Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F108 I2 R1	13	72.2	2506112	94.4%	2365769	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	146.6 / NA	53	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (29/24); total (29/24)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132650‑2132919

tCTGGTAGAGGGTAAACAGCTGACATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                 <  1:1098290/143‑1 (MQ=255)

tCTGGTAGAGGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACATGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                 >  2:1040435/1‑143 (MQ=255)

  tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATACGCAGGCTCTGCGt                                                                                                                               <  2:324941/143‑1 (MQ=255)

   gggagagGGTGAACAGCTGATATTTTCGGCGGGCGCGGTCGAATTGGCCTTTGTGCTTGCGGGAAAAGTGGATGGACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                              <  2:392463/140‑1 (MQ=255)

     tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                            <  2:710625/143‑1 (MQ=255)

       gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCCTGATTGATACGCCGGCTCTGCGTCGCct                                                                                                                          >  2:970328/1‑143 (MQ=255)

            tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGGGTCGcttcttat                                                                                                                     >  1:282798/1‑141 (MQ=255)

              aaCAGCTGATATTTTTTGCTGGCGCGATCGAATTTGTCTTTGCGCTTGCGGTAAAAGTGGAAGTGCCGGGAGTAGTTTTTCGGTGCCGAGGTGATCTGACTTTCACCGTGATTGATATACAAGCTCTGGGTCGCCtcctctcc                                                                                                                   >  1:606188/1‑141 (MQ=255)

               aCAGCTGATATTTTTTGCAGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACAGTGATTGCTATGTAGGCTCTGCGTCGCGTCTTGTAcg                                                                                                                  >  1:136536/1‑142 (MQ=255)

                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCGTTTCACGGTGATTGATATGCCGGCTCTGCGTGGACTCTCCTACTTGccg                                                                                                             >  2:842306/1‑142 (MQ=255)

                        atTTTTTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt                                                                                                         <  1:825149/143‑1 (MQ=255)

                         ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACCGtt                                                                                                        >  1:1232396/1‑143 (MQ=255)

                         ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                        >  2:1049194/1‑143 (MQ=255)

                         ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                        >  2:1036263/1‑143 (MQ=255)

                          tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCGTCTTCTACTTTCCACGGTTc                                                                                                       >  1:1036406/1‑143 (MQ=255)

                             ttGCTGGCGCGGTTGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                    <  2:277381/143‑1 (MQ=255)

                               gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCCGTAAAAGTGGAAGTACCCAGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGccct                                                                                                  >  1:656495/1‑141 (MQ=255)

                                      gggTCGCATGTGTCGTTGGGTGTGCGGTAAAGGGGGACGTAGCCGGAGAATGTTTGAGGTGGCGAGGTGGTGTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                           <  1:640341/142‑1 (MQ=255)

                                            agttgttCTTTGGGCGTGCGGTAGAAGTGGACGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTTCGCCGTACTCCACCACCAt                                                                                     <  1:52397/137‑1 (MQ=255)

                                                     ttttgCTTGCGTTAAAAGCGCAGGTACTCGGAGAATTGTTTCGGTGACGAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                            <  1:1105822/140‑1 (MQ=255)

                                                          cTTGCGTGAAAAGTTGACGTACCCGGTGCATCTTTTCAGTGAGGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                       <  1:1209183/143‑1 (MQ=255)

                                                           ttGCGGTAAACGTGGACGTCCCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                      <  1:1117362/143‑1 (MQ=255)

                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGCAAGATGTCGTaa                                                                  >  2:608952/1‑143 (MQ=255)

                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTac                                                                  >  2:419241/1‑142 (MQ=255)

                                                                 tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATc                                                                >  1:272313/1‑143 (MQ=255)

                                                                  aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTGCTCCACCGCCATCCGCAGGAAGCTGTCGtgagtc                                                               >  2:588930/1‑138 (MQ=255)

                                                                      gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGCTGGCTTGTGATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                           <  2:285379/143‑1 (MQ=255)

                                                                         gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCACTTCACCGTGATTGATATGCAGGCTCACCGTCGCCTCTTCTCCTCTCCACGGGTCGCCGGACTCGAACACCATCCGCAAGAAGGTGTCGTacaacggtgccc                                                        >  1:157849/1‑132 (MQ=255)

                                                                                 cGGAGAATTTTTTCACTGACGAGCTGCTGTGCTGCGCACCGCGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTCTGCCGCTTTCAGt                                                >  1:271049/1‑143 (MQ=255)

                                                                                  ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACTACCATCCGCAGGAAGATGTCGTAATCCTGTGCAGCTTTCAGtt                                               >  2:942202/1‑143 (MQ=255)

                                                                                   gagaATTTTTTCGGTGAGGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGTCACCTCTTGTACTTTCCACGGTTCGCCGTACTCCACCACCGTGCGCCTGCAGATGTCGTAAGCCTGTGGGGCTGTCAGTTc                                              >  2:600059/1‑143 (MQ=255)

                                                                                    aggtttttttCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                             <  1:786485/139‑1 (MQ=255)

                                                                                         tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                        <  2:524311/143‑1 (MQ=255)

                                                                                         tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTTCCGCTTTCAGTTCGTTATc                                        >  1:94914/1‑143 (MQ=255)

                                                                                         tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                        <  2:573472/143‑1 (MQ=255)

                                                                                              cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGgaca                                   >  2:388779/1‑143 (MQ=255)

                                                                                                   gcggggTGATGTGCATTTCACCGTGGTTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac                              <  1:539822/139‑1 (MQ=255)

                                                                                                      aGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGGCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct                           <  2:57709/143‑1 (MQ=255)

                                                                                                        gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                         <  2:346900/143‑1 (MQ=255)

                                                                                                           aTCTGCATTTCACCGTGATTGATATGCAGTCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAGTCCTCTGCCGCTTTCAGTTCGGTAAACAACAAACGctctgcgaa                      >  1:834693/1‑138 (MQ=255)

                                                                                                           aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                      >  2:637334/1‑143 (MQ=255)

                                                                                                            tCTGCATTTCCTCGCGAGTGATCTGCAGGCTCTGCGTCCCCTCTTCTACTTTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                     <  1:552142/143‑1 (MQ=255)

                                                                                                            tCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGaaa                     <  1:482381/143‑1 (MQ=255)

                                                                                                             cttctgttCACCGAGATTACTATGCATGCTCTGCGTCTCCTCTCCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCTTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                    <  1:900609/137‑1 (MQ=255)

                                                                                                              tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCCGGTCGGTACCGAACAAACAGTCCTTgcacca                   >  1:33500/1‑138 (MQ=255)

                                                                                                               gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAAccc                  >  2:213431/1‑141 (MQ=255)

                                                                                                                      aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTCGGTATCGAACCAACACTCTTTGAAACACCATGccc           >  2:5991/1‑143 (MQ=255)

                                                                                                                       ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa          <  1:198922/143‑1 (MQ=255)

                                                                                                                        cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg         >  1:250549/1‑143 (MQ=255)

                                                                                                                         gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg        >  2:721976/1‑143 (MQ=255)

                                                                                                                             ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACCCTTTGAAACGCCATGCCCAGGTaaa    >  1:163432/1‑143 (MQ=255)

                                                                                                                              tgatAGGCAGGCTCTGCTTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg   <  1:787765/143‑1 (MQ=255)

                                                                                                                               gatATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa  <  2:735089/143‑1 (MQ=255)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132650‑2132919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A