Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F106 I1 R1 11 134.4 2836798 90.9% 2578649 259.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG98.0% 351.2 / NA 101L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (43/56);  minor base T (2/0);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCC  >  NC_000913/2317822‑2318360
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                         
gttAAACTGGCTGCCCATTCCCGGTCCTGAATCTACAGAGCTAACGCCGTCCATCCTGCTGGTCAGTGTTTCACAGATCGACAGACCCAGACCGGTCCCCTGGAGATGACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                         <  4:201457/274‑1 (MQ=255)
ggTAACCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                         <  2:392632/276‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                         <  3:469165/275‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGGCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCTGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                         <  1:573733/275‑1 (MQ=255)
 gTAAACTGACTGCCCATGCCCGGTTCTAAATCTACCGAGATATCAGCGTCCATCATGCTGATCAGTTATTCACAAATCGCCAGACCCAGACCTGTCCCCTGGAAATTACGCTGTACGCCCGTTACG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                         <  1:625671/275‑1 (MQ=255)
        ggCTGCCCATGCCCTGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTCTTCACAAATCGCCAGCCCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                    <  1:282752/273‑1 (MQ=255)
          cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTCACGGTGACTTTTATGGCGTTAATGAACACGTTGgag                                                                                                                                                                                                                                                               >  2:522480/1‑276 (MQ=255)
           ttcccATTCCCGGTTCTGAAACTACCGAGATGTCGCCGTCCCTCATGCTGATCATTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgag                                                                                                                                                                                                                                                               <  3:422523/273‑1 (MQ=255)
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTTGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACCTGCAAAACTATACAGCCGGTATCGGCGAATTTTATGGCGTTACTGaaacggtatgaga                                                                                                                                                                                                                                                              >  3:237171/1‑264 (MQ=255)
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCTGCCCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGTGTTACTGAAAAGGTTGgag                                                                                                                                                                                                                                                               >  1:568210/1‑274 (MQ=255)
                aatCCCGGTTCTGAATCCTCCGTGATATCGCCGTCCATCAAGCTGATCAGTTTTTCACAACTCGCCCGACCCAAACCCGTCCCCTGGAAATTCCTCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGAGCAAACAAGCGCACCACTCCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                          <  2:316473/273‑1 (MQ=255)
                 ttCCCGGTTCTGAATCTACCGGGATATCGCCGTCCATCATTCTGATCAGTTTTTCACAAATCGCCAGACCCCGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                          <  1:180027/274‑1 (MQ=255)
                 atCCCTGTTCTCAACTTACCGACAGATACCCTTCCATCATGCTGTTCAGTTTTTCCCCAATCGCCCGACCCACACCGGTCCCCTGGAAATTACGCTGTACTCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAcc                                                                                                                                                                                                                                                         <  4:676663/274‑1 (MQ=255)
                   cccGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCACACCCGCGAACATACAAAACTATACAGCCGGTATCGGAGAATTTTACGGCGTTACTTAACCGGTTGGAACTGACctt                                                                                                                                                                                                                                                       >  1:630934/1‑274 (MQ=255)
                        ttCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCTCCATCCGCGCGACCCTGCAAAACTATACAGCCGGTCTCGGTGAATTTTTTCGCGTTACTGAACAGGTTGGAGCTGAActgcttca                                                                                                                                                                                                                                                  >  2:666910/1‑272 (MQ=255)
                         tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCGGTa                                                                                                                                                                                                                                                  >  1:570139/1‑274 (MQ=255)
                                                gTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTCTACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGCGTCGCCATTTAAGGc                                                                                                                                                                                                                         >  2:224872/1‑276 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACCTGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTAATGAACAGGTTGGACATGACCTGCTGTAAACGCATCGGGTTGCCCTTTAAGGc                                                                                                                                                                                                                         >  3:329867/1‑275 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCg                                                                                                                                                                                                                                         >  1:574642/1‑259 (MQ=255)
                                                  ccATCATGCTGCTACGTGTTACGCAAACCGCCAGACCCAGCCCGGTCCCCTGGAAATTACGCGGGCCGCCCGTTCCG‑ACCTGGAAGAAGGGATCCAACAAGCGGACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                       <  4:185773/276‑1 (MQ=255)
                                                  ccATCATGCTGAGCAGTTTTGCACAAACCGCCAGACCCAGACCGGGCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                       <  2:102257/276‑1 (MQ=255)
                                                     tcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                                                                                                                                                     <  3:574642/259‑1 (MQ=255)
                                                           ttaTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca                                                                                                                                                                                                               <  1:69948/273‑1 (MQ=255)
                                                           tgatacgTTTTTCACAAATCCCCAGACCCAGACCGGTCCCCTGGAAATTGCGCTGTACGCCCGTTCCG‑ACCTGGAAGGAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca                                                                                                                                                                                                               <  1:349815/269‑1 (MQ=255)
                                                           tGATCCGTTTTGCGCAAATCGCCAGACCCAGACCAGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca                                                                                                                                                                                                               <  3:200934/275‑1 (MQ=255)
                                                             aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGACATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTAATGAACGGGTTGGAGATGACCTGCTGTAAACGCCACGGGTCGCCATTTAAGTCAACTGGCCCATc                                                                                                                                                                                                             >  3:200714/1‑275 (MQ=255)
