Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F39 I1 R1 11 203.3 4212126 89.1% 3753004 261.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG98.8% 586.6 / NA 168L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (84/79);  minor base A (1/1);  total (86/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

GCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317818‑2318366
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                 
gccctgttaaCTCGCTTCCCACTCCCTGTACTGGATCTACCGACACATCGCCGACCATCATGCTTGGCAGCTTGTCACAAGTCGCCAGCGACAGACCCGTCCCCTTGAAAGTACGCTGTACTCCCGTTCCGACCAGGAAGAAGGGATCAAACAAGCGCAACACTGCTTTCTCCGGTATCGCCACGCCGGTATCGCGAACACGGGTAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                 <  4:354099/272‑1 (MQ=255)
gCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                 <  4:923318/280‑1 (MQ=255)
gCACAGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCCGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                 <  2:838128/280‑1 (MQ=255)
     gTAAACTTTCTGCCCATTCCCCGTTCTTAATCTACCGTTCTATCTCCGTCCATCATTCTTATCATTTTTTCACAACTCGCCAGTCCCAGACCGGTCCCCTGGAAATTTCGCTTTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                 <  1:856057/275‑1 (MQ=255)
     gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                 <  1:304525/275‑1 (MQ=255)
            ggagtcccAGTACCGATTTTGAATTTACCGAAATATCACCGTCCATGATGCTGTACAGGATTGCCCAAATGTCCATACCCAGACCGGTCCCCTGGTAATTACGCTGTTCGCCCGTTCCGACCTGGCAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGTGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg                                                                                                                                                                                                                                                                             <  3:780361/267‑1 (MQ=255)
              cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGAACAGTTTTTCACAAATCGCCAGACCCAGACCGCTCCCCTGGAAATTACGCTGTACGCTCGTTCCGACCTGGAAGAAGGGCTCAAACAAGCGCACCACTTCTTTCGCCGGTATCACTACGCCGGTATCGCGACCACGGACAGAGAGATAATCGCCTTCCGCGGAAACATGCAAACAGATACAACCTGGCTCCGCGACTTTTATGGCTTTACTCacagggatgga                                                                                                                                                                                                                                                                        >  3:882958/1‑265 (MQ=255)
               tGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAACGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTACCGGTGAATTTTCTGGCCTTACTGACAAGGTTGgag                                                                                                                                                                                                                                                                       >  1:532062/1‑275 (MQ=255)
                gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATAATCGCCATCCGCGCGAACTTGCCAAACTATAAAGCCGGTATCTGTGACTTTTTTGGCGTTACTGAACCGGTTGGGAATGAc                                                                                                                                                                                                                                                                  >  2:901991/1‑279 (MQ=255)
                gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGGGAATTTTATGGAGTTACTGAACAGGTTGGAGATGAcc                                                                                                                                                                                                                                                                 >  2:511722/1‑280 (MQ=255)
                gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTACGCCCTGGAAGAAGGGATCAAACAAGCGCACCACTTCATTCGCCGGTATCCACACGCCGGTATCGCGAACACGGATAGAGAGATAACGCCCATCCCAGCGAACATGCAAAAATCTACAACCGGAAGCGGTAAATTTTCTGGCGTACCTGACAAGGTTGGAG‑TGACct                                                                                                                                                                                                                                                                >  4:344776/1‑280 (MQ=255)
                gCCCAGTCCCGGTTCTGAATCTACCGAGATATCGCCGGCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAcc                                                                                                                                                                                                                                                                 <  2:400414/280‑1 (MQ=255)
                    aTTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCGGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                                  <  3:846070/275‑1 (MQ=255)
                            ttCTGAAGCTACCGAGATAGCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAAACCGGGCCCATGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCAGCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAAGTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGt                                                                                                                                                                                                                                                           <  1:348342/274‑1 (MQ=255)
                                     caCCCAGACATCGCCGTCCCTCATGCTGACCAGTTATTCCCAAACCGCCCGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGNGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTc                                                                                                                                                                                                                                            <  4:579690/278‑1 (MQ=255)
                                           gcgatCGCCGTCCCTCATGCTGATCAGTGTTTCACAAACCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCg                                                                                                                                                                                                                                           <  1:941969/271‑1 (MQ=255)
                                           gaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGCCCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCg                                                                                                                                                                                                                                           <  1:604183/274‑1 (MQ=255)
                                                 gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCACGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCCCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGGACAGGTTGTAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTCAGGc                                                                                                                                                                                                                                >  2:880109/1‑279 (MQ=255)
                                                   cGTCCATCATACTGATCAGTTTTTCCCCAATCGCCAGACCCAGACCGGGCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAg                                                                                                                                                                                                                                  <  1:226547/275‑1 (MQ=255)
                                                     tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTCCTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCACCGGGTCGCCATTTATGGCCAccg                                                                                                                                                                                                                           >  2:458774/1‑278 (MQ=255)
                                                       aaccatGCTGATCAGTCTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAGTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGc                                                                                                                                                                                                                         <  2:531239/277‑1 (MQ=255)
                                                            tggtgatccactttcCACAACACGCAAGAACCGAAACGGGCCCCTGGAAACTACGCTGTACGCCCGTTCCGACCGGGAAGAAGGGATCAAACAAGCGCACCACGTCTAACGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAGCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGGTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                    <  4:458543/269‑1 (MQ=255)
                                                            tGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGc                                                                                                                                                                                                                         <  1:737653/275‑1 (MQ=255)
                                                            tGCTCATCACTTTTTCCCAAATGCCCAGACCCAAACCGGTCCCCTGGAAATTCCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                    <  4:511722/280‑1 (MQ=255)
                                                                 aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGtt                                                                                                                                                                                                               >  2:760532/1‑280 (MQ=255)
                                                                 aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCCGGAAATTACGCTGTACGACCGATACGACATGGAAGAAGGGATCAAACAAACGCACCACTTACTTCGCCGGTATCCCCACGCAGGGATCGCGAACACGGACAGAGAGATAATCGCTCGCCGCGCGAACATGCAAAACTATATAGCCGGAATCGGTGAATATACTGGCGTTAGTGAACACGATGGAGATGACCGGCTGTAACACGAAT‑GGGTCGCCATTTAAGGCCACTGGCACGTc                                                                                                                                                                                                                    >  3:606270/1‑275 (MQ=255)
                                                                    aGTTTTCCACAACACCCCAGACCCAGGCCGGGCCCCCTGAAAATAGCCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCCCTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                            <  4:409845/280‑1 (MQ=255)
                                                                     gTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                            >  4:1007828/1‑279 (MQ=255)
                                                                      tttttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGtt                                                                                                                                                                                                               >  1:796172/1‑275 (MQ=255)
                                                                        tttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTTGAGATGAACTGCTGTAA‑ACTCACCGGGGAGCCATTTAAGGCCACTGGCACACCCGGttaa                                                                                                                                                                                                             >  3:206192/1‑273 (MQ=255)
                                                                         ttCACAAATCGCCAGACCCATACCGGGCCCCTGGAAATTACGCTGTACTCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  2:952434/280‑1 (MQ=255)
                                                                         ctacaaaaCCGCCAAACACAGACCGAGCCACTGGAAATTACGCTGGACGCCCTTTCCGACCAGGAAGAAGGGATCAAACAAGCGAACCACTTCTTGCGCCGGTATCCCCAAGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  4:818866/275‑1 (MQ=255)
                                                                            acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACAGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATaa                                                                                                                                                                                                         <  3:385254/275‑1 (MQ=255)
                                                                              aaaCCGCCAGACCCCGACCGGTCCCCTGGAAATTACGCTGTACCCCCCTTCCGACCTCGAAGAAGGGAGCAAACAAGCGCACCACGTCTATCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  3:750001/275‑1 (MQ=255)
                                                                                    ccagcaccagaccTGTCCCCTGGAAAGTACGCTGTACGACCGTTCCGACCTGGAAGAAGTGATCAAACAGGCGGGCCACTTCTGTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGTAAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGGATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                                 <  3:148284/269‑1 (MQ=255)
                                                                                          ccacaacGGTCCCCTGGCAAGTCCGCTGGACCCCAGTTCCGAACTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGTACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAAc                                                                                                                                                                                        <  4:63196/272‑1 (MQ=255)
                                                                                                cGGTCCCCTGGAAATTAAGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGc                                                                                                                                                                                     <  1:727559/275‑1 (MQ=255)
                                                                                                   tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACGACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGCACACGGATAGCGAGATAATCCCCATCCGCGCGAACATGCACAACTATCCAGCGGGTATCGGTGCATTTTACGGCGTTACTGAACAGGTTGGCGATGACCTGCTGTAA‑CCGCCTCTGGTAGCCACTTACGCCCACTGGCACATCCGGTTCAAAAAACCAGTACAAGCCTAACTGGTTg                                                                                                                                                                                  >  3:194885/1‑275 (MQ=255)
                                                                                                   tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTg                                                                                                                                                                                  >  1:828348/1‑275 (MQ=255)
                                                                                                   tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGACTAACTGCTTGCGTac                                                                                                                                                                             >  2:502640/1‑280 (MQ=255)
                                                                                                      ccTGGAAATGACGCGGTACGCCCGTTCCGACCTGGAAGAAGGAATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                          <  2:217094/280‑1 (MQ=255)
