Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F34 I1 R1 9 132.5 2962696 84.5% 2503478 259.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG96.4% 374.2 / NA 115L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (50/56);  minor base A (4/0);  total (57/58)
Fisher's exact test for biased strand distribution p-value = 5.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 115 total aligned reads displayed.

CTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317828‑2318366
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                  
ccgtcTGCCCATTCCCCGTTCTGAATCTACCGAGAGAGCGCCGTCCATCATGCTGATCATTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCGCCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  4:459890/271‑1 (MQ=255)
cTGGCAGCCGCTTCCCGGTTCTGAAACAACCGATGTATCGCCGTCCATCATGCTGATCAGTTGTGCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCGGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  2:335028/275‑1 (MQ=255)
    cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGGGAATTTTATTGCGTTACTGAAAAGGTTGga                                                                                                                                                                                                                                                                         >  2:59584/1‑275 (MQ=255)
     ctccccTTCCCGGTCCCGAACCACCCGAGAATACCCCGTCCACCATGCTGATCAGTTATTCCCACACCGCCACAACCAGACCGGTCCCCCGGAAATGACGCTCTACTCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCTCCGGTATCCCCACGCCGGGATCGAGAACACGGATAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgag                                                                                                                                                                                                                                                                        <  4:170470/273‑1 (MQ=255)
      gcgcATGCCCGTTTCTGAATCTACCGGGATATCGCCGTCCATCATCCTGATCAGTTTTTCACAAATCGCCGGACCCAGACCGGTCCCCTGGTAATTACGCTGTACGCCCGTTCCGACCTGAAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTGTATGGCGTTACTGAACAGGTTGgag                                                                                                                                                                                                                                                                        <  1:554432/271‑1 (MQ=255)
      gCCCATTCCCGGTTCTGAATCTACCGAGATATCTCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGCCCGGTCCCCTGGAAATTACGCTGTACGCCAGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCCAACACGGATCGAGAGATAACCGCCATCCGCCCGAACATGCAAAACTAGCAAGCCGGTATCGGTGAAATTTATGGCCTTACGAAACAGGTTGgaga                                                                                                                                                                                                                                                                       >  3:629750/1‑275 (MQ=255)
      gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGCAGGGCTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGTTAATCTCCATCCGCGCCAACCTGCCAAACCACACAGCCGGTAACGGTGAATTGTATGGCGACATTCAACAGTTTGcagc                                                                                                                                                                                                                                                                       >  3:317118/1‑274 (MQ=255)
          aTTCCCGGTCCTGAATCTACCGAGATATCGCCGTCCATCATGCGGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  1:317118/260‑1 (MQ=255)
                 gTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTAACCCTACGCCGGTATAGCAAACACGGAATGAAAGATAAACGCCACCCGCCCGAACATGACAAACTATACAACCGGGATCAGTGACTTTTCTGGCGTTATGGAAAAGGTATGAGATGC‑Ccttgtgt                                                                                                                                                                                                                                                           >  3:382107/1‑270 (MQ=255)
                            aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTTTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCAGGTATCGCGAACACGCATAGAGAGATAATCGCCATCCCCCCGCACATGCAAAACTACACCGCAGAGATCGGTGAAGTTTTTGGCGTTACTGAACAGGTTGAAGATGA‑CGT‑GCGGTAAACGCATctg                                                                                                                                                                                                                                               >  3:161646/1‑273 (MQ=255)
                                 gagtgcGCCGTCCTACATGCTGATACGGTTTTCACCCAGCGCCACACCCCGACCGGTCCCCTTGAAATTACGCTGTACGCCCGTTCCGAACTGGACGAAGGGATCAAACACGCGCACCACTTCTTGCGCCGGTATCCCCAAGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCg                                                                                                                                                                                                                                           <  4:68972/269‑1 (MQ=255)
                                 gaTACCGCCGTCCATCATGCTGATCAGTTTTTCACAAAACGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGc                                                                                                                                                                                                                                          <  1:436619/275‑1 (MQ=255)
                                  atatCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGATCCAGACCGGTCCCCTGGAAATTACGCTGTACGCTCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACTACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGACCACGGATAGAGAGATAATCGCCATCCGCGCGCACCTGCAAAACTATGCAGCAGGTAACGGTGAATTTTATGTGGTGACGAGACAGGGTGGAGCTTA‑CCT‑GCTTGAAACGGATCGGGtaccc                                                                                                                                                                                                                                         >  4:248188/1‑271 (MQ=255)
                                       gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATCGAGAGATAAGCGCCATCCGCGCGAACCTGCAAAACTATACAGCCGGTACCGATGAATTTTTTGGCGTTAATGAACCAGTTGGAGTTGA‑ACG‑TCGATAAAAGCATGTGGTCGCAAtgt                                                                                                                                                                                                                                     >  2:605394/1‑272 (MQ=255)
