Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I0 R1 92 125.0 2504412 98.2% 2459332 226.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 433,649 T→G 27.1% G65G (GGT→GGG)  ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913433,6490TG27.1% 124.3 / 20.7 107G65G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (20/51);  new base G (22/8);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 4.58e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 107 total aligned reads displayed.

TTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAC  >  NC_000913/433419‑433885
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGGGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCCAAGAGG‑GGACCg                                                                                                                                                                                                                                        >  2:1064862/1‑238 (MQ=255)
ttggggcaaggagtCGCGGGCCTGGAGGAGTAGGCCGTGCAGGACGATTATTACATGGCGCGGCCGCTAAGGCTGGCGCAAGGAGGACTTTTTCCCACGCATCCCACCCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCAAAATTGGCGGTAAAGG‑TTACCACCAGCGGGGGGGTG‑AACCACATGCCGAAGTACCCGCGTTGCTTATGGC‑GGGTGAAAAAGCAAAAGGTG‑CGACCGc                                                                                                                                                                                                                                       <  2:1048311/226‑1 (MQ=255)
   ggCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGTG‑Cggcaccctc                                                                                                                                                                                                                                    >  2:663623/1‑231 (MQ=255)
        agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGAG‑CGGCCGCCTATGTc                                                                                                                                                                                                                                >  1:274786/1‑238 (MQ=255)
        agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCCAAGGGG‑CGACCGCCTATGTc                                                                                                                                                                                                                                >  1:593853/1‑238 (MQ=255)
        agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGATAAAGCGGGCGCAACGAGGACGTTTTACCACGCATCCCACCCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCCCCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTca                                                                                                                                                                                                                               <  1:931877/239‑1 (MQ=255)
              cgcgcgCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTG‑CGCCCGCCTATTTCACA‑CCCg                                                                                                                                                                                                                        >  1:121837/1‑239 (MQ=255)
              cgcgcgCCGGGAGGACTAAGCCTGGCAGGACGAGTATTACAGGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTGGGGGGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTc                                                                                                                                                                                                                         <  2:702826/238‑1 (MQ=255)
                 gcgcCGGGAGGACTAGGCGGGGCAGGACGAGTATAACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTGGGTGAAGG‑TTACCCCCAACGTGCGGGTG‑ACCCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGaa                                                                                                                                                                                                                      <  2:1034728/238‑1 (MQ=255)
                   gcCTGGAGACCTAAGCCTGGCAGAACGAGTATTACATGGCGCGGCCCTTAAAGCCGGCGCACCGGGAGCTTTTTCCCACGCATCCCAACCCGAATGTCGGGTGCT‑TCATTGCAAAATATGGCGAAATTGTCGGTGAAGG‑TTCCCCCCAACGTGCGGGTG‑AACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAAcc                                                                                                                                                                                                                    <  2:1104771/238‑1 (MQ=255)
                    ccTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAACCAAAAGGG‑CGACCCCCTCTGTCACA‑CTCGCAc                                                                                                                                                                                                                     >  2:1065638/1‑236 (MQ=255)
                      tGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGGGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGT‑TTACCACCAACGTGCGGGTG‑AACCCCATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGt                                                                                                                                                                                                                <  1:834282/239‑1 (MQ=255)
                         cggtcTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTCCCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAg                                                                                                                                                                                                              <  1:1064862/234‑1 (MQ=255)
                             cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCCAAGGGG‑GGAACGCCCATTTCACA‑ACCCAACCCCGTAACcatc                                                                                                                                                                                                         >  2:433047/1‑239 (MQ=255)
                             cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGC‑CGAACGCCTATTTCACA‑ATCGCACCCTGCAGGcatc                                                                                                                                                                                                         >  1:608609/1‑239 (MQ=255)
