Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F33 I1 R1 10 135.0 2795192 89.2% 2493311 261.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG98.4% 458.6 / NA 130L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (65/61);  minor base T (0/2);  total (67/63)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317822‑2318366
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                               
ggTAAACTGGCCGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  2:651552/276‑1 (MQ=255)
 gTAAACTGTCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAACCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  1:490108/275‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGGAGCCACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAACTCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTATACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  1:148899/275‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGGCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  1:290358/275‑1 (MQ=255)
     gcTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGGCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GCCCGGTCCCCTGGAAATTACGCTGTACGCCCGTGCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                          <  2:25817/275‑1 (MQ=255)
     aCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAgg                                                                                                                                                                                                                                                                           >  2:540012/1‑275 (MQ=255)
          cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCGCGTTCCGACCTGGAAGAAGGGTTCAAAAAAGCGCACCAATTCTTTCGCCGGTATCCCCACGCCGGTATCGCGACCACGGATAGAGAGATAATCCCCCATCC‑CGCCAACATGGCAAACTATACAGCCGGTATAGGGGAATTTTACGGCTTTACTAAACAGGTTGga                                                                                                                                                                                                                                                                      >  3:592186/1‑275 (MQ=255)
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAACGGTGCATTTTATGGCTTTACTGAACAGGTTGgaga                                                                                                                                                                                                                                                                    >  3:215251/1‑275 (MQ=255)
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTCTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGAACAAACAAGCGCACCACTTCTCTCGCCGGTATCGCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCGGCGCGAACAGTGAAAACTATAAAGCCGGGATAGGTGAATTTGATGGAGGTACTGAACAGTGTGgaaa                                                                                                                                                                                                                                                                    >  3:252925/1‑273 (MQ=255)
            gCCAATTCCCGGTTCGGAATCGACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCACCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgag                                                                                                                                                                                                                                                                     <  1:448198/274‑1 (MQ=255)
                aTTCCCGGTTCTGAATCTACCGGGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                                <  2:11392/275‑1 (MQ=255)
                 ttccccgtctagAATCCACCGAGCGATCGCCGTCCTTCATGCTGCTC‑AGGTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                                <  3:605035/263‑1 (MQ=255)
                            gAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGATCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGCAAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACCATACAGCCGGTATCGGTGAATTTTTTTGCCGTACTGAACCGGTTGGAGATTACCGTCTGTAAACg                                                                                                                                                                                                                                                    >  3:283312/1‑275 (MQ=255)
                              atcacccGAGATATCGCCGTCCATCATTCT‑ATCAAGTTTTTCACAAATCGGC‑GAGCCACGACCGGTCGCCGGGAAATTACGCGGTCCGCCCGTTCCGACCTGGGAGAAGGGATCAACCAAGAGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCa                                                                                                                                                                                                                                                  <  3:121909/270‑1 (MQ=255)
                              aTCTACCGAGATACCGCCGTCCAACATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GGCCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCa                                                                                                                                                                                                                                                  <  1:57267/275‑1 (MQ=255)
                              aTCGACCGAGATATCGCCGGCCAGCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGGCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCAt                                                                                                                                                                                                                                                 <  2:115015/276‑1 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTCCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTTTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACCGCCGGTATCGGGGAATTTTATGGCGTTACTGAACAGGTTGGAGATTACCTTGTGTaaagcaacgc                                                                                                                                                                                                                                               >  1:205252/1‑267 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAACTGCTGTAAACGCATCgg                                                                                                                                                                                                                                              >  3:75606/1‑275 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTAGCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGAGCGAACATGCAAAACTATCAAGCCGGCAGCGGGGAATTTTAGGGCGTTAATGGAAAGGTTGGGAATTACCTGCTTGAAACGCATCgg                                                                                                                                                                                                                                              >  4:78194/1‑275 (MQ=255)
                                     gggaTATCGCCGACCATCATGCTGATC‑AGGTGCTCAAAAGTCGCCAGACCAA‑GACCGGACCCCTAGAAATTACGCGGTACTCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTATGTCGCCGGTATCCCCACGGCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg                                                                                                                                                                                                                                          <  4:65791/274‑1 (MQ=255)
