Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F110 I2 R1
|
8 |
55.0 |
1914758 |
94.7% |
1813275 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,132,787 |
A→C |
I204S (ATC→AGC) |
wcaA ← |
putative glycosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.0%
| 84.9
/ NA
| 33 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (16/16); minor base G (0/1); total (16/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132648‑2132926
|
gattttgTAGAGGGTAAACAGCTGATATTTTTTGCTGTCGCGGTCGAATTTTTCTTTGTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct < 1:899656/137‑1 (MQ=255)
gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCg > 1:44364/1‑143 (MQ=255)
tAGAGGGTAAACAGCGGATGTTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 2:553254/143‑1 (MQ=255)
gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCAGGAGAATATTTTCGGTGACGAGGTGATCTGAATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCctct > 1:828742/1‑143 (MQ=255)
ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 1:611913/143‑1 (MQ=255)
ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCtctt > 1:778929/1‑143 (MQ=255)
atcTGATATGTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt < 2:779835/141‑1 (MQ=255)
tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGCTCTGCGTCGGCTCCTCGACtttttg > 2:210692/1‑140 (MQ=255)
tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTTTCCa > 1:675351/1‑143 (MQ=255)
atTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt < 1:212112/143‑1 (MQ=255)
ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 2:190584/1‑143 (MQ=255)
tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtac > 2:469400/1‑141 (MQ=255)
gcgcGGTCGAGTTTGGCTTTGTGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc < 1:869784/143‑1 (MQ=255)
tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac > 2:778656/1‑143 (MQ=255)
cGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:920026/143‑1 (MQ=255)
cGAATCTGTCTGTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc < 2:670624/143‑1 (MQ=255)
atttatgggtgtgCTTGCGGTAAAGCTCGAACTACCCGGAGAATTTTTTCCGTGACGAGGTTATCTGCATTTCACCGTGATTGATATTCAGGCTCTGCCTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc < 2:872779/134‑1 (MQ=255)
tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg < 2:264945/143‑1 (MQ=255)
tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt < 2:601722/143‑1 (MQ=255)
ttGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 2:404397/143‑1 (MQ=255)
cgTGGCAGCACCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGAGTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt < 1:210692/142‑1 (MQ=255)
tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt > 1:629310/1‑143 (MQ=255)
ccccGAGCATTTGTGCGGTGCCGAGGTGATTTGCATTGCAGCGTGATTGATATGCAGGGTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:195443/143‑1 (MQ=255)
ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTGCACCACCAACCACAGGATGATGTCCCAATCCTGTGCCGCTTTCAGTTCCGTAt > 1:877952/1‑143 (MQ=255)
tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCAGGCTCTGCTTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTACTCCACCCACCGCAGGAAGATGTGCTAATCCTCTCCCCCTTTCACttgtggttc > 1:368084/1‑136 (MQ=255)
tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGa > 1:153624/1‑143 (MQ=255)
ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa < 2:622650/143‑1 (MQ=255)
ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAATATCCACCCGTCGCCGTAGTCCACAACCATCCGCAGTAAGATGTCGTAATCCAGTGCCGCTGTCAGTTCGGTATGGaacag > 1:518688/1‑142 (MQ=255)
gggATCTGTCTTCCACCGGGCTTGATTTGCAGGCGCTGCGTCGCCTCTTCTACTTTCCCCGCTCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 1:326839/141‑1 (MQ=255)
tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCTGCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg < 1:104558/143‑1 (MQ=255)
gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCCGTTCGGGACCGAACAAACACTCTTTGGAACGCCACGCCCAgg > 1:669390/1‑143 (MQ=255)
attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACGATCCGCAGGAAGATGTCGTAATCCTGTGCGGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGGaa > 2:100826/1‑143 (MQ=255)
ggCAGGCTCGGCGTCGGCTCTGCTAGTTGCCGCGGTTCGCCGTCCGCCACCACCGTCCGCAGGAAGATGTCGGAATCCTGGGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg < 1:234980/142‑1 (MQ=255)
aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGTTTTCAGTTCGGGATCCAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGCtt > 2:851856/1‑143 (MQ=255)
|
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132648‑2132926
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
