Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F70 I1 R1 11 132.3 2683406 92.3% 2476783 251.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG97.5% 441.8 / NA 121L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (57/61);  minor base T (2/1);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317822‑2318366
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                  
ggTAACCTGGCTGGCCCTTCCCGGTTCTGAATCCACCGAGATATCGCCGACACTCATGCTGATCAGTTTTGCCCAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  4:421075/276‑1 (MQ=255)
ggTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGTTGATCAGCTTTTCACAAATCGCCAGACTCAGACCGGTACCCTGGAAATTAAGCTGTACGCTCGTTCCGACCTGGAAGAAGGGTTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACTGATAGAGAGATAAGCGCCACCCGCGCGAACACGCCAAACTTGACAGCCGGTATCGGGGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  >  2:34255/1‑276 (MQ=255)
 gtcaaCTGGCTGCCCAATCCCGGTTCTGAATCTACCCAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  3:111448/272‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  1:651532/275‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  1:75963/275‑1 (MQ=255)
     acgggCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGAGCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCGGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  2:570885/273‑1 (MQ=255)
     acgggCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGGCCATAATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  4:609759/273‑1 (MQ=255)
     aCTGGCTGCCCAGTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCAAGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCACGTTCCGACCTGGAAGAAGGGATCAAACAAGGGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  2:541882/276‑1 (MQ=255)
      cgggCTGACCAATCCCGGTTCTGGATCTACCGCGATATCGCCGGCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTGCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGAGAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg                                                                                                                                                                                                                                                                              <  1:552258/272‑1 (MQ=255)
      cTGGCTGCCCATGCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCCGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  1:571570/275‑1 (MQ=255)
      atagCTGCCCTATCCCGGTTCTGACACTACCGCGATATCGCCGTCCATCATGCTGATCAGTTTTTCCCCAATCGCCAGACCCAGACCGGGCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                             <  3:171422/272‑1 (MQ=255)
          cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAACCTATACAGCCGGTAGCGGTGAATTTTATGGCGTTACTGAACAGGTTGga                                                                                                                                                                                                                                                                         >  1:136246/1‑275 (MQ=255)
                 gtCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATTCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                                   <  1:660693/273‑1 (MQ=255)
                        ttCTGAATCTACCGCGATATCGCCGTCCATCATGCTGATCAGGTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAGTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑t                                                                                                                                                                                                                                                           <  1:21256/274‑1 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGgaga                                                                                                                                                                                                                                                                       >  3:560944/1‑253 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCgg                                                                                                                                                                                                                                                >  3:428148/1‑275 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATCAGATTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACACGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGCACATGCAAAACTATACAGCCGGTATCGGTGCATTTTATGGCGTTACTGAACAGGTTGGCGATGACCTGCTG‑TACACGCCTCgg                                                                                                                                                                                                                                                >  2:475443/1‑275 (MQ=255)
                                    cGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCGG‑TCAACGCATCGGGTc                                                                                                                                                                                                                                             >  2:275818/1‑276 (MQ=255)
                                      tgaTATCGCCGTCCAGCATGCTGATCAATGTCTCACAAACCGCCAGACCCAGACCGGTCCCATGGAAATTACGCTGTACGCCCGTTCCGACCAGGAAGAAGGGATCAAACAAGCGCACAACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAGACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCg                                                                                                                                                                                                                                            <  2:623101/274‑1 (MQ=255)
                                              ccGACCATCATTCTGGTCAGTTTTTCACAAGTCGCCAGACCCAGACAGTGCCCCTGGAAATTACGTTCTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGGACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTaa                                                                                                                                                                                                                                    <  3:574313/275‑1 (MQ=255)
                                                gTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAGTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  2:486075/228‑1 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCCAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTGACTGAACAGGGTGGAGATGACCTGCTG‑TAACCGCACAGGGTCGCCATTTAAGGc                                                                                                                                                                                                                                 >  3:357414/1‑275 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGc‑‑c                                                                                                                                                                                                                              >  2:536880/1‑276 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTACCGCGAACACGGCTAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACGGCCGGTATCGGTGAATTTTATGGCGTTACTGACAAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGc                                                                                                                                                                                                                                 >  3:259370/1‑275 (MQ=255)
