Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F104 I1 R1 10 127.5 2652057 91.2% 2418675 257.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG99.1% 403.9 / NA 114L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (62/51);  minor base T (0/1);  total (62/52)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 114 total aligned reads displayed.

GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCG  >  NC_000913/2317822‑2318365
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              
ggcaaccatccaccccAATCCCTGTTCAGAATCTCCGGGAATATCGCCGTCCATCATGCTGGTCAGTTTTACACAAAGCGCCAGACCCAGACCGGTCCCCTGGAAAGTACGCTGTCCTCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCGCGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGaa                                                                                                                                                                                                                                                                              <  4:577369/263‑1 (MQ=255)
cgtaatttGGCTGCCCATTCCCGGTTCTGAATTTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTGCACAAATCGCCAGACCCAGACCGCTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTTGAAGAATGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATTGCGAACACGGATAGAGATATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCCGTATCGGTGAATTTTATGGCGT‑TACTGaa                                                                                                                                                                                                                                                                              <  2:47802/269‑1 (MQ=255)
 gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAACGCCATCCGCGCGAACATGCAAAACTATACAGACGGTATCGGAGAATTTTATGGCGT‑TACTGaa                                                                                                                                                                                                                                                                              >  2:526345/1‑275 (MQ=255)
          cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTAGGGCGT‑TACTGAACAGGTTGga                                                                                                                                                                                                                                                                     >  1:623036/1‑275 (MQ=255)
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCAAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGGGAATTTTATGGCGT‑TACTGAACAGGTTGgaga                                                                                                                                                                                                                                                                   >  1:242132/1‑275 (MQ=255)
            gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCAATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGACCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATAAAGCCGGTATCGGTGAATTTTATGGCGT‑TTCTGCACAGGTTGgag                                                                                                                                                                                                                                                                    >  1:384926/1‑274 (MQ=255)
                aTTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCCGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGATATAATCGCCACCCGCGCGACCATGCAAAACTACACCGCCGGTATGGGGGAATTTT‑TCGCATGTACTGACACGGTTGGAGATGAcc                                                                                                                                                                                                                                                              >  4:57042/1‑276 (MQ=255)
                       gTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACCGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACctgcttt                                                                                                                                                                                                                                                        >  2:468653/1‑273 (MQ=255)
                       gTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACGGAACAGGTTGGAGATGACCTGCTGTa                                                                                                                                                                                                                                                       >  2:413677/1‑276 (MQ=255)
                       gTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAGCGGTGAATTTTATGGCGG‑TACTGAACAGGTTGGAGATGAACTGCTGt                                                                                                                                                                                                                                                        >  1:355578/1‑275 (MQ=255)
                            gAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TCCTGAACAGGTTGGAGATGACCTGCTGTAAACGc                                                                                                                                                                                                                                                  >  2:40719/1‑276 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGaa                                                                                                                                                                                                                                                                              <  3:68241/242‑1 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGaa                                                                                                                                                                                                                                                                              >  1:68241/1‑242 (MQ=255)
                                  aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCAGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGTTTGGAGATGACCTGCTGTAAACGCATCgg                                                                                                                                                                                                                                             >  2:411618/1‑275 (MQ=255)
                                     gagaCATCGCCGCCCATCATGCTTATCAGTTTCTCCCAAATCGCCATACTCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTc                                                                                                                                                                                                                                          <  2:124924/275‑1 (MQ=255)
                                      agctatCGCCCTCCATCATGCTGATCAGTTTTTCACAAACCGCCAGACCCAGACCGGTCCCCTGGAAATTCCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCAAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg                                                                                                                                                                                                                                         <  3:242132/272‑1 (MQ=255)
                                      agaTATCGCCGTCCCTCATGCTTATCAGTTGTTCACAAATCGCCAGGCCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg                                                                                                                                                                                                                                         <  3:218882/275‑1 (MQ=255)
                                       gaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                                                                                                                                                           >  3:430924/1‑272 (MQ=255)
                                            cGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAttt                                                                                                                                                                                                                                   >  3:513132/1‑275 (MQ=255)
                                             gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGGTGGAGATGACCTGCGGTAAACGCATCGGGTCGCCAtttt                                                                                                                                                                                                                                  >  3:501379/1‑274 (MQ=255)
                                                 tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACCCATCGGGTCGCCATTTAAAGcc                                                                                                                                                                                                                             >  2:269580/1‑276 (MQ=255)