                                aGCCGTGCAGGAGGAGTATTACATGGGGGGGGCGCTAAAGCGGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTGGGGGGCG‑TCATGGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACAGGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTca                                                                                                                                                                                                                               <  1:877421/215‑1 (MQ=255)
                                    ggggaGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGGGCAACGAGGACGTTTTACCCCGCACCCCACCCCGAAGGTCGGGTGCG‑TCATTGTAAAAGATGGCGAAATTGTCGGTGAAGG‑TTCCCACCAGCGTGCGGGTG‑AACCACAGGCCAAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCg                                                                                                                                                                                                  <  2:299947/235‑1 (MQ=255)
                                     tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAGG‑G‑GGAACCCCCCTGTCACA‑CTCCCACCCTCTAGCCATCATGGTCGt                                                                                                                                                                                                 >  2:436913/1‑239 (MQ=255)
                                     tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGAcc                                                                                                                                                                                                                                         <  1:376495/200‑1 (MQ=255)
                                     tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCCGTAGCCATCATGGTCGt                                                                                                                                                                                                 >  1:155834/1‑239 (MQ=255)
                                       cAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGCTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGG‑CGACCGCCTATGTTCCA‑CTCGAACCCTGTAGCCATCCTGGTCGTAc                                                                                                                                                                                               >  1:268691/1‑239 (MQ=255)
                                          gACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCACGGTCGTAccc                                                                                                                                                                                             >  1:913453/1‑236 (MQ=255)
                                            cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                           <  1:1146780/238‑1 (MQ=255)
                                               gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGG‑GGACCGCCCATGTCACA‑CACGGACCCTGTAGCCATCACGGTCGGTCGcccc                                                                                                                                                                                          >  2:629154/1‑234 (MQ=255)
                                               gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCACAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAGGGG‑CGACCGCCTATTTCACA‑CTCCAAACCTCGAGCCCTGCTTGTCGTACGCCCCCgg                                                                                                                                                                                       >  1:1006588/1‑238 (MQ=255)
                                                 tttcatgggggcgGGCGCAAAAGCTGGGGCAAGGGGGCCGTTTTGCCACGCACCCCACCCCGAATGTGGGGTGCG‑TCATGGTCAAAGAGGGCGAAATGGTCGGTGAAGG‑TTACCCCCAACGTGCGGGTG‑AACCACATGCGGAAGTACACGTGTTGCGTATGGG‑GGGTGAAAAAGCCAAGGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                                      <  2:1116663/228‑1 (MQ=255)
                                                 aTTACATGGGCGGGGGGGTGAAGCGGGGGCAAGAGGGACGTTTTCCCCCGCATCCCACCCCGAGTGTGGGGTGGG‑CAATTGCCAAAGATGGCAAAATTGGCGGTGAAGG‑TTCCCACCCACGGGCGGGAG‑AACCCCATGCCGAAGTCCCCGCGTGGCGTATGGC‑GGGTAAAAACGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                                      <  2:1205605/238‑1 (MQ=255)
                                                 aTTACATGGCGCGGGCGATAAAGCTGGCGCAACGAGGCCGTTTTCCCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCCCCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                                      <  1:188894/238‑1 (MQ=255)
                                                    aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAGACAAAGAGG‑CGACCCGCTTAGTCACA‑CCCGAACCCCGTAGGCCTCATgtctgtatgcacccggcgcgt                                                                                                                                                                                  >  2:443126/1‑219 (MQ=255)
                                                    aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCCAACCCTGTAGCCATCATGGTCGTACGCCACCGGGCtgt                                                                                                                                                                                  >  1:415949/1‑239 (MQ=255)
                                                        ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGG‑CGACCGCCTATGTCCAA‑CTCGAAACCTGTAGCCATCCTGGCCGGACGCCACCGGGCTGTGAc                                                                                                                                                                               >  1:1206729/1‑238 (MQ=255)
                                                        ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAACCATCCTGGGCGGCCGCCACCCGGCTGTGaa                                                                                                                                                                               >  2:747085/1‑237 (MQ=255)
