCAAAGATGGCGAAGTTGTGGGTTAAGTTTACCACAAAGGTGCGGGTGAACCACATGCGGAAGTACACGCGTTGCGTATGGCGGGTGAAAAGGCCAATGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa < 2:68769/228‑1 (MQ=255)
tCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCACAAGGGCGCCCCCCCCCGGCACCCTCTGACCCTCTACCCCTCATGGGCGTCCGCCCCCGCCCTGTGACCCCCTCCGCGCCCCCGCCGTGGAGCGCGCGGTTACCTcccctgacg > 2:254920/1‑231 (MQ=255)
gtggggTGCGGAATTGTAAAAGGTGGCAAAATTGGCGGTGAGGTTTACCGCCACCGTGCGGGTGAACCACATGCGGAAGTCCCCGCGTGGCGTAGGGGGGGTGAAAAAGCCAAGGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc < 2:132066/235‑1 (MQ=255)
cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCCCCTATGTCACACCCGAAACCTGTAGCCCTCATGGGCGTACGCCACCGTGCTGTGACGCACTCATTCCCGCCCGCGTCACGCGCGTGGTTGCCTCGATGCCAGAACCTAACCCCCGGGTGCCTGGGCGTGGaacttaacct > 2:2056/1‑229 (MQ=255)
aTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCAAATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCa < 1:287053/239‑1 (MQ=255)
gTGAAGGTTACACCCAACGTGCGGGGGACCGACATGCGGAAGTACACGGGTTGCGTATGGCGGGGGAAAAAGCAAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGc < 1:34361/238‑1 (MQ=255)
aCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCCAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCCTCCTGGTCGTACGCCACCGTGCTGTGACGCACTCCTCCCCGCCGGCGTAGCGCGCGGGGTTGCCCCGATGCAAGATCCCAAACCGCCGGGCGCTGGGCGGGGGATTTCCCGTCCGCACCCGGCCGGCCCTGACCTCCGc > 2:292427/1‑238 (MQ=255)
ggggggcgaaaCCCCAAGCCGAAGTACGCGCGTGGCGTATGGCGGGGAAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa < 1:178563/229‑1 (MQ=255)
tGCGGGGGAACCACAGGCGGAAGTACACGCGTTGCGTATGGCGGGTGAAAAGGCCAAAGTGGCACCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa < 1:16329/237‑1 (MQ=255)
gggggTGAAACACATGCCGAAGTACACGCGGTGCGTAGGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTCCGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg < 2:471326/236‑1 (MQ=255)
aGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGCCGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCCCGATGCAAGAGCCTAACCCCCGCGTCCCTGGGCGTGGCCTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCCGATGGTGAGTGAAGcca > 2:50828/1‑238 (MQ=255)
acggggtGCGTAGGGGGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa < 2:370558/233‑1 (MQ=255)
ggctttGCGTATGGCGGGTAAAAATCCAAAAGGTTCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAAt < 2:165296/235‑1 (MQ=255)
gggttaggggggggaaaaaaGCCAAAGGGGGGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAAt < 2:342379/224‑1 (MQ=255)
gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGGACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGGGGACCTTACCCGCTGCAAAAGGCTGGCATTGACCTCAGCCCCGGCCCGAGTGTGAGTGAAGCCGAGCAATTTAATAAAAGCGTtct > 2:322576/1‑238 (MQ=255)
gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTtct > 2:460751/1‑238 (MQ=255)
|
TTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCT > NC_000913/433419‑433870
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