                                                             atccatgtttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATGACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                  <  3:623035/268‑1 (MQ=255)
                                                               cAGTTTTTCACAAATCGCCCGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                               <  2:57042/276‑1 (MQ=255)
                                                               cAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGCCCTGCTGTAAACGTATCGGGTTCGCATTTAAGGCCACTGGccataccgg                                                                                                                                                                                                               >  4:279746/1‑268 (MQ=255)
                                                                atgttttCACAAATCCCCAGACCCAGACCGGTCCCCTGGGAATTACGCTGTCCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                               <  1:500853/272‑1 (MQ=255)
                                                                     ttCACAAAGCCGCAGACCCAGACCTGTCCCCTGGAATTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACGTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCTAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGCGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                          <  1:14862/275‑1 (MQ=255)
                                                                          cattccccaggcccggacCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGAGCAGACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                     <  3:196307/269‑1 (MQ=255)
                                                                          aacacGCCAGACCCAGATCGGTCCCCTGGAAATTACGCTGCACGCCCGTACAGACCTGGAAGAAGGGAGCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATGGCGAACACGGATCGAGAGGTAATCGCCATCCGAGCGAACATGCAAAACTATACAGCCGGTTTCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                     <  4:599155/271‑1 (MQ=255)
                                                                          aaaTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                     <  1:513132/275‑1 (MQ=255)
                                                                               gCCCGACCCAGACCGGTCCCCGGGAAACTACGCTGTACGCCCCTGCCGACCAGGAAGAAGGGCTCAAACCAGCG‑CACCACTTGTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAATATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  4:241040/276‑1 (MQ=255)
                                                                               gCCCGAACCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCTGTCCGAGCCGGAAGAAGGGATAAAACAAGCC‑GACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTa                                                                                                                                                                                                <  3:93894/275‑1 (MQ=255)
                                                                               cccacacccaggcCGGGCCCCCGGAAATTCCGCTCTACGCCCCGTCCGACCTGGAAGAAGTGATCAAACAAGCG‑CACCACTTCTTTCGCCGGGATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  2:592978/275‑1 (MQ=255)
                                                                                gcagacccagaccGGTCCCCTGGCAATTACGCGGTACGCCCGTTACGGCCTGGAAGAAGGGGTCAAACAAGCG‑ACCCACTTCTTTCGCCGGTAACCCCCCGCCGGGATCGCGAAAACGGATAGAGAGATAATCGCCATCCGCGCGAACATGGAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  3:460876/274‑1 (MQ=255)
                                                                                ccagacccagacCGGTCCCCTGGAACATACGCTGTACGCCCGTTCCGGCCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  2:279746/275‑1 (MQ=255)
                                                                                        agaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTa                                                                                                                                                                                        >  1:685461/1‑274 (MQ=255)
                                                                                            ccgTCCCCTGGAAACTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGc                                                                                                                                                                                   <  1:501379/273‑1 (MQ=255)
                                                                                               tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTTCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAAATCCGGTTCAATAAAGCAGTACAAGCCTAAATGCTTg                                                                                                                                                                                >  1:461579/1‑275 (MQ=255)
                                                                                               tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGAGT‑TACTGAACAGGTTGGAGATGAACTGCTGTAAACGCATCGGGTCGCCATTTAAGGACACTGGCACATCCGGTTCACACACGCAGGACAAGCCTAAcgcctttc                                                                                                                                                                               >  4:297572/1‑269 (MQ=255)
                                                                                                        cattcctccgaaCGCCCGTGCCGAACTGGAAGAAGGGATAAAACAAGCG‑CACCACTTCTTTCACAGGTATCCCCACGCCGGTATCGCGAACACGGATAGATAGAGAATCGCCATCCGCGCGAACATGCAAAACTATGCAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                      <  4:623514/265‑1 (MQ=255)
                                                                                                                  tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAg                                                                                                                                                                                                                                >  1:54364/1‑208 (MQ=255)
                                                                                                                  tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGca                                                                                                                                                                                                                      >  1:220510/1‑218 (MQ=255)
                                                                                                                  tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCCCATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAt                                                                                                                                                             >  1:250323/1‑275 (MQ=255)
                                                                                                                  tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAAc                                                                                                                                                                                      <  1:345430/250‑1 (MQ=255)
                                                                                                                  tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAAc                                                                                                                                                                                      >  3:345430/1‑250 (MQ=255)
                                                                                                                  tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGGATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAt                                                                                                                                                             >  1:136618/1‑275 (MQ=255)
                                                                                                                    cgccctgcccGACCTGAAAGAAGGG‑TCCAACCAGCGCCACCACGTCTTTCGACGGGATGCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                          <  4:153066/268‑1 (MQ=255)
                                                                                                                    cGCCCGGTCCGACCTGGAACAAGGGATCAAACAAGCG‑CCCCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCAAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACGAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                          <  4:68914/276‑1 (MQ=255)
                                                                                                                      cccGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                            <  3:54364/208‑1 (MQ=255)