                                                        ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGACAAAACCAAAAGGG‑CGGCCCACTATGTTCCA‑CACGAACCCCTGAGCCATCATGGTCGTAAGCCACCCGGCtgtgtc                                                                                                                                                                               >  2:1235320/1‑236 (MQ=255)
                                                        ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGCTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGAG‑CGCCCGCCCATGTCACACCCCGAACCCT‑TCTCCACCATTGGCCGACGCCCCCGTGCTCTGAc                                                                                                                                                                               >  2:212091/1‑238 (MQ=255)
                                                          cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGc                                                                                                                                                                             >  1:527581/1‑238 (MQ=255)
                                                          cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCCTGCTGTGACGc                                                                                                                                                                             >  1:670925/1‑238 (MQ=255)
                                                          cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGG‑CGACCGCCCATTTCAAA‑CTCGCACCCTGGAACAATCAGGGTCCTACCCCACCCTGCTTTGACGc                                                                                                                                                                             >  1:1075344/1‑238 (MQ=255)
                                                                           gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGAG‑CGACCGCCCATGTTACA‑CTCGAAACCCGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCCCCCATCGCCGCCGGCg                                                                                                                                                            >  2:1181408/1‑238 (MQ=255)
                                                                                 cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgcg                                                                                                                                                     <  1:583290/239‑1 (MQ=255)
                                                                                      aCGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGG‑CGACCCCCTCTTTCCCA‑CCCGACCCCTGTAGCCATCATGGTCGTTCGCCCCCCTGCTGTGACGCCCTCCTCGCCCCGGGCggacgccgcgggg                                                                                                                                                >  2:1179104/1‑227 (MQ=255)
                                                                                           ttACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGG‑CGACCCCCTATGTCACA‑CTCGAACCCTGTACCCCTCATGGGCGTACGCCACCGTGCTCTGGCGCCCCCATCCCGCCCGGCGTCGAGCGCGTGGTTgt                                                                                                                                            >  2:1214398/1‑237 (MQ=255)
                                                                                              ccACGCATCCCAACCCGAATGTCGGGGGCG‑TAATTGACAAAGATGGCGAAATTGTGGGTGAAGG‑TTCCCACCAACGGGCGGGTG‑ACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCg                                                                                                                                        <  2:1227929/239‑1 (MQ=255)
                                                                                                   caccccACCCCGAAGGTCGGG‑GCGTTCATTGTCAAAGATGGGGAAATTGTCGTGGGAGG‑TTCCCACAACCGGGGGGGTG‑AACCACGTGCGAAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAACCCAAGGGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                   <  2:793366/236‑1 (MQ=255)
                                                                                                   cATCCCAACCGGAACGCGGGGTGCG‑TCATTGTAAAAGATGGAGAATTTGT‑GGGGAAGGTTTCCCACCACCGTGCGGGTG‑CACCACAGCCAGAAGTACCCCCGTGGCGTATGGG‑GGGTAAAAAAGCCAAGGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                   <  2:618167/239‑1 (MQ=255)
                                                                                                   cATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGGGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                   <  1:433047/239‑1 (MQ=255)
                                                                                                   cATCCCAACCCGAATGGCGGGGGCG‑TCATTGCAAAAGATGGCAAAATTTTCGGTGAAGG‑TTCCCACCACCGTGCGGGTG‑ACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGC‑CAACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                   <  1:765517/239‑1 (MQ=255)
                                                                                                    cgcccAACCTGAATGCGGGGGGCG‑TCATGGCCAAGAATGGCAAAATTGTCGGGGAAGG‑TTCCCACAGAGTGGCGGTTG‑AACCACATGCCGAATTACACGGTTTGCGTTGGGC‑GGGTGAAAAAGCAAAAGTTG‑CCACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                   <  2:665344/236‑1 (MQ=255)
                                                                                                    acgcaacgcccaaTGTCGGGTGCG‑TCATTGCCAAAGGTGGCGAAATGGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCAGATGCCGAAGTCCACGCGTTGCGTATGGC‑GGTGGAAAAAGCAAAAGTTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                                                   <  2:1221439/227‑1 (MQ=255)
                                                                                                          aCCCGAATGTCGGGGGGG‑TCATTGACAAAGATGGCGAAATTGGCGGTAAAGG‑TTACCCCCAAGGTGGGGGGG‑AACCACAGGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                                                                                                                            <  2:155834/239‑1 (MQ=255)
                                                                                                                gtgggggggGCG‑ACATTGAAAAAGATGGGGAAATGGTCGGGGAAGG‑TTACCA‑CAACGGGGGGGGGAAACCACATGCGGAAGTACACGCGTTGGGTATGGC‑GGGGAAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAccc                                                                                                                       <  2:368442/233‑1 (MQ=255)