                                         tatCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCa                                                                                                                                                                                                                                       >  3:317728/1‑275 (MQ=255)
                                             gCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGTCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAAAGCATCGGGTCGCCATTTa                                                                                                                                                                                                                                   >  1:452169/1‑275 (MQ=255)
                                                  ccATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCCCTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGACCACGGCCAGAGAGATAAT‑CGCCATCCGACCGAACATCCCAAACAACACACCCGGTAGCGGTGCACTTTTTGCCCTTACTGAACGGGTTTGATCAGACCTGACGTAACCGCACTGTGTCCCCATTTacgcca                                                                                                                                                                                                                              >  3:641149/1‑270 (MQ=255)
                                                    atcatGCTGATC‑AGTTTTTCACAAATCGGCAGACCCA‑GGCCGGTCCCCTGGAAATTACGCGGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                             <  1:211594/274‑1 (MQ=255)
                                                        tGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTgga                                                                                                                                                                                                                        >  1:157187/1‑274 (MQ=255)
                                                             aTC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                   <  1:611052/275‑1 (MQ=255)
                                                             aTC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGAATCGGGTCGCCATTTATGGCCACTGGCACATcc                                                                                                                                                                                                                  >  2:367415/1‑276 (MQ=255)
                                                             aTC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTAGCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATAAAGCCGGTATAGGGGACTTTTATGGCGTTACTGAACAGGTTGTAAATGACCTGAGTTAACCGCGACGGGCTGCCACTTAAGGcgcgctgcccctac                                                                                                                                                                                                                  >  4:154063/1‑263 (MQ=255)
                                                              atgaGTTTTTCGCAAAGCGCCAGACCCA‑GACCGGTCCACTGGAAATGACGCTGTACGCCCGTTCCGACCTGGAATACGGGATCAAACAAGCGCACCACTTCTTTCTCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                   <  1:225616/272‑1 (MQ=255)
                                                               c‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTaaa                                                                                                                                                                                                                                                      >  3:501134/1‑238 (MQ=255)
                                                               c‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGATGACCTGCTGTACACGCATCGGGTCGCCATTTAAGCCCACAGGCACATCCgg                                                                                                                                                                                                                >  4:508355/1‑276 (MQ=255)
                                                               c‑AGTTTCTACCAAAGCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                                <  2:121283/276‑1 (MQ=255)
                                                                   tttttGACACATCGCCAGACCCA‑GACCGGGCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAG‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGtt                                                                                                                                                                                                              <  3:422654/275‑1 (MQ=255)
                                                                    ttttCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCa                                                                                                                                                                                                                                                  <  1:501134/238‑1 (MQ=255)
                                                                     tttCACAGCCCGCCCGACCCC‑GTCCGGTCCCCTTGAACTTCCGCTGTCCGCCCGTCCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTAGCGCGAACACGGATAGGGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                           <  2:611962/276‑1 (MQ=255)
                                                                             aTCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGGGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCGGTAAACGCAACGGGTCGCCATTTAAGGCCCCTGGCAAATCCGGTTAAATAAAGCa                                                                                                                                                                                                    >  1:167933/1‑275 (MQ=255)
                                                                                 caagcacca‑gaccGGTCCCCTGAGAATTCCTCGGTACGCCCGGTCCGACCTGGAAGAAAGGATCAAACACGCGCACCACGTCTGACGCCGTTCTCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAG‑CGACATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                                <  3:324849/269‑1 (MQ=255)
                                                                                  ccaggccaggaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                                <  2:492698/266‑1 (MQ=255)
                                                                                                gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTGCAATAAAGCAGTACAAGAATAACTGCTTg                                                                                                                                                                                 >  2:462618/1‑276 (MQ=255)
                                                                                                gTCCCCTGGAAATTACGCTGTACGCACGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACCCGGATAGAGAGATACT‑CGCACTCCGCGACAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGAGTTACTCAACAGGTTGGACATGACCTGCTGGAAACGCATCGGGTCGCCAATTAAGGCCCATGGAACTACAGGTTATATAAAGCATGACAAAGCTACCTGCTTg                                                                                                                                                                                 >  4:315032/1‑276 (MQ=255)
                                                                                                         aaaTTACGCATGACGCCCGATCGGAACTGGAAGAAGAGGTCAAACAAGCTCACCCCTTCATACTCAGGTATCCCCACGCCGGTACCGCAACCACGGATAGAGATATAAT‑CGCCATCCGCGCGAACATGCAAAACTTTAAAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAGAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                         <  3:403772/275‑1 (MQ=255)
