Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A25 F36 I1 R1 8 171.3 3462537 90.8% 3143983 257.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG99.3% 516.9 / NA 143L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (79/63);  minor base A (1/0);  total (80/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGAC  >  NC_000913/2317820‑2318367
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                
   gTAAATTGGCTTACAATTCCCGGTTCTGAATCTAACGAGATAACGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTACCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                <  1:177511/275‑1 (MQ=255)
   gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                <  1:116781/275‑1 (MQ=255)
   gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                <  1:523056/275‑1 (MQ=255)
     aaaCTGGCTGCCCATTCCCGTTTCTGAATCTACCGATATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                           <  2:280025/278‑1 (MQ=255)
        cTGGCTGCCCATTCCCGGTTCTGAATCTACCGGGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg                                                                                                                                                                                                                                                                            <  1:3954/274‑1 (MQ=255)
              gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTACACAGCCGGTATCGGTGAATTTGATGGCGTTACTGAACAGGTTGGAGATGa                                                                                                                                                                                                                                                                  >  2:456879/1‑278 (MQ=255)
                        ggTTCTGAATCTACCGAGAAATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTa                                                                                                                                                                                                                                                         <  2:621896/277‑1 (MQ=255)
                               aaTCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGtt                                                                                                                                                                                                                                                                          >  1:727712/1‑253 (MQ=255)
                                   tACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgag                                                                                                                                                                                                                                                                      <  3:727711/253‑1 (MQ=255)
                                    aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATCCAGCCGGTATCGGTGACTTTTATGGCTTTACTGAACAGGTTTGAGATGACCTGCTGTAAACGccaaggggc                                                                                                                                                                                                                                            >  4:126941/1‑270 (MQ=255)
                                      cGAGATATCCCCCGCCATCATGCGGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg                                                                                                                                                                                                                                           <  4:324884/277‑1 (MQ=255)
                                             tCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCTATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTa                                                                                                                                                                                                                                    >  2:536843/1‑277 (MQ=255)
                                              cGCCGTCCATCATGCTTAGCAGTCCGTCCAAGATCGCCCTACCCAGACCGGACCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGAAAACAAACAAGCGCACCACTTCTTTCGCCGGTATCCCACCGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                                                                                                                                                                  <  4:782238/278‑1 (MQ=255)
                                                  gTCCATCCTGCTGACCAGTTCTTCAAAACTCGCCAGACCCAGACCGGTCCCCTGGAAAGTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                              <  2:766789/278‑1 (MQ=255)
                                                  gTCCATCATGCTGATCAGTTTCTCACAACTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                              <  2:641152/278‑1 (MQ=255)
                                                  gTCCATCATGCTAATCAGTTTTTAACAACTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                              <  2:482225/278‑1 (MQ=255)
                                                          tGCTGATCAGTTTTTCGCCAGTCCCCAGACCCAGGCCGTTGCCCTGGAAACTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGGTCAAACAAGCGCCCCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGGTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca                                                                                                                                                                                                                      <  2:652697/278‑1 (MQ=255)
                                                               ataagttatccccaAAACGCCAGCCCCAGCCCGGTCCCCTGGAAATTACGCGGTACGCCCGTTCCGACCTGAAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTAAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  2:105230/266‑1 (MQ=255)
                                                               aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTCTCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTAACCAGCCGGTACAGGTGAATTTTATGGCGTCTATGAACAGGTTGGAGCTGACCTGCTGTAACTGCACCGGGTGGCCATTTACGGCCACTGGCCCATc                                                                                                                                                                                                                    >  3:178346/1‑275 (MQ=255)
                                                               aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACCTCCg                                                                                                                                                                                                                  >  2:799156/1‑277 (MQ=255)
                                                               aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                    >  1:306386/1‑275 (MQ=255)
                                                               aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCAATGGCACATc                                                                                                                                                                                                                    >  1:155878/1‑275 (MQ=255)
                                                                  aGTTTGTCACAAATGGGCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTAGCCCCGTGCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACGTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAGTCGCCATCCGCGCGAACATGCAAAACGATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  3:901297/275‑1 (MQ=255)
                                                                      tttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTTTCGGTGAAATTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCCTTTAAGGCCACTGGCCCATCCGTTTCAATa                                                                                                                                                                                                          >  2:536993/1‑278 (MQ=255)
                                                                                   cagaccccgaccGGTCCCCAGGAAATTACGCTCTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                                 <  3:381592/274‑1 (MQ=255)
                                                                                                 tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGCTAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTATACGCATCGGGTCGCCATTTAAGGCAACTGGCACCTCCGGTTCAATAAAGCAGTCCAAACCCAACTgtctg                                                                                                                                                                                  >  3:349600/1‑271 (MQ=255)
                                                                                                 tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACCGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTCAAAGCCTAACTGCTTg                                                                                                                                                                                  >  1:353093/1‑275 (MQ=255)