                                                             aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATcc                                                                                                                                                                                                            >  2:189864/1‑276 (MQ=255)
                                                                aggttttCACAAATCGCCAGACCCAGACCGGGCCCCTGGAAAGTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACGATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                          <  3:713494/272‑1 (MQ=255)
                                                                         caAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                <  2:377220/276‑1 (MQ=255)
                                                                          cgaacacccgacctagcccTGTCCCCTGGAAATGACGCTCTACGCCCGTTCCGCACCT‑GCAGAAGGAATCAAACAAGCAAACCACGGCTTACGCCGGTATCCCCAAGCCGGTATCGTGAACACAGATAGAGCGATAATCGCCATCCGCAGCAACACGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGAGTTACTGAACAGGTTGGAGATGAGCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                <  3:371698/266‑1 (MQ=255)
                                                                          aaaaagCCAGACCAGGAACGGGGCCCTGGAAATTGACCTGAACGCCCGCTCCG‑ACCTGGAAGAAGGGATCAAACAAGGGCACCACTCGTGTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                <  3:689786/270‑1 (MQ=255)
                                                                          aaaTCGCCAGAACCGGCCCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                <  1:59698/275‑1 (MQ=255)
                                                                               gcacgaccccagccGGTCCCCTGGAAATTACGCAGTACGCCCGGTCCG‑AGCTGGAAGAAGGCATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGATCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                          <  4:219790/264‑1 (MQ=255)
                                                                               gCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGGTCCG‑ACCTGGAAGAGGGAATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCTGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                          <  4:201809/276‑1 (MQ=255)
                                                                                          accGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACt                                                                                                                                                                                >  2:337966/1‑275 (MQ=255)
                                                                                              gTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACCGGTTGGAGCTGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCACTAACGCAGTACAAGCCTAACTCCtt                                                                                                                                                                            >  3:158279/1‑275 (MQ=255)
                                                                                              gTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTg                                                                                                                                                                           >  2:67931/1‑276 (MQ=255)
                                                                                              gTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGAATCGGGTCGCCATTTAAGGCAACTGGCACATCCGGTTCAAGTAAGAAGTACAAGCCTAACTGATTg                                                                                                                                                                           >  4:49348/1‑276 (MQ=255)
                                                                                               tCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACGACTTCTTTCGCCGGTATCGCCACGCCGGNATCGCGAACACGGCTAGCGAGTTAATCGCCAGCCGAGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGCAGACCTGCTGTAAAAGCATAGGGTCGGCATTTAAGGGCACCGGGCCAGCCTGTTTAAGTAAATAGTACAAGCCGaaacacctg                                                                                                                                                                           >  4:382107/1‑268 (MQ=255)
                                                                                               tCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACTCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGc                                                                                                                                                                          >  2:612183/1‑276 (MQ=255)
                                                                                                    tGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTACAGGTGAATTTTATGGCGTTACTTAACAGGCTGGAGATGACCTTCTGTAAACGCATCGGGTCTCCCTTTAAGGCCACTGGCACATCCGGTTCAATAAATCAGTACAAGCCTAACTTCTTGCGTac                                                                                                                                                                      >  1:627365/1‑275 (MQ=255)
                                                                                                         agagaccggTACGCCCGTTCCT‑ACCTGGAAGAAGGGATCAAACAAGCGCCCCACTTCTTTCGCCTGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGAGAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                   <  3:405682/265‑1 (MQ=255)
                                                                                                         aTTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                 <  1:119110/275‑1 (MQ=255)
                                                                                                         aTTACGCGGTAAGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                   <  1:329867/273‑1 (MQ=255)
                                                                                                          ttACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                                                                                                                                                <  1:622405/275‑1 (MQ=255)
                                                                                                                  tACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCTCCACGCCGGTATAGCGAACACGGATAGAGAGATAATCTCCATCCGCGCGACCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACAACCAGCGGTAAATa                                                                                                                                                       >  2:478118/1‑276 (MQ=255)
                                                                                                                    cGCCCGTGCCG‑ACCGGGAAGAAGGGATCAAACAAGCCCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                     <  2:117552/276‑1 (MQ=255)
                                                                                                                     gCCCGTGCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAGAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                     <  2:39331/275‑1 (MQ=255)
                                                                                                                          ttCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg                                                                                                                                                >  2:631132/1‑275 (MQ=255)
                                                                                                                           ttcccacctaacagaagGGATCAAACAAGCGCACACCTCCTTTCGCCGGTAGCCCCGCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg                                                                                                                                                <  3:595882/263‑1 (MQ=255)
                                                                                                                            ccG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAtctgtctctt                                                                                                                                               >  1:405682/1‑265 (MQ=255)
                                                                                                                                 ccTGGAGGAAGGGATCAAAAAACCAACCCACTCCGTTACCCGGAAGCCCCAAGCAGATATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAAAATGAAAAACTAGACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                          <  4:337966/275‑1 (MQ=255)
