Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F27 I0 R1 120 139.6 2835800 98.5% 2793263 226.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 433,649 T→G 29.6% G65G (GGT→GGG)  ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913433,6490TG29.6% 121.5 / 26.0 122G65G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (24/54);  new base G (27/10);  total (57/65)
Fisher's exact test for biased strand distribution p-value = 4.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 122 total aligned reads displayed.

AGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCA  >  NC_000913/433415‑433884
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
aGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGa                                                                                                                                                                                                                                           <  1:788804/239‑1 (MQ=255)
aGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCGGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGa                                                                                                                                                                                                                                           <  2:146225/239‑1 (MQ=255)
   aTTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGAc                                                                                                                                                                                                                                          <  2:582264/237‑1 (MQ=255)
   aTTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGCGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGAGCAAcc                                                                                                                                                                                                                                         >  1:1223855/1‑238 (MQ=255)
    tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCCCATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGGCCAAGGGGCggacc                                                                                                                                                                                                                                        >  2:593068/1‑234 (MQ=255)
     tgggCGAAGAGACCGCGCGCTTGGAGGACTAACCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCGGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGcc                                                                                                                                                                                                                                      <  2:325383/237‑1 (MQ=255)
      tGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCCAAGGTGGGACcccc                                                                                                                                                                                                                                      >  1:1051676/1‑238 (MQ=255)
       ggCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGAGCGgccgccca                                                                                                                                                                                                                                    >  1:1106538/1‑237 (MQ=255)
           aaGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGt                                                                                                                                                                                                                                 <  2:1294200/238‑1 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCGACCGCCCATGTc                                                                                                                                                                                                                                >  2:1329278/1‑238 (MQ=255)
            agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCCAAGATGCGCACGCCCTTGTc                                                                                                                                                                                                                                >  2:318916/1‑238 (MQ=255)
            agaagtCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGGGTATTACAGGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCACCCCAC‑CCCGAATGTCGGGTGCGGCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                               <  2:966695/234‑1 (MQ=255)
                   gggggcCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTCCCACGCACCCCAC‑CCCGAAGGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                                        <  1:1187934/235‑1 (MQ=255)
                   gcgcgcCTGGAGGACTAAGCCGTGGAGGACGAGTATTACAGGGCGCGGGCGCTAAAGCTGGGGGAACGAGGCCGTTTTCCGCCGCATCCCAG‑CCCGAATGTCGGGTGCGTCATTGTAAAATATGGCGAAATTCGCGGGGAAGGTTACCACCAAGGGGGGGGTGAACCACATGCCGAAGTCCCCGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                                        <  2:1401933/239‑1 (MQ=255)
                   gcgcgcCTGGAGGACGAAGCCGGGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGTTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                                        <  1:593068/239‑1 (MQ=255)
                     gcgcCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTCCGACCGCCTATGTCACACTCGaa                                                                                                                                                                                                                       <  2:331923/238‑1 (MQ=255)
                         ggggaggaCTAAGCCGTGCAGGACGATTATTACATGCCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTAAAGGTTACCACCACCGTGCGGGTGAACCACAGGCCGAAGTACACGCTTTGCGTATGGC‑GGTTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTg                                                                                                                                                                                                                  <  2:1303463/237‑1 (MQ=255)
                            gaggaCTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAC‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTa                                                                                                                                                                                                                <  1:41502/238‑1 (MQ=255)
                            gaacgcgaggCCGGGCAGGACGAGTTTTACGTGGCGCGGGCGGTAAAGCGGGCGCACCGAGGGTGTTTTACCACGCATCCCAC‑CCCAAATGTCGGGTGCTTCATTGTCAAAGATGGCGAAATTGGCGGGGAAGTTTACCACCACCGTGCGGGTGAACCACAGGCGGAAGTACGCGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTa                                                                                                                                                                                                                <  2:1223855/229‑1 (MQ=255)
                                 cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAGGTGCGACCGCCTATGTCCAACTCCAACCCCGTAGCcatc                                                                                                                                                                                                          >  2:1375951/1‑239 (MQ=255)
