Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,363	(G)_6→7	coding (256/729 nt)	gadW ←	transcriptional activator of gadA and gadBC, repressor of gadX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,357	1	.	G	97.1%	361.6 / 1.7	103	coding (262/729 nt)	gadW	transcriptional activator of gadA and gadBC, repressor of gadX
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (53/47); total (55/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 117 total aligned reads displayed.

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664219‑3664489

tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                        <  2:1363649/139‑1 (MQ=255)

tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                        <  2:646363/139‑1 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                                   >  1:1540547/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                                   >  2:634045/1‑139 (MQ=255)

     ttGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑ggggg                                                                                                                                  <  1:634045/139‑1 (MQ=255)

            gTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACt                                                                                                                           >  2:970411/1‑139 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGt                                                                                                                        >  1:1514855/1‑139 (MQ=255)

                    aTCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGttaatt                                                                                                                   <  2:1540547/139‑1 (MQ=255)

                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAAc                                                                                                                >  1:1035512/1‑139 (MQ=255)

                         ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACaa                                                                                                              <  2:942856/139‑1 (MQ=255)

                         ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCAGTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACaa                                                                                                              <  1:1479489/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCAATGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑ggggg                                                                                                                                  <  1:1240370/114‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:552111/1‑139 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  2:932166/139‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  2:215262/139‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:215262/1‑139 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:1759675/1‑139 (MQ=255)

                                     agaAAACATCGATAAACAGGAAAGGTAGAGCATATTTCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATAAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACggg                                                                                                         >  1:959760/1‑131 (MQ=255)

                                     agaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGt                                                                                                         <  2:959760/132‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:552111/139‑1 (MQ=255)

                                          aCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGGATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTa                                                                                             <  2:1463440/139‑1 (MQ=255)

                                              cGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAccc                                                                                         >  1:1024596/1‑139 (MQ=255)

                                                 tAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTaa                                                                                      >  1:1788353/1‑139 (MQ=255)

                                                 tAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTaa                                                                                      >  2:38481/1‑139 (MQ=255)

                                                  aaaCAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTaaa                                                                                     <  1:370406/139‑1 (MQ=255)

                                                     cAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTg                                                                                  <  1:377741/139‑1 (MQ=255)

                                                        gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g                                                                                                                                      <  2:1110827/84‑1 (MQ=255)

                                                        gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g                                                                                                                                      >  1:1110827/1‑84 (MQ=255)

                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          >  1:162707/1‑139 (MQ=255)

                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  2:655998/139‑1 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAAc                                                                                                            <  2:1566284/102‑1 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAAc                                                                                                            >  1:1566284/1‑102 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                       >  1:1447972/1‑139 (MQ=255)

                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggtga                                                                                                                             <  1:786337/83‑5 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACt                                                                                                                           >  2:560414/1‑80 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACt                                                                                                                           <  1:560414/80‑1 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGt                                                                               >  1:1783819/1‑124 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGt                                                                               <  2:1783819/124‑1 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          >  1:718589/1‑129 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  2:718589/129‑1 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  <  1:969264/137‑1 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  >  2:969264/1‑137 (MQ=255)

                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAatat                                                               >  1:1601310/1‑139 (MQ=255)

                                                                            cAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTg                                                           <  2:1678251/139‑1 (MQ=255)

                                                                             aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:576324/102‑1 (MQ=255)

                                                                             aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:576324/1‑102 (MQ=255)

                                                                             aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgt                                                          <  2:1623352/139‑1 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGc                                                                                                   >  1:1113918/1‑96 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑GGACTGGTTAATTAACAAACGGTCTTTGc                                                                                                   <  2:1113918/96‑1 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggtga                                                                                                                             >  2:504349/1‑66 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGATTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:496671/95‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGATTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:496671/1‑95 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACaa                                                                                                              >  1:744710/1‑80 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACaa                                                                                                              <  2:744710/80‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGt                                                                                                         <  1:970867/85‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGt                                                                                                         >  2:970867/1‑85 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTa                                                                                             >  1:482696/1‑97 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  2:211888/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  1:971639/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  2:1395550/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  2:1142179/1‑139 (MQ=255)

                                                                                       aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                <  1:1395550/139‑1 (MQ=255)

                                                                                       aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                <  2:23584/139‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGt                                                                                                         >  1:315949/1‑80 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGt                                                                                                         <  2:315949/80‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  >  2:1306876/1‑119 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  <  1:1306876/119‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 <  2:1322234/120‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 >  1:1322234/1‑120 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  2:1485968/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  2:1046933/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  1:640228/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCC‑AAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaaa                                             >  1:1373958/1‑139 (MQ=255)

                                                                                              cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         <  2:971639/139‑1 (MQ=255)

                                                                                                  gATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGc                                     <  2:1035512/139‑1 (MQ=255)

                                                                                                  gATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGGCTGACTGGTTAATTAAAAAACGGTCTTTGCTGTGTAACCCGAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGc                                     >  2:1143839/1‑139 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGTCTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTGTGTAAGTAATGGCAAACTGTCAGCt                                    >  1:825316/1‑139 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                    <  1:1046933/139‑1 (MQ=255)

                                                                                                      cATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCAt                                 <  1:1754556/139‑1 (MQ=255)

                                                                                                            aGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGtt                                                                                                                       >  1:784833/1‑47 (MQ=39)

                                                                                                            aGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGtt                                                                                                                       <  2:784833/47‑1 (MQ=39)

                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATc                          >  2:598698/1‑139 (MQ=255)

                                                                                                                aTCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                   <  1:926605/94‑1 (MQ=255)

                                                                                                                aTCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                   >  2:926605/1‑94 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:970838/66‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  1:844567/1‑66 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:970838/1‑66 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:844567/66‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCt                                                                                        >  1:1526721/1‑73 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCt                                                                                        <  2:1526721/73‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         <  1:969118/120‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      <  1:991527/139‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      >  1:730656/1‑139 (MQ=255)

                                                                                                                       aTCGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTcgc                >  1:351278/1‑139 (MQ=255)

                                                                                                                         cGCCGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGt              <  2:162707/139‑1 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAccc                                                                                          >  2:1002274/1‑61 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAcc                                                                                          <  1:1002274/61‑1 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  2:940491/77‑1 (MQ=255)

                                                                                                                           ccGTCACCAGCGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          >  1:940491/1‑77 (MQ=255)

                                                                                                                                     cGGCATGGGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACa  <  1:598698/139‑1 (MQ=255)

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGG‑TGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664219‑3664489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A