Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I1 R1	11	69.2	2403364	94.6%	2273582	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	129.2 / NA	47	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (22/25); total (22/25)

GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132654‑2132922

gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                >  1:1096736/1‑143 (MQ=255)

           aCAGCTGAGATTTTTTGCCGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                     <  1:335658/143‑1 (MQ=255)

                tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                >  1:247903/1‑143 (MQ=255)

                 gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                               >  1:541587/1‑143 (MQ=255)

                  atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                              <  1:766736/143‑1 (MQ=255)

                  atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                              >  2:454947/1‑143 (MQ=255)

                       ttttGCTCGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTCTTCGGTGTCTATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg                                                                                                         <  1:447953/143‑1 (MQ=255)

                          tGCTGGCGCGGTCTCATTTGTCTTTGTCCTTGCGGTACACGTCCAAGTACACGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                      <  1:679462/143‑1 (MQ=255)

                             tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                   >  1:80087/1‑143 (MQ=255)

                                          tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCCTACTCCACCCCCAGcc                                                                                      >  2:171744/1‑143 (MQ=255)

                                              tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                  >  1:854798/1‑143 (MQ=255)

                                              tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                  <  1:570958/143‑1 (MQ=255)

                                                tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCACGGTTCGCCGTAATCCACCACCATCCGCCggc                                                                                >  2:870952/1‑142 (MQ=255)

                                                tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGa                                                                                >  1:1032957/1‑143 (MQ=255)

                                                 gtgtgCTGGCGGGAAACGGGGAAGTACCCGGAGAATTTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                               <  2:936109/142‑1 (MQ=255)

                                                       ttacGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                         <  1:751864/140‑1 (MQ=255)

                                                           ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTaa                                                                     >  1:776303/1‑143 (MQ=255)

                                                           ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACAACAGCAAGAAAATGTCATaa                                                                     >  2:960789/1‑143 (MQ=255)

                                                             tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTAATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCATCGACCACCATCCACAGGAAGACAGCGTAATc                                                                   >  2:1042043/1‑143 (MQ=255)

                                                              aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGACGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATcc                                                                  <  2:660145/143‑1 (MQ=255)

                                                               aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTTTTCCt                                                                 >  2:126621/1‑143 (MQ=255)

                                                                 aggggAAGTGCGCGGGGAAGTTTGTGGGTGACGGGGTGATCTGCAGTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                               <  2:698884/140‑1 (MQ=255)

                                                                 aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                               >  1:785903/1‑143 (MQ=255)

                                                                  gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                              >  1:917232/1‑143 (MQ=255)

                                                                             cGGCGAATTTTGTCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                   <  2:529138/143‑1 (MQ=255)

                                                                             cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGt                                                   >  1:572873/1‑143 (MQ=255)

                                                                                    ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGCCTCCGCGTCGCCTCTTCTACTTTCCACGGTTCGCCACACTACCCCACCATCCACAGGCAGATGTCGTAATCCTGTGCCGCCTCCAggccgctat                                            >  2:12083/1‑135 (MQ=255)

                                                                                         tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                       <  1:197966/143‑1 (MQ=255)

                                                                                           ggTGGCGCGGTGATCTGCATTGCACCGCGATTGAGGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                     <  2:755784/143‑1 (MQ=255)

                                                                                                 gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                               <  1:27944/143‑1 (MQ=255)

                                                                                                 gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCTTCAGTTCGGCACCGAACAAacaccc                               >  2:846886/1‑141 (MQ=255)

                                                                                                    gggATGTGCATGTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTATCGAACAAACACTCttt                            <  2:121827/141‑1 (MQ=255)

                                                                                                      gATCTGCGTTGCTCTGCGATTGATGTGCAGGCTGTGCGTGGCGCCTTCTACTTTCCAGGGTTCGCCGGCCTCCACCACCTGCCGCGGGACGATGTCGTAGTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                          <  2:527603/143‑1 (MQ=255)

                                                                                                      gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                          <  2:803793/143‑1 (MQ=255)

                                                                                                           gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAGAAACACTCTTTGAAACGc                     >  1:375316/1‑143 (MQ=255)

                                                                                                            cATTTCACCGTGATTGATCGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc                    <  2:630430/143‑1 (MQ=255)

                                                                                                             ttttCCCCGTGATTGATCGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCTCGGTTCGCCGTACTCCACCTCCATCCGCTGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                   <  2:50361/142‑1 (MQ=255)

                                                                                                             atgtctccGTGAGTGGTATGGAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                   <  2:633880/137‑1 (MQ=255)

                                                                                                             aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                   >  1:506810/1‑143 (MQ=255)

                                                                                                              ttgcACCGTGGTGGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCGTTCTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt                  <  2:452136/140‑1 (MQ=255)

                                                                                                                tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGAAACGCCATGc                >  1:154020/1‑143 (MQ=255)

                                                                                                                    cGTGATTGATATGCAGGCTGTGCGTCGCCGCTTCGACTTTCCAGGGTCCGCCGTGCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg            <  2:825136/143‑1 (MQ=255)

                                                                                                                    cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGCTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTACAGCTCGGTATCGAACAGACACGCTTTGAAACGGCATGccgcg            >  2:737415/1‑140 (MQ=255)

                                                                                                                        attgatCTGCAGGCTCTGCGTCGCCTCTCCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaa        <  1:285940/143‑1 (MQ=255)

                                                                                                                            atctGCAGGCTGTGCGCCCCCTCTTCCGCTTTCCACTGTTCGCCGTACTGCAACACCATCCGCAGGAAGATGTCGTATTTCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTTTTTGAAACGCTATGCCCAGGTAAAGAc    <  2:959251/140‑1 (MQ=255)

                                                                                                                             tgtGCAGGCTTTGCGTCGCCGCGTCTACTTTCCGCGGTTCGCCGTACGCCACCACCAGCCGCAGGAAGATGGCGTAATCCGGGGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAcc   <  2:539453/141‑1 (MQ=255)

                                                                                                                              atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt  <  1:241650/143‑1 (MQ=255)

GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132654‑2132922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A