breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,781,1520A→G13.7% 25.1 ‑41.5 ‑0.0183intergenic (+135/‑63)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
*NC_0009133,875,5210T→C17.0% 20.9 ‑9.1 0.0107A28A (GCT→GCCyidXputative lipoprotein
*NC_0009133,781,1400A→G12.2% 16.6 ‑37.9 0.0183intergenic (+123/‑75)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
*NC_0009133,875,5290C→G11.7% 14.5 ‑5.6 0.0112S31C (TCC→TGC) yidXputative lipoprotein
*NC_0009133,608,6820T→C11.1% 10.1 ‑8.8 0.083intergenic (+33/+69)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
*NC_0009133,426,9830G→T100.0% 2.0 1noncoding (51/76 nt)alaUtRNA‑Ala
*NC_0009133,619,4670C→G100.0% 1.3 1L92L (CTC→CTGrhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009132,997,5250A→G100.0% 0.8 1F26L (TTT→CTT) insC1IS2 repressor TnpA
*NC_0009132,997,5310G→T100.0% 0.8 1Q24K (CAG→AAG) insC1IS2 repressor TnpA
*NC_0009133,804,9160A→C100.0% 0.6 1D67E (GAT→GAGwaaSlipopolysaccharide rhamnose:KdoIII transferase; lipopolysaccharide core biosynthesis protein
*NC_0009131,634,4330T→A100.0% ‑0.5 1A151A (GCA→GCTtfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4340G→A100.0% ‑0.5 1A151V (GCA→GTA) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4350C→A100.0% ‑0.5 1A151S (GCA→TCA) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4370G→A100.0% ‑0.5 1A150V (GCT→GTT) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4380C→A100.0% ‑0.5 1A150S (GCT→TCT) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4400T→A100.0% ‑0.5 1D149V (GAT→GTT) tfaQQin prophage; putative tail fibre assembly protein

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009134,296,0600C→T18.1% 73.0 ‑0.4 ‑0.2155intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)24 (0.210) 20/292 5.6 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)18 (0.160) 13/284 7.6 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 3867985 =232 (1.970)7 (0.060) 6/284 10.9 3.1% coding (258/333 nt) yidQ DUF1375 family outer membrane protein
?NC_000913 3868014 = 216 (1.930)coding (287/333 nt) yidQ DUF1375 family outer membrane protein
* ? NC_000913 = 3775724287 (2.430)6 (0.050) 5/280 11.4 2.3% coding (153/588 nt) mtlR mannitol operon repressor
?NC_000913 = 3775808 238 (2.160)coding (237/588 nt) mtlR mannitol operon repressor
* ? NC_000913 307356 =96 (0.810)5 (0.040) 5/284 11.5 5.4% coding (1977/2526 nt) ecpC ECP production outer membrane protein
?NC_000913 307385 = 85 (0.760)coding (1948/2526 nt) ecpC ECP production outer membrane protein
* ? NC_000913 1045201 =108 (0.920)5 (0.050) 5/282 11.5 4.5% coding (603/1140 nt) gfcE putative O‑antigen capsule outer membrane auxillary protein export channel
?NC_000913 1045310 = 109 (0.980)coding (494/1140 nt) gfcE putative O‑antigen capsule outer membrane auxillary protein export channel
* ? NC_000913 3007598 =93 (0.790)5 (0.050) 5/268 11.1 5.6% coding (89/1197 nt) ygeX 2,3‑diaminopropionate ammonia lyase, PLP‑dependent
?NC_000913 3007614 = 87 (0.830)coding (105/1197 nt) ygeX 2,3‑diaminopropionate ammonia lyase, PLP‑dependent
* ? NC_000913 = 404121592 (0.780)5 (0.050) 5/280 11.4 5.4% coding (219/528 nt) mobB molybdopterin‑guanine dinucleotide biosynthesis protein B
?NC_000913 = 4041221 90 (0.820)coding (213/528 nt) mobB molybdopterin‑guanine dinucleotide biosynthesis protein B
* ? NC_000913 3927418 =96 (0.810)5 (0.050) 4/276 12.0 5.1% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 96 (0.880)coding (303/993 nt) asnA asparagine synthetase A