                                                                                                          gAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGACTTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATACGGTTCACTAAAGCCGTACACACCTATCTGCTTGCGTacca                                                                                                                                                                           >  3:925860/1‑275 (MQ=255)
                                                                                                          gAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGACCAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCCCCACCGGCTCAATCAAGCAGTACAAGCCATACTGCTTTCGTacaa                                                                                                                                                                           >  3:434325/1‑273 (MQ=255)
                                                                                                             aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                          <  1:246549/273‑1 (MQ=255)
                                                                                                             aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGCATCGCGACCACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATGTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTCTAA‑CCGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCATAAAATCACTACAAGCCTAACTCCTTTCGTaccacc                                                                                                                                                                         >  3:564501/1‑274 (MQ=255)
                                                                                                                 agCTGTTCGCCCGCTGCGACCTTAAAGAAGGGAACAAACAAGCGCCGCACTTCTTTCGCCGGGAGCACCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCAACCGCGCGAACATGCAAAACTATACAGGCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGACATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAg                                                                                                                                                                       <  3:532062/271‑1 (MQ=255)
                                                                                                                            cGTTCCGACCTGGAAGAAGGGATCAAACAAGCAAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTgg                                                                                                                                                        <  1:794362/275‑1 (MQ=255)
                                                                                                                              ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCCTTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCgggta                                                                                                                                                  >  2:998011/1‑276 (MQ=255)
                                                                                                                               tCCGACCTGGAAGAAGGGCTCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCgg                                                                                                                                                     <  1:398240/275‑1 (MQ=255)
                                                                                                                                ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCTTTACTGAACAGGTTGGAGATGACTTGCTGGAA‑ACGCATCGGGTCg                                                                                                                                                                                                                                           >  4:113336/1‑189 (MQ=255)
                                                                                                                                     cTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGAtg                                                                                                                                                <  1:643560/274‑1 (MQ=255)
                                                                                                                                              gggACCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTGATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGTTGTgggttaacc                                                                                                                                      >  1:949091/1‑268 (MQ=255)
                                                                                                                                                  tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAt                                                                                                                                                                                                           >  3:727918/1‑203 (MQ=255)
                                                                                                                                                  tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGc                                                                                                                                                                      >  2:11826/1‑240 (MQ=255)
                                                                                                                                                  tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcgc                                                                                                                              >  2:631674/1‑279 (MQ=255)
                                                                                                                                                  tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAAGCCACTGGCACAGCCGGTTCAATAAAGAAGTACAACCCTAACTGCTGCCGTACCACCAGC‑GGTACACCTTTGGCGTTGATGTGGTTACTCACTTCAcccg                                                                                                                             >  4:796093/1‑280 (MQ=255)
                                                                                                                                                          gcgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTTGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCACC‑GGTAAATAGTTGGCGGTGATGTGGTTCATTACTTCACGCGGTg                                                                                                                          >  1:724970/1‑275 (MQ=255)
                                                                                                                                                           cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAATATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATACTTTGCGGTTCTTTGGTCCATCCCTTCATTCGTTGAAAACt                                                                                                                    >  2:241505/1‑280 (MQ=255)
                                                                                                                                                                  cttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                            <  4:735577/186‑1 (MQ=255)
                                                                                                                                                                  cttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                            >  2:735577/1‑186 (MQ=255)