                                                                                                                aTGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGCCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGAG‑CGACCGCCCATGTCACA‑ACCCAACCCTGTAGCCAATATGGTCGTACGCCACCGTGCTGTGATGCACCCCTCGCCGCCGGCTTAGCGCCCGTGGCTGCCTCgcatccaaatccctaccc                                                                                                                       >  2:717260/1‑221 (MQ=255)
                                                                                                                 tgtgGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTCCCACCAACGTGGGGGTG‑AACCACATGCCGAAGTACACGCGTTGGGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc                                                                                                                     <  1:239970/239‑1 (MQ=255)
                                                                                                                 tGTCGGGTGCG‑TCATTGTAAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGGGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc                                                                                                                     <  1:924303/239‑1 (MQ=255)
                                                                                                                    gggggggcgcaaTTGTAAACGATGGCGAACGGGCGGGTGAAGG‑TAACCACCACGGGGCGGAGA‑ACCCACATGCCGAATTACCGGGGTGGCGTATGTG‑GGTGGAAAAATCCAAAGGGG‑CTCCCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGc                                                                                                                     <  2:268691/226‑1 (MQ=255)
                                                                                                                    cGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAg                                                                                                                   <  1:1128137/238‑1 (MQ=255)
                                                                                                                      ggTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACg                                                                                                                                                                                              >  1:1072058/1‑161 (MQ=255)
                                                                                                                          cG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGG‑CGACCGCCTATGTCACA‑CTCGGAACCTTTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACCCATCGCCGCTGGCGTAGCGCGCGGGGGTGCCTCGCTGCAAGCTTCTAACCCGCCGGgcgccg                                                                                                            >  2:390297/1‑237 (MQ=255)
                                                                                                                              cATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCCAAGGAG‑CGACCCCCTATGTCACA‑CCCGAACCCTTTAGCCACCATGGTGCTACGCCACCCTGCTGTGACGCCCTCATCGCCCCGGGGGGAGCGCGCCGGGTTGCCTCGCTGCACGCTCCTACCCCCCAGGTCGCTgggg                                                                                                         >  2:498731/1‑238 (MQ=255)
                                                                                                                              aatgtcgaaaGATGGCGAAATGGTCGGTGAAGG‑TTGCCCCAAGCGTGCGGGTG‑AACCCCATGCCGAAGTACAGGCGTGGCGTATGGG‑GGGTGAAAAAGCCAAAGGTG‑CGTCCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                                         <  2:691510/232‑1 (MQ=255)
                                                                                                                                ttGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt                                                                                                       <  1:1181408/239‑1 (MQ=255)
                                                                                                                                          tGGCGAAATTGTCGGGGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACAGGCCGAAGTACACGCGTGGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTa                                                                                               <  1:1077867/237‑1 (MQ=255)
                                                                                                                                           ggCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCg                                                                                            >  1:844844/1‑239 (MQ=255)
                                                                                                                                           ggCGAAATTGCCGGTGAAGG‑TTCCCCCCACCGGGCGGGTG‑AACCACATGCCGAAGTACACGAGTGGCGTATGGC‑GGGTGAAAAGGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCg                                                                                            <  1:1214398/239‑1 (MQ=255)
                                                                                                                                             cGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGGGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGGGGACTTTACCg                                                                                            >  1:1120270/1‑237 (MQ=255)
                                                                                                                                             cGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTCACCCGCAGGGCCCTGGGCGTGGACTTTACCGt                                                                                           >  2:515313/1‑238 (MQ=255)
                                                                                                                                                  ttttCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCCCATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCaa                                                                                     <  1:1240077/236‑1 (MQ=255)
