Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F110 I1 R1
|
9 |
45.0 |
1568152 |
94.0% |
1474062 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,132,787 |
A→C |
I204S (ATC→AGC) |
wcaA ← |
putative glycosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0%
| 56.8
/ NA
| 21 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (11/10); total (11/10) |
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132648‑2132925
|
gaccTGGTCGAGGTAAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTGTGGGCTTGCGGTAAAAGTGGAAGTCCCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct < 1:274583/140‑1 (MQ=255)
tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCTGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc < 2:450346/143‑1 (MQ=255)
gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg > 1:616890/1‑143 (MQ=255)
tGATATGTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCa < 2:475308/143‑1 (MQ=255)
tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:79061/1‑143 (MQ=255)
tGGCGCGGTCGAATGTGTTTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc < 2:20440/143‑1 (MQ=255)
gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAGTc > 1:207150/1‑143 (MQ=255)
cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac < 2:170515/143‑1 (MQ=255)
gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCGGGAAGa > 2:456357/1‑143 (MQ=255)
gCTTGCGGTAACAGTGGTAGTACCCGGAGAATTTTTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg < 2:354833/143‑1 (MQ=255)
tggCGGTAGAGTTGGAAGTATCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:573993/141‑1 (MQ=255)
ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACACCCGCAGGCCGCCGTCGTaa > 2:516507/1‑143 (MQ=255)
agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:45230/143‑1 (MQ=255)
tccgTGAGGGGGTGGCCTGCCTGTCACCGCGGTTCATATGCACGCGCCGCTTCGTCTCGTATGCTTACAACCGTTCGCCGTACTCCACCACCACGCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac < 2:375191/140‑1 (MQ=255)
gggACGGGGTGATCTTCTGGTCACTGTGATTTATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCATCGGTTGTGCGTTGTGCATTATGATGCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTATCGaacaaa < 2:77837/141‑1 (MQ=255)
tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac > 1:643417/1‑143 (MQ=255)
aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAACCACTCTTTGaa > 1:117577/1‑143 (MQ=255)
tCTGCATTTCACCGTGATTAATAGGCAGGCTCTGCGCCGCCCCCTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa < 1:695183/143‑1 (MQ=255)
tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGAAACg > 2:622976/1‑143 (MQ=255)
tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTCCGGTACCGAAAACCAACTCTTTGAAACg > 2:84832/1‑143 (MQ=255)
gCATTTCACCGTGATTAATATGCAGGCTATGCGTCGCCTCTTCTACTTTACACGGTTAGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCATGTGCCTCTTTCACTTCGATATAAAACGAACACTATATGacaaaa > 2:402936/1‑138 (MQ=255)
cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTCAAACGCCATGCCCAGGTAAAGACCTGGt > 2:201112/1‑143 (MQ=255)
|
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT > NC_000913/2132648‑2132925
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