                                                                                                          aaTTAAGCGGTACGCCCATTCCGACCCGGAAGAAGGGATCCAACAATCGCACTACTGCGTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGGGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                       <  4:359349/276‑1 (MQ=255)
                                                                                                           aTTACGCTGGACGCCCGTTCCGACCTGGACGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                         <  1:633468/273‑1 (MQ=255)
                                                                                                             tACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGGCACTGGCACATCCGGTTCAATAAAGCAGTAAAAGGCTAACTGGTTGCGTACCACAAGCg                                                                                                                                                                    >  2:268074/1‑276 (MQ=255)
                                                                                                             tACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGACGGTATCGTTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCTTCGGGTCGACATTTAAGGCAACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTACATGCTTGCGTACCACCAggg                                                                                                                                                                    >  4:64143/1‑274 (MQ=255)
                                                                                                               cGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATc                                                                                                                                                                                                                                                >  3:11668/1‑198 (MQ=255)
                                                                                                                 cTGTACGCCCGTTCCTACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCAGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCA‑CAGCGGTaa                                                                                                                                                                <  1:108101/275‑1 (MQ=255)
                                                                                                                  tGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTaa                                                                                                                                                                >  3:627395/1‑275 (MQ=255)
                                                                                                                   gTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                                                                                                                                                            <  1:11668/198‑1 (MQ=255)
                                                                                                                    tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATa                                                                                                                                                             >  2:675897/1‑276 (MQ=255)
                                                                                                                    tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACCGGCCCATCCGGTTCCATAAAGCAGTTCCACCCTAACTGCTTTCGTACCACCAGCCGaaaaa                                                                                                                                                              >  3:297981/1‑274 (MQ=255)
                                                                                                                          cGTTCCGACCTGGAAGAAGGAATCAAACAAGCTCACCACTTCTTGAGCCGTTATCCCCAAGCCGGTATCGCGAACACGGATTGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACGGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTgg                                                                                                                                                        <  1:363903/275‑1 (MQ=255)
                                                                                                                            ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCATTGGCACATCCGGTTCAATAAAGCAGTACAAGCATAACTGATTGCGTACCCCCAGCGGTAACGAGTTgtgg                                                                                                                                                      >  2:600618/1‑272 (MQ=255)
                                                                                                                            ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATATAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCTGTAAATCGTTGGCgg                                                                                                                                                     >  2:175805/1‑276 (MQ=255)
                                                                                                                            ttCCGACCTGGAAGAAGGGATCAAACAACCGCCCCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAAAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg                                                                                                                                                      <  1:329274/275‑1 (MQ=255)
                                                                                                                              ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTAAATAAAGAAGTACAAACCTAAACTCTTTCGTACCCCAATCGGTACATAGGTGGCggcg                                                                                                                                                   >  4:481330/1‑274 (MQ=255)
                                                                                                                                aaCCTGGAAGAACGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGa                                                                                                                                                  <  1:252925/274‑1 (MQ=255)
                                                                                                                                      gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGt                                                                                                                                                                                                               >  3:392283/1‑208 (MQ=255)
                                                                                                                                          aagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                           <  1:392283/208‑1 (MQ=255)
                                                                                                                                           agGGATCAACCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATACT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcatc                                                                                                                                       <  1:524213/275‑1 (MQ=255)