                                                                                                                                         aagGGATAAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCTAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcat                                                                                                                                  <  2:90183/275‑1 (MQ=255)
                                                                                                                                          agGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccccca                                                                                                                                                                 >  4:611396/1‑243 (MQ=255)
                                                                                                                                             gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCg                                                                                                                                                              <  2:611396/243‑1 (MQ=255)
                                                                                                                                               tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGTCCACTGGCACATCCGGTTCAATAAAGCACTAACACCATAACTGCTTGCGTACCACCCGCGGTAACTAGTTGGCGGTGCTGTGGTTCATCACTTCa                                                                                                                           >  4:535905/1‑276 (MQ=255)
                                                                                                                                                cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGACTTTTATGGCGTTACTGAACAGTTTGGCGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACCTCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTTCGTACCCACAGCGGTAAATAGTTTGCGGTGATGTGGTTCATCACTTccc                                                                                                                          >  4:629665/1‑274 (MQ=255)
                                                                                                                                                cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCGGCGGTAAATAGTTGGCGGTGGTGTGGTTCATCACTTcc                                                                                                                           >  3:325033/1‑274 (MQ=255)
                                                                                                                                                   aCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGGTTGCGGTGATGTGGCTCAACACTTCAcgc                                                                                                                        >  2:630400/1‑275 (MQ=255)
                                                                                                                                                                ctatttCGCCGGCATCCCCACGCCGTTAACGCGAACACGGATAGCGAGGTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcg                                                                                                          <  4:131230/273‑1 (MQ=255)
                                                                                                                                                                     tCGCCGGTATCCCCACGCCGGTATCGCGAACTCTGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                     <  2:60074/276‑1 (MQ=255)
                                                                                                                                                                     tCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTGCATCACTTCCCGCGGTGAAAACTCCcgc                                                                                                         >  2:326923/1‑272 (MQ=255)
                                                                                                                                                                      cGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTTGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                     <  2:49348/275‑1 (MQ=255)
                                                                                                                                                                       gCCGGTACCCCCAGCCCTGTACCGCGAACACGGAAAGAGAGATAATCGCCATCCGCGCGAAAAAGAAAAACGATACAACCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                     <  1:237171/274‑1 (MQ=255)
                                                                                                                                                                       gCCGGCATCCACACGCCAGTATCGCGAACAAGGTTAGAGAGATAATCGACTTCCCGGTGAACAGGAAAAACTTTACAGCCGTTATCGGTGAATTTTATGGCGGTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCg                                                                                                   <  4:612183/276‑1 (MQ=255)
                                                                                                                                                                                  cACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGCTCGATCTTCaa                                                                                           >  1:162977/1‑273 (MQ=255)
                                                                                                                                                                                      ccGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTTTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAATGCCACTTGCACATCCGGTTCACTAAAGAAGTACAAGCCTAACTGCTTGCGTACCACCCGCCGTAAATAGTTGGCGGCGCTGTGGTTACTCCCTTCACGCGGGGAAAACTCCCGCGGCCCGCTCTGCAACTGTTca                                                                                    >  4:686182/1‑275 (MQ=255)
                                                                                                                                                                                          tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                   >  4:575609/1‑193 (MQ=255)
                                                                                                                                                                                             cgcgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                                                                                                                                                <  2:575609/193‑1 (MQ=255)
                                                                                                                                                                                                          agagagATAATCCCCATCCGCGCGAACATGCAAAATTATACCGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTTCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATc                                                                <  2:538346/276‑1 (MQ=255)
                                                                                                                                                                                                               gagaaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCg                                                                                                   <  2:330567/233‑1 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAATCAGTACAAGCCTAACTGCTTGCGTACCACCTGCGGTAAAGATTTGGCGGTGCTGTGGTTCATCAGTTCCCCCGTTGCACACTCACGCGGCTCGACCTTGACCTGTTCCGATTACATCTTCGAGAAAttcagcaa                                                          >  4:472083/1‑269 (MQ=255)
                                                                                                                                                                                                                  aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGCTCATTACTTCACGCGGTTAAAACTCACGCGGTTGGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAatat                                                        >  2:560008/1‑276 (MQ=255)
                                                                                                                                                                                                                      gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGc                                                     >  1:544450/1‑275 (MQ=255)