                                   aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGAGCGGCCCCCCATGTCACACTCGAAccccctaagcca                                                                                                                                                                                                           >  2:821513/1‑227 (MQ=255)
                                          gCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGCGGCCGCCTCTGTCCAACTCCAACCCCGGCGCCATCATGGTCgg                                                                                                                                                                                                  >  2:1103631/1‑237 (MQ=255)
                                            aagACGAGTTTTACCTGGCGGGGGCGCAAAAGCTGGCGAAACGAGGACGTTTTCCCACGCACCCCAA‑CCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑Cg                                                                                                                                                                                              <  2:1108952/237‑1 (MQ=255)
                                                ggAGTATTACAGGGTGGTGGCGTCAAGGTTGGCGCAACGAGGCTGTTTT‑CCCCGCCACCCAACCCCGGATGTCGGGTGCGTCATTGTAAAGGATGGCGAAATTTGGGGAGAAGGTTCCCCCCAACGTGCGGGGGACCCACTGGCGGAAGTCCACGCTTTGTGTATGGC‑GGGTAAAAAACCCAAGGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCa                                                                                                                                                                                           <  2:343039/237‑1 (MQ=255)
                                                cgccgagtactgtgcgaGGGCGCTAAAGCGGGCGCAAGGTGGGGGGGTTCCCACGCTTCCAAA‑CCCGAATGTGGGGGGCCACATGGTCAGAGGTGGGGAACTAGCTGTTGAGGTTTACACCCAACGGGCGGGTGAACCACAGGCCGAACAACAGACGTTGCGCAGGGC‑GGGTAAAAAAGCCAAGGGAGGAACCGCCTATGTCACACTCGAACCCTGTAGCCGTCATGGTGGTA‑CGCCa                                                                                                                                                                                           <  2:226881/225‑1 (MQ=255)
                                                cGAGTATTACAGGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCCCGCATCCCAC‑CCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCa                                                                                                                                                                                           <  1:1375951/238‑1 (MQ=255)
                                                   gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGTA‑CGCCCCCGt                                                                                                                                                                                       >  1:881402/1‑239 (MQ=255)
                                                   gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGGCCGCCCATGTCACCCTCGAACCCTGTAGCCATCATGGGCGTA‑CGCCACCGt                                                                                                                                                                                       >  1:624102/1‑239 (MQ=255)
                                                   gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGAGCGCCCATGTCACACTCGAACCCTGCAGCCCTCATGGTCGTA‑CGCCCCCGt                                                                                                                                                                                       >  1:453169/1‑239 (MQ=255)
                                                          aTGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAAGGGGGACCCCCCATGTGCAACCCGAAACCTGCAGCCAGCATGGTGGGA‑AGCCCCCCTGCtgtg                                                                                                                                                                                 >  2:728967/1‑238 (MQ=255)
                                                            ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACCCTCGAACCCTGTAGCCATCCTGGTCGGC‑CGCCACCGGGCTGTGAc                                                                                                                                                                               >  1:122648/1‑238 (MQ=255)
                                                            ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGAc                                                                                                                                                                               >  1:197833/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGgcgc                                                                                                                                                                             >  1:103791/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGCGGGA‑AGCCCCCGTGCTGGGACGc                                                                                                                                                                             >  1:745268/1‑238 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGCACACGCGTTGCGTATGGC‑GGGAGAAAAAAACAAAAGGGCGACCCCCCATGTCCTACCCGAACCCCTCCGTCATCCTGGTGGTA‑CGCcccccctctgttgccc                                                                                                                                                                             >  2:762065/1‑226 (MQ=255)
                                                              cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTAACACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGACAAAGGCGGGAACGGCTATGTCACACCCCAAACCTGGAGACATTTTTGTCGTACCGCCAcccgctgttgaca                                                                                                                                                                              >  2:1417009/1‑227 (MQ=255)
                                                               gcgGGCGCAAAAGGTGCGGCAAGGGGGGCGTTGTACCACGCATCCCAC‑CCCAAAGTTCGGGTGGTTAATTGTAAAGAAGGGGGAAATTTGGGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTCCCCGCTTGGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCa                                                                                                                                                                            <  2:1170627/238‑1 (MQ=255)
                                                                cgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCAc                                                                                                                                                                           >  1:446909/1‑238 (MQ=255)
                                                                  ggCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACt                                                                                                                                                                          >  1:975466/1‑237 (MQ=255)
                                                                   gcgcTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTAACACCAACGTGCGGGTGAACCACATGCCGAAGCACACGCGTTGCGTATGGC‑GGGGGAAAAAAAGACAAGTGCGACCGCCTCTGTCACACCCGAACCCCCTAGCGATAATGGTCAGA‑AGCCACCGTGCtgtgtgccacgcc                                                                                                                                                                        >  2:854413/1‑229 (MQ=255)
                                                                        aaggggggCGCAACGAGGACGTTTTACCACGCATCCAAC‑CCCGAATGTGGGGGGCGTCATGGGAAAAGAGGGCGAAAGGGGCGGTGAAGGTTACCACCAACGGGCGGGGGAACCACATGCCGAAGTCCCCGCGTTGCGTAGGGC‑GGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATcgccg                                                                                                                                                                  <  2:1051676/233‑1 (MQ=255)