* ? NC_000913 = 4006603120 (1.020)5 (0.040) 4/286 12.3 4.0% coding (740/1830 nt) recQ ATP‑dependent DNA helicase
?NC_000913 = 4006647 125 (1.110)coding (784/1830 nt) recQ ATP‑dependent DNA helicase
* ? NC_000913 = 49333379 (0.670)4 (0.040) 4/284 12.2 4.9% coding (1242/1932 nt) dnaX DNA polymerase III/DNA elongation factor III, tau and gamma subunits
?NC_000913 = 493336 79 (0.710)coding (1245/1932 nt) dnaX DNA polymerase III/DNA elongation factor III, tau and gamma subunits
* ? NC_000913 = 3693567277 (2.350)4 (0.040) 4/284 12.2 1.5% coding (1670/2619 nt) bcsA cellulose synthase, catalytic subunit
?NC_000913 = 3693598 247 (2.210)coding (1639/2619 nt) bcsA cellulose synthase, catalytic subunit
* ? NC_000913 = 3739543221 (1.870)4 (0.040) 4/280 12.1 1.9% intergenic (+16/‑162) malS/avtA alpha‑amylase/valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
?NC_000913 = 3739556 218 (1.980)intergenic (+29/‑149) malS/avtA alpha‑amylase/valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
* ? NC_000913 = 3876768115 (0.970)4 (0.040) 4/284 12.2 3.5% coding (185/813 nt) yidA sugar phosphate phosphatase
?NC_000913 = 3876814 110 (0.990)coding (139/813 nt) yidA sugar phosphate phosphatase
* ? NC_000913 4542682 =5 (0.040)4 (0.040) 4/282 12.2 13.4% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 47 (0.420)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 3998038 =103 (0.870)5 (0.040) 3/286 13.1 5.0% coding (56/2163 nt) uvrD DNA‑dependent ATPase I and helicase II
?NC_000913 3998077 = 91 (0.810)coding (95/2163 nt) uvrD DNA‑dependent ATPase I and helicase II
* ? NC_000913 4516891 =104 (0.880)5 (0.050) 3/282 12.9 4.9% coding (827/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
?NC_000913 4516925 = 98 (0.880)coding (793/954 nt) fecR anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
* ? NC_000913 1143957 =135 (1.140)4 (0.040) 3/272 12.7 3.1% coding (411/3186 nt) rne endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
?NC_000913 1143971 = 129 (1.210)coding (397/3186 nt) rne endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
* ? NC_000913 3858193 =232 (1.970)4 (0.040) 3/274 12.7 1.9% coding (212/1494 nt) yidJ sulfatase/phosphatase superfamily protein
?NC_000913 3858242 = 208 (1.930)coding (163/1494 nt) yidJ sulfatase/phosphatase superfamily protein
* ? NC_000913 4255890 =138 (1.170)4 (0.040) 3/284 13.0 2.9% coding (577/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
?NC_000913 4256077 = 139 (1.240)coding (390/2424 nt) plsB glycerol‑3‑phosphate O‑acyltransferase
* ? NC_000913 = 525155106 (0.900)3 (0.030) 3/286 13.1 2.9% coding (1895/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3621044 NA (NA)coding (1853/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 732856 =113 (0.960)3 (0.030) 3/286 13.1 2.7% coding (3274/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3622440 = NA (NA)coding (3249/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 1285349110 (0.930)3 (0.030) 3/284 13.0 2.7% coding (211/711 nt) narJ molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
?NC_000913 = 1285396 111 (0.990)coding (258/711 nt) narJ molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
* ? NC_000913 = 1428843NA (NA)3 (0.030) 3/284 13.0 2.7% pseudogene (48/189 nt) lomR pseudogene, Rac prophage lom homolog;Phage or Prophage Related; interrupted by IS5 and N‑ter deletion
