Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F113 I1 R1	10	64.6	2245352	94.4%	2119612	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	111.0 / NA	40	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (23/17); total (23/17)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925

ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTc                                                                                                                                          >  2:579009/1‑143 (MQ=255)

ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGACTGATATGCAGGCTc                                                                                                                                          >  1:466960/1‑143 (MQ=255)

  aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCACTTCACCGTGATTCATATGCAGGCTCTg                                                                                                                                        >  1:107721/1‑143 (MQ=255)

          gagTGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAAGTTGTCTTTGTGGTTGCGGTAGAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct                                                                                                                                <  1:127637/143‑1 (MQ=255)

                taaCAGCTGGTATTTGTTGCTGGCGGGGTTGGATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGGATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                          <  1:744042/142‑1 (MQ=255)

                  aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                        >  2:748160/1‑143 (MQ=255)

                       tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:864300/143‑1 (MQ=255)

                        gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGTCTAAGCGTCGCCTGTTCTACttgacac                                                                                                                  >  2:718673/1‑138 (MQ=255)

                            ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTCCCACTTTCCACGGtt                                                                                                              >  1:597520/1‑143 (MQ=255)

                                   cTGGCGCGGACGAATTTGCCTTTGGGCTTGCGGTAAACGTGGTTGTACCTGGAGAATTTTTTCGGTGACGAACTTACCTCCATTTTTCCGTGATTGATATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTa                                                                                                       <  1:446381/143‑1 (MQ=255)

                                    tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCCGGCCCGGCGCCGCCTCTTCTACTTTCCACGGTTCCCCGtcc                                                                                                      >  2:37769/1‑141 (MQ=255)

                                             cGAGTTTGTCTTTGTGCTTGGGGTGAAAGTGGAAGTAGCCGGCGAATTCCTTCCGTGACGAGGTGATCGGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc                                                                                             <  1:230818/143‑1 (MQ=255)

                                              gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTACTccaccacca                                                                                            >  2:460847/1‑143 (MQ=255)

                                                 tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCAtgc                                                                                         >  2:1091/1‑141 (MQ=255)

                                                    gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                      >  2:1097874/1‑143 (MQ=255)

                                                    gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTCTCCACGGTTTGACGTAGTccacgaccgccggcc                                                                                      >  1:3271/1‑136 (MQ=255)

                                                      cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACCCCACCACCATCCGCCgg                                                                                    >  1:317558/1‑143 (MQ=255)

                                                      cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCCGCGTCGCCTCTTCTACTTTTCACGGTTCTCCGTACTTCTCTAGCATCCGCAgg                                                                                    >  2:973056/1‑143 (MQ=255)

                                                               tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACGGTTCGCCAGACTCGACCACCAGACGCCGGAAGATCTCg                                                                           >  1:325305/1‑143 (MQ=255)

                                                                  tcggacagcggACGTCCCCGGACAATTTTTGCTGTGACGACGTGATCTTCATTTCACCGTGATGGATATGCAGGCTTTGCCTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                        <  1:280262/134‑1 (MQ=255)

                                                                    tagatgtggccgTACCCGGAGAATGTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                      <  1:357638/132‑1 (MQ=255)

                                                                        aGTCGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                  <  2:391979/143‑1 (MQ=255)

                                                                                  cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                        <  2:994350/143‑1 (MQ=255)

                                                                                   ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCAGATGTCGTAATCCTGTGCCGCTTTCAg                                                       >  1:277741/1‑143 (MQ=255)

                                                                                       agaATTTTTTCGGTTACGACGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                   <  2:439503/143‑1 (MQ=255)

                                                                                         gattgcttCGGTGACGCGGTGATCTGCATTTCACCGTTCTTGATATGCAGCCTCTGCGTCGCCTCTTCTACTTCCCACGGTTCGCCGTACTCCACCACCATCCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt                                                 <  1:717556/137‑1 (MQ=255)

                                                                                           ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCCGATGTCGTAATGCTGTGCCGCTTTCAGTTCGGTac                                               >  1:900849/1‑142 (MQ=255)

                                                                                            tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTGCAGTTCGGTATc                                              >  2:40184/1‑143 (MQ=255)

                                                                                             tttacgggctcGATGTGATCTGCATTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACCGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                             <  1:959400/133‑1 (MQ=255)

                                                                                                  ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTACTTTCCACGGTTCCACATACTCCACCACCATCCCCAGGAAGCTGTCGTAACCCTGTGCCGCCTTCAGTACGTCAGCGaacac                                        >  1:128910/1‑142 (MQ=255)

                                                                                                   gttaCGCGGTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCATCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:818556/140‑1 (MQ=255)

                                                                                                   gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGGCGTACTCCACCACCAACCGCAGGAAGATGAGGTAATCCTTTGCATATTGCAATTCGGTGTAGaacaaa                                       >  1:44882/1‑143 (MQ=255)

                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGACGGCATTGTTTGCCGCTTTCAGTTCGATAGCGaacaaacaacc                                  >  1:600236/1‑140 (MQ=255)

                                                                                                          ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCACTTTCAGTTCGGTATCGCACAAACACTCtt                                >  2:599322/1‑143 (MQ=255)

                                                                                                             gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                             <  2:912401/143‑1 (MQ=255)

                                                                                                                         aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCAACCGCAGGCACCCGTCGTAATCCTGTGCCGCTCTCACTCCGGTGTCGGACCCCCCCTCTTTGACGCGCCATcccc                 >  1:445519/1‑143 (MQ=255)

                                                                                                                           cGTGATTGCTACGCAGGCGCTGCGGCGCCTCTTCTACTTTCCACGGTTGGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg               <  1:214572/143‑1 (MQ=255)

                                                                                                                           cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCTCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCCTTGAAACGCCCTGCCCAg               >  2:195881/1‑143 (MQ=255)

                                                                                                                                   ctatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc       <  2:385094/142‑1 (MQ=255)

                                                                                                                                        cAGGCTCTGCGTCGCCTCCTCGACTTGCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt  <  1:763177/143‑1 (MQ=255)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A