                                                                                                      tGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGGATAAGCCTAACTGATTGCGTac                                                                                                                                                                             >  1:529565/1‑275 (MQ=255)
                                                                                                           aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGTCGGTACCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCGGTAAACGCATCGGCTCGCCATTTACGGACACTGGCACAGTCGGTTAATTAAAGCAGTACAATCCTAACTGCTTGAGTAccccca                                                                                                                                                                        >  3:458570/1‑275 (MQ=255)
                                                                                                             tACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATCGAGAGA‑TAATCGCCATCCGCGCGAACCTGCAACACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGGTGGAGATGACCTTCTGTAAACGCATCGGGTTCGCATTTAAGGCCACTGGCCCATCCGGTTCAATAACTCCGTCCAAGCCTACCAGCTTGCCTTCCACCAGCGGt                                                                                                                                                                   >  4:346326/1‑278 (MQ=255)
                                                                                                               cGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAACCAGTACCAGCCTAACGGCTTGCTTACCACCAGCgg                                                                                                                                                                    >  3:657776/1‑275 (MQ=255)
                                                                                                               cGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTATTTCGCCGGTATCCCCACGCCGCTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATTACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCTACTTGCCCATCCGGCTCAATAAACCAGTACAAGCCTCAACGCTTGCGTACCACCAGCGTTaa                                                                                                                                                                 >  2:396895/1‑278 (MQ=255)
                                                                                                                    tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAt                                                                                                                                                               >  1:627591/1‑275 (MQ=255)
                                                                                                                    tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAt                                                                                                                                                               >  1:480660/1‑275 (MQ=255)
                                                                                                                     aCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                            <  2:126941/277‑1 (MQ=255)
                                                                                                                        cccGTGCCGAACTGGAAGAAGGGATCAAACAAGAGAACCACTTATTTCGCCGGTAGCCCCAAGCCGGTATCGCGAACACGGATAGGGAGA‑TAATCGACATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                            <  1:810243/274‑1 (MQ=255)
                                                                                                                          cGTTCCGACCTGGAAGAAGGTATCAAACAAGCGCACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTgg                                                                                                                                                         <  3:127109/275‑1 (MQ=255)
                                                                                                                              ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTCTGGCGTTACTGAACAGGTTGGAGATGACCTGCTTTATACGCTTCGGGTCGCGATTTAAGGCCACTGGCACATCCGTCTCATTAAAGCAGTCCAAGCCTCACTGCTTGAGTACTACCAGCGGTAAATAGTTGGCGGt                                                                                                                                                     >  3:392258/1‑275 (MQ=255)
                                                                                                                                  ccTGTAAAAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTACCCCCCCGGCGGTATCGCGAACACTGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                                 <  1:321318/275‑1 (MQ=255)
                                                                                                                                  ccTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  1:492510/211‑1 (MQ=255)
                                                                                                                                   cTGGAAAAAGCGATCAAACAAGCGCACCCCTTCTTTCCCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                                 <  1:676042/274‑1 (MQ=255)
                                                                                                                                      gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTTCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGt                                                                                                                                             >  1:582178/1‑275 (MQ=255)
                                                                                                                                         gaagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtca                                                                                                                                          <  1:144383/275‑1 (MQ=255)
                                                                                                                                         gaagGGATCAAACAAACGCCCCACTTACTTCGCCGGTATCCCCCAGCCGGTATCGGGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCCAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATAACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtca                                                                                                                                          <  1:435931/275‑1 (MQ=255)
                                                                                                                                                tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTACACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTCCCACCAGCGGTAAATCGTTGTCGGTGATGTGGTTCCTCACTTCAc                                                                                                                                 >  4:209279/1‑277 (MQ=255)
                                                                                                                                                        gcgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATaaa                                                                                                                                                                                                        >  2:403829/1‑198 (MQ=255)
                                                                                                                                                         cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGACCACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTACCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCCTTTAAGGCCACTGACACATACGGTTCAATAAAGCAGTACAAGCCTAACTGCTTTCGTACCACCAGCGGTAAATAGTTGGCGGTGTTGTGGTTCCTCACTTCAcgcaggtaaaa                                                                                                                       >  4:811986/1‑270 (MQ=255)
                                                                                                                                                         cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCTCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTCAAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTAATCCCTTCAAGCggcgcaa                                                                                                                        >  2:610078/1‑272 (MQ=255)
                                                                                                                                                         cgcACCACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  4:403829/198‑1 (MQ=255)
                                                                                                                                                                   cttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCAGCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGGTGGCGGTGGTGTGGGTAATCACTTAAAGCGGGGAAAACTAACGCGGt                                                                                                             >  4:399586/1‑278 (MQ=255)
