Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F69 I1 R1 11 128.9 2630432 89.7% 2359497 260.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,318,095 C→G L594F (TTG→TTC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,318,0950CG97.0% 463.7 / NA 135L594F (TTG→TTC) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (61/69);  minor base T (3/1);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGA  >  NC_000913/2317820‑2318362
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                           
aCGGTAACCTGTCTGCCCATCCCCGGTTCTGAATCGACCGCGCTACCGCCGCCCATCAGGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                           <  4:42366/278‑1 (MQ=255)
   gTAACCTGGCTGCCCATGCCCGGTTCTGAATCTACCGGGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                           <  3:48388/275‑1 (MQ=255)
     aaaCTGGCTGCCCAATCCCGGGTCTGAAGATACCGAGATATAGCCGTCCATCATGCTGATCAGGTGTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg                                                                                                                                                                                                                                                                       <  4:109537/277‑1 (MQ=255)
        cTGGCTGCCCATACCCGGTTCTCAATCCACCGAGATACCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt                                                                                                                                                                                                                                                                      <  3:492815/275‑1 (MQ=255)
            cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCCCTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                           >  3:704014/1‑266 (MQ=255)
            cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGCACACGGTAAGAACGA‑TAAACGCCCTCCCCGCGAACACGCCAAACTCAACAGCCGGGCTCGTGTAATTTTATGTCCTTCCTTAACAGGTTGga                                                                                                                                                                                                                                                                  >  3:10929/1‑275 (MQ=255)
            cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACACACGGTATCGGTGAATTTTATGGCGCTCCTGAACAGGTTGGAGAt                                                                                                                                                                                                                                                               >  2:270532/1‑278 (MQ=255)
            cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAACCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCCCCATCCTCGCGAACCTGCAAAAATACACAACACACATAGCTGAATGTTATGGCCGTACTGAACCGGTGGgggaa                                                                                                                                                                                                                                                               >  4:497655/1‑277 (MQ=255)
            cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATCACGCTGTACGCCTGTTCCGACCTGGAAGAAGGGATC‑AACCAAGCGCACCACTTCTTTCGCCGGTTTCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCACAATCCGCGCGAACATGCAAAACTATACAGACGGTCACGGTCAATTTTATGGCGATAGTGAAAGGGTTggc                                                                                                                                                                                                                                                                  >  3:385907/1‑274 (MQ=255)
                ccATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg                                                                                                                                                                                                                                                                       <  1:704014/266‑1 (MQ=255)
                ccATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCATTACTGAACAGGTTGGAGATTAc                                                                                                                                                                                                                                                            >  2:210775/1‑277 (MQ=255)
                   ttCCCGGTTCTGAAACTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc                                                                                                                                                                                                                                                            <  1:160766/274‑1 (MQ=255)
                          ttCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTTGGGATGACCTGCTGt                                                                                                                                                                                                                                                     >  1:489463/1‑274 (MQ=255)
                                    aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCCAAACTATAAAGCCGGTATCGGTGAATTTGATGGCGTTACTGAACAGGTTGGAGATGACATGCTGTAAACGCATCGGGtg                                                                                                                                                                                                                                       >  2:244613/1‑277 (MQ=255)
                                    aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAAAGGTTGGAGATTACCTGCTGTAAACGCAACg                                                                                                                                                                                                                                           >  1:332489/1‑274 (MQ=255)
                                    aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAACGGTGAATTTTATGGCGTTACTTAACAGGTTGGATATGACCTGCTGTACACCCATCggg                                                                                                                                                                                                                                         >  4:342984/1‑276 (MQ=255)
                                     ccGAGATAGCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGt                                                                                                                                                                                                                                        <  2:342984/276‑1 (MQ=255)
                                        agaTATCCCCGTCCATCATGCGGATCCGTTTTTCCCCAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCg                                                                                                                                                                                                                                      <  3:672469/275‑1 (MQ=255)
                                                   tCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGACGGAATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCAGTATCGGTGAATTTTATGGCGTTACTGAACAGGTAGGAGATGACCTGCTGTAAACGCATCGGATCGCCATTTAAGGCCAc                                                                                                                                                                                                                        >  2:113096/1‑278 (MQ=255)
                                                    ccctcatGCTGATCAGTTTTTAACAAACCCCCAGACCCAGCCCGGGCCCCTGGAAATTACGCTGTACGCCCGTTACGACCTGGAAGAAGGGATC‑AAACAAGCGCCCCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGcc                                                                                                                                                                                                                          <  3:454367/272‑1 (MQ=255)
