Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F34 I1 R1 10 119.3 2685262 86.6% 2325436 242.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG97.9% 336.3 / NA 97L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (50/44);  minor base T (2/0);  total (53/44)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCG  >  NC_000913/2317823‑2318365
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                               
gttgcgtGGCTGCCAACTCCCTGTTCGTGATCTTCCGAGCTATCGCCCTTCATGCAGCTGGTCTGTTATGCACGAAACGCCAGCACGTGAACGGTACCCTGAAAATTACGCTTTATGCCCGGTCCGACTTGGAGCGAGGGATCAAACCAGCGTGCCACGTGTTTCGCAGGTGTCCCCGCTCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  3:256942/269‑1 (MQ=255)
gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  3:270485/275‑1 (MQ=255)
gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGATGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  1:551924/275‑1 (MQ=255)
   aaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTCTCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCCCAACTATACA‑GCCTGTATCTTTGCATTTTATGGCGTTACTGAACAg                                                                                                                                                                                                                                                                            >  2:641018/1‑275 (MQ=255)
         cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCCCCCTCCCCGCGAAGCTGCAAAACTATACC‑GCCGGTAACGGGAACTTTTAGGGCGTTACTTAAcctgcccct                                                                                                                                                                                                                                                                      >  4:67473/1‑267 (MQ=255)
         cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTACGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCCCGCCGGTACCGCAAACACGGATAGAGAGATAAACGCCATCAGCGCGAACATGGCACACTA‑ACACGCCGGTATCGGCGGATGTTATGGGGTGAAGCGACAGGTTGgag                                                                                                                                                                                                                                                                     >  4:99311/1‑276 (MQ=255)
                ttCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAAACGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGCACAGGTTGGAGATGAcc                                                                                                                                                                                                                                                               >  2:181094/1‑275 (MQ=255)
                    ccgtacTGAATCTACCGAGCTAGCGCCGACCCTCATGCTTATCAGTTTCTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCAGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAAGTTGGAGATGAc                                                                                                                                                                                                                                                                <  1:396321/265‑1 (MQ=255)
                                 aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTTCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTAACGGTGAATTTTATGGCGTTACTTCACCGGTTGGAGATTACCTGCTGT‑AAACGCATCgg                                                                                                                                                                                                                                             >  3:255798/1‑275 (MQ=255)
                                 aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCCCCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGGATCGGTGAATGTTATGGCGTTACTGAACAGGTTGGAGATGACATCCGAT‑AAACGCacatcg                                                                                                                                                                                                                                            >  4:431222/1‑271 (MQ=255)
                                 aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACGGAACAGGTTGGATCTGACCTGCTGT‑AAACGCAGCgg                                                                                                                                                                                                                                             >  3:218370/1‑275 (MQ=255)
                                 aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGACAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑CCCGGTATAGGTGAATTTTAGGGCGTTACTGAATAGGTTGCAGATGACCTGGTGT‑AAACGCCTCgg                                                                                                                                                                                                                                             >  3:454628/1‑275 (MQ=255)
                                     agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGt                                                                                                                                                                                                                                           <  1:218370/273‑1 (MQ=255)
                                                tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAcc                                                                                                                                                                                                                                                               >  2:401980/1‑243 (MQ=255)
                                                    tcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCTACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAAc                                                                                                                                                                                                                                                                              >  1:43969/1‑224 (MQ=255)
                                                            aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCTAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATACGCGCGAACCTGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTTGAAATGAACTG‑TGTGAAACGCATCGGGTCGCCATTTAAGGACACTGGccacacc                                                                                                                                                                                                                 >  4:369129/1‑270 (MQ=255)
                                                            aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                  >  1:175281/1‑275 (MQ=255)
                                                            aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTAGGGCGTTACTGAACAGGTTGTAGATGACCTGCTGT‑AAACGCAACGGGTAGCCATTGAAGGCCACTGGCACATct                                                                                                                                                                                                                 >  2:293805/1‑275 (MQ=255)
                                                              cAGTTCTGCACAAATCGCCCGACCCAGACCCGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                               <  2:431222/276‑1 (MQ=255)
                                                               aGTTTTTCACAAACCGCCAGACCCAGACCGGTCCCCAGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                               <  2:707057/275‑1 (MQ=255)
                                                                 tttttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTACTGT‑AAACGCATCGGGTCGCCATTTCAGGCCACTGGCCCATACGGTTc                                                                                                                                                                                                            >  2:619899/1‑276 (MQ=255)
                                                                        taagagacaggacccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                     <  2:63560/266‑1 (MQ=255)
                                                                          aaccGCCAAACCCAGACCGAGCCCCTAGAAATTCCGCTGTCCGCCCTTTCCGACCTGGAAGAAGGGATCAAACAACCTCACCACGTCGTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAAAGATAATCTCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                     <  2:316847/271‑1 (MQ=255)
                                                                          aaaagacagacccagaccGGTCCCCTAGAAATTACGGTGTACTCCCTTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                     <  3:374720/268‑1 (MQ=255)
                                                                              gccaccaccagacgGGCCCCCTGGAGATTAGGCTGCACGCCCGTCCCGACCTGGAAGAAGGGATCAATCAAGAGCACAGCTTCTTTCGCCGGTATCCCCCCGCCGTTATCGCGAGCACAGAGAGAGAGATAATCGCCATCCGCGCGAACATGCAAACCTATACA‑TCCGGTATCGGTGAATTTTATGGCGTTACTGAGCAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  4:293805/269‑1 (MQ=255)