                                                                                                                                                   aaaaaGCTCACCTCTACGTTCGCCTGTACATCCTCGCCGGTATCGAGAGGATGGATAGTTAAATAAT‑GGCCATCCGCGCAAACATGCAAAACTATAAAGCCGGTATCGGTTAATTTTATGGCGTTACTGAACAGGTTGGGGATGGCCGGCAGTAAACGCATCGGGTCGCGATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCGGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTAAcg                                                                                                                               <  3:452169/272‑1 (MQ=255)
                                                                                                                                                    aCAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcgc                                                                                                                              <  2:91627/275‑1 (MQ=255)
                                                                                                                                                       aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                   >  4:44455/1‑187 (MQ=255)
                                                                                                                                                         cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGa                                                                                                                         >  2:340846/1‑275 (MQ=255)
                                                                                                                                                                      tCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGGAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAAAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGCTGTGGTTCATCACTTCACGCGGGTAAAACttcagcgg                                                                                                             >  1:124604/1‑267 (MQ=255)
                                                                                                                                                                       cGCCGATAACCCCACGCCGGAAACGCGAAACCGGATAGAGAGATAAT‑CGCCATCCGCGAGAACATGAAAAACTATACAGCAGGTATCGGTGGATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:138175/275‑1 (MQ=255)
                                                                                                                                                                       agCCGGTATACCCACGCCGGGATAGCGAACACGGAGAGAGAGATAAA‑CGCCATCCGCGCGAACATGAAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCGGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:239476/274‑1 (MQ=255)
                                                                                                                                                                        gCCGGTTACCCCACGCCGGTAGCGCGAAAACGGATAGAGAGATACT‑CTCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  3:167933/274‑1 (MQ=255)
                                                                                                                                                                        gCCGGTATCCCCCAGCCGGTATCGCGAACACGGATAGGGAGATAAT‑CGCCATCCGCGCGAACATGCAAACCTATACAGCCGGTATCGGTGAATTTGATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTACGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  1:297981/274‑1 (MQ=255)
                                                                                                                                                                             tATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACATGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCATAACTGCTTGCGTCCCACCAACGGTAAATAGTTCGAGGTAAGTTGGTTAATCCATTCACCGGGTCAAATGTCCCTCCGTTCGGTCtg                                                                                                    >  4:156465/1‑275 (MQ=255)
                                                                                                                                                                                cccaACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACGATACTGCCGGTATCGGTGAATTTTATGGCGTTACTGAACATGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:156465/266‑1 (MQ=255)
                                                                                                                                                                                caccAAGCCGGTATCGAGAACACGGATAGAGGGATAGT‑CACCATCCGCGCAAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAACCGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCaa                                                                                                 <  2:327892/274‑1 (MQ=255)
                                                                                                                                                                                     cGCCGGTAGCGCGAACACGGATAGAGAGATAAT‑GGCCATCCGCGAGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCGGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGtt                                                                                            <  2:470187/276‑1 (MQ=255)
                                                                                                                                                                                           tATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAt                                                                                       >  1:100276/1‑275 (MQ=255)
                                                                                                                                                                                           tATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGCTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTACATAGTTGGCGGTGAGTTGGTTCCTCACTTCACGTGGTGAAAACTCCCGCGGTTCGATCTTCaccctgcccct                                                                                        >  3:643525/1‑264 (MQ=255)
                                                                                                                                                                                               gcgAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  1:643525/251‑1 (MQ=255)
                                                                                                                                                                                                  aaCACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCGTTTCAGGCCACTGTCACATCCGGTTCAATACAGCCGTACAAGCATACCTGCTTGAGTACCACCAGCGGTAAAGAGTTAGCGGGTACGAGGTTCATACCTTACCGCGGTTCAAACTCAGACGGTTGCATCATCAAATGTTCCGAATCAATAtt                                                                               >  4:321708/1‑276 (MQ=255)
                                                                                                                                                                                                       ggAGAGAAAGAAAAT‑CGCCAGCCGCGCGAAAATGGAAAACTATACATCCGAGATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGGGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCgag                                                                           <  3:14195/275‑1 (MQ=255)
                                                                                                                                                                                                            gagagaTAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATc                                                                      <  1:283312/275‑1 (MQ=255)