                                                     catcatGCTTATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGGACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                         <  1:3166/275‑1 (MQ=255)
                                                     catcatGCTGATCAGTTTTTCCCCAACCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGGACGCCCGTTCCGACCTGGAAGAAGGAATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa                                                                                                                                                                                                                         <  3:298285/275‑1 (MQ=255)
                                                                  aGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCGGGAAATTGCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAACGCACCACTTCTTTCGCCCGTATCCCCACGCCGGTATCCCGAACACGGATAGAGAGA‑TAATCGCCATCCGCTCGAACATGCAACACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCAATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                            <  1:700134/275‑1 (MQ=255)
                                                                       ttCACAAATCGCCACACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAAGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa                                                                                                                                                                                                       <  3:426352/275‑1 (MQ=255)
                                                                         cccaAACCGCCAGCCCCAGCCCGTTCCAGTGGAAATTACGCTGTCCGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCGGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                  <  2:147253/276‑1 (MQ=255)
                                                                            aaaTCGCCAGACCCAGCCCGGTCCCCTGGAAATTCCCCTGTACGCCCGTTCCGGCCTGGAAAAAGGGATC‑AAACAAGCGAACCACTTCTTTCGCCGGTAGCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                  <  3:392755/275‑1 (MQ=255)
                                                                             aagcGCCAGACACAGCCCGGCCCCCTGAAAATCACGCTGCACGTCCGAGTCGACCGGACATAAGGGGTA‑AAACAAGCGAACCACGTGTGTGCCCGGTATCCCCACGCCGGTGTCGCGAACACGGATAGAGAGA‑TAATGGCCAGCCGCGAGAACATGCAAAACTATACGTCCGGTATCGGTGAATTTTATGGCGTTACCGAGCAGATTGGAGAGAGCCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                  <  3:503272/271‑1 (MQ=255)
                                                                              aTCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATTGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATaaa                                                                                                                                                                                                   >  1:88376/1‑272 (MQ=255)
                                                                                cGCCCGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                            <  2:86558/277‑1 (MQ=255)
                                                                                cGCCAGACCCAGACCGGCCCAATGGAAATTACGCTGTACGCCCGATCCGACCTGGAAGAAGGGATC‑AAACGAGCGCACAGCAGCTTTCGCCGGTATCCCCACGCCGATATCGCGAACACGGATAGAGAGA‑TAGTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGGATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGt                                                                                                                                                                                              <  4:153082/275‑1 (MQ=255)
                                                                                  cccgacccagaccGTCCCCCAGGAAATTAAGCTGGACGCCCGTTCCGACCTGGAAGAAGGGAAC‑AACACAGCGCACCACTTCTTTCGCCGCTAGCCCCACGCCGGTATCGCGAAAACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                            <  3:91135/272‑1 (MQ=255)
                                                                                  ccagacccaggcCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGAACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                            <  3:169782/275‑1 (MQ=255)
                                                                                   cagacccagaacTGCTCCCTGGAAATTTCGCTATACGCCCGTTCCGACCTGGAAGAAGGGACC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc                                                                                                                                                                                            <  1:214640/274‑1 (MQ=255)
                                                                                                gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCACAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTTCg                                                                                                                                                                           >  2:247007/1‑278 (MQ=255)
                                                                                                gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGAGCGAAACTGCAACAATATAGAGCCGGTATCGGTGCATTTTAGGGGGGTCCTGAACAGGAGGGCGATTACGTGATGTCAACGCCGAGGGACCCCATTTAAGGCCACTGGCTCCTCTCGTTCAATAACTCAGTCCAATCCTAACTg                                                                                                                                                                                 >  3:701826/1‑272 (MQ=255)
                                                                                                      tGGAAAGTAGCCCGTACGCCCGTCACGACATCGGAGAAGGGAGC‑AAACAAGCGCACGACGTCTATCGCCGGTATCCCCACGCCGGTATCGAGAACACGGATAGAGAGA‑TAATCGCAATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac                                                                                                                                                                     <  4:270532/278‑1 (MQ=255)
                                                                                                        taaaTTACCCTGACCCCCCTTTCCGACCAGGAAGAAGGGATC‑AAACAAGCGCACAACTTCTTTCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                   <  4:246093/277‑1 (MQ=255)
                                                                                                         aaaTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGaa                                                                                                                                                                                                                                                                           <  3:410927/173‑1 (MQ=255)
                                                                                                           aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCTAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                   <  1:106332/275‑1 (MQ=255)
                                                                                                           aTTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca                                                                                                                                                                   >  1:730565/1‑275 (MQ=255)
                                                                                                                    tACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGAAACGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGAAGTACAAGCCGAACTGCTTGCTTACCACCAGCGGTAAAt                                                                                                                                                          >  1:455774/1‑275 (MQ=255)
