Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 26.7% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT26.7% 192.4 / 13.7 106intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (30/41);  new base T (20/9);  total (53/53)
Fisher's exact test for biased strand distribution p-value = 2.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  CP009273/2170575‑2170872
                                                                                                                                                       |                                                                                                                                                    
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑g                                                                                                                                                      <  2:4241735/150‑1 (MQ=255)
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑g                                                                                                                                                      <  1:4119311/150‑1 (MQ=255)
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑g                                                                                                                                                      <  1:2797883/150‑1 (MQ=255)
 ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Ga                                                                                                                                                     <  1:4276187/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                                    <  1:129485/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                                    <  1:129511/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                                    <  1:2285113/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                                    <  1:2285329/150‑1 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAatt                                                                                                                                                 <  2:1395856/150‑1 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTAttt                                                                                                                                             <  1:2901812/150‑1 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGt                                                                                                                                           <  2:2631416/150‑1 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGt                                                                                                                                           <  2:2630223/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGttt                                                                                                                                         <  1:2733062/150‑1 (MQ=255)
              tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGtttt                                                                                                                                        <  2:224996/150‑1 (MQ=255)
              tGCATCGTTTCGAGATTGAGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGtttt                                                                                                                                        <  2:224816/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGttttt                                                                                                                                       <  2:2715742/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑AAAAATATAAT‑GTAAATATtttttttt                                                                                                                                       >  2:3412478/1‑150 (MQ=255)
                 aTCGTTTCGAGATTGTTAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTaa                                                                                                                                     <  2:3556465/150‑1 (MQ=255)
                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTTATTT‑AAAAATATTAA‑TAAAtttttttttttttttt                                                                                                                                   >  1:4191188/1‑146 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAata                                                                                                                                  <  2:458690/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGACGTTTGTTTTAGATAT‑ATAAATATAAT‑GTAAATATATTTTTTTAAata                                                                                                                                  >  1:2013441/1‑150 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTAAAAAAAAAAATAAAAATTTTTTTTTTTTatatata                                                                                                                                >  1:605481/1‑149 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGGTTT‑AAAAAAAAAAA‑TAAAATATTTTTTTTTtaaaaaata                                                                                                                              >  1:605192/1‑144 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGTATTT‑AAAAAAATAAT‑AAAATTATTTTTTTTTTAaaatata                                                                                                                              >  1:605271/1‑147 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCgaga                                                                                                                            <  1:3252264/150‑1 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCgaga                                                                                                                            <  1:376262/150‑1 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑AAAAATATAAT‑GAAATTATTTGTTTTTTAATATAGAGATaa                                                                                                                         >  2:2360282/1‑150 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                        <  2:1445450/150‑1 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                        <  2:3836316/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                   <  1:3013024/150‑1 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                 <  2:857156/150‑1 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATcacc                                                                                                                 >  2:3131013/1‑149 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑AAAAATATAAT‑TAAAATATTTTTTTTTTAATATAGAGATAAAGAtaaaa                                                                                                                 >  2:3349841/1‑146 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATGTTCATATCCTGTCGTTTGTGTTAGATTT‑GAAAAAATAAA‑TAAATAAATTGTTTTTAAATATAGAGATGActaaaaaa                                                                                                                 >  2:3349832/1‑143 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                <  2:4040585/150‑1 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                <  1:2724442/150‑1 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑AAAAATATAAT‑GAAATTATTTTTTTTTATATTTCGAGATAACGGTCTCaa                                                                                                                >  2:2984818/1‑150 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGTTTTT‑AAAAATAAAAA‑TAAAATATTTTTTTTTTTATATATAGATAAACATCaaaaaa                                                                                                              >  1:1319328/1‑150 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTT‑AAAAATATAAA‑GAAAATATTTTTTTTTAAATATATAGATTACGATCACaaaa                                                                                                              >  1:2997265/1‑150 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTT‑CAAAAAAAAAT‑TAAATTATTTTTTTTTTTATATAGAGGGAAAGATCACaaaa                                                                                                              >  1:627522/1‑150 (MQ=255)
                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                            <  2:849488/150‑1 (MQ=255)
                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTT‑AAAAAAATAAT‑TAAAATATTTTTTTTTAAAAATAGAGATAAAAAACAaaaaaaa                                                                                                            >  2:3676275/1‑149 (MQ=255)
                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑TAAAATATAAT‑TAAAATATTTGTTTTTAAATATAGAGATAACCAACACAAAAAc                                                                                                            >  2:3676725/1‑150 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑AAAAATATAAT‑GAAAATATTTTTTTTTTAATATAGAGATAACGATCACAAAAACGACa                                                                                                        >  2:1729816/1‑150 (MQ=255)
                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑AAAAATATAAT‑GAAATTATTTGTTTTTTAATATCGAGATAACGATCACAAAAACGACaa                                                                                                       >  2:2692681/1‑150 (MQ=255)
                                                 aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGAGTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAata                                                                                                     >  2:3699414/1‑150 (MQ=255)
                                                   ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑CAAAATATAAT‑TAAATTATTTTTTTTTTAATATAGAGATAAAGATAACAAAAACGACAATATg                                                                                                   >  2:2348259/1‑150 (MQ=255)
                                                   ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑GAAAAAAAAAT‑TAAAATATTTTTTTTTTTAAATAGATATTAAGATCACAAAAAAGACaaaaag                                                                                                   >  1:653696/1‑146 (MQ=255)
                                                     gaaTAACCGCCGCGCTGTGCTTTGTTCAGCATATGATTTGTCGATACAACTTACATTTTCATATCCTGTCTTTTTTTTTCTATTT‑CAAAATATAAT‑TAAATTATTTGTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGaa                                                                                                 <  2:1404571/148‑1 (MQ=255)
                                                        tAACCGCCGCGCTGTGCTTTGTTCAGCATCTTCTTTGTCTATACCACTTACTTTTTAATATCCTGTCTTTTTTTTTCTATTT‑AAAAATATAAT‑GAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAat                                                                                              <  1:1160104/150‑1 (MQ=255)
                                                          cccgccgCGGTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACTTAAATTTTAATATCCTGTGGTTTGTTTTCTATTT‑AAAAATATAAT‑GAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAatta                                                                                            <  2:442312/149‑1 (MQ=255)
                                                          cccgccgCGCTGTGCGTTGTTCCGAATCTGCTTTGTCTATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCTATTA‑CAAAATATAAT‑GAATTTATTTTTTTTTAAATATGGAGATAACTAACACAAACACGACAATATGAAAatta                                                                                            <  2:442359/149‑1 (MQ=255)
                                                            cgccgcGCTGTGCGTTTTTCAGCTTCTGCTTTGTCGATACCACGTACATTTTAATATCGTGTCTTTTGTTTTCTATTA‑CAAAATTTAAT‑GAAATTATTTGTTTTTAAATATCGATATAACCATCACAAACACGACAATATGAAAattatt                                                                                          <  2