                                                  ccATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAATTTCCGCTTTACGCCCGTTCCGCCCTGGAAGAAGGGATAAAACAAGCGAACCACTCTTTCCGCCGGTATCCCCACCCGGGTCTCCCGAACCCGGAAAGGAAGAAAATCGCCATCGCCGCGAACATGCAAACATACACAGCCGGAACCGGGGATTTTTATGCGGTTACTGACAAGGTTGGAGATG‑CCCGCTGTTAAACGCACCGGTTCCCCATTTAAgtca                                                                                                                                                                                                                                >  3:483540/1‑272 (MQ=255)
                                                   catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑Ca                                                                                                                                                                                                                             <  3:666833/275‑1 (MQ=255)
                                                       atGCTGCTCAGTTTTTCCCAAACCCCCAGGCCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGc                                                                                                                                                                                                                        <  2:439559/276‑1 (MQ=255)
                                                             aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATc                                                                                                                                                                                                                   <  3:260368/275‑1 (MQ=255)
                                                             aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATc                                                                                                                                                                                                                   <  1:428148/275‑1 (MQ=255)
                                                             aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATAGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑GAAACGCATGGGGTCGCCGTTTAAGGC‑‑CACTGGcacaac                                                                                                                                                                                                                   >  3:525451/1‑273 (MQ=255)
                                                               cAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTTCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CCCTGGCACATCCg                                                                                                                                                                                                                 >  3:195195/1‑275 (MQ=255)
                                                               cAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCgg                                                                                                                                                                                                                <  2:435965/276‑1 (MQ=255)
                                                               cAGTTTGTCAAAAAGTGACCGACCCATCACGGTCCCCTGGAAAGTACGCCGTCCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCCCCACTTCTTTCGCCGGTAGCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCAGCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCTTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCgg                                                                                                                                                                                                                <  2:580903/276‑1 (MQ=255)
                                                                  tttttCACGAAACCCCAGCCCCAGACCGGTCGCCTGGAAATTACACAGGACGCCCGTTACGACCTGGAAAAAGGGATCAAACGACCGCACCACTTCTTTCGCCGGTAGCCCCACGCCGGTATCGCGAACACGGATAGAGAAGTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCTTCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGtt                                                                                                                                                                                                              <  1:483540/275‑1 (MQ=255)
                                                                      tcccaAATCGCCAGACCCCGACCGGTCCCCTGGAAATTACGCTTTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATa                                                                                                                                                                                                         <  2:638404/273‑1 (MQ=255)
                                                                       cacaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                                  <  4:127709/205‑1 (MQ=255)
                                                                       cacaAATCGCCAGACCCAGACCGGTCACCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGAACAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGGGTTGACCTGCTG‑TAAACTCATAGGGTCGCCCTTTACGTC‑‑ACATGGCCCATCCGGTTCAAt                                                                                                                                                                                                          >  2:286957/1‑274 (MQ=255)
                                                                         caaacggccatccccagaccTGTCCCCGGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAAAACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                      <  2:532641/264‑1 (MQ=255)
                                                                                ccagacccatccCGGTCCCCTGGAAATTACGCGGTACGCCCGTTCCGACCTGGAAGAATGGAGCAAACAAGCACACCACGTCTTGCGCCGTGATCCCCACGCCAGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                                <  1:240771/275‑1 (MQ=255)
                                                                                              gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCtt                                                                                                                                                                                  >  1:177338/1‑275 (MQ=255)
                                                                                               tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGCAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACAAGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAAGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCCCCATTAAAGGC‑‑CACAGGCACAACAGGTTCAATAAAGACGTACAAGCCaaaagtcccgg                                                                                                                                                                                >  4:477606/1‑268 (MQ=255)
                                                                                                ccccTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCGATTTAAGGC‑‑CCCTGGCACATCCGGTGCAATAAAGCAGTACAAGCCTAACtgcgtgcg                                                                                                                                                                               >  2:34674/1‑276 (MQ=255)
                                                                                                ccccTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGGTCAATAAAGCAGTACAAGCCTAACTGCTTGCg                                                                                                                                                                               >  2:395066/1‑276 (MQ=255)