?NC_000913 = 1428931 108 (0.970)pseudogene (136/189 nt) lomR pseudogene, Rac prophage lom homolog;Phage or Prophage Related; interrupted by IS5 and N‑ter deletion
* ? NC_000913 1739638 =90 (0.760)3 (0.030) 3/284 13.0 3.4% coding (161/933 nt) ydhB LysR family putative transcriptional regulator
?NC_000913 1739666 = 84 (0.750)coding (133/933 nt) ydhB LysR family putative transcriptional regulator
* ? NC_000913 1997022 =73 (0.620)3 (0.030) 3/280 12.9 4.0% intergenic (‑190/+40) sdiA/yecC quorum‑sensing transcriptional activator/putative ABC transporter ATPase
?NC_000913 1997051 = 77 (0.700)intergenic (‑219/+11) sdiA/yecC quorum‑sensing transcriptional activator/putative ABC transporter ATPase
* ? NC_000913 = 231872297 (0.820)3 (0.030) 3/284 13.0 2.8% coding (1155/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
?NC_000913 = 2318773 116 (1.040)coding (1104/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
* ? NC_000913 = 2319247130 (1.100)3 (0.030) 3/284 13.0 2.4% coding (630/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
?NC_000913 = 2319264 125 (1.120)coding (613/2850 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
* ? NC_000913 = 2331858121 (1.030)3 (0.030) 3/284 13.0 2.5% pseudogene (2545/4605 nt) yfaS pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
?NC_000913 = 2331859 116 (1.040)pseudogene (2544/4605 nt) yfaS pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
* ? NC_000913 = 2593882134 (1.140)3 (0.030) 3/286 13.1 2.3% coding (1978/2016 nt) tmcA elongator methionine tRNA (ac4C34) acetyltransferase
?NC_000913 = 2593918 126 (1.120)coding (1942/2016 nt) tmcA elongator methionine tRNA (ac4C34) acetyltransferase
* ? NC_000913 = 3381847114 (0.970)3 (0.030) 3/282 12.9 2.7% coding (1105/1368 nt) degQ serine endoprotease, periplasmic
?NC_000913 = 3381859 106 (0.960)coding (1117/1368 nt) degQ serine endoprotease, periplasmic
* ? NC_000913 = 3621044NA (NA)4 (0.040) 3/286 13.1 4.6% coding (1853/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3621074 87 (0.740)coding (1883/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 3781125184 (1.560)3 (0.030) 3/290 13.2 1.7% intergenic (+108/‑90) lldD/trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
?NC_000913 = 3781138 179 (1.570)intergenic (+121/‑77) lldD/trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
* ? NC_000913 = 3858205230 (1.950)3 (0.030) 3/274 12.7 1.4% coding (200/1494 nt) yidJ sulfatase/phosphatase superfamily protein
?NC_000913 = 3858228 208 (1.930)coding (177/1494 nt) yidJ sulfatase/phosphatase superfamily protein
* ? NC_000913 = 3894310133 (1.130)3 (0.030) 3/284 13.0 2.3% coding (430/750 nt) yieE phosphopantetheinyl transferase superfamily protein
?NC_000913 = 3894317 129 (1.160)coding (437/750 nt) yieE phosphopantetheinyl transferase superfamily protein
* ? NC_000913 = 3895233122 (1.030)3 (0.030) 3/276 12.8 2.6% intergenic (+15/+39) chrR/adeP chromate reductase, Class I, flavoprotein/adenine permease, high affinity; adenine:H+ symporter
?NC_000913 = 3895244 116 (1.070)intergenic (+26/+28) chrR/adeP chromate reductase, Class I, flavoprotein/adenine permease, high affinity; adenine:H+ symporter
* ? NC_000913 4289016 =142 (1.200)3 (0.030) 3/282 12.9 2.2% coding (1253/1437 nt) nrfA nitrite reductase, formate‑dependent, cytochrome
?NC_000913 4289092 = 138 (1.240)coding (1329/1437 nt) nrfA nitrite reductase, formate‑dependent, cytochrome