Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F17 I0 R1 155 84.1 1797446 90.9% 1633878 230.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC98.9% 291.8 / ‑6.1 98I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (46/50);  minor base T (2/0);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGC  >  NC_000913/2132557‑2133021
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
tttGGCCCCCCCGAATACCATCACCCATACGTTTGCCGTTACGCACCGCCAGCAGCGTCAGGAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                                    <  1:451532/239‑1 (MQ=255)
tttAGTGCCCCCGGATACCTCGAGGCAAGCGATTGCCGTTACGCACCGGCAGCAGCGGCAGTGAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                                    <  2:836157/239‑1 (MQ=255)
                    tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCCCgg                                                                                                                                                                                                                >  2:786486/1‑239 (MQ=255)
                    tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGACCTGCATTTCACCGGGATTGAAATGCAGGCTCGGGGTCGCCCCTCTAACTTTCCACgg                                                                                                                                                                                                                >  1:420064/1‑239 (MQ=255)
                    tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGGGACGAGGTGATCTGCATTTCACCGTGATTGAAATCCAGGCTCTGCGTAGCCTCTTCTACTTTCCACgg                                                                                                                                                                                                                >  2:839001/1‑239 (MQ=255)
                      aGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGGGACGAGGTGATCTGCATTTAACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGt                                                                                                                                                                                                               >  2:572804/1‑238 (MQ=255)
                       gCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGAATTTCACCGTGATTGATATGACGGCCCTGCGTCGCCCCTTCACCTTTCCACgttac                                                                                                                                                                                                             >  1:831509/1‑235 (MQ=255)
                           gACGTTTGCCGTTACGCACCGGCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑TGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                         <  2:369683/239‑1 (MQ=255)
                            aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGGGATGGATATGCAGGCTCTGCGTCGCCACTTCTCCTTTCACCGGTTGCCCGt                                                                                                                                                                                                        >  1:859999/1‑239 (MQ=255)
                            aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACAGGACTTGGTATGCAGGCTCGGCGTTCCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                         >  1:311040/1‑238 (MQ=255)
                              gTTTGCCGTTACGCACCGACAGGAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                                                                                                                       <  2:657631/238‑1 (MQ=255)
                                tgtgcGTTACGGACGCACAGCAGGGTCAGCAAAGTGCGCGGGGTCTACCGTTTATGGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTGTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                                                                                                                    <  1:286515/235‑1 (MQ=255)
                                 tGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGTTCTGCGTGGCCTCTTCTCCTTTCCACGGTTCTCCgaattc                                                                                                                                                                                                    >  2:547574/1‑233 (MQ=255)
                                 tGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc                                                                                                                                                                                                   >  2:429392/1‑239 (MQ=255)
                                                  agcgggggcAGGAAAGGGCGCCAGGTTATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCCCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                                                                                                                  <  1:478572/231‑1 (MQ=255)
                                                    cggcgtccgtaaatggtgtcAGGTCATACGTTTTTTGCGGCTCTGGTAGAGGGTAAACAACTGA‑TATTTTTTGCTGGCGCGGTTGAATTTGTCTGTGTGCTTGCGTTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGa                                                                                                                                                                                <  1:114052/220‑1 (MQ=255)
                                                     cgggTCAGCAAAGGTCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                                                <  2:98670/235‑1 (MQ=255)
                                                     agggTCAGCAAAGTTGGCCAGGTAATGCGTTTATTGCGGATCTGGGTGAGGGTAAACAGCTGA‑TATTTGTTGCTGGCGGGGTCGAATTTGTTTTTGTACTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCGCCACCATCCGCAGGAAGa                                                                                                                                                                                <  1:154216/235‑1 (MQ=255)
                                                     agcGTCTGCAAAGTGCGCCAGGTCTTACTTTTATTGCGGATCTTGTAGGGGGTAAACAGCTGA‑TATTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                                                <  1:191030/238‑1 (MQ=255)
                                                     agcGTCAGGAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                                                                                                                                 <  2:225372/237‑1 (MQ=255)
                                                         tCAGCAAAGGGCGGCAGGTCATGCGTTTATTGCGGATCTGGTAGGGGGTAAACAGTTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                                                                                                                           <  1:641089/239‑1 (MQ=255)
                                                            gCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGGATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacctggttcttatacacatctccgac                                                                                                                                                                         >  2:747220/1‑215 (MQ=255)
                                                            gCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                                                                                                                        >  2:461225/1‑239 (MQ=255)
                                                                 gTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATTTCGTAATCCtt                                                                                                                                                                   >  2:788053/1‑238 (MQ=255)
                                                                    cgcCAGGTCATACGTTTATTGGGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                                                                                                                 <  2:484966/238‑1 (MQ=255)