                                                                               ccagacccaggcCGGACCCCTGGAAATTCCGCTGTCCGCTCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCCCTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGt                                                                                                                                                                                                 <  4:459583/273‑1 (MQ=255)
                                                                               ccagaccaagacCGGTCCCCTGGAAATTCCGCTGTACACCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGATAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  2:286965/275‑1 (MQ=255)
                                                                                agggacaggaccGGGCCCCGGGAAATTACGCTGTACGCCCGTTCCGACCGTGAAGAAAGGATCAAACAACCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  3:116529/266‑1 (MQ=255)
                                                                                   acccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACCAGc                                                                                                                                                                                           >  1:604909/1‑275 (MQ=255)
                                                                                       agaccGATCCCCTGGAAAACACAGTTGCCGCCCGTTCACACATAGAAAAAGAGATCAAACAAGCGCACCACTACTTCAGACGGGAACCCCAAGCCGGTATCGCGAACACGGAGAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAAc                                                                                                                                                                                      <  4:181094/276‑1 (MQ=255)
                                                                                        gaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCAACGGGTCGGCATTTAAGGCCAATGGCCAATCCGGGTTAATAAAGCAGTAAAAGACTAAc                                                                                                                                                                                      >  3:227149/1‑275 (MQ=255)
                                                                                          ccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCTAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGc                                                                                                                                                                                   <  2:369129/276‑1 (MQ=255)
                                                                                            ggTCCCCTGGAAATTACTCTGGACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCt                                                                                                                                                                                  <  1:56257/275‑1 (MQ=255)
                                                                                            ggTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  1:227149/262‑1 (MQ=255)
                                                                                             gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGG‑AAACGCCTAGGGTCGCCATTTACGGCCACGGGCCAATCCGGTTAACAAAAGCCACACAAGcactgtcccgta                                                                                                                                                                                >  4:63560/1‑265 (MQ=255)
                                                                                              tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGccctgtctcct                                                                                                                                                                                 >  1:374720/1‑265 (MQ=255)
                                                                                              tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTTACTGCTTg                                                                                                                                                                                >  1:107570/1‑275 (MQ=255)
                                                                                              tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGAATCGGGTCGCCATTTAAGGCCACGGGCCCATCCGGTTCCATAAACCCGACCCAGCCTAAATGGTTga                                                                                                                                                                               >  4:134346/1‑275 (MQ=255)
                                                                                                   tGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCCCATCCGGTTCAATAAAGCa                                                                                                                                                                                                   >  1:116529/1‑251 (MQ=255)
                                                                                                       aaTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                      <  2:134346/276‑1 (MQ=255)
                                                                                                       aaTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCGTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                        <  2:679715/274‑1 (MQ=255)
                                                                                                           aCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTAACGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCAACTGGCACATCCGGTTACATAAAGAAGTACAAGCCTAACTTCTTGCGTACAACCCGCgg                                                                                                                                                                  >  4:454984/1‑276 (MQ=255)
                                                                                                             gCTGTAAGCACGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCCCCACTTCTTTCCCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGt                                                                                                                                                                 <  2:371190/275‑1 (MQ=255)
                                                                                                                 tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATTCA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTTGCACATCCGGTTCAATAAAGCAGTACCAGCCTAACTGCTTCCGTACCAACAGCGGTacaga                                                                                                                                                            >  4:464038/1‑272 (MQ=255)
                                                                                                                 tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAACGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATa                                                                                                                                                            >  2:378910/1‑276 (MQ=255)
                                                                                                                   cGCCCGTTCCGACCTGGAAAAAGGAATCAACCAAGCGCCCCACTTCTTTCGCCGTTATCCCCCCCCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                          <  2:99311/276‑1 (MQ=255)
                                                                                                                                   gaagaaGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTCATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAATCCTAACGGCTTGCGTACCATCAGCCTGAAATAGTTGGCCgtggtgtggt                                                                                                                                           >  3:100387/1‑275 (MQ=255)
                                                                                                                                             tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCTATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGCGATGACCTGCTGT‑AAACGCATCTGGTCGCCATTTAAGGCCACTGGACCATCCGGTTCAATAAAGCAGTACAAGTCTAACTGCTTGCTTACCACCAGCGGTAAGTCCTTGCCGGTGATGTGGTTATTCATTTc                                                                                                                                 >  4:193018/1‑275 (MQ=255)
                                                                                                                                             tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACCGAACAGGTTGGAGATGACCTGCTGT‑AAACTCATCGGGACGCCCTTTAAGGCCCCTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAAATGCTTGGGTACTACCCACGGGAAATAGTTGGCGGTGATGGTGTTTATTACtt                                                                                                                                  >  3:622494/1‑274 (MQ=255)