                                                                                                                          cgtcacGACCTGGAAGGAGGGATC‑AAACAAGCGCACCTCTTCTGTCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGCGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTgg                                                                                                                                                    <  1:385907/270‑1 (MQ=255)
                                                                                                                            ttCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAAAGCCTCGGGTCGCCATTTAAGGCCACTGGCACATTCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAACAAGTTGGCg                                                                                                                                                  >  1:528069/1‑275 (MQ=255)
                                                                                                                            ttCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCCTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCACCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATGTTATGGCTTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCCTTTAAGTCACCTGGCACATCCGGGTCAATAAAGCAGTACAAGCCTCACTGATTGAGTACCACCAGCGGTAAATCGGTGGCggg                                                                                                                                                >  4:382319/1‑276 (MQ=255)
                                                                                                                            tcccGACCTGGAAAAAGGGACCAAAACAAGC‑AACCACTTCTTCCGCCGGTACCCCCCGGCCGGTATCGCGAACACGGTTAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACAATCCGGCCGGGATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg                                                                                                                                                  <  3:730565/273‑1 (MQ=255)
                                                                                                                             tccacccTGGAAGAAGGGATC‑AGACAAGCGCACCACTGCTTGCGCCGGTATCCCCACGCCGGTAACGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAGACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCgg                                                                                                                                                 <  1:37997/270‑1 (MQ=255)
                                                                                                                             tacGACCTTGAAGAAGGGATC‑AAAACAGCGCACCCCTTCTGTCGCCGGTATCCCCACGCCGGTATCGCGAACACGTATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGa                                                                                                                                              <  2:207378/276‑1 (MQ=255)
                                                                                                                              ccGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACATGCTGTAAACGCATCGGGTCGCCATTCAAGGCCACTGGCACATCAGGTTCAATCAAGCACAACACGCCTAACTGCTTGCGCACCCCTATCGGTAAATAGTCTGCGGTGa                                                                                                                                              >  2:153351/1‑277 (MQ=255)
                                                                                                                               cGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                            <  4:508872/214‑1 (MQ=255)
                                                                                                                                 aCCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtgt                                                                                                                                           >  2:498634/1‑277 (MQ=255)
                                                                                                                                     ggAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGTCCAATGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtgtg                                                                                                                                          >  1:559365/1‑274 (MQ=255)
                                                                                                                                      gaagaaGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGGACCACAAGCGGTCAATAGTTTGCGGTGATGTGTTtca                                                                                                                                     >  2:438711/1‑278 (MQ=255)
                                                                                                                                         gaagGGATC‑AAACAAACGACCCACTGTTTTCGCCGGTATCCCCACGCCGGTAACGCGAACACGGGTAGAGAGA‑TAATCGCGATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtca                                                                                                                                     <  3:482430/275‑1 (MQ=255)
                                                                                                                                                tC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCg                                                                                                                                                                                                             >  2:216420/1‑196 (MQ=255)
                                                                                                                                                tC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACtt                                                                                                                               >  1:240051/1‑274 (MQ=255)
                                                                                                                                                tC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcg                                                                                                                           <  2:362016/278‑1 (MQ=255)
                                                                                                                                                tC‑AAACAACCGCACCCCTTCTTTCGCCGGTACCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACGATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcg                                                                                                                           <  2:317536/278‑1 (MQ=255)
                                                                                                                                                 c‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                            <  4:216420/196‑1 (MQ=255)
                                                                                                                                                    aacaatgaccccacCTCGTACGGCGGTAACCCCACGCAGGTAGCGCGGACACGTGTGGGGAGA‑TAATCGCCCTCCACTCGAAAATGCAAAACTATACAGCCGGTATCGTGGAAGTTTATGGGTTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGTCCACTGGCACATCCGGTTCAATAAGGCAGTACAAGCCTAACTGCTTGCGGACCACCAGCGGTAAATAGTTAGCAGTGATGTGGTTCATCACTTcgcg                                                                                                                           <  3:377799/265‑1 (MQ=255)
                                                                                                                                                         gcgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtgt                                                                                                                                           >  4:267777/1‑254 (MQ=255)
                                                                                                                                                          gtcaccccTGCGTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCAcgc                                                                                                                          <  3:470694/268‑1 (MQ=255)