                                                                               gcgcAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGTAAAAGCCCAAGGAGCGAACGCCCATGTCACACTCGGACCCTGTAGCCCTCCTGGGCGTA‑CGGCACCGGGCTGTGACGCAATCACCGCCCCCGGCGt                                                                                                                                                           >  2:612475/1‑239 (MQ=255)
                                                                               gcgcAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGt                                                                                                                                                           >  1:1005539/1‑239 (MQ=255)
                                                                                cgcAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTa                                                                                                                                                          >  1:165588/1‑239 (MQ=255)
                                                                                                  ccACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCg                                                                                                                                       <  1:1049108/239‑1 (MQ=255)
                                                                                                  ccACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACAAACGTGCGGGTGAACCACAGGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCg                                                                                                                                       <  1:467429/239‑1 (MQ=255)
                                                                                                   cACGCATCCCAA‑CCCTAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGa                                                                                                                                      <  1:817586/239‑1 (MQ=255)
                                                                                                      gCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCCGGCGTAGCGCGCG‑TGGTTGCCTCGATGc                                                                                                                                   >  1:1340022/1‑239 (MQ=255)
                                                                                                      gCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGGGGGACCGCCCATGTCACACCCCAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGGGACGCACTCATCCCCCCTCGCGCTGCGCGCG‑TGGGTGCCTCGATg                                                                                                                                    >  2:845334/1‑238 (MQ=255)
                                                                                                       cttCCCAA‑CCCGAATGTCGGGTGCGAAATTGTAAAAGATGGCGAAATTGGCGGTGAAGGTTACCACAAACGGGCGGTTGAACCACATGCCGAAGTACACGCGTTGCTTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCa                                                                                                                                  <  2:59354/237‑1 (MQ=255)
                                                                                                       cATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCa                                                                                                                                  <  2:446909/239‑1 (MQ=255)
                                                                                                        cttcccaccccaaagtgcGGGGGGGTCATTGTCAAAGAGGGCGAATTTGTCGGTGAAGTTTCCCACCAACGTGCGGGAGACCCACATGCCGAAGTCCCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCa                                                                                                                                  <  2:97973/222‑1 (MQ=255)
                                                                                                        cacccAACCCCGAATGCGGGGTGCGTCATTGTCAAAGAGGGCAAAATTGTCGGGGAGGGTTCCCCCCAACGTGCGGGTAAACCACATGCCGAAGTCCCCGGGTGGCGTTTGGG‑GGGTAAAAAAGCAAAGGGTGCTACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCa                                                                                                                                  <  2:1044663/237‑1 (MQ=255)
                                                                                                        aTCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGGCGGTGAAGTTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                                        <  1:1303965/154‑1 (MQ=255)
                                                                                                          cccAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCCCCGTGCTGTGACGCACTCATCCCCCCTGGCGGAGCGCGCG‑TGGTTGCCTCGATGCAAGa                                                                                                                               >  1:905886/1‑239 (MQ=255)
                                                                                                          cccAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGGGCGACCGCCTATGTCACACTCGAAACCTGTAGCCATCATGGTCGGA‑CGCCACCGTGCTGTGACGCCCCCATCGCCCCCGGCGGAgccgggctgggtggctcgtggcaaa                                                                                                                                 >  2:608376/1‑215 (MQ=255)
                                                                                                             caccccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCCCCACCGTGCGGGTGAACCACATGCGGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATc                                                                                                                             <  1:318916/236‑1 (MQ=255)
                                                                                                                   gAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCGACCGCCCATGTCACACTCGAACCCTGTAGc                                                                                                                                                                                                              >  2:1303965/1‑154 (MQ=255)
                                                                                                                     aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAACAGCAAAAGG‑GGGAACGCCTATGTCACCCTCGAACCCCGTAGCCATCATGGGTGTA‑CGCCACCGGGGTCTGCGGGACACCTCGCCGCCGGGGTCGCGCGCGCGGGTTGcccgcgggcaactcgcaaacc                                                                                                                       >  2:852540/1‑219 (MQ=255)
                                                                                                                     aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCCATGTCACACTCGAACCCCGGAGCCATCATGGTCGTA‑CGCCCCCGCGCTGGGGCGCACCCACCGCCGCTGGCGGAGGGCGCG‑GGGTTGCCCCCAGGCCAGATCCTAcccc                                                                                                                      >  2:680455/1‑238 (MQ=255)
                                                                                                                        tggggTGCGTCATTGGCAAGGATGGCGAAATTGGAGGGGAAGGTTACCCCCAACGGGCGGGTGAACCACATGCCGAAGTCCACGCGTGGCGTATGGC‑GGGTAAAAAGGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGc                                                                                                                    <  2:436762/235‑1 (MQ=255)
                                                                                                                         cGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAgg                                                                                                                 >  1:773712/1‑239 (MQ=255)
                                                                                                                          gggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGTGCGACCGCCTATTTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCCCCGTGCTGTGACGCACCCATCCCCGCTGGCGTAGCGGGCG‑GGGGTGCCTCGATGCCAGATCCTAACCCCCAgg                                                                                                                 >  2:1111644/1‑238 (MQ=255)
                                                                                                                           ggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCCAAAGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCCTCATTGTCGTA‑CGCCCCCGTGCTGTGACGCACTCATCGCCGCCGGCGTAGAGCGCG‑TGGTTGCCTCCATGCAAGAAACTAACCCGCCggg                                                                                                                >  2:1363792/1‑237 (MQ=255)
                                                                                                                               cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAAGTGCGACCGCCTATTTCACACTCGAACCCCGTAGCCATCATTGTCGGA‑CGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTCGCGCGCG‑TGGTTGCCTCGGTGCAAGATCCTAACCCGCAGGTCGCTg                                                                                                           >  2:601229/1‑239 (MQ=255)
                                                                                                                                    ttGTCAAGGATGGCGAAATTGTCGGGAAGGGTTCCCCCCAACGGGCGGGTAAACCACATGCCGAAGTACACGGGTGGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt                                                                                                      <  2:1050016/239‑1 (MQ=255)
                                                                                                                                    ttGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGt                                                                                                      <  2:514462/239‑1 (MQ=255)
                                                                                                                                     tGTCAAGGATGGCAAAATTGTCGTTGAAGGTTACCACCAAGGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGGAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTg                                                                                                     <  2:364922/239‑1 (MQ=255)
                                                                                                                                         aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGaa                                                                                                                                                                                                                       >  2:96824/1‑123 (MQ=255)
                                                                                                                                         aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGaa                                                                                                                                                                                                                       <  1:96824/123‑1 (MQ=255)
                                                                                                                                               ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGTGCGACCGCCTATTTCACACTCGAACCCTGTAGCCATCCTGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑GGGTTGGCTCGATGCCAGATCCTCACCCCCACGGCGCCggggggggcgttttccg                                                                                           >  2:376396/1‑225 (MQ=255)
                                                                                                                                                 cGAAATTGTGGGTGAGGGTTACCACCAACGTGCGGGTAAACCACATGCAGAAGTGCGCGCGTTGCGTATGGC‑GGGTAAAAAGCCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGt                                                                                          <  1:1029632/238‑1 (MQ=255)
                                                                                                                                                 cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTTGGCGTGGACTTTACCg                                                                                           >  1:836340/1‑237 (MQ=255)
                                                                                                                                                 cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGGTTCCTCGATGCAAGATCCTAACCCCCAGGACGCTGGGCGGGGGCTTTACCg                                                                                           >  2:1348900/1‑237 (MQ=255)
                                                                                                                                                 cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAGAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCCCCGTGCTGTGACGCCCTCCTCTCCGCTGGCGTAGCGCGCG‑GGGTTGCCTCGATGCAAGACCCTAACCCGCAGGTCGCTGCGCGCGGACTCTACCg                                                                                           >  2:1168845/1‑237 (MQ=255)
                                                                                                                                                            gggagggTTACCACCAGCGGGGGGGTGAACCACATGCCGAGTTCCCCGCGTGGCGAATGGC‑GGGTAAAAAAGCCAAAGTGGCGCCCGCCTATGTCACGCTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGc                                                                               <  2:1280171/233‑1 (MQ=255)
                                                                                                                                                                             cgggCGGGTGAACCACAGGCCGAAGTCCACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                             <  1:1111644/236‑1 (MQ=255)
                                                                                                                                                                             cGTGCGGGTGACCCAACGGCCGAATTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                             <  1:1329278/239‑1 (MQ=255)
                                                                                                                                                                             cGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGGGCGCG‑GGGGTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGcc                                                              >  2:532947/1‑238 (MQ=255)
                                                                                                                                                                                gggggTAACCCATATGCCGAAGTGCACGCGTGCTGTAGGGC‑GGTAGAAAAAGCAAAATGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                           <  2:836548/236‑1 (MQ=255)
                                                                                                                                                                                 cGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGAAGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGGCTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACgg                                                          >  1:538172/1‑238 (MQ=255)
                                                                                                                                                                                     tGACCCACATGCCAAAGTACACGCGTTGCGTTTGGC‑GGGTGAAAAAGCAAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtg                                                      <  1:291709/238‑1 (MQ=255)
                                                                                                                                                                                       aaCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                   >  1:114185/1‑239 (MQ=255)
                                                                                                                                                                                       aaCCACAGGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                   <  1:608376/239‑1 (MQ=255)
                                                                                                                                                                                        ccaccaTGCGAAAGTACCCGCTTTGCGAATGGC‑GGGTAAAAACGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                   <  1:302243/234‑1 (MQ=255)
                                                                                                                                                                                        aCCACAGGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatg                                                   <  1:174508/238‑1 (MQ=255)
                                                                                                                                                                                                 cgaggcacccgcgTTGCGTATGGG‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGCGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAg                                         <  2:475613/230‑1 (MQ=255)
                                                                                                                                                                                                    aGTACACGTGTTGCTTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAAGCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCACCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                                      <  1:1162401/239‑1 (MQ=255)
                                                                                                                                                                                                       acacGCTTGGCGTAGGGC‑GTGTGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                    <  2:512286/238‑1 (MQ=255)
                                                                                                                                                                                                       acacGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                                    <  1:304094/238‑1 (MQ=255)
                                                                                                                                                                                                         cggggtggcgttgggC‑GGGTAAAAAAGCAAAGGGGCCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa                                 <  2:881402/226‑1 (MQ=255)
                                                                                                                                                                                                         acccgTTCCTTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCaa                                 <  1:1363792/236‑1 (MQ=255)
                                                                                                                                                                                                             gTTGCGTATGGC‑GGGTGAAAAAGCCCAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGGGGACTTTACCGTCTGCAACAGGCCGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGa                             >  1:1209063/1‑239 (MQ=255)
                                                                                                                                                                                                             gTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATTGTCGTA‑CGCCACCCTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TTGGTGCCTCCATGCAAGATCCTCACCCGCCCGTCGCTGGGCGGGGACTTTTCCGTCGCCAACAGGCCGCGCTTGGCGTCCGCCCCGGTCTTATGACTGTTGAAGCCGAGCaacttg                             >  2:1297056/1‑235 (MQ=255)
                                                                                                                                                                                                                gCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATa                          <  1:549486/239‑1 (MQ=255)
                                                                                                                                                                                                                     tGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGc                     <  1:1103631/239‑1 (MQ=255)
                                                                                                                                                                                                                     tGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAg                       >  1:221502/1‑237 (MQ=255)
                                                                                                                                                                                                                            gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTtct                >  1:334151/1‑238 (MQ=255)
                                                                                                                                                                                                                            gTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGACATCATGGTCGTA‑CGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCGCTGGGCGTGGGCTTTACCGTCTGCAACAGGCTGGCCATGGCGGTAGCCCCGGCCTGGGGGTGAGTGAAGACGGGCAATTTAATAAAGGCTTtct                >  2:1364835/1‑238 (MQ=255)
                                                                                                                                                                                                                                        aaGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATgcgc   <  2:1398429/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
AGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAA‑CCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTA‑CGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCG‑TGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAGCGGATGCGCA  >  NC_000913/433415‑433884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A