                                                                                                                                                 aCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GTCGGTACTGGTGAATGTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACCTCCGTTTCAATAAATCAGTACCAGCCTCACTGCTTGCGTCCCCACAGCGGTAAATAGTCGGGAGGCATGTGGGTCATCACttaatac                                                                                                                             >  4:580857/1‑270 (MQ=255)
                                                                                                                                                 aCAAACGCACCACGTCTTGCGCCCGTGTCCCCGCGCCGGTAGCGCGAACACGGATAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑TCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACGGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcg                                                                                                                              <  1:454628/274‑1 (MQ=255)
                                                                                                                                                              tgcttTCGCCGGTATCCCCACGGCGGTATCGCGAACACGGATAGGGAGATAATAGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAc                                                                                                                <  3:80558/273‑1 (MQ=255)
                                                                                                                                                                    cGCCGGTTTCCCCACGCCGGTATCGCGGACACGGATAGAGAGATAATCGCCATCCGCGCCAACATGCAAAACTAGTCG‑GCCGGTATCGGTGAATTTTATGGCGTTAATGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAGAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  2:580857/275‑1 (MQ=255)
                                                                                                                                                                    cGCCGGTCACCCCAGGCCGGTAACGCGCACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACG‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  3:107570/275‑1 (MQ=255)
                                                                                                                                                                    cGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  2:454984/275‑1 (MQ=255)
                                                                                                                                                                     gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAg                                                                                                                                                           >  3:368720/1‑225 (MQ=255)
                                                                                                                                                                     gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  1:255798/274‑1 (MQ=255)
                                                                                                                                                                     gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTCCGTACCACCAGCGGTAAATAGTTGTCGGTGATGTGGTTCATAACTTCACGGGGTGAAAACTCACGCGGtt                                                                                                          >  1:556619/1‑274 (MQ=255)
                                                                                                                                                                         gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTgg                                                                                                                                                       <  1:368720/225‑1 (MQ=255)
                                                                                                                                                                         gTAACCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                     <  3:175281/275‑1 (MQ=255)
                                                                                                                                                                                   gCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCa                                                                                                                                                                                                   >  1:272268/1‑171 (MQ=255)
                                                                                                                                                                                   gCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcgc                                                                                                              >  3:116877/1‑256 (MQ=255)
                                                                                                                                                                                       gTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  3:272268/171‑1 (MQ=255)
                                                                                                                                                                                       gTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  1:116877/256‑1 (MQ=255)
                                                                                                                                                                                        tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAtt                                                                                     >  2:263758/1‑276 (MQ=255)
                                                                                                                                                                                         aTCGCGACCACGGAAAGAGAAATAGTCGCCATCCGCGCGAACATGCAAAACTGTCCG‑GCCGGTATCGGTAAATTTTATGGCGGTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAtt                                                                                     <  1:16670/275‑1 (MQ=255)
                                                                                                                                                                                         aTCGCGAACACGGATAGAGGGATATTCGCCATCCGAGCGAACATGCAAAACTTTACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCAGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAtt                                                                                     <  1:100387/275‑1 (MQ=255)
                                                                                                                                                                                             cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCg                                                                                                        >  2:535254/1‑252 (MQ=255)
                                                                                                                                                                                                acacGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                     <  4:535254/252‑1 (MQ=255)
                                                                                                                                                                                                          agagaTAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTAGCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGACAACTTACGCGGTTCGATCTTCAACTGTTCCGAGTCACTCTTCGAAAAATCg                                                                    >  3:73630/1‑275 (MQ=255)
                                                                                                                                                                                                            agaTAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCgag                                                                  >  3:623639/1‑275 (MQ=255)
                                                                                                                                                                                                              aTAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcgcg                                                                                                             >  4:207198/1‑230 (MQ=255)
                                                                                                                                                                                                                aaTCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgaga                                                                                                                                                                                                                                                                    >  1:399366/1‑78 (MQ=255)