                                                                    cgcCAGGGCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGGGACGCGGTGATCTGAATTTCACCGTGATTGTTTTGCAGGCTATGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGGCCTCCACCCCCGACCGCAGGCAACAGTCGTAATCTtgtg                                                                                                                                                                 >  1:154062/1‑238 (MQ=255)
                                                                           tcagtcGTTTATTGCGGATCTGGTAGAGTGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                                                                                                                          <  2:522365/233‑1 (MQ=255)
                                                                           gcgtgggTTTTTTGGGGTTCTGGTAGAGGGTAAGCGGGTGA‑TATTTTTTGCTGGCGCGGGTGGATTTGTCTTGGTGCTTGGGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                                                                                                                          <  1:649559/232‑1 (MQ=255)
                                                                            cATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCCCAGGAAGATTTCGTAATCCTTTCCCCCTTTCa                                                                                                                                                        >  1:484966/1‑239 (MQ=255)
                                                                              tACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                                                                                                                      >  1:259818/1‑239 (MQ=255)
                                                                               gttttttttggggattgggtgaggggTAAACGGGCGG‑TATTTTTTGCGGGCGCGGTCGAATTTGTTGTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                     <  1:47521/216‑1 (MQ=255)
                                                                               gcGTTTATTGCGGATCTGGTGGGGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                     <  2:684826/238‑1 (MQ=255)
                                                                               aCGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                     <  2:420621/239‑1 (MQ=255)
                                                                               aCGTTTATTGCGGATCGGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTTTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGACGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                     <  2:265197/239‑1 (MQ=255)
                                                                                   ttATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt                                                                                                                                                 >  2:117144/1‑239 (MQ=255)
                                                                                    tATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTa                                                                                                                                                >  2:605428/1‑239 (MQ=255)
                                                                                    tATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                                                                                                                      >  2:283598/1‑217 (MQ=255)
                                                                                     tttgtggattagtgtgagGTTAAACAGCTTA‑TATTTTTGGCGGGGGCTGTCGAATTTGCTGTTGTGCTTGCGGTAAAAGTTGAAGTACCCGGAGAATTGTTTC‑GGTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                                                                                                               <  1:306110/224‑1 (MQ=255)
                                                                                     tttGCGGTTCTTGTGGAGGGTAAACAGCTGA‑TTTTTTTGGCGGGCGTGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                                                                                                               <  1:739580/238‑1 (MQ=255)
                                                                                           gATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCCCTTTCACTTCGGAATCGaaca                                                                                                                                         >  1:564699/1‑239 (MQ=255)
                                                                                           gATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGGCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaaa                                                                                                                                         >  1:609086/1‑237 (MQ=255)
                                                                                             tttgttggagGGTAAGCAG‑TGATTATTTTTGGCGGGCGCGGGGGAATTTTTCTTTGGGCATGGGGTAAAAGTGGAAGTCCCCGGGAAATTTTTTC‑GGTGACGAGGGGATCTGCATTTCACCGGGTTTGATATGCAGGCTCTGCGTCGCCTTTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                                                                                                                       <  1:212957/232‑1 (MQ=255)
                                                                                              cTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                                                                                                                       <  2:649834/238‑1 (MQ=255)
                                                                                                      gggTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg                                                                                                                              >  2:669432/1‑239 (MQ=255)
                                                                                                      gggTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGGGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCTTCGCCCCTTCTCCTTTCCCCGGTTCGCCGTACAGCACCACAATCCGCCGGAAGAAGTCGTAATCCTGTGCCGCCTTCAGTTTCGAATCGAACAAACACCCTTTg                                                                                                                              >  1:238966/1‑239 (MQ=255)
                                                                                                       ggTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTCCATTTCGGAATCAACCAAACACTCTTTGa                                                                                                                             >  2:598583/1‑239 (MQ=255)
                                                                                                         tAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCAGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCACTTCGGCTTCGAACAAACCCCCTTTGaa                                                                                                                            >  1:340110/1‑238 (MQ=255)
                                                                                                          gtagctgttgttatTTTTTGCGGCGGTGGTGGAATTTTTCTTTGTGCTTTGGGTAAAAGGTGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTTCGCCGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                                                                                                                            <  1:294345/227‑1 (MQ=255)
                                                                                                            aCAGCTGA‑TATTTTTTGCTGGCGCGGTTGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                                                                                                                        <  2:311040/239‑1 (MQ=255)
                                                                                                                cTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCTTTGAAACGCCAt                                                                                                                     >  2:842396/1‑238 (MQ=255)
