Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F111 I2 R1	11	49.6	1720206	93.9%	1615273	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	90.6 / NA	34	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/18); total (16/18)

TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132652‑2132925

tggtggAGGGTGAACAGCTGATATTTTGTGCTGGCGCGGTCGAACATACAGCTCAATTTTCTGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                     <  2:512362/143‑1 (MQ=255)

gggTAGGGGGGAAAGAGCTGCTATTTTTTGCTGGCGCGGTCGAAGTTGTCGTGGTGCGTGCGGTAAAAGTGGACGTGCACGGAGAATTTTTTCGGTG‑ACGTGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                     <  2:529049/142‑1 (MQ=255)

 ggTAGAGGGTAAACAGCTAATATATTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTG‑ACGAGGTGATCTGCATTTAACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                    >  2:522581/1‑143 (MQ=255)

   tAGAGGGTAAGCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  1:712715/143‑1 (MQ=255)

   tAGAGGGTAAACAGTGGAGATTTATTGCTAGCGCGGTCTAATTTGTCTTTGCGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  2:559165/143‑1 (MQ=255)

           aagcAGCTGATATTGTTGGCTGGCGCGGTCGGAGTTGGCTTTCTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                          <  2:124973/140‑1 (MQ=255)

           aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTCTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                          <  1:278674/143‑1 (MQ=255)

                  tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   >  2:584176/1‑143 (MQ=255)

                  ggATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:167926/142‑1 (MQ=255)

                           cggCTGGCGCGGGCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTCACCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                          <  1:769450/141‑1 (MQ=255)

                                         gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacca                                                                                            >  2:715074/1‑143 (MQ=255)

                                         gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATATCACCGCGAATGATATGCAGGCTCCGCCTCGCCCCTTCTACTTTCCACGCTTAGACGGACTccaccccca                                                                                            >  2:666773/1‑143 (MQ=255)

                                                      tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGCAGCAATCGTTGCAAAG‑TGATGGCCGTGACTACGCCGCTGGCTCTGCGTCGCCCCTTCTGCTGTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAAggt                                                                               >  1:396938/1‑141 (MQ=255)

                                                         ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                            <  1:722305/143‑1 (MQ=255)

                                                             ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGCCGTACTCCACCACCACACGCAGGAAGTAGTCGTaa                                                                        >  2:780962/1‑143 (MQ=255)

                                                             ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                        <  1:155519/143‑1 (MQ=255)

                                                                   aGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                  >  1:179241/1‑143 (MQ=255)

                                                                    gTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATATGCATTTCACAGGGATTGATATGCAAGCTCTGCGTCGCCTCTTCTACTTTCCACAGTTCTCCGTACTCCACCACCATCCGCAGCAAGATGTCGTAAACCggtg                                                                 >  2:237054/1‑143 (MQ=255)

                                                                       gAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCg                                                              <  1:416532/143‑1 (MQ=255)

                                                                       gAAGTACCCGGAGAATTTTTTCGGTG‑ACAAGATGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGACATACTCCACCACCATCAGCAGGAAGATGTCGTAATccactccag                                                              >  2:509784/1‑136 (MQ=255)

                                                                            aCCCGGAGAATTTTTTCTGTG‑TCTAGTTGTTCTGCATTTCACCGTGATTGTTATTCAGGCTCTGCGTCGGCGCATCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTc                                                         <  1:715074/143‑1 (MQ=255)

                                                                               cGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAGGATGTCGTAAGTCTGTGCCGGTTTCGGt                                                      >  1:634849/1‑143 (MQ=255)

                                                                                       tttttCGGTG‑ACGAGGTGCTCTCCATCTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                              <  1:154717/143‑1 (MQ=255)

                                                                                          ttCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                           <  1:340683/143‑1 (MQ=255)

                                                                                           tCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGaac                                          >  1:89259/1‑143 (MQ=255)

                                                                                            tcggccacgccgTGATCTGCACTTCCCCGTGATTCACATGCAGCCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                          <  1:94928/132‑1 (MQ=255)

                                                                                                      ggTGATCGCCATTCCGCCGTGGTTGAGATGCAGGCTCTGCGTCGCTCGGTCGACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTGCGGTATCGAACAAACACGCtt                                <  2:546058/143‑1 (MQ=255)

                                                                                                             tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCCTTCAGTCCGGTACCGGACAAACAGTCTCTGAAACg                         >  2:775791/1‑143 (MQ=255)

                                                                                                               cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCCCTTGCAGCTCAGCATAGAACAAACACTCTCTGAACCGcc                       >  2:785450/1‑143 (MQ=255)

                                                                                                                aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                      >  1:774876/1‑143 (MQ=255)

                                                                                                                 tttCACGGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAGTTTCGACGGTTCGCCGTACTCCACCAGCATCCGCAGGAAGATGTGGTAATCCTGTGCGGCGTTCAGTTAGGTAGCGAACTAACCATCTTTGAACCGCCag                     >  2:620807/1‑142 (MQ=255)

                                                                                                                     aCCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                 <  2:231064/143‑1 (MQ=255)

                                                                                                                         tcattgatATGCAGGCCCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCGTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt             <  2:467361/141‑1 (MQ=255)

                                                                                                                                    cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGCCCTGGt  >  2:190497/1‑143 (MQ=255)

TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTG‑ACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132652‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A