                                         tgTCCATCATGGTGAGCAGTGTTTCCCCACTCGCCAAACGCAGACCGGGCCCCTGGAAATTAGTGTGTACGCCCGTTCCGACCTGGAAGAAGAGATCAAACAAGCGCAGCACTTCTTTCGCCGGTATCCCCACGCCGGTAGCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGGGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                                                                                                                                                                  <  4:407467/274‑1 (MQ=255)
                                           tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTTGAGACGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGc                                                                                                                                                                                                                                >  3:489021/1‑275 (MQ=255)
                                             catcaaGCTGATCAGTTTTTCCCAAACCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                              <  1:221975/275‑1 (MQ=255)
                                             catcaaGCGTATCAGTTTTTCCCAAATCGCCAGACCCAGGCCGGGCCCCTGGAAATTACGCTGTACGCCCGGTCCGACCAGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                              <  1:449568/275‑1 (MQ=255)
                                                  tGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGACCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc                                                                                                                                                                                                                         <  1:765443/275‑1 (MQ=255)
                                                  agCTGATCAGTTGTTCACAAATAGCCAGCCCCAGACCGGTCCCCTAGAAAGTACGCTTCACGCCCGTGCCGACCTGGAAGAAGGAGTCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGTACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc                                                                                                                                                                                                                         <  3:30421/274‑1 (MQ=255)
                                                     ttatacgtgtttCCCAAAGCGCCAGACCCAGACCGGCCACCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGcaca                                                                                                                                                                                                                      <  2:17370/266‑1 (MQ=255)
                                                       accAGTTTGTCAACAATCGCCAGACCCCGACCGGTCCCCTTGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCGTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                    <  2:643129/273‑1 (MQ=255)
                                                       aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCCAACATGCAAAACTATCCAGCCGGTATGGGTGACTCTGATGGCTTTACTGAACAGGTTGGACAGGA‑CCC‑TCTGTAAACACATGGGTTCGCCATTTAAGTCCatttgcgca                                                                                                                                                                                                                      >  3:153651/1‑265 (MQ=255)
                                                          cggttttTACAAATCGCCAGCACCAAACCGGGCCCGTGGAAGTTACGCTGGCCGCCCGTTCCGGACTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATATAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  2:352567/272‑1 (MQ=255)
                                                          agtactccaccAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  2:85959/268‑1 (MQ=255)
                                                          aggttttCACAAATCGCCAGCCCCAGACCGGGCCCCTGGAAAGTACGCTGTACCCCCGTGCCTACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  2:620737/272‑1 (MQ=255)
                                                          actttttCACACATCGCCAGACCCAGACCGGTCCCCTGCAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACGTCCTTCGCCCGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATAATCGCCATCCCCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  1:156864/273‑1 (MQ=255)
                                                          aGTTCTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAACTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  2:259839/275‑1 (MQ=255)
                                                          aGTTCTTCACAAACCGCCAGCCCCCGACCAGCACGCCAGAAATGACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACTGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGNATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                 <  4:337928/275‑1 (MQ=255)
                                                           gTTTTTCACAAATCGCCAAACACAGACCTGTCCCCTGGAAATTACGCTGTACACCCGTTCCGACCTCGAAGAAGGGATCAAACAAACACACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGAAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGGGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACGGGCACATCCgg                                                                                                                                                                                                                 <  1:393259/274‑1 (MQ=255)
                                                           gTTTCTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACAt                                                                                                                                                                                                                     <  1:153651/270‑1 (MQ=255)
                                                            tttttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGCTAGAGAGATAATCGCCATCCGCGCGAACCTGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCG‑GCTGTAAACGCATCGGTTCGCCATTTAAGGCCCTCGGCAAATCCGGtt                                                                                                                                                                                                               >  1:222613/1‑275 (MQ=255)
                                                                    aaaTCGCCAGACCCAGCCCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  1:559773/275‑1 (MQ=255)
                                                                    aaaTCCCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  1:29114/275‑1 (MQ=255)