                                                                                                                                                                     cttTCGCCGGTTGCACCACGCCGGTATCGCCAACACGGGTAGAGAGATAATCGCCATACGCGCGAAGATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTAATGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTAGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:666440/279‑1 (MQ=255)
                                                                                                                                                                         cGCCGGTATCCCCCCGCCGGTATCGCGAACACGGATAGAGAGATTATCGCCATCCGCGCGAACATGCAACACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  1:882958/275‑1 (MQ=255)
                                                                                                                                                                          gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCa                                                                                                                                                                                                     >  3:333064/1‑185 (MQ=255)
                                                                                                                                                                          gCCCGTCTCCCCACACCGGTATCGCGAACACTGATAGATAGATAATCGCCATCCACGCGAACATGCAAAACTGTACAGCCGGTATCGGTTAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCTGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCGAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  3:773959/274‑1 (MQ=255)
                                                                                                                                                                            cGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAACTGCTGTAA‑ACGTATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCTGTGATGTGGTTCATCACTTCACGCCGTGACACCTCACGCGGTTCGATCTTc                                                                                                   >  2:376190/1‑280 (MQ=255)
                                                                                                                                                                               tATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTg                                                                                                                                                                                      >  3:279073/1‑195 (MQ=255)
                                                                                                                                                                                   ccccggCCGGTACCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAAAATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCaa                                                                                                 <  3:151681/270‑1 (MQ=255)
                                                                                                                                                                                   cccACGCCGGTATCGGAAAGACGGAGAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATTGCGTTACTGAACAGGTTGGAGATGACCGGCTGTAA‑ACGCATCTGGTCGCTATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGtt                                                                                            <  2:344776/280‑1 (MQ=255)
                                                                                                                                                                                        gCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGAGTACCCCCCGC‑GGTAAATAGTTGCCGGTGATGGGGATCATCACTTCACGCGGTGAAAACCCACTCGGTTCGATCTTCCACTGTTCCg                                                                                         >  4:174281/1‑278 (MQ=255)
                                                                                                                                                                                         ccGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTc                                                                                           >  1:995866/1‑275 (MQ=255)
                                                                                                                                                                                                                 agaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATGGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATCACCTGCTGTAA‑ACGCATCGGCTCACCATTTAACGTCACTGGCACACCCGGTTCAATAAGGCAGTACACGCCTAACTGCTTGCGTATCACCATC‑GGTAAATAGTCGGCGGTCATTTGGTTCACCACTTCCCCCGGTGAACACTCAAGCGGATCGATCTTGACCTGTCGCGATTCCATCTTCAAAAATTCgta                                                                   >  3:39137/1‑273 (MQ=255)
                                                                                                                                                                                                                 agaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCgag                                                                   >  1:167002/1‑275 (MQ=255)
                                                                                                                                                                                                                 agaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCCAGAatat                                                              >  2:12770/1‑280 (MQ=255)
                                                                                                                                                                                                                   aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                 >  1:1017348/1‑275 (MQ=255)
                                                                                                                                                                                                                   aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCg                                                            >  2:1039654/1‑280 (MQ=255)
                                                                                                                                                                                                                     aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCATCTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCga                                                           >  2:633212/1‑279 (MQ=255)
                                                                                                                                                                                                                     aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCt                                                          >  2:424976/1‑280 (MQ=255)
                                                                                                                                                                                                                         gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGc                                                           >  1:42957/1‑275 (MQ=255)
                                                                                                                                                                                                                             tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGtt                                                                                            <  1:668837/238‑1 (MQ=255)
                                                                                                                                                                                                                             tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCAATTCACGCGGTGAAAACTCACGCGGTTCGATCTTCACATGTTCCGATTAAATCTTCGATAAATCGAGAATATCGCTGATACttt                                                  >  2:953959/1‑280 (MQ=255)