                                                                                                                      cccGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATc                                                                                                                                                                                                                  <  3:220510/218‑1 (MQ=255)
                                                                                                                      accGTTCCGGCCTGGAAGAAAGGATCAAACAAGCG‑CACCACTTCTTTCCCCGGTATCCCCACGCCGGTATCGCGAACACGGATGGAGAGATAATCGCCATCCGCACGAACATGCAAAACTATACAGCCGGTATCTGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                          <  2:32560/273‑1 (MQ=255)
                                                                                                                          ttCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGTCACATCCGGTTCAATAAAGCAGTACACGCCTAACTCCTTTCGTACCACCAGCGGTAAATATTTGGCg                                                                                                                                                     >  3:162994/1‑275 (MQ=255)
                                                                                                                          ttCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGc                                                                                                                                                      >  3:674208/1‑274 (MQ=255)
                                                                                                                            ccGACCTGGAAGACGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCACGTTCAATAAAGCAGTCCAAGCCTAACTGCTTGCGTACACCCAGAGGGCAATATTTGTCgt                                                                                                                                                    >  3:306479/1‑273 (MQ=255)
                                                                                                                                              tCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCa                                                                                                                                >  4:513214/1‑276 (MQ=255)
                                                                                                                                                  aCAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcg                                                                                                                              <  3:685460/274‑1 (MQ=255)
                                                                                                                                                      gcg‑cACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACATGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTTGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTTGTTCCTCAATTCACGCGGTg                                                                                                                         >  1:354617/1‑275 (MQ=255)
                                                                                                                                                       cg‑cACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCCCATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCCAGCCGTGaa                                                                                                                       >  4:285185/1‑276 (MQ=255)
                                                                                                                                                        cggaccccTTCTTGCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTACACGCGGTg                                                                                                                         <  3:355578/271‑1 (MQ=255)
                                                                                                                                                                  cttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTa                                                                                                                                                                            >  1:110810/1‑213 (MQ=255)
                                                                                                                                                                     tccacgtccaccacACGCCGGGATACAGAACACGGAGTGAAGAATCATAGCCAGCCGCGCGAACATGCAAAACTATACAGCCGGTATCGTTGAATTTTAGGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCAGCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  4:508880/263‑1 (MQ=255)
                                                                                                                                                                      cGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                        <  3:110810/213‑1 (MQ=255)
                                                                                                                                                                      cGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  1:674209/275‑1 (MQ=255)
                                                                                                                                                                      cGCCGGGAACCCCACGCCGGTATCGCGAACACGGATAGGGGAATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  3:418161/275‑1 (MQ=255)
                                                                                                                                                                          gtacaccccAAGCCGGAAACGAGCAAAAGGATAGAGATGAAACCGCCATCCACGCAAAAGTGTAAAAATAAACAGCCGGTATCGGTGAAGTTTATCGAGG‑TACGGAACAGGTTGGAGATGACCTGCTATAAACGCATCGGGTCGACATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTANAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAACCGCACGCGGTTCGATc                                                                                                     <  4:348717/271‑1 (MQ=255)
                                                                                                                                                                            tATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTTGCGGTGATGTGGTTCATCACTTCACACGGTGAAAACTCACGCGGTACGATCt                                                                                                    >  3:575892/1‑275 (MQ=255)
                                                                                                                                                                               ccccACAACGGGATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAGACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCaa                                                                                                <  2:562496/276‑1 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGCCCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGCGAAAACTCACTCGGTTCGATCTTCAACTGTTCCGCTTCAAACTTCGCGAAATCGAGAat                                                               >  2:494834/1‑276 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGCTGTGGTTCATCACTT‑‑CGCGGTGAAACCTCACGCGGTTCGACCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGATaaa                                                               >  3:81435/1‑273 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCCCGCGGTGAAAACTCACGCGGGTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCACGAat                                                               >  4:513223/1‑276 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAACATCACCCGGTTCGATCTTCAACTGTTTCGATTCAATCTTAGAAAAATCGAGAat                                                               >  4:492889/1‑276 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCATTCTTCGACAAATCGAGaa                                                                >  3:474850/1‑275 (MQ=255)
                                                                                                                                                                                                                aTAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaa                                                                >  1:576623/1‑275 (MQ=255)
                                                                                                                                                                                                                  aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACCAGCCTAACTGCTTCCGTACCACCAGCGGTAACTAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGATAAATCGAGAat                                                               >  1:145510/1‑274 (MQ=255)
                                                                                                                                                                                                                      gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGc                                                          >  1:185549/1‑275 (MQ=255)