                                                                    aaaGCGCCAGACCCCGACCGGTACCCTGGAAATTACGCCTTACGCCCGTTACGAGCTGGAAGAAGGGATCAAACAAGCCCACTACGTCTTTCGCCGGATTCCCCACGCCGGTATCGCGAAAACGGATAGATAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGGAGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  2:352538/275‑1 (MQ=255)
                                                                              acccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCAc                                                                                                                                                                                                                             >  1:544358/1‑243 (MQ=255)
                                                                                cccgaccGGTCCCCAGGAACTTACGCCTGACGCCCGTGCCGACCTGGAAGAAGGGATCAAACAAACGCACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCt                                                                                                                                                                                          <  4:524539/272‑1 (MQ=255)
                                                                                  agaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGc                                                                                                                                                                                                                         <  3:544358/243‑1 (MQ=255)
                                                                                   gaccGATCCCCTGAAAATTACGCGGTACGCCCGTTCCGACCTGGAAGAAGGCACCAAACAAACGCACCACTTCTTTCGCCGGTATCCCCACGCCTGTATCGCGAACACGGTAAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAAc                                                                                                                                                                                       <  2:337211/275‑1 (MQ=255)
                                                                                        gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTTAACAGGTTGGAGTTGA‑CCT‑GCTGTAAACGCATGGGGTCACCATTTAAGGCCACTTGCCCATCCGGTTCACTAAAGC‑AGTACAAGCTCAACTGcat                                                                                                                                                                                  >  3:46012/1‑273 (MQ=255)
                                                                                        gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCAGGTTCAAt                                                                                                                                                                                                           >  1:170579/1‑251 (MQ=255)
                                                                                         tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCCAACACGGATAGAGATATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTACTGATGAATTTTATGGCGTTACTAAACCTGTTGGACATGA‑CTT‑GCTGTAAATGCATGTGGTCGCCATTTAAGGACACTCCCAACTCCGGCT‑TATATAGCAAGTACAAGCGTACCTGcctc                                                                                                                                                                                 >  3:82519/1‑272 (MQ=255)
                                                                                         tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGNATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACAATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCAATGGCACCTACGGTTCAATAAAAC‑AGTAAAAGACTAAGTGCttt                                                                                                                                                                                 >  4:341396/1‑274 (MQ=255)
                                                                                            ccTGGAAATTACGCTTGACTCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACGTCTGTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                       <  3:170579/251‑1 (MQ=255)
                                                                                                 aaaTTTCGCTGTACGCCCGTTCCGACCTGGAAAACGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                         <  2:671189/275‑1 (MQ=255)
                                                                                                   aTTACGCTGTACGACCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                       <  2:97129/275‑1 (MQ=255)
                                                                                                              cGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGCATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTAAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCCGCGGTAAAtga                                                                                                                                                            >  1:6275/1‑273 (MQ=255)
                                                                                                                    ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CAT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCAACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAACGGTAAATAGTTgcc                                                                                                                                                       >  2:513090/1‑272 (MQ=255)
                                                                                                                      ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAGAGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GATGTAAACGCATCGGATCGCCCTTTAAGGCTCCTGGCAAATCCGGTTCACAAAAGC‑AGTAAAAGCCCAACTGCATGCGTACCACCAGCGGAAAATAGTTGGCgg                                                                                                                                                     >  2:323020/1‑274 (MQ=255)
                                                                                                                          ccTGGAAGAAGGGATCAAACACGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  2:462797/148‑1 (MQ=255)
                                                                                                                           cTGGAAGAAGGGATCAAACAAGCGCAGCACTTCTTTCGCCGCTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCACGCGAACATGCCAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTAGAGATGA‑CCT‑GCTGTAAACGCATCGGATCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                                <  1:213247/274‑1 (MQ=255)