                                                                                                 cccTGGAAATTACGCTGTCCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCg                                                                                                                                                                               <  2:489432/275‑1 (MQ=255)
                                                                                                      gAAATTACGCGGTAAGCCCGTACCGACCTGGAAGAAGGGAGCAAACAAGCGAACCACTCCTTTCGCCGGTATCCCCACGCCGGTATCGCGAAACCGGATAGAGAGATAATCGCCATCCGCGCGAACATGAAAAACTATACAGCTGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                         <  4:286957/276‑1 (MQ=255)
                                                                                                          ttacacggagcGCCAGTTCCGAACTAGCATAAGGGAATAAACAAGCTCACCCCTTCTTTCGCCGGTATCCCCACGCCGGGATCTCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGTTATCGGTGAATTTTATGGCGTTACTGAACAGTTTGGTGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGCCACACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                       <  4:628266/266‑1 (MQ=255)
                                                                                                                          ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTGCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg                                                                                                                                                      <  1:357414/275‑1 (MQ=255)
                                                                                                                                caggggagaatGGATCAAACAACCGCACCACTTCTTGCGCCGGTATCCCCACGCCTGATTCTCGAACACGGATAGGGAGTTAAGCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCGATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtg                                                                                                                                                <  4:475443/269‑1 (MQ=255)
                                                                                                                                   ggAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAGCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑t                                                                                                                                                                                                                                                           >  2:249922/1‑167 (MQ=255)
                                                                                                                                      agaaaGGATCAAACAAGCGCACCACTTCTGTCGCCAGTATCCCCACACCGGTATCGCGAACCAGAATGGAGAGATAACAGCCATCCTCGCGAACATGCAAAACAATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtca                                                                                                                                         <  2:290070/276‑1 (MQ=255)
                                                                                                                                          gggATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑AACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATCGTTTGCGGAGATGTGGTtcatca                                                                                                                                      >  3:75959/1‑275 (MQ=255)
                                                                                                                                              tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCCCGAACACGGATAGAGAGATAATAGCTATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTTCATTTTATGGCGTTACTGAACAGGGGGGAGATGACCTCCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑TACTGGTACCTCCGGTTCAATAAAGCAGTACACGCCGAACGGCTTGCGTACGACCCGCGTGCACTAGATAGCGTGGATGTGGCtcatccattc                                                                                                                                  >  3:152566/1‑270 (MQ=255)
                                                                                                                                                 aaCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcg                                                                                                                               <  1:368985/275‑1 (MQ=255)
                                                                                                                                                     agcgcgcaccttcttTCGACTGTATCCCCCCGCCGGTATCCCGAACACGGATATAAGAATAATCGCCATCCGCGCGTACATGCAAACGTATACAGCCGGTATCGGTGAATTTTATTGCGTTACTGAACAGGTTGGAGATGACCTGCTT‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAACGGTAAATAGTTGACGGTGATGTAGTTCGTCACATCACGCGGTg                                                                                                                          <  4:34255/267‑1 (MQ=255)
                                                                                                                                                      gcgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCACCATCCGCGCGAACATGCAAAACTATACAGCGGGTATCGGTGAATTTTATGGCGATACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTCCAATAAAGCAGTACAAGCCTAACTGCCTGAGTACCACTAGCGGTAAATGGTTGGCGGTGATGGGGATCATCCCTACACGCGGTGa                                                                                                                         >  2:158496/1‑276 (MQ=255)
                                                                                                                                                       cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTg                                                                                                                          >  3:623076/1‑274 (MQ=255)
                                                                                                                                                                    tcccctgTATCCCCCAGCCGGTAACGCGGAAACGGAGAGAGAGATAATCGCCATCCGCGCGAACATGAAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  4:275818/270‑1 (MQ=255)
                                                                                                                                                                    tCGCCGGTATCCCCCGGCCGGAACCGCGAAAACGGGTAAAGAAGTAATCCCCATCCGCGCGAAAATGCAAAACAATCCAGCGGGTATCGGTGAATTTTATGACGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCAAATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  2:477606/276‑1 (MQ=255)
                                                                                                                                                                      gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATACTTGGCGGTGGTGTGGTTCCTCACTTCACCCGGTGaaaaacaactggggtg                                                                                                          >  4:646214/1‑263 (MQ=255)
                                                                                                                                                                      gCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCCG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTAAATAAAGCAGTACAAGCCTAACTGCTTTCGTACCACCCGCGGTAAATAGTTGGAGGTGATGTGGTTCAGCACTTCAAGCGGGGAAAACTCACGCGGTTc                                                                                                          >  4:593494/1‑275 (MQ=255)