                                                                                                                                                          cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtgtg                                                                                                                                          <  2:267777/254‑1 (MQ=255)
                                                                                                                                                                    cttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTCCTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTTAATAATGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCTGTAAATAGTTGGCGTTGATGTGGTTCCTCACTTCTCGCGGTGAAACCTCACGCGGt                                                                                                        >  2:155924/1‑278 (MQ=255)
                                                                                                                                                                      ttCGACGGTATCCCCACGCAGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                       <  2:325285/277‑1 (MQ=255)
                                                                                                                                                                        ccccgggtcccccAAGCCGGTATCGCGAACCCGGAAAGA‑AGATTAATCGCCATCCCAGCAAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                       <  1:9865/266‑1 (MQ=255)
                                                                                                                                                                         tccGGTAACCCCAAGCCGGTATCGCGAACAAGGATAGGGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGACGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                       <  3:433443/273‑1 (MQ=255)
                                                                                                                                                                         gcagggataccaccGCCGGTATCGCTAACACGGATAGAGAGA‑TAAGCGCCATCCGCGCGAACATGCAAAACTAGACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                       <  3:240051/261‑1 (MQ=255)
                                                                                                                                                                             gTATCCCCACGCCGGTATCGCGAACACGGCTACAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGCTGGAGCTGCCCTGCTGTAAACGCATCGGGTCCCCATTTACGGCCACTCGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTCCTTCCGCACCACAAGCGGTACATCGTTGGCGTTGATGTGGTTTTTTCCTTCACGCTGTAAAACCTCACGCCGTTCgccc                                                                                                  >  3:390929/1‑272 (MQ=255)
                                                                                                                                                                             gTACCCCCACGCCGGTATCGCGAAAACGGGTAGGGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc                                                                                                  <  3:241420/275‑1 (MQ=255)
                                                                                                                                                                              taaccccACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCa                                                                                              >  2:281259/4‑278 (MQ=255)
                                                                                                                                                                                       gCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCg                                                                                     >  2:549631/1‑278 (MQ=255)
                                                                                                                                                                                                   aaCACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCg                                                                         >  2:419360/1‑278 (MQ=255)
                                                                                                                                                                                                                 ga‑TAAACGCCATCCGCACGAACATGCAAAACTATACAGCCGTTATCCGTGAATTTTATGGCGTTACTGAACAAGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCga                                                                <  3:332489/273‑1 (MQ=255)
                                                                                                                                                                                                                  a‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACACGGTTAAAACTCCCGCGGTTCGAGCTTACACTGTTCCGATTCACTCTTCAAGAACTAGAAAataa                                                          >  4:510691/1‑277 (MQ=255)
                                                                                                                                                                                                                  a‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTCGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAACCTACCTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGGTCATCACTTCATGCGGGGAAAACTCACGCGGTTCGATCTTCACTTGTTCCGATTCAATCTTCGACAAATCaagca                                                             >  3:278219/1‑273 (MQ=255)
                                                                                                                                                                                                                  a‑TAAACGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGTCGTAAATAGTTGGCGGCGATGTGGTTAATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTACATCTTCCAGAAATCGAGaa                                                             >  3:449437/1‑275 (MQ=255)
                                                                                                                                                                                                                    tAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACAt                                                                                                                                                                                                                >  1:157385/1‑127 (MQ=255)
                                                                                                                                                                                                                     aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTTAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTACGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACCCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGaaca                                                           >  1:405781/1‑273 (MQ=255)
                                                                                                                                                                                                                     aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCTGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTACATAGTTTGCGGTGATGTGGTTCATCACTTCACGCGGTGACAACTCCGGCGGTTCGATCTGCCACTGTTCCGATTCAATCTTCGAGAAATAGAGAataac                                                         >  2:53265/1‑275 (MQ=255)
                                                                                                                                                                                                                     aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAata                                                           >  1:515315/1‑275 (MQ=255)