                                                                                                                             ggAAGAAGGAATCAAACAAGCGCACCACTTACTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTgg                                                                                                                                             <  2:277152/275‑1 (MQ=255)
                                                                                                                                      gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTCTACAGCCGGTATCGGTGAATTTTATGGCGGTACTGAACCGGTTGGAGATGA‑CCT‑GCTGTAAACGAATCGGGTCGCCATTTAAGTCCACTGGCACATCCGGTTAAATAAAGC‑AGTACAAGCCTAACTGCTTTGGGACCACCCGCGGTAAATAGGTGGCGGTAAGCTGGTCCATCCCt                                                                                                                                    >  2:643558/1‑275 (MQ=255)
                                                                                                                                           agcAAGCCCACCACTTCTTTCGCCGGTTTACCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGAGAACAGGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCAtcacgtcacg                                                                                                                               <  1:382107/273‑1 (MQ=255)
                                                                                                                                               aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCAATGGCACATCCGGGTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCACCGGTAAATAGTTGGCGGTTATGTGGGTCTTCATTTccccgggt                                                                                                                           >  3:607748/1‑268 (MQ=255)
                                                                                                                                                gcgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTg                                                                                                                          >  2:11663/1‑275 (MQ=255)
                                                                                                                                                          tcttTCGCCGGTAGCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATGCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCAGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcg                                                                                                                <  2:248188/275‑1 (MQ=255)
                                                                                                                                                               cGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAACGCCATCCGCGCAAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  1:563672/275‑1 (MQ=255)
                                                                                                                                                               cGCCGGTACCCCCACGCCGGTATCGCGAACACGAATAGCGGGATAATCGCCATCCGCGCGAACATGCAAAGCTATACCGCCGGTATCGGTGAATTTTATGGCGTTACGTAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:178907/275‑1 (MQ=255)
                                                                                                                                                                gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:365535/274‑1 (MQ=255)
                                                                                                                                                                    ggaTCCCACCGACGGTACCACTAAAACGGAATGAAGGATAATGGCCATCAGGGCGAAAATGAAAAAATATGCCGCCGGTATCGGGTAAGTTTATGGCGTTACTGAAAAGGTTGGGGAGAA‑CCT‑GCTGGAAAAGCAGCGGTTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTGGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                      <  4:643558/273‑1 (MQ=255)
                                                                                                                                                                    gaaaccccACGCCGGTATCGCGAAACTGGATAGAGAGTAAATCGCAATCCCCGCGAAATTGAAAAACTATACAGCCGGTATCGGTTAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                      <  2:640998/271‑1 (MQ=255)
                                                                                                                                                                    gaaTCCCCAGGCCGGTATCGCGAAGACGGATAGAGAGATAATGGCCATCCGACCGACAATGCAAAACTATACAACCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTATAAACGCATCGAGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                      <  3:607815/273‑1 (MQ=255)
                                                                                                                                                                                        cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATAGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGAGTACCACCAGCGGGAAATAGTTGGCGGTGATGTGGATCCTCACTTCACGCGGAGACAACACACCAGGATCGACTTTCCACTGTTCCGGttacca                                                                                  >  4:60450/1‑271 (MQ=255)
                                                                                                                                                                                                 aTAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGTCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACAACAAGCGGTACATAGTTGGCGGTGATGTCGTTCATCACTTCAACCGGTGAACACTAATGCGGTTCGCTCTTGAACTGTTCCTGTTCAATCtcacaga                                                                          >  4:109499/1‑268 (MQ=255)
                                                                                                                                                                                                       agaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGAGGTAAATAGTTGGCAGTGCTGTGGTTCATCACTTCCCGCGGTGAAACCTCACGCGATTCGATATTCAAGTGTTCCGCTTCATTCTGCCAGAAAACgat                                                                   >  2:197461/1‑274 (MQ=255)
                                                                                                                                                                                                         aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCCGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGGTCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGTGaa                                                                 >  1:331653/1‑275 (MQ=255)