                                                                                                                                                                                                                      gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGc                                                     >  1:293828/1‑275 (MQ=255)
                                                                                                                                                                                                                      gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAAAAACCGGTAatactgc                                                     >  3:478437/1‑271 (MQ=255)
                                                                                                                                                                                                                          tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACTCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGAt                                                 >  1:13918/1‑275 (MQ=255)
                                                                                                                                                                                                                              cgcgAAAATGCAAAACTATACAGCCGGTAGCGGTTAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAAtt                                             <  1:200714/275‑1 (MQ=255)
                                                                                                                                                                                                                                 gAACATGCAAAACTTTCCAGCCGTTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTc                                          <  3:544450/275‑1 (MQ=255)
                                                                                                                                                                                                                                     aTGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTTCTGTAAACGCATCGTGTCGCCATTGAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                     <  2:659164/276‑1 (MQ=255)
                                                                                                                                                                                                                                      tgaaaaaCTAGACGGCCGGTTTGGGTGAGTTTTATGGGGTTACTGGACAGGTTGGAGATGACAGGCGGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                     <  4:666910/272‑1 (MQ=255)
                                                                                                                                                                                                                                      tGCAAAAGTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                     <  1:325033/275‑1 (MQ=255)
                                                                                                                                                                                                                                        cAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAacaaca                                   <  1:158279/275‑1 (MQ=255)
                                                                                                                                                                                                                                               ataCCGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAACTTACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACGGGGACAGCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                             <  3:630934/274‑1 (MQ=255)
                                                                                                                                                                                                                                                taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATTTGGTTCATCACTTCACGCGGTGACAACTCCCGCAGTTCGTTCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATTACGCTGCAACTTTTAAAAAACAGGCTGGa                            >  3:652846/1‑274 (MQ=255)
                                                                                                                                                                                                                                                ataaggcGGTATCGGGTACTTGTTTGGCGTTACGGAACAGGTTGGAGAGTACCGTCTGTAAACGGATCGGTTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTGCAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGaa                           <  3:282113/269‑1 (MQ=255)
                                                                                                                                                                                                                                                  cAGCTGCTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCAACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGAGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAagag                        <  2:312940/276‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTTGATTTTATGGCGTTACTGGACAGGTTTGAGATGACCTGCTGTAAAACCATCCGGTCGCCCTTTAAGGCCCATGGCAAATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAagag                        <  3:501226/273‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCgaga                                                                    >  1:176308/1‑229 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGa                            >  1:501226/1‑269 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgtt                      >  2:74611/1‑275 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgt                       >  1:106493/1‑274 (MQ=255)
                                                                                                                                                                                                                                                       ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGCCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACCGCTTGCGTACCCCCAGCGGCAAATAGTTGGCGGTGCTGTGGTTCATCACTTCACGCGGTGAAAACTCACTCGGTACGATCTTCCACTGTTCCGATTCCATCTTCGAGAAAACGAGAATATCGCTGATAATTTTCAACAACAGTCTGGACAAgttgt                    >  3:333623/1‑275 (MQ=255)
                                                                                                                                                                                                                                                        gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGaa                                                                   >  2:70983/1‑227 (MQ=255)
                                                                                                                                                                                                                                                         tATCGGTGGATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCa                 <  2:397871/276‑1 (MQ=255)
                                                                                                                                                                                                                                                           tCGGTTAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATc                                                                <  4:70983/227‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGTGAATTTTATGTCGTTACTGAACAGGTTGGAGATCACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTg              <  2:113877/276‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGTGAATTTTATGGCGTTACTGAACAGGTTTGAGATGACCTGCTTTAAACGCATCCAGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATACATCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAGATAGTTGGCGGTGGTGTAGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTg              <  2:382107/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACCCGGTGAAAACTCACGCGGTTAGATCTTCAAATGTTCGGACTCAATCTTCGAGCAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTGTAGCATTGCCGTCAACAccc  >  4:168759/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                        tGGCGGTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGTTCGCCATTTAAGGCCACTGGAACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGc   <  3:179153/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                         
GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCG‑ACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCC  >  NC_000913/2317822‑2318360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A