                                                                                                                                                                                                               agaTAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATATGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCgaga                                                                  >  2:159672/1‑276 (MQ=255)
                                                                                                                                                                                                                 aTAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGCAAACTCCCGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                 >  1:319007/1‑275 (MQ=255)
                                                                                                                                                                                                                 aTAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGACAACTCACGCGGTTCGATCTTCACCTGTTCCGATTCAATTTTCGAGAAATCGATAat                                                                >  4:532798/1‑276 (MQ=255)
                                                                                                                                                                                                                 aTAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGCTCTTTAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                 >  3:538370/1‑275 (MQ=255)
                                                                                                                                                                                                                                       aTGCAAAACGATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaaca                                            <  1:592186/275‑1 (MQ=255)
                                                                                                                                                                                                                                         gCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAacaaca                                         <  2:458419/276‑1 (MQ=255)
                                                                                                                                                                                                                                          cAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATGTTCAACTGTTCCGATTAACTCTTCga                                                                            >  4:269455/1‑240 (MQ=255)
                                                                                                                                                                                                                                            aaaCTATGCAGCCGGTATCGGTGAATTTTATGGCGTTAATGAACATGTTAGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGc                                      <  4:268074/276‑1 (MQ=255)
                                                                                                                                                                                                                                             caCTATACGGCCGGTATCGGTGAATTTTATGGCGTTACTGACCAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGc                                      <  3:194319/274‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGaa                                                                         <  2:269455/240‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGc                                      <  1:75606/275‑1 (MQ=255)
                                                                                                                                                                                                                                                    cAGCCGGGATCGGTGAAGTTTACGGCGTTACTTAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAagag                              <  2:71703/276‑1 (MQ=255)
                                                                                                                                                                                                                                                       ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgt                             >  1:635703/1‑274 (MQ=255)
                                                                                                                                                                                                                                                       ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgt                             >  1:492575/1‑274 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGAAAATTTTCAACAACAGGCTgtacaagtctgt                         >  3:544665/1‑264 (MQ=255)
                                                                                                                                                                                                                                                          gTATCGGGTAATTTTATGGCGTTACTTAAACGGTTGGAGAGGACCTGCTGTAAACGCATCGGGTAGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTc                        <  4:340846/276‑1 (MQ=255)
                                                                                                                                                                                                                                                            aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTc                        >  1:484448/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTCCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCa                       >  2:143170/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACCCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTc                        >  3:602686/1‑274 (MQ=255)
                                                                                                                                                                                                                                                            aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCATAACTGCTTTCGTACCACCAGCGGTAAATAGTTGGCGGTAAGGTGGTTCATCACTTCACCCGGTGAAAACTCACGCGGTTCGATCTTCCACTGTTCCGATTCAACCTTCGAGAAATCGAGACTCACGCAGATAATTTTCAACAACCGCCGGGCAGAgttgtgc                        >  3:365039/1‑272 (MQ=255)
                                                                                                                                                                                                                                                                                tACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCTATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGAAAATTTTCAACAACATGCTGGAAGATTTGTTCATTTCCGTCACCAGCCGATcaa  >  4:230787/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                               
GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATC‑AGTTTTTCACAAATCGCCAGACCCA‑GACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAT‑CGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317822‑2318366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A