                                                                                                                                                                                                           aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGCATTTTATGGAGTTAATAAACAGGTTGGAGATGA‑CTC‑GCTGTAATCGCATCGGCTCGCCATTTAAGGCCACTGTCACCTCCGGTTCACTAAAGC‑AGTATAAGCCTAACTGCTTAAGTACCACCCGCGGTAAATCGTTGGCGGTGATGTGGACCATCACTTCACGCGGTGAAAACTCACGCGGTTCGTTCTTCACGTGTTCCGATTCCATCTTAGATAAATCgagactc                                                               >  3:17651/1‑272 (MQ=255)
                                                                                                                                                                                                           aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAat                                                                >  1:63153/1‑274 (MQ=255)
                                                                                                                                                                                                           aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTAACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAata                                                               >  3:686848/1‑275 (MQ=255)
                                                                                                                                                                                                           aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGATGGCGTTGATGTGGGTCATCACTTCACGCGGTGAAAACTAACGCGGGTCGATCTTCGACTGTTCCGATTCGTTCTTCGAGTAATCGAGaaa                                                                >  2:511621/1‑273 (MQ=255)
                                                                                                                                                                                                                      ccgcgAAAAGGAAAAACTATACAACCTGTATCGGTTAATTTTATGGCGTTACTGAACAGGTTGGAGATTA‑CCG‑GCGGTAAACGCATCGAGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATaa                                                     <  3:331653/273‑1 (MQ=255)
                                                                                                                                                                                                                       cgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGAGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCTTCACTTCACGCGGTGAAAACTCACGCGGTTAGATCTTCAACTGTTCCGATTCAATCTTCGCAAAATCGAGAataacccctgtctc                                                    >  3:431304/1‑263 (MQ=255)
                                                                                                                                                                                                                           aaCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTTTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTca                                               >  1:473655/1‑275 (MQ=255)
                                                                                                                                                                                                                           aaCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATc                                                                      <  1:431304/252‑1 (MQ=255)
                                                                                                                                                                                                                               tgaaaaaTTATGCAGCGGGTTTCGGTGAATTTTCTGGCGTTACTGAACAGGTTGGA‑ACGAGCCC‑TCTGTAAACGCATCGGGTCGCCAGTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                           <  4:11663/272‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGCAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACACGCCTAACTGCTTGCGTACCACCAGCGGTCACTAGTTGGCGGTGATGTGATACATCAATTCACGCGCTGAAAAATACATCGGTTGGAGCTTCCTGTGTTCCGTTTCACTCTTCGAGAAATCGATAATGACGCTGAACATTTTTAACACCAGGc                                      >  3:343583/1‑275 (MQ=255)
                                                                                                                                                                                                                                      cTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATCCAATCTTCGAGAAATCGAGATTATAGCTGATAATTTTCAACATCAGCCTg                                    >  2:423442/1‑275 (MQ=255)
                                                                                                                                                                                                                                        tgtcAGCCGGTATTGTTGAATTTTATGGCGTTACTGGAAAGGTTGGAGAGGA‑CCA‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                   <  3:222613/271‑1 (MQ=255)
                                                                                                                                                                                                                                        ataCGGCCGGTGTCGGGTAATTTGATGGGGTTACTGAACAGGTCGGGGAGGA‑CCTGGCTTAAAACAC‑TCGGGTCTCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                   <  4:605394/274‑1 (MQ=255)
                                                                                                                                                                                                                                        ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGACTATCTCTTCTAATTTTCAACAACAGGCTgg                                   >  1:99568/1‑274 (MQ=255)
                                                                                                                                                                                                                                        ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCCTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGGTGACAACTCACGCGGTTCGATCTTCAACTTTTCGTATTCAAGCTTAGAGTAATCGAGATTATCCCTGATAATTTTCAACAACCGGATgg                                   >  4:287235/1‑274 (MQ=255)
                                                                                                                                                                                                                                        ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCACCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCTGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                   >  2:484463/1‑274 (MQ=255)