                                                                                                                                                                   cttTCGCCGGTATCCCCACGCCGGTATCGCCAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACCTGCAAAACTTTACAGCCGGTATCGGTGAATTTTATGGCGTCACTGAACAGGATGGACATGACCTCCTGTAAACGCATCGGGTCGCCATTTAAGGCCACCGGCACATCCGGTTCAATAAAGTAGGAAAAGCATAACGGCTTGGCTCACACCACCGGAAAATAGTTTGTGGTGGTCAGGGTCATCACTTCACGCggctcaaaaccacg                                                                                                                 >  3:793906/1‑262 (MQ=255)
                                                                                                                                                                      tCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTCAACGCATCGGGTCGCCATTTAAGGCCACTGGCACCTCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGTTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACCCGGTGTACACTCACGCGGTTct                                                                                                          >  4:695390/1‑277 (MQ=255)
                                                                                                                                                                       ggccgtcaTCCCCCCCCCGGTTTCGCGAACACTGA‑AAAAAGACTAATCGCCATCCGCGCAAACATTCAAAACTTTACCGCCGGTATCGTTGAATTTTATGGCGTTAATGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                            <  3:480659/268‑1 (MQ=255)
                                                                                                                                                                             tATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAACTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCAACAGCGGTAAATAGTTGCCGGTTATTTGGTTCATCCCTTCACGCGGTGAAAATCCACCCGGTTCGATCt                                                                                                      >  3:422364/1‑275 (MQ=255)
                                                                                                                                                                                 cccACGCCGGTAGCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCaa                                                                                                  <  1:152601/275‑1 (MQ=255)
                                                                                                                                                                                    aCGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGCTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAACTAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGCAAACTCACGCGGTGCGATCTTCAACTGTTc                                                                                            >  2:627990/1‑278 (MQ=255)
                                                                                                                                                                                     agCCAGTATCGCGAACACGGAATGAGAGA‑TAATCGCCATCCGGGCGAACATGCAAAACTATACAGCCGTTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCGGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCaa                                                                                                  <  3:430876/270‑1 (MQ=255)
                                                                                                                                                                                           tATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTTCCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCa                                                                                     >  2:750781/1‑278 (MQ=255)
                                                                                                                                                                                                cgAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                                                                                                                                                             >  2:720100/1‑121 (MQ=255)
                                                                                                                                                                                                cgAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                                 >  4:68497/1‑213 (MQ=255)
                                                                                                                                                                                                cgAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                                 <  2:68497/213‑1 (MQ=255)
                                                                                                                                                                                                cgAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGACCACTCACGCGGTTAGATCGTCAATTGTCCCGATGCAATCtt                                                                                >  2:45885/1‑278 (MQ=255)
                                                                                                                                                                                                cgAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAAt                                                                                   >  3:19527/1‑275 (MQ=255)
                                                                                                                                                                                                  aaCACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAGCGGTGAATTTTATGGCGTTACTGAACAGGTTGTAGATGAGCTGCTGTAAACGCAACGGGTCGCCATTTCAGTCTACTGGCACATCCGTTTCAATAAAGCAGTCCAAGCCTACCTGCTTGCGTACCACCAGCGGTTTATAGCTGTCGGTGATGTGGGTCATCACTTCACGCGTTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCAATTCActct                                                                                 >  3:246099/1‑275 (MQ=255)
                                                                                                                                                                                                    cacGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTc                                                                               >  1:821136/1‑275 (MQ=255)
                                                                                                                                                                                                             agaga‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCgaga                                                                   >  2:510041/1‑278 (MQ=255)
                                                                                                                                                                                                               aga‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGCTCTTCCACTGTTCCGATTCAATCTTCGATAAATCcag                                                                    >  3:299979/1‑275 (MQ=255)
                                                                                                                                                                                                                 a‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                  >  1:245056/1‑275 (MQ=255)
                                                                                                                                                                                                                 a‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                  >  1:463026/1‑275 (MQ=255)
                                                                                                                                                                                                                 a‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                  >  1:219754/1‑275 (MQ=255)
                                                                                                                                                                                                                     aTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTGCCGATTCAATCTTCGAGAAATCGAGAATATCGc                                                            >  2:685265/1‑278 (MQ=255)
                                                                                                                                                                                                                        gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGATTGCGTACCACCAGCGGTAACTAGTTGGGGGTGATGTGGGTCATCACTTCACGCGGTGAAAACTCACGCGGTTAGATCTTCAACTGTTCCGATTCAATCTTCCAGAAATAGAGAATATCGCTGa                                                         >  2:32444/1‑278 (MQ=255)