                                                                                                                                                                                                                             tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTGCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGtt                                                                                            >  3:668837/1‑238 (MQ=255)
                                                                                                                                                                                                                             tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTCAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGTTCCATTCTTCGAGAAATCGAGCATATCGCTGATAAttt                                                  >  2:935799/1‑280 (MQ=255)
                                                                                                                                                                                                                              ccgaccaaaaaTGCAAAACTATAACGCCGGTATCGGTGCATTTTATGGCGATACTGAACAGATTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGa                                                        <  1:123733/263‑1 (MQ=255)
                                                                                                                                                                                                                                gcgagAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATaa                                                     <  1:62682/274‑1 (MQ=255)
                                                                                                                                                                                                                                    gAATATGCTAAACTCTACAGCCGTTATGGTTGAATTTTATCGCGTTACTGAACCGGTTGGAGATGACCTGCTGTAA‑ACGCATAGCGTCGCCATTTAAGGCCACTGGCACATCCTGTTCAATAATGCAGTACAAGCCTAACTGCTTTCGTACCACCAGC‑GGTGAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                           <  2:198149/280‑1 (MQ=255)
                                                                                                                                                                                                                                            agactgataCAGCCAGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCGGCTGTAA‑ACGCATGGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                   <  2:261026/274‑1 (MQ=255)
                                                                                                                                                                                                                                               accataCAGCCTGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCTGTTCAATAACGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGaa                                 <  2:250075/276‑1 (MQ=255)
                                                                                                                                                                                                                                                   taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGAATGGAGATAACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGACACATCCGGTTCAATAAAGCAGTACAAGCATAACTGCTTGCGTAACACCAGA‑GGTAAATAGTTTGCGGTGATGTGGTTCATCAATTCACGAGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTAAACAACAGGCAGGCAAAgt                             >  2:57526/1‑279 (MQ=255)
                                                                                                                                                                                                                                                        ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTACGATTCAATCTTCGAGAAATCGAGCATATCGCTGATAATTTTCAACAACAGGTTGGAAGAgt                             >  1:906412/1‑274 (MQ=255)
                                                                                                                                                                                                                                                          ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCAtt                     >  2:257075/1‑280 (MQ=255)
                                                                                                                                                                                                                                                           ggatgggTGAGTTGTATGGCGTTCCTGAACAGGTGGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTg                    <  2:16797/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACCAGCATAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTAATTCTTCGAGAACTCTAGAATCTCCCTGCAAATTTTCAACAACAGGCTGGAAGCGGTTTTCCTTgtc                  >  4:261026/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                     atgttAGGGCGTTACTTAACGGTTTGGAGAGGACCGCCGTTAA‑ACGCATCTGGTCGCCAATTAAGGCCCCTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCNTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCa           <  4:540881/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                      ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGTCGAAAACTCACGCGGTTCGATCTGCAACTTTTCCGATTCAACCTTCGAGAAATCGAGAATATCGCTACAAAGTTTCACACACACGCTGTAAGAGTTTTTCATTGCAGTTCCCAg          >  4:597372/1‑279 (MQ=255)
                                                                                                                                                                                                                                                                      ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCga                                                                    >  3:590101/1‑221 (MQ=255)
                                                                                                                                                                                                                                                                          aTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAat                                                                <  1:590101/221‑1 (MQ=255)
                                                                                                                                                                                                                                                                              cGTTACTAAACAGGTTGGAGATGACCTGTTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGa  <  2:287172/279‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ttACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAGGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACtt                                                                                                                                   >  1:190480/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                                ttACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATc    >  1:584144/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                ttACTGAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATc    >  1:295530/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                 
GCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAA‑ACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGC‑GGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317818‑2318366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A