                                                                                                                                                                                                                                              ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCCCGCGGTGAAAACTCAAGCGGTTCGATCTTCCACTGTTCCGATTCAATCTTCCAAAAATCGAGAATCACGTTGATAATTTTca                                               >  4:440001/1‑256 (MQ=255)
                                                                                                                                                                                                                                              ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGGTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGCGCAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgt                             >  1:224663/1‑274 (MQ=255)
                                                                                                                                                                                                                                                ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAGGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATCATTTTCAACAACATGCTTGCAGAgtttt                          >  2:493846/1‑273 (MQ=255)
                                                                                                                                                                                                                                                ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGAGATGTGGTTCATCACTTCACGCGGTGATAACTAACGCGGCTCGATCTTCAACTGTTCCGATCCAACATTCGAGCAATCGCGAATCTCACTGCAATTATTCACCAACAAGCTGGacatgtttt                          >  4:581066/1‑267 (MQ=255)
                                                                                                                                                                                                                                                 gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATAGCGCTGATAATCTTCAACAACAGACTGGAAGAgttatt                         >  1:56667/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGCGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAATCCTAACTGCTTGCGTACCACCCGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACTCGGTGAAAACTCACTCGGTTCGTTCTTCCACTGTTCATATACATTATTCGAAAAATCGAAATTAGCGCTGAAAATTTTCAACAACATGCAGAAagctgtctc                         >  4:177304/1‑268 (MQ=255)
                                                                                                                                                                                                                                                   aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCCAATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTACATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCAAGAAATCGAGCATATCGCTGATAATTTTCAACAACATGCTGGAAGAGTTGTTc                        >  2:103006/1‑274 (MQ=255)
                                                                                                                                                                                                                                                        tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGc                   >  2:111436/1‑274 (MQ=255)
                                                                                                                                                                                                                                                        tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGCCGCCATTTAAGGACACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTACATAGTTTGCGGTGATGTGGTTCATCACCTCACGCGGTGAAACCGCATGCGGTTAGACGCTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATAGCGCCGATACTTTTCAACAACAGGCTGCAAGAGGTGttgcttgc                   >  1:357040/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            ttttATGGGGTTACATAACAGGTTGGTGATGA‑CCT‑GCTGTAAACGCATCGGGTCTCCATTTATGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGACTAACTGCCTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTc               <  2:287235/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                aTGGCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATGCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAAGCGGTGAAAACTCACGCGGGTCGATCTTCAACTGTTCGGATTCAATCTTCGAGAAATCGAGAATATTGCTGATAATTTCCAATAACAGGCTGGAAGAGTTGTTAATTGCACTCATCAg          >  3:320700/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                 tGGCGTTACTGAACAGGTTGGAGATTG‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAg          <  1:629750/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ggCGTTACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTAAAGCGGTTCGATCTTCAACTGTTGCGATTCAAACTTCGAGAAATTGAGAATATCGCTGATAATTTTCAACAAAAGGCTGGCAGAGTTGTTCATTGACATAAACAGcc        >  2:468820/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                      ttACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGATTTGTTAATTGCCGTCACCAGCAGAt     >  1:98721/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                      ttACTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGATTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCGGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGGCAACAGCCGaaa    >  2:646982/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                        aCTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGATCAACATTTAAGGCAACTGGCACATCCGGTTCAATACAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCGGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTAACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTAAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGa  <  1:303197/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aCTGAACAGGTTGGAGATGA‑CCT‑GCTGTAAACCCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGA‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGa  <  1:545895/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                  
CTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGA‑CCT‑GCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGC‑AGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317828‑2318366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A