                                                                                                                                                                      gCCGATAGCCCCACGCCGGTAGCGAGAACACGGATAGAGAGATAATCGCCATCCGCGCAAACATGAAAAACTATACAGCCGGTATCGTTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAGAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                           <  1:525451/274‑1 (MQ=255)
                                                                                                                                                                      cccGGCACCCCCACGCCGATATCTCGAACACGGATAGAGAGATAATCGCCAGCCGCGCGAACATGCAAAACTATACAGCCGGTATCGTTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCg                                                                                                         <  2:262521/275‑1 (MQ=255)
                                                                                                                                                                          gTATCCCCAGGCCCGTAACGCAAACACGGAAAGACAGATATTCGCCCTCCGTCGGAACATCAAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAGCTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                      <  3:180775/275‑1 (MQ=255)
                                                                                                                                                                           tATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAAt                                                                                                                                                                                                          >  1:67564/1‑174 (MQ=255)
                                                                                                                                                                               cccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                      <  3:67564/174‑1 (MQ=255)
                                                                                                                                                                                         tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCTTCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGAt                                                                                       >  1:408127/1‑275 (MQ=255)
                                                                                                                                                                                         tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAAc                                                                                                >  3:171907/1‑266 (MQ=255)
                                                                                                                                                                                         tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTCAAATAAAGCAGTACAAGCATAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGGGGATCATCAGTTCGCGGGGTGAAAACTCACGCGGGTAGACCTGCAACCGtgtcggtg                                                                                      >  4:195880/1‑269 (MQ=255)
                                                                                                                                                                                             gcgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGtt                                                                                            <  1:171907/266‑1 (MQ=255)
                                                                                                                                                                                              cgAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTAATCACTTCACGCGGTGAAAACTCACGCGGTTCGCACTTCAACTGTTCCGATTCAAt                                                                                  >  1:62496/1‑275 (MQ=255)
                                                                                                                                                                                                    cGGATAGAGAGTAAATCGCCATCCGCGCGAACAGCAAAAACTATCCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCgag                                                                           <  2:99611/276‑1 (MQ=255)
                                                                                                                                                                                                             agaTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGAACTTCAACTGTTCCGATTCAATCTTCGAGAAATagag                                                                   >  1:299720/1‑275 (MQ=255)
                                                                                                                                                                                                                 aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTACGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACACACGCGGTTCGATCTTCAACTGTTCCGCTTCAATCGTAGTGAAATCGACActat                                                              >  2:503839/1‑276 (MQ=255)
                                                                                                                                                                                                                 aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGGGGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTAGAGAAATCGAGaac                                                                >  1:99784/1‑273 (MQ=255)
                                                                                                                                                                                                                         tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAGTTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGTAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCCTCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGAt                                                       >  2:154668/1‑275 (MQ=255)
                                                                                                                                                                                                                             cgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGcac                                                                                                                                                                                                                      >  2:466499/1‑112 (MQ=255)
                                                                                                                                                                                                                                gAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATc                                                                                                                                                                                                                   <  4:466499/112‑1 (MQ=255)
                                                                                                                                                                                                                                 aaCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCgg                                                                                                                                                                                                                <  1:53280/114‑1 (MQ=255)