                                                                                                                                                                                                                        cGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCgg                                                                                                                                                                                                            <  3:157385/127‑1 (MQ=255)
                                                                                                                                                                                                                                 cgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATa                                                  >  1:653938/1‑272 (MQ=255)
                                                                                                                                                                                                                                 cgcgAAAATGAAAAACTTTCAGGCCGGTTTCGGTGAATTTTAGGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTc                                            <  4:281259/278‑1 (MQ=255)
                                                                                                                                                                                                                                  gcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTTCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCCCTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAAttt                                              >  3:522216/1‑275 (MQ=255)
                                                                                                                                                                                                                                      aCATGCAAAACTATACAGCCGGTAGCGGTGATTTTTATGGCGTTACTGAACAGGTTGGGGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                       <  2:497655/278‑1 (MQ=255)
                                                                                                                                                                                                                                         tGCAAATCTGTACAGACGGGATCGGTAAAGTGTATGGCGTTACTGAACAGGTGGGAGATGACCTGCTGTAAAGGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAg                                    <  2:382319/278‑1 (MQ=255)
                                                                                                                                                                                                                                           cAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAAGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGGGAAATCGAGAATATCGCAGATAAgttgaaaaaaaagga                                  >  4:35176/1‑266 (MQ=255)
                                                                                                                                                                                                                                                      gcacGGTAGCGGTGAATTTGAGGGCGTTGCTGAACAGGGTGGAGGTGACCTGCGTTAAACGCATCGGGTGGCCATTTAAGGCCACTGGCACTGCCGGTTCAATAAAGCAGTACCAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGGTGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAagag                          <  3:559365/272‑1 (MQ=255)
                                                                                                                                                                                                                                                          ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAt                                                                                                                                                                                                      >  3:551884/1‑99 (MQ=255)
                                                                                                                                                                                                                                                          ggTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGGgttgt                      >  1:621986/1‑275 (MQ=255)
                                                                                                                                                                                                                                                           gTATCGGTGAATTTTCTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCCCATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATTATTTTTCACAACCGGATGGAAGCgttttt                     >  3:86263/1‑275 (MQ=255)
                                                                                                                                                                                                                                                           gTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTTTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGtt                                                                                        >  1:393485/1‑208 (MQ=255)
                                                                                                                                                                                                                                                              tCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg                                                                                                                                                                                                  <  1:551884/99‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCg             >  2:509714/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                  tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAcgc                                                                                                           >  3:278026/1‑182 (MQ=255)
                                                                                                                                                                                                                                                                  tGAATTTTATGGCGTTACTGAACAGGTTGGAGAAGACCAGCTGTAAACGCATCGGGTCGGCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGcc              <  1:367272/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aaTTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCCTCACTTCACGAGGTGAACACTCACGCGGATCGATCTTCAACTGTTCCGATTCAATCTTCGAGTATTCGAGAATACCGCTTAAAATTTTCAAAAAAAGTCGCGAAAAGTTGTTCATTTCCGTCat         >  4:418447/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                      ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGtt                                                                                                       <  1:278026/182‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCTGGTTCAATAAAGTAGTACAAGCCTAACTGCTTGCGTACCACCATCGGTAAATAGTTGGCGGTGATGTGTTTCACCACTTCACGCGGTGAAAACTCACGCGGTTCGCTCTTCAACTGTTCCGATTCAATCTTCGAGAAAACGAGAATATCGCTGATAATTTTCAACAACAGTCTCGAAGAGTTGTTCATTGCCGTCACCAg      >  1:672692/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                ttACTGAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCTATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCgaga                                                              >  1:220319/1‑213 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                           
ACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATC‑AAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGA‑TAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGA  >  NC_000913/2317820‑2318362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A