                                                                                                                                                       ggTGAAGG‑TTCCCACCAACGGGCGGGTG‑AACCACATGCGGAAGTACACGCGTGGCGTATGGC‑GGGTGAAAAAGCCAAAGGTC‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAgg                                                                                 <  1:1138139/238‑1 (MQ=255)
                                                                                                                                                        gTGAAGG‑TACCCACCAACGTGCGGGTG‑ACCCACATGCCAAAGTACCCGCGGGGCGTAGGGC‑GGGTAAAAAGGACAAAGGGG‑CGCCCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGc                                                                                <  2:1072992/238‑1 (MQ=255)
                                                                                                                                                              g‑TTACCCCCAACGGGCGGGTA‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCAt                                                                          <  1:37151/238‑1 (MQ=255)
                                                                                                                                                                ttACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGG‑CGACCGCCTATGTCCCA‑CTCGAACCCCGTAGCCATCATGGTCGTACGCCACCGGGCTGTGACGCACTCATCGCCCCTGGCGTAGCGCGCGTGGTTGCCCCGATGCAAGATCCCAACCCGCCGGTCGCCGGGCGGGGGCCTTACCGGCTGCAACAGGCTGGCAttt                                                                        >  2:246726/1‑238 (MQ=255)
                                                                                                                                                                    caccaACGTGCGGGTG‑GCCCACATGCCGAAGTCCACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGTGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                                    <  1:943339/239‑1 (MQ=255)
                                                                                                                                                                    caccaACGTGCGGGTA‑AACCACATGCCGAAGTACACGCTTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                                    <  1:1153589/239‑1 (MQ=255)
                                                                                                                                                                         aCGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGATCCTAACCCGCAGGTCCCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGAAGTCAGc                                                                >  1:186350/1‑238 (MQ=255)
                                                                                                                                                                         aCGGGCGGGTG‑AACCACAGGCCGAAGTCCCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGcc                                                               <  1:1065638/239‑1 (MQ=255)
                                                                                                                                                                          cggccGGGTG‑ATCCACATGCCGAAGTACACGCTTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                              <  1:696134/235‑1 (MQ=255)
                                                                                                                                                                          cGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGTCCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                              <  1:1179104/239‑1 (MQ=255)
                                                                                                                                                                           gggCGGGGG‑AACCACAGCCCAAAGTACACGGGGGGCGTAGGGG‑GGGTAAAAAAGCCAAGGGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                              <  2:19271/236‑1 (MQ=255)
                                                                                                                                                                             gCGGGTG‑AACCACAGGCCGAAGTACACCCGTTGCGTCTGGG‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                            <  1:977010/238‑1 (MQ=255)
                                                                                                                                                                              cGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACgg                                                           >  1:19271/1‑238 (MQ=255)
                                                                                                                                                                              cGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACgg                                                           >  1:879771/1‑238 (MQ=255)
                                                                                                                                                                              cGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGAGAAAAAGCCAAAGGTG‑CGACCGCCTATTTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCCACGGCGCAGCGCGCGTGGTTGCCTCGATGCACGATCCTCAACCGCAGGTCGCTGGGAGGGGGCTTTTCCGTCCGCAAAACGCTGGCCTTGACGTCAGCCACgg                                                           >  2:711755/1‑238 (MQ=255)
                                                                                                                                                                               gggTG‑AACCACATGCCGAATTCCACGCGTTGCGTATGGC‑GGGGTAAAACGCCAAAGGTC‑CGCCCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                                          <  1:717260/238‑1 (MQ=255)
                                                                                                                                                                               gggTG‑AACCACATGCCGAAGTCCCCGCGTTGCGTATGGC‑GGGTAAAAAGCCCAAAGGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                                          <  1:498731/238‑1 (MQ=255)
                                                                                                                                                                                ggTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTCTGTCACA‑CTCGAACCCCGTAGCCATCATGGTCGGACGCCACCCGGCTGTGACGCACTCATCGCCGCCGGCGCAGCGCGCGTGGTTGCCTCGATGCAAGATCCTCACCCGCAGGTCGCTGGGCGTGGGCTTTACCGTCTGCAACAGGCTGGCATTGACGGCAGCCACGGccc                                                        >  2:1240922/1‑238 (MQ=255)