                                                                                                                                                                                                                             ccGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCCGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGGGATGTGGTTCATCACTTCACGCGGGGAAAACCCACGCGGTTCGATCTTCAACTGTTCCTATTCAATCTTACAGAAATCGAGAATATCGCtaaca                                                       >  3:362101/1‑271 (MQ=255)
                                                                                                                                                                                                                                cgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATa                                                       >  1:277496/1‑272 (MQ=255)
                                                                                                                                                                                                                                cgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTAAATCTTCGAGAAATCGAGAATATCGCCGATAAtt                                                    >  2:182002/1‑275 (MQ=255)
                                                                                                                                                                                                                                        tGCAAAACTCTACAGCCGGTATCGGGGAATCTTATGGCGGTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCTGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAg                                         <  4:404693/278‑1 (MQ=255)
                                                                                                                                                                                                                                           aaaaCTATACAGCCGGTATCGGTGAATTGTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAg                                         <  1:422365/275‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCTATGCGGCCGGTATCGGTGAATTTTATGGCGTGACTGAACAGGTTGGAGATGACCTGCTGTAAAGGCATCGGATCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCGGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGc                                       <  3:248053/275‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCTATACAGCCAGTATCGGTGAATTTTATGGCGTTACTGACCAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGAGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGc                                       <  1:458571/275‑1 (MQ=255)
                                                                                                                                                                                                                                                 ataacgCCGGTAGCGGTGAATTTTATGGCGTTACGGGACAGGTTGGAGATGACCTGCTGGAAACGCATCGTGTCGCCATTTAAGGCCACTGGCACATCCGGTTAAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAAGTTTCAACAACAGGCTgg                                    <  3:493224/269‑1 (MQ=255)
                                                                                                                                                                                                                                                 ataCAGCCGGGATCGGGGAATTTTATGGCGTTACGGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTAGCCATTTAAGGCCACTGGCACATCCGGTTCAAGAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                    <  3:15531/274‑1 (MQ=255)
                                                                                                                                                                                                                                                  taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAt                                                                                        >  4:48233/1‑221 (MQ=255)
                                                                                                                                                                                                                                                  taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACCTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGTGGATATTTTTCAAAAAAAGGCTGGa                                   >  3:667805/1‑274 (MQ=255)
                                                                                                                                                                                                                                                   aCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAtt                                                                                       <  2:48233/221‑1 (MQ=255)
                                                                                                                                                                                                                                                       ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTTCGATTCAATCTTCGCGCAATCGAGAATATCGCTTGTAATTTTCAAACACCGGTCGGAagatt                              >  1:358759/1‑272 (MQ=255)
                                                                                                                                                                                                                                                       ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgt                              >  1:776942/1‑274 (MQ=255)
                                                                                                                                                                                                                                                         ggTATCGGTGAATTTTATGGCGTTCCTGAACAGGTTGGGGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCa                        <  2:29194/278‑1 (MQ=255)
                                                                                                                                                                                                                                                         ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgt                           >  1:591453/1‑275 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGcc                                                                                                                                                                                                                                         >  1:752878/1‑68 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCACCAACAGGCTGGAAGAgttgtt                          >  3:893344/1‑275 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgt                           >  3:724043/1‑274 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAAAAGGCTGGAAGAgttttt                          >  3:655110/1‑275 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGTGAATTTTATGGCCTTACTGAACAGGTTGGAGATGACCTGCTGGAAACCCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgtt                          <  1:657777/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            gtttgtaaaTTTTATGGGCTTACAGAACAGGTGCGAGATTACCTGCTGTAAACGCATCGGGTCGTCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAATCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCa                        <  3:46508/268‑1 (MQ=255)
                                                                                                                                                                                                                                                               ggTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGt                                                                                                             >  4:46648/1‑187 (MQ=255)
                                                                                                                                                                                                                                                                 tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGc                    >  3:520007/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                     ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAgg                                        >  3:754091/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                               ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTa                                                                                                                                                                                                  >  2:181357/1‑86 (MQ=255)
                                                                                                                                                                                                                                                                               ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGAc  >  2:740942/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                tACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                                 <  4:181357/86‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                
ACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGAC  >  NC_000913/2317820‑2318367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A