                                                                                                                                                                                                                aaTCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGAAAATAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGGTGAAAACCAACGCGGTTCGCTCTTCAACTGTTCCGATTCCATCTTCCAGCAATCGAGAatat                                                             >  4:115375/1‑276 (MQ=255)
                                                                                                                                                                                                                 aTCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  2:207198/230‑1 (MQ=255)
                                                                                                                                                                                                                  tCGCCATCCGCGCGAACATGCAAACCTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                               <  1:242985/65‑1 (MQ=255)
                                                                                                                                                                                                                    gCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                                <  3:399366/78‑1 (MQ=255)
                                                                                                                                                                                                                    gCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTTAAAACTCACGCGGTTCGTTCTTCAACTGTCCCGATTCAACCTTCGAGAAATCGAGAatagccc                                                          >  3:206249/1‑271 (MQ=255)
                                                                                                                                                                                                                      cATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTCACTGCTTGCGTACCACCAGCGGTAAATCGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGAGGTTCGGTCTTCAACTTTTCCGATTCAATCTTCGAGAAATCGAACATATCGCTg                                                        >  3:515651/1‑275 (MQ=255)
                                                                                                                                                                                                                      cATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTg                                                        >  1:21930/1‑275 (MQ=255)
                                                                                                                                                                                                                        tCCGCGCAAACATGCAAAACTATACA‑GCCGGTATCGGTGGATTTTATGGCGTTACTGAACAGGTTGTAGATGACCTGCTGT‑AAGCGCTTCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcatc                                                                                                                                      <  1:521094/195‑1 (MQ=255)
                                                                                                                                                                                                                         ccGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTACGGCCACTGGCACATCCGGTTCAATAAAGCACTACACGCCTAACTGCTTGCGTACCCCCAGCGGTATATAGTTGGTGGTGAGTTGGTTCCTCCCTTCACCGGGTGGAAACTCCCTGGGTCCGACATTCCACGGTTCCGATTCATTCTTCGAGAAATCGAGAATCTCGCTgcaa                                                     >  3:41446/1‑272 (MQ=255)
                                                                                                                                                                                                                            cgcgAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATa                                                     >  1:499601/1‑272 (MQ=255)
                                                                                                                                                                                                                                               taaggCCGGTATCGGTGAATGTTATGGCGTTACTGAACAGGTTGGGGATGAACAGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTGCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGaa                                <  3:556619/271‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCCAGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCCGAAAATCGAGAATATCGCTGATACTTTTTAACAACCggagtgacgctt                            >  4:696094/1‑264 (MQ=255)
                                                                                                                                                                                                                                                    ccGGTATCGGTGAATTTGATGGCGTTACTGAACAGGATGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACTTCCGGCTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGGATATAGTTGGCGATGAAGGTGTTCATCACTTCACGCGGTGAACACTCACGCGGTGCGATCTTCAACTGTCCCAATTCACTCTTCGTGAAATCAAGAATATTGCTTTTAATTTTTAACACCAGCACGGAAGAgttg                          >  4:617666/1‑276 (MQ=255)
                                                                                                                                                                                                                                                      ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcgc                                                                                                              >  3:625881/1‑190 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGTTCTTCAACGGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCCGATAATTTTCAACAACAGACCGGAAGAgttgtt                        >  3:74780/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCgaga                                                                         >  1:45265/1‑226 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                          <  2:696094/257‑1 (MQ=255)
                                                                                                                                                                                                                                                         aTCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCCGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGAGCTTCAACTTTTCCCATTCAATCTTCGACAAATCGTGAATCACCCTGATAATTTTCAACAACCTGCTGGAAGAGTTGTTCa                      >  4:539396/1‑275 (MQ=255)
                                                                                                                                                                                                                                                          tCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  1:625881/190‑1 (MQ=255)
                                                                                                                                                                                                                                                           cGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATc                                                                     <  3:45265/226‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTc                                                                             >  2:90647/1‑211 (MQ=255)
                                                                                                                                                                                                                                                                     tATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCgag                                                                          <  4:90647/211‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ttACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCTACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTAACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCCAGAAATCGAGAATCTCGCTTATAATTTTCAACAACAGGCTGGCAGAGTCGTTAATATCCGCCACAACCCgct    >  3:196773/1‑272 (MQ=255)
                                                                                                                                                                                                                                                                             tACTGAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCg  >  1:198358/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                               
GTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA‑GCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGT‑AAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCG  >  NC_000913/2317823‑2318365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A