                                                                                                                 tGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGGCGTCACCCTGTGCCGCTTTCAGTCCGGTATCGAACAAACCCTCTTTGAAAAGCCATg                                                                                                                    >  1:378492/1‑238 (MQ=255)
                                                                                                                 tGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACAGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGACTCGACCAAACCCACTTTTAAACGCCAtc                                                                                                                    >  2:825718/1‑237 (MQ=255)
                                                                                                                 tGA‑TATTTTTTGCTGGCGCGGTAGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg                                                                                                                    >  1:221345/1‑238 (MQ=255)
                                                                                                                    tttttttttGCTGGCGCGGTCGATTTTTTTTTTTGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                 <  1:429392/236‑1 (MQ=255)
                                                                                                                    tttttttttGCGGGCGCGGTCGAATTTGTTTTTGTGCTGGGGGTAAAAGTGGAAGTACCAGGAAAATTTTTTC‑GGTGACGAGGTGATCTGAATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                 <  1:873556/236‑1 (MQ=255)
                                                                                                                     tttttgttTCTGGGGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                 <  1:371212/236‑1 (MQ=255)
                                                                                                                     tatTTTTTGCTGGCGTGGTCGAATGTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                 <  2:831509/238‑1 (MQ=255)
                                                                                                                     tatTTTTTGCTGGCCCGGTCGAATTTGTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                 <  2:378492/238‑1 (MQ=255)
                                                                                                                     tatTTTTTGCTGACGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                 <  2:4400/238‑1 (MQ=255)
                                                                                                                              cTGGCGCGGTTTAATTTTTTTTTGTGCTTGGGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc                                                                                                       <  1:117144/239‑1 (MQ=255)
                                                                                                                              cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                         <  2:650918/141‑1 (MQ=255)
                                                                                                                              cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                         >  1:650918/1‑141 (MQ=255)
                                                                                                                                  cgcgGTCGAATTTGTCTTTGAGCTTGAGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGATGAGGTGCTCTTCATTTCACCGAGCTTGCTATGCTGGCTCTGCGTCGCCTCTTCTCATTTCCACGGTTCGCGCTACTCCACCCACATCCCCAGGCCGATATGGTATTCCTCTCCCGCTTTCAGCTCGGCATCGACCAACAACCCTTTACAACGccccgccaaagcaaaggcaagg                                                                                                   >  1:442180/1‑219 (MQ=255)
                                                                                                                                   ggcgTCGAAATTGTCGTTTGGCTTGCGGTAAAAGTTGAAATACCCGGAGAATTTTTTC‑GGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg                                                                                                   <  2:492579/235‑1 (MQ=255)
                                                                                                                                   gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg                                                                                                   <  2:259818/238‑1 (MQ=255)
                                                                                                                                     ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt                                                                                                 <  2:221345/238‑1 (MQ=255)
                                                                                                                                        cgtaTTTTTCTTTGTGATTGCGGAAAAAGTGAAAGTCCCCGGAGAAGTTTTTC‑GGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTATTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCa                                                                                             <  1:474092/236‑1 (MQ=255)
                                                                                                                                         gAATTTTTTTTTGTGCTGGCGTTAAAAGTGTACGGCCACGGAGAATTTTTTCGGGTGCCG‑GGTGACCTTCATTTCCCGTTGATTGATATGCAGGCTCTGCTTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGTTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGCCATGCCCAGGTAAAGTCCTGGTTGACaa                                                                                            <  1:593393/239‑1 (MQ=255)
                                                                                                                                                 cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTCAAGACCTGGTTGCCAATGATATTg                                                                                    >  2:721370/1‑239 (MQ=255)
                                                                                                                                                    tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                                                                                                                            <  2:699960/196‑1 (MQ=255)
                                                                                                                                                    tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                                                                                                                            >  1:699960/1‑196 (MQ=255)
                                                                                                                                                          tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGTTCTGCATTTCACCGTGATTGATATGCAGGTTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTTTGCCGCGTTCAGTTCGGTATCGAACAACCACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTTTAg                                                                           >  2:277522/1‑239 (MQ=255)
                                                                                                                                                          tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTTTGCCGCTTTCAGTTCGGTATCGAACAAACACCCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTCCCAATGAAATGGGGttgggaa                                                                           >  1:458202/1‑234 (MQ=255)
                                                                                                                                                                      ggAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGt                                                               <  2:243388/239‑1 (MQ=255)
                                                                                                                                                                       gAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAACCGCCATGCCCCGGTAAAGACCTGGTTGCCAATGCTATTTCGTTTGTAGAACAggcgggcgg                                                              >  2:719230/1‑235 (MQ=255)