                                                                                                                                                                                                                          tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcgc                                                                                                              >  1:159508/1‑219 (MQ=255)
                                                                                                                                                                                                                          tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAAAAGACTAACTGCTTTCGTACCACCAGCGGTAAATAGTTGGCGGTGATGGGGTTCATCACTTCACGCGGTGAAAACTCACGCTGTTCGATCTTCAAATTTTCCGATTCAATCTTCGAGAAATCGAGaaaaatcatgat                                                      >  3:4225/1‑265 (MQ=255)
                                                                                                                                                                                                                              cgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTaaa                                                                                                                                                              >  3:90100/1‑167 (MQ=255)
                                                                                                                                                                                                                              cgcgAACATGAAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                          <  3:159508/219‑1 (MQ=255)
                                                                                                                                                                                                                                  aaCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGt                                                                                                                                                          <  1:90100/167‑1 (MQ=255)
                                                                                                                                                                                                                                     aTGCAAGACTATACATGCTGTTTCTGTGAATTTTATGGCGT‑TACTGAACAGGTTTGAGATGACCTGCTGTAACCGCATCGGTTCGCCATTTTAGGCCACTTGCACATACGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                          <  2:607898/276‑1 (MQ=255)
                                                                                                                                                                                                                                       gCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAacaaca                                        <  2:513214/276‑1 (MQ=255)
                                                                                                                                                                                                                                               atccgtccGGTTTTGTTGCATTTTAGTGCGT‑TACTTGACAGGTTGGAGATGACCTGCTTGAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGTGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGaa                                <  4:269580/270‑1 (MQ=255)
                                                                                                                                                                                                                                               ataCAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTgg                                  <  1:333494/274‑1 (MQ=255)
                                                                                                                                                                                                                                               agaCAGCCGGTATCTGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGaa                                <  2:297572/274‑1 (MQ=255)
                                                                                                                                                                                                                                                taCAGCCGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTTAAAACTCACTCGGTTCGATCTTCAACTGTTACGTTTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaccacaacgctgca                                 >  4:395525/1‑264 (MQ=255)
                                                                                                                                                                                                                                                  cggCCGCTAGCGGTGAATTTTATTGCGT‑TACTGAAAAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCAAATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATCTTCAACAACAGGCTGGAagag                             <  4:468653/274‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTg                                                        >  1:86551/1‑246 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgtt                           >  3:51493/1‑275 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgtt                           >  2:596797/1‑275 (MQ=255)
                                                                                                                                                                                                                                                     ccGGTATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATAGCTGATACTTTTCCACAACAGGCTGGAAGAgtt                           >  4:588459/1‑275 (MQ=255)
                                                                                                                                                                                                                                                         tATCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATaa                                                    <  3:86551/246‑1 (MQ=255)
                                                                                                                                                                                                                                                          aTCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTc                       >  1:123565/1‑274 (MQ=255)
                                                                                                                                                                                                                                                          aTCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATAGCTGATAATTTTCAACAACAGGCTGGAACAGTTGTTc                       >  1:443862/1‑274 (MQ=255)
                                                                                                                                                                                                                                                           tCGGTGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                               <  2:8362/105‑1 (MQ=255)
                                                                                                                                                                                                                                                               tGAATTTTATGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGGTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGCCTGGAAGAGTTGTTCATTGCCg                >  2:552961/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                        tGGCGT‑TACTGAACAGGTTGGAGATGACCTGCTGTCAACGCATCTGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATCTCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTTTTCATTGCCGTCACCat         >  1:42892/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                             t‑tACTGACCAGTTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTAACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCg  <  2:157347/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                             t‑tACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTACGATTCAATCTTCGAGCATTCGAGAATATCGCTGATCATTTTCAAAAACAGGCTGGAAGAGTTGTTCATTTCCGTCCTCAGCCGCTCg  >  4:66351/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                                aCTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                          >  4:126149/1‑234 (MQ=255)
                                                                                                                                                                                                                                                                                aCTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                          <  2:126149/234‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              
GGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCG‑CACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGT‑TACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCG  >  NC_000913/2317822‑2318365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A