Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,363	(G)_6→7	coding (256/729 nt)	gadW ←	transcriptional activator of gadA and gadBC, repressor of gadX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,357	1	.	G	99.0%	381.8 / ‑3.6	104	coding (262/729 nt)	gadW	transcriptional activator of gadA and gadBC, repressor of gadX
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (53/50); total (53/51)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 118 total aligned reads displayed.

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑G‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664219‑3664489

tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                        <  1:710312/139‑1 (MQ=255)

tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                        <  1:338195/139‑1 (MQ=255)

   tATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑gg                                                                                                                                   <  1:37199/139‑1 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggg                                                                                                                                  >  2:309223/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggg                                                                                                                                  >  2:859439/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggg                                                                                                                                  >  2:364448/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggg                                                                                                                                  >  2:534620/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggg                                                                                                                                  >  1:97089/1‑139 (MQ=255)

    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATACTACTCAGTATCGGCGAATTTACCCCGGATTCATTGAAGCTATCAAAAATGGCCGTCACCAGCGGCAT‑g‑ggg                                                                                                                                  >  2:616386/1‑139 (MQ=255)

       gAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑gggggg                                                                                                                               >  1:1042305/1‑138 (MQ=255)

         aaaGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggggggtg                                                                                                                             <  1:223899/139‑4 (MQ=255)

           aGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCTGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggggggtgac                                                                                                                           <  1:769751/139‑6 (MQ=255)

           aGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑g‑ggggggtgac                                                                                                                           >  1:866259/1‑134 (MQ=255)

            gTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACt                                                                                                                           <  1:153709/139‑1 (MQ=255)

            gTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACt                                                                                                                           >  1:669312/1‑139 (MQ=255)

            gTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACt                                                                                                                           <  2:589783/139‑1 (MQ=255)

             tAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTg                                                                                                                          >  2:504631/1‑139 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        >  2:399883/1‑139 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        <  2:519602/139‑1 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        >  2:76732/1‑139 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        >  1:505181/1‑139 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        >  2:517787/1‑139 (MQ=255)

               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGAATCATTCAGGCTATCAACAATCGCCGTCACCAGCGGCAT‑GAGGGGGTGACTgtt                                                                                                                        <  2:566352/139‑3 (MQ=255)

                      cAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGttaattaa                                                                                                                 >  2:606244/1‑139 (MQ=255)

                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAAc                                                                                                                >  1:1029987/1‑139 (MQ=255)

                         ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACaa                                                                                                              <  1:76732/139‑1 (MQ=255)

                           ctttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAAAAAAc                                                                                                            >  2:585734/1‑139 (MQ=255)

                           ctttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCACAGTATCGGTGAATTTACCCCGGATTCGTTGAAGCTATCAAAAATCGCCGTCACCAGCGTCATGG‑GGGGGTGACTGGTTAATTAACAAAc                                                                                                            >  2:755483/1‑139 (MQ=255)

                            tttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACg                                                                                                           >  2:785468/1‑139 (MQ=255)

                             ttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTCACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACgg                                                                                                          <  1:118660/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         >  2:400782/1‑139 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  2:97089/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  2:962137/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  2:669312/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:502071/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  2:893018/139‑1 (MQ=255)

                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:20700/139‑1 (MQ=255)

                                 tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCtt                                                                                                      >  1:243674/1‑139 (MQ=255)

                                 tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCtt                                                                                                      >  1:472011/1‑139 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:141688/1‑139 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:28264/1‑139 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:806397/139‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:824560/139‑1 (MQ=255)

                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:102120/1‑139 (MQ=255)

                                       aaaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgt                                                                                                >  1:913056/1‑139 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:347899/139‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:141688/139‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:1042305/139‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:859439/139‑1 (MQ=255)

                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:364448/139‑1 (MQ=255)

                                               gATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCt                                                                                        >  2:83347/1‑139 (MQ=255)

                                                       ggAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCg                                                                                <  1:166805/139‑1 (MQ=255)

                                                       ggAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCg                                                                                <  2:493392/139‑1 (MQ=255)

                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  1:145756/139‑1 (MQ=255)

                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                          <  1:785468/139‑1 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                       >  2:913978/1‑139 (MQ=255)

                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                       >  1:640779/1‑139 (MQ=255)

                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:883586/113‑1 (MQ=255)

                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:883586/1‑113 (MQ=255)

                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCACCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                                     <  2:619151/139‑1 (MQ=255)

                                                                      atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 >  1:927885/1‑139 (MQ=255)

                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                >  1:1008947/1‑139 (MQ=255)

                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGt                                                                            >  2:834963/1‑126 (MQ=255)

                                                                          cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        >  1:869094/1‑80 (MQ=255)

                                                                          cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGt                                                                                                                        <  2:869094/80‑1 (MQ=255)

                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaa                                                        >  1:626194/1‑139 (MQ=255)

                                                                                   ggTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:687074/1‑91 (MQ=255)

                                                                                   ggTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:687074/91‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGa                                                                      <  1:31296/120‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGa                                                                      >  2:31296/1‑120 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  <  1:641451/124‑1 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  >  2:641451/1‑124 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  1:991689/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  2:545718/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  1:1044097/1‑139 (MQ=255)

                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                   >  1:556974/1‑139 (MQ=255)

                                                                                       aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagta                                                     <  2:193556/134‑1 (MQ=255)

                                                                                         tttACCCCGGTTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         >  2:749469/1‑80 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgt                                                          <  2:514647/127‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  1:1045573/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  2:834862/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  1:1019064/1‑139 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              <  2:243674/139‑1 (MQ=255)

                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGT‑AGTAATGGGaaa                                             >  2:699868/1‑139 (MQ=255)

                                                                                             ccccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTg                                          <  2:246672/139‑1 (MQ=255)

                                                                                              cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         >  2:354480/1‑139 (MQ=255)

                                                                                              cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         <  1:616386/139‑1 (MQ=255)

                                                                                              cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         >  1:68081/1‑139 (MQ=255)

                                                                                              cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         <  2:68081/139‑1 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                    <  2:28264/139‑1 (MQ=255)

                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                    <  1:400782/139‑1 (MQ=255)

                                                                                                       aTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         >  2:771713/1‑66 (MQ=255)

                                                                                                       aTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:771713/66‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGtt                                                                                                                       >  1:494822/1‑42 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      <  1:693934/139‑1 (MQ=255)

                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      >  1:18736/1‑139 (MQ=255)

                                                                                                                    aaaaTCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgt                   <  2:505181/139‑1 (MQ=255)

                                                                                                                      aaTCGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGtcgtcg                 >  1:421403/1‑139 (MQ=255)

                                                                                                                         cGCCGTCACCAGCGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGt              <  1:606244/139‑1 (MQ=255)

                                                                                                                                     cGGCATGG‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACa  <  1:504631/139‑1 (MQ=255)

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑G‑GGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664219‑3664489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A