                                                                                                                                                                       gAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTg                                                              >  2:75661/1‑239 (MQ=255)
                                                                                                                                                                             cccGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTTCGTTTGTAGAACAGGCGGCGGGAATACg                                                        >  2:645740/1‑239 (MQ=255)
                                                                                                                                                                              ccggtaaaTTTTTTC‑GGGGGCGGGGTGATCTGCATTTCCCCGTGTTTTATATGCAGGCTCTCTGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTTTCGAACAAACTCTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACgg                                                       <  1:547574/233‑1 (MQ=255)
                                                                                                                                                                                  agaATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAAAACAGGCGGCGTGaaaaggggggt                                                   >  1:331566/1‑230 (MQ=255)
                                                                                                                                                                                           ttcggtggcgggggtttttGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATa                                           <  1:75661/221‑1 (MQ=255)
                                                                                                                                                                                               gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGACGGCGTGAATACGGCGAATTGGGAAAAAGc                                       >  1:242309/1‑239 (MQ=255)
                                                                                                                                                                                                 gaggAGGTGATCTGCATCTCCCCTTTATTGATTTGCAGGCTCTGCGTCGCCTTTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATGCGCAGGTAGAATTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTTAATACGGCGATTTGGGATACAGCgg                                     <  1:572804/236‑1 (MQ=255)
                                                                                                                                                                                                          aTCTGCATTTCACCGTGATTGATTTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGc                            <  2:420064/239‑1 (MQ=255)
                                                                                                                                                                                                            cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCg                          >  2:873442/1‑239 (MQ=255)
                                                                                                                                                                                                              gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGt                        <  2:636184/239‑1 (MQ=255)
                                                                                                                                                                                                               taTTTCCCCGTGATTGATATGCGGGCTCTGCGTCGCCTGTTCTACTTTCCCAGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGGCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGt                        <  2:570328/237‑1 (MQ=255)
                                                                                                                                                                                                                  ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGTTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGCCAAGCTTCCCGGTTggg                    >  1:17680/1‑239 (MQ=255)
                                                                                                                                                                                                                    ccccGTTATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAAGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGaa                  <  2:564699/237‑1 (MQ=255)
                                                                                                                                                                                                                    cACCGTGATTGATATGGAGGCTCTGCGTCGCCTTTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGTCCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGaa                  <  2:56605/239‑1 (MQ=255)
                                                                                                                                                                                                                    cACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGaa                  >  2:775243/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGGTTTGGGATACATCGGCAAGCTTGCCGGTTGGGAATa                >  2:806761/1‑238 (MQ=255)
                                                                                                                                                                                                                       cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTCCCGGTTGGGGATAg               >  2:18323/1‑239 (MQ=255)
                                                                                                                                                                                                                         tgattgatATGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGGGAGTACGGGGATTTTGGGTACAGCGGACGACTTGCCGGGTgggggaaggc             >  1:570328/1‑232 (MQ=255)
                                                                                                                                                                                                                         tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCTGCTTTCAGTTCGGTCTCGACCAAACACTCTTTGAAACGCCATCCCCAGGTAAAGACCTGGTTGCAAATGTTATTGCGTTTGTAGAACAGCGGGCGGGCATACGGCGCTTTGGGATCCAGCGGCAAGTTTGCCGGTTGGGAATaaaa             >  1:616939/1‑236 (MQ=255)
                                                                                                                                                                                                                         tgattgatATGCAGGCTCTGCGTCGACTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAAACCTGGTTGCCATCGATATTGAGTTTGTAGAAAAGGGCGCCGGAATACGCGCATTTGGGTTACCAAGGAAAGCCTGGCGGATGGGAAAAg               >  1:211958/1‑237 (MQ=255)
                                                                                                                                                                                                                           attgattgGCAGGCCCGGCGTCCCCTTTTCTACCTTCCCCGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACt            <  1:35759/238‑1 (MQ=255)
                                                                                                                                                                                                                               atatGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGcc       <  2:331566/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGc  >  2:35759/1‑238 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
TTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGA‑TATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTC‑GGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGC  >  NC_000913/2132557‑2133021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A