                                                                                                                                                                                      accaccGGCCGAATTCCACGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                    <  2:1120270/238‑1 (MQ=255)
                                                                                                                                                                                          caTCCCGAATTACCCGCTTTGGTTATGGC‑GGGGAAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAg                                               <  1:1187235/239‑1 (MQ=255)
                                                                                                                                                                                           aggCCGAAGTCCACGGGTTGCGTAGGGG‑GGGTAAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGt                                              <  2:415949/237‑1 (MQ=255)
                                                                                                                                                                                            tGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCCATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCCCCGCCGGCGTAGCGCGCGCGGTTGCCTCGCTGGCAGATCCCAACCCGCAGGGCGCTGGGGGTGGGCTTTACCGCCTGCAACAGGCTGGCGTTGACGGCCGCCACGGCCtgatgtggaggg                                             >  2:133723/1‑232 (MQ=255)
                                                                                                                                                                                                 aaGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGTGG‑CGGCCGCCCATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGGACGCCACCGTGCGGTGACGCCCTCAACCCCGCTGGCGGAGCGCGCATGGTTGCCTCCATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGGCTGTACCGGCTGCCACCAGCTGGCATTGACGTCCGCCACGGGCTGGTGATGAGTGAAGc                                         >  2:234673/1‑238 (MQ=255)
                                                                                                                                                                                                  aGTACACGGGGTGCGTATGGG‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                       <  1:1093863/239‑1 (MQ=255)
                                                                                                                                                                                                  aGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                       <  1:747085/239‑1 (MQ=255)
                                                                                                                                                                                                     acacGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                     <  1:246726/238‑1 (MQ=255)
                                                                                                                                                                                                         gcgTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGCGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGGGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAAtt                                >  1:1064628/1‑239 (MQ=255)
                                                                                                                                                                                                            ttGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGGGCTGGGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGGCGTCAGCCCCGGCCTGATGATGGGTGGAGCCGAGCAATTGaa                             >  1:1159611/1‑239 (MQ=255)
                                                                                                                                                                                                               cGTAGGGT‑GGGTGAAAAAG‑CAAAGGTGCCGCCCGCCTATGGCCCC‑CTCGAACCCTGTAGCCAGCATGGTCGTACGCCACCGTGCTGGGACGCACTCATCGCCGCTGGCGTAGCGTGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATa                           <  2:766583/238‑1 (MQ=255)
                                                                                                                                                                                                                         ggTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTtc                  <  1:983314/238‑1 (MQ=255)
                                                                                                                                                                                                                         ggTAAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTtc                  <  1:1043018/238‑1 (MQ=255)
                                                                                                                                                                                                                            gAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCa               <  1:436913/238‑1 (MQ=255)
                                                                                                                                                                                                                                       aGGTG‑CGCCCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCa   <  2:527581/239‑1 (MQ=255)
                                                                                                                                                                                                                                         gTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAc  <  1:212091/238‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
TTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCG‑TCATTGTCAAAGATGGCGAAATTGTCGGTGAAGG‑TTACCACCAACGTGCGGGTG‑AACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTG‑CGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCAC  >  NC_000913/433419‑433885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A