                                                                                                                                                                                                                                    acgaaaaaCTATACAGCCGGTAGTGGTGAATTTTATGGCGTTACTGAACAAGTTGGAGATCACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCGTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                           <  4:475256/272‑1 (MQ=255)
                                                                                                                                                                                                                                    aTGCAAAAATATACAGAAGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGTTGACCTGCTG‑TAAGCGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATTGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                           <  2:343165/276‑1 (MQ=255)
                                                                                                                                                                                                                                    aTGAAAATCTATTCCGGCCGTACCGGTGAATTTTATGGCGGTACTTAACCGGTTGGGGATGACCTGCTG‑GAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                           <  4:395066/276‑1 (MQ=255)
                                                                                                                                                                                                                                               taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACt                                                                                                                    >  1:600698/1‑192 (MQ=255)
                                                                                                                                                                                                                                                   gCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcg                                                                                                                <  3:600698/192‑1 (MQ=255)
                                                                                                                                                                                                                                                    ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTg                                                                                                                                                                                     >  3:453557/1‑122 (MQ=255)
                                                                                                                                                                                                                                                    ccGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATAACTTCACGCGGTGAAAACTCACGCGGTTCAATCTTAAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACACCAGGCTGGACGAttt                            >  2:157474/1‑275 (MQ=255)
                                                                                                                                                                                                                                                      ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgt                          >  3:300413/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcg                                                                                                                <  4:50775/188‑1 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttgt                          >  1:96068/1‑274 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGCACGGAAGAgttgtt                         >  1:255747/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAAACGGCTGGAAGAgttttt                         >  3:278456/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACCCACGCGGTTCGATCTTCCACTGTTACGATTCAATCTTCGAGAAATCGAGACTATCGCTGATAATTTTCAACAACAGGCTGGAAGAgtgttt                         >  1:518152/1‑271 (MQ=255)
                                                                                                                                                                                                                                                       gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGAGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTAACGCGGTGAAAACTCACGCGGTTCGAGCTTCAACTGTTTCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTAAACAACAGGCTGGAAGAgttgtg                         >  1:238461/1‑274 (MQ=255)
                                                                                                                                                                                                                                                        tATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTg                                                                                                                                                                                 <  1:453557/122‑1 (MQ=255)
                                                                                                                                                                                                                                                         agcGGTGAATTTTATGGCCGTACTGAAACGGTTTGAAATGGCCTAGTG‑TAACCGCATCGGGTCGCAATTTAAGGC‑‑CACTGGCGCATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCa                       <  1:639745/273‑1 (MQ=255)
                                                                                                                                                                                                                                                              tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGGGTTGTTAATTGcct                 >  2:441841/1‑275 (MQ=255)
                                                                                                                                                                                                                                                              tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTCAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGcc                  >  2:47028/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                            ttACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGCAATCGAGAATATCGTTGATAATTTTCAACAACAGGCTGGAATAGTTGTTCATTGCCGTCACCATCCGATc    >  1:124469/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                            ttACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATc    >  1:392405/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                            ttACTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTAACCAGCAGacc    >  3:497776/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                              aCTGAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGa  <  2:107499/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                  
GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTG‑TAAACGCATCGGGTCGCCATTTAAGGC‑‑CACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGA  >  NC_000913/2317822‑2318366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A