Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F9 I0 R1 103 124.7 2465218 97.5% 2403587 230.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 433,649 T→G 16.8% G65G (GGT→GGG)  ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913433,6490TG16.8% 146.9 / 4.9 96G65G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (23/50);  new base G (14/4);  total (42/54)
Fisher's exact test for biased strand distribution p-value = 8.52e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

AAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAG  >  NC_000913/433414‑433874
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   
aaGAATTTGGGGAAGAAATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGGTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTAATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑CCCAACGGGCGGGTGAACCACATGCCGAAGTCCACGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGTGCg                                                                                                                                                                                                                                  <  1:713013/239‑1 (MQ=255)
 aaaaTTTGGCGAAGAGACCGCGCCGCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGGGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCAAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGa                                                                                                                                                                                                                                 <  2:1127808/237‑1 (MQ=255)
    aTTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGAc                                                                                                                                                                                                                                <  2:471289/237‑1 (MQ=255)
    aTTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGAGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGTAAAAGACCAAAGAGCGAcc                                                                                                                                                                                                                               >  1:1127808/1‑238 (MQ=255)
     tttGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGGGCGAcgc                                                                                                                                                                                                                              >  2:885391/1‑236 (MQ=255)
        ggCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAAGTGGGACCGCCTa                                                                                                                                                                                                                          >  2:1041943/1‑239 (MQ=255)
             agagagCGGGCGCCTGGAGGAATAACCCGTGGAGGACGAGTATTCCATGGCGCGGGCGCTAAAGCGGGCGAAACGAGGACGTTTTCCCACGCATCCCACCCCGAATGTGGGGGGCGTCATTGTCAAAGATGGCGAAATTGTGGGTGAAGGTTCCC‑ACCACCGTGCGGGTGACCCACATGCCGAAGTACCCGCGTGGCGTATGGCGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                     <  1:1031435/239‑1 (MQ=255)
             agagATCGCGCGCCTGGAGGTCTAATCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                     <  2:974713/239‑1 (MQ=255)
             agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAAGGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                                                                                                                     <  2:894708/239‑1 (MQ=255)
             agagATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCACAGGAGCGACCGCCCATGTc                                                                                                                                                                                                                      >  2:770386/1‑238 (MQ=255)
                   cgcgcgCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCg                                                                                                                                                                                                               <  2:1056028/239‑1 (MQ=255)
                   cgcgcgCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGCCAAAGGGGCGACAGCCCATGTCCCACTCg                                                                                                                                                                                                               >  1:804964/1‑239 (MQ=255)
                    gcgcgcCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                                                                                                                              <  2:1189752/239‑1 (MQ=255)
                     gcggcCGGGAGAACTAGCCCGTGCAGGACGTGTATTCCATGGCGCGGGCGCTAAACCTGGCGCACCGAGGACGTTTTCCCACCCATCCCACCCCGAATGTCGGGGGCGTCATTGTCAAAGATGGAGAAATTGTCGGTGAAGGTTACC‑CCCAGCGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGGGGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGaa                                                                                                                                                                                                             <  1:940454/236‑1 (MQ=255)
                            ggaggcCAAAGCCTTGAGGAACAAGTATAGAATGGGGCGGGGCCTAAGGCTGGGCCAACGGGGCCGTTTTACGACCAATCCCACCCCCATGGTCGGGTGGCACCTGGGAAAAGAGGCAGAAATTGTGGGTGAAGGTGACC‑TCAAAGGTGCGGGTGACCCACAGGCCGGGGTACCCGCTTTGGGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTa                                                                                                                                                                                                      <  1:992520/239‑1 (MQ=255)
                                  cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcctc                                                                                                                                                                                                >  2:206054/1‑239 (MQ=255)
                                  cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatc                                                                                                                                                                                                <  2:402638/239‑1 (MQ=255)
                                  cTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCACAGGTGGGACCGCCTATGTCAGACTCCGACCCTGTAGCcatc                                                                                                                                                                                                >  2:291255/1‑239 (MQ=255)
                                    aaGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCCAAGGGGCGAACGCCCATGTCACACCCCAACCCTGTAGGcatcct                                                                                                                                                                                              >  1:886843/1‑237 (MQ=255)
                                            cAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAc                                                                                                                                                                                      >  2:404799/1‑239 (MQ=255)
                                               gACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAcac                                                                                                                                                                                    >  2:855316/1‑236 (MQ=255)
                                                actattATTCCATGGAGTGGGCGCTAAAGCGCGGCCTACGAGGTCGCTTTACCACGCATCCCAACACGAATGTCGGGTGCGGCATGGTAAAAGAGGGCGATATTGTCGGTAAAGGTTACC‑GCCAACGTGGGGGTGAACCACATGCGGAATTACACGGGTTGCGTAGGGGGGGAAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGATCCCTGTAGCCATCATGGTCGTACGc                                                                                                                                                                                    <  1:655081/232‑1 (MQ=255)
                                                aCGAGTATTACATGGCGCGGGCGTTAAAGCTGGCGCAACGAGGACTTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGcc                                                                                                                                                                                   <  2:261993/238‑1 (MQ=255)
                                                 cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGCACCCTGTAGCCATCATGGTCGGACGCCa                                                                                                                                                                                  >  2:755357/1‑238 (MQ=255)
                                                 cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                  >  2:545603/1‑238 (MQ=255)
                                                 cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGCGAGAAAGCCCAAAGTGCGACCGCCCACTTCCTGCTCGCCCCCCGTAGCCCCCACGGTCGTACGCCa                                                                                                                                                                                  >  1:1198881/1‑238 (MQ=255)
                                                 cGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGGGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                  <  2:262297/238‑1 (MQ=255)
                                                    gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGt                                                                                                                                                                              >  2:1077102/1‑239 (MQ=255)
                                                         aCATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGGCTGGTTCAAAAGGCAAAGCTGCGACCGCCTAGCTGACACTCGCACTCTCTAGCCACCATTGTCGTACCCCCCCCGGCtg                                                                                                                                                                          >  1:1165814/1‑238 (MQ=255)
                                                             ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCCTCATGGTCGTACGCCACCGTGCTGTGAc                                                                                                                                                                      >  2:732965/1‑238 (MQ=255)
                                                             ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGAc                                                                                                                                                                      >  2:1043038/1‑238 (MQ=255)
                                                              gcgcGGGCGTTAAAGCGGGCGCAACGAGGACGTTTTACCGCGCATCCCACCCCGAAGGTCGGGTGCGTCATTGTAAAAGATGGCGAAATTGTGGGTGAAGGTTACC‑ACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGAc                                                                                                                                                                      <  1:1081997/237‑1 (MQ=255)
                                                               cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGGAAAAACCAAAGGCGCGACCGCCTATGTCACACCCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGAcac                                                                                                                                                                    >  2:797575/1‑236 (MQ=255)
                                                               cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGACCAAGGTGCGACCGCCTATTTCTCACTCGAACCCCGTAGCCATCATGGTCGCACGCCCCCGTGCTGTGACGc                                                                                                                                                                    >  2:211508/1‑238 (MQ=255)
                                                               cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCAAAGGCGCGACCCCCTCAGTCACACATGAAACCCGTAGCCCTCACTGTCGTACGCCCCCCTCCTGTGActc                                                                                                                                                                    >  1:132505/1‑236 (MQ=255)
                                                                    gcgcTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCCGACCCTGTAGCCATCATGGTCGTACGCCACCCTGCTGTGACGCACTCa                                                                                                                                                               >  2:1081997/1‑238 (MQ=255)
                                                                      gcTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                                                                                                                             <  2:628630/238‑1 (MQ=255)
                                                                         aaaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcg‑ccg                                                                                                                                                        <  2:923779/239‑1 (MQ=255)
                                                                                      cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAgcgcg                                                                                                                                           <  2:124993/239‑1 (MQ=255)
                                                                                            cGTTTTCCCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTa                                                                                                                                                                                                      <  2:471376/175‑1 (MQ=255)
                                                                                                 ccccACGCCACCCACCCCGAAGGTCGGGTGCGGCATGTTCAAAGATGGCCAAATGGTCGGGGAAGGTTACC‑ACCAACGGGGGGTTGAACCGCATGCCGAAGGCCACGCGTTGCGTATGGCGGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCt                                                                                                                               <  1:409268/237‑1 (MQ=255)
                                                                                                      ctcctCCCACCCCGAATGGGGGGGCTGCCATTGGCAAAGAGGGGGAATTTGCCGGTGAA‑GTTACCAACCAACGTGCGGGTGACCCACAGGCCGAAGTACCCGCGTTGGGTATGGCGGTTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGGGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATg                                                                                                                          <  1:789593/235‑1 (MQ=255)
                                                                                                      cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGGAAAAACCAAAGGAGAGACCCCCTATGTCACACTCGAACCCTGTAGCCATCATgg                                                                                                                                                                                            >  1:471376/1‑175 (MQ=255)
                                                                                                       gCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCCCCCCAGTCACACTCCAACCCTGTAGCCATCCTGGTCGTACGCCACCGTGCTGTGACGCACTCATCC‑CCGCTGGCGGAGCGCGCCGTGG‑TGCCTCGATg                                                                                                                          >  1:338079/1‑238 (MQ=255)
                                                                                                        caccccAACCAGAATGTTGGGTCCGTCGTGTGAAAAGGTGGCGAAATTGGCGGTGAAGGTTACC‑ACCAACGTGCGTGAGAACCACGTGCCGAAGGACGCGCTTGGCGTAGGGGGGGTAAAAAAGCAAAAGGTGGGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCa                                                                                                                        <  1:1195462/236‑1 (MQ=255)
                                                                                                        cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGCCAAAAGGGCGACCGCCTATGTCACAATCGAACCCTGTAGCCAGCATGGGCGTAAGCCACCGTGCTGTGACGCACTCATCA‑CCCCTGGCGGAGCGCG‑CGTGGTTGCCTCGGTGCa                                                                                                                        >  1:490566/1‑239 (MQ=255)
                                                                                                         aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCC‑CCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCa                                                                                                                        <  2:904323/238‑1 (MQ=255)
                                                                                                         aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCa                                                                                                                        <  2:762369/238‑1 (MQ=255)
                                                                                                           cccAACCGGAAGGGCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAAGGGGGGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                                                                                                                  <  1:513455/180‑1 (MQ=255)
                                                                                                                  cGAATGTCGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAAc                                                                                                              <  1:1077102/239‑1 (MQ=255)
                                                                                                                     aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCtgtg                                                                                                                                                                        >  2:513455/1‑180 (MQ=255)
                                                                                                                      tGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGGGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGc                                                                                                          <  2:554311/239‑1 (MQ=255)
                                                                                                                              gCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACAGGCCGAAGTACACGCTTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc                                                                                                   <  2:115155/238‑1 (MQ=255)
                                                                                                                                  cATTGTCAAAAAGCGCGAAATGGTCGTTGAAGGTTACC‑CCCAGCGTGGGGGGGAACCACATCCCTAAGTACCCGTGTGGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                              <  1:853259/239‑1 (MQ=255)
                                                                                                                                  cATTGGCAAAGTGGGCGAATTTGGTGGTGAAGGTTCCC‑ACCAACGTGGGGGTAACCCACATGCCGAAGTCCCCGCGTTGCTTATGGCGGGTGAAAAAGCCAAAGGGCCAACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc                                                                                              <  1:324917/239‑1 (MQ=255)
                                                                                                                                             gtggaaaatgtgtgtatGAAGGTACCC‑ACCGGCTCGCGGGTAATGCACCCGCCGATGTACACGCGTGGCGTATGGCGGGCGAAACATCCAAGGGTCCCCCCGCTTATGTCTCACTCGAACCCTGTAGCCATGATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTATCCCGCAGGTCGCTGGGCGTGGACTTTa                                                                                    <  1:885391/222‑1 (MQ=255)
                                                                                                                                              tGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCCCCGGCGTAGCGCG‑CGGGGGTGCCTCGATGCAAGCTCCTAACCCGCCGGGCGCTGGGCGCGGGCGTTAcc                                                                                  >  1:647735/1‑239 (MQ=255)
                                                                                                                                                 cGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGAACCTAACCCGCAGGGCGCTGGGCGTGGGCTTTACCg                                                                                 >  2:387008/1‑237 (MQ=255)
                                                                                                                                                 cGAAATTGGCGGGGAAGTTTACC‑ACCAGGGTGGGGGTGAACCACATGCCGAAGGCCCCGTGTTGCGTATGGGGGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGt                                                                                <  1:1122330/238‑1 (MQ=255)
                                                                                                                                                           ggTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAgg                                                                      <  2:132505/238‑1 (MQ=255)
                                                                                                                                                            gTGAGGGTTCCC‑CCCAACGGGGGGGGGAACCACATGCGGAAGTACACGGGTGGCGTATGGCGGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGc                                                                     <  1:770386/238‑1 (MQ=255)
                                                                                                                                                                  gTTACC‑CCCAACGGGCGGGTGACCCACATGCCTAAGGCCCCGCTTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCAt                                                               <  1:732965/238‑1 (MQ=255)
                                                                                                                                                                   ttACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTg                                                             >  2:1014963/1‑239 (MQ=255)
                                                                                                                                                                       c‑accaGCGGGCGGGTGAACCAGATGCAGAAGTCACCGGTTTGCGTAGGGCGGTTGAAAAAGCAAAGGGGGGGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGt                                                         <  1:755154/239‑1 (MQ=255)
                                                                                                                                                                       c‑accaACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCCTCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCCGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACg                                                          >  2:501655/1‑238 (MQ=255)
                                                                                                                                                                             aCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGGAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCCAACCCTGTAGCCATCAAGGTCGGACGCCACCGCGCTGTGACGCACCCATCG‑CCGCTGGCGTAGCGCG‑CGGGGGTGCCCCGATCGAAGATCCCAACCCGCAGGGCGCTGGGCGGGGCCTTTTCCCTCTGCACAAGGCTGGCATTTACggtacc                                                     >  1:528621/1‑233 (MQ=255)
                                                                                                                                                                             aCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGc                                                     >  2:422892/1‑238 (MQ=255)
                                                                                                                                                                              cGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCg                                                                                 <  2:170967/210‑1 (MQ=255)
                                                                                                                                                                              cGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                   <  2:318621/239‑1 (MQ=255)
                                                                                                                                                                              cGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                   <  2:57986/239‑1 (MQ=255)
                                                                                                                                                                               gTGCGGGTGACCCACATGCCGAAGTACACGGGTTGCGTATGGCGGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                                   <  1:434510/238‑1 (MQ=255)
                                                                                                                                                                               gTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTTTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCAc                                                  >  2:581860/1‑239 (MQ=255)
                                                                                                                                                                                 gCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                 <  2:94618/238‑1 (MQ=255)
                                                                                                                                                                                 gCGGGTAACCCACGCGGCGAAGTACACGGGTTGCTTATGCCGGATGAAAAGCACAAGGGTGGGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACg                                                 <  1:1004939/238‑1 (MQ=255)
                                                                                                                                                                                  cGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGGCGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCCGGTCGCTGGGCGGGGGCCTTACCGTCTGCAACAGGCTGGCGTTGACGTCCGCCAccg                                                >  1:1033621/1‑236 (MQ=255)
                                                                                                                                                                                   ggggGAACCACATTGCGGAGCACAGGTTTTGCGGATGGCGGGTAAAAACGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                               <  1:312700/238‑1 (MQ=255)
                                                                                                                                                                                   gggTGAACCACATGCGGAAGTACCCGCGTTGCGTATGGCGGGTAAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCg                                                                                             <  2:78153/192‑1 (MQ=255)
                                                                                                                                                                                   gggTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                               <  2:804964/238‑1 (MQ=255)
                                                                                                                                                                                     gTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCCAAGGGGCGGCCGCCCATGTCACACGCGAAACCTGTAGGCAACATGGTCGGACGCCCCCCCACTGTGCGCCACCCGTCG‑CCCCGGGCGCGACGCG‑CGGGGGTGCCTCGTTGCAGCATCCCAACCCGCCGGACGCTCGGCGTGGACTCTAGCCACTGCGACAAGGAGGCGTTGACCGCAGCCAcccccgg                                            >  1:659660/1‑233 (MQ=255)
                                                                                                                                                                                      tGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtg                                            <  2:484125/238‑1 (MQ=255)
                                                                                                                                                                                        aaCCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAGCCAAAAGTGCGACCCCCTCTGTCACACCCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCCGCTGGCGTAGCGCG‑CGTGGGTGCCTCGATGCAAGATCCTAACCCGCCGGGCGCTGGGCGGGGGCCTTACCGTCTGCAACAg                                                                       >  1:170967/1‑210 (MQ=255)
                                                                                                                                                                                          ccACATGCCGAAGTCCCCTCGTGCCTTAGTGCGGCTAAAAAACCCAAAGGTCCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCtgatgat                                       <  1:404799/239‑1 (MQ=255)
                                                                                                                                                                                               tGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAAGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTg                                  >  2:1197381/1‑239 (MQ=255)
                                                                                                                                                                                                     agtccccgggtggCGTAGGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCg                            <  1:863849/227‑1 (MQ=255)
                                                                                                                                                                                                        cacggctgtGCGTATGGCGTGTGAAAAAGACAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                          <  1:258045/230‑1 (MQ=255)
                                                                                                                                                                                                        acaggtggtggggaggggggggaaaaaaGCCAAGGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                          <  1:206054/215‑1 (MQ=255)
                                                                                                                                                                                                         cacGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCa                        >  2:1152960/1‑239 (MQ=255)
                                                                                                                                                                                                           cgcgTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAAt                      <  2:1198881/239‑1 (MQ=255)
                                                                                                                                                                                                           cgcgTGGGGTATGGCGGGGAAAAAGGCCAAGGGGGCAACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAAt                      <  1:59114/239‑1 (MQ=255)
                                                                                                                                                                                                              gTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGGACGCCACCGTGCTGTGACGCACTCATCG‑CCGCCGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGGGGGGACTTTACCGTCTGCAACAGGGTGGCATTGACGTCAGCCACGGGCTGGTGGTGAGGGAAGCCGAGCAAATGa                   >  1:334893/1‑239 (MQ=255)
                                                                                                                                                                                                                 gCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATa                >  2:68633/1‑239 (MQ=255)
                                                                                                                                                                                                                      tGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGCTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCCACAGGCTGGCATTGACGTCAGACACGGCCTGGTGATGAGTGAAGCCGAGCAATTGAATAAAg             >  2:535493/1‑237 (MQ=255)
                                                                                                                                                                                                                            gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTtctc     >  2:817967/1‑239 (MQ=255)
                                                                                                                                                                                                                            gTGAAAAACACAAAGGTGCGACCGCCTATGTCACACTCGGACCCTGTAGCCATCATGGCCGGACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGGTGCCTCGATGCAGGCTCCCAACCCCCAGGTCGCTGGGGGGGGACTTTACCGTCTGCAACAAGCCTGCATTGACGTCGGCCACAGCCTGATGATGGGTGAAGCCGCGCAATTTAAAAAAAGCTTtct      >  1:645126/1‑238 (MQ=255)
                                                                                                                                                                                                                               aaaaaGCCGAAGGTCCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAg  <  1:1041943/239‑1 (MQ=255)
                                                                                                                                                                                                                               aaaaaGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAg  <  2:126538/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   
AAGAATTTGGCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACC‑ACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCG‑CCGCTGGCGTAGCGCG‑CGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGGCTTTCTCAAG  >  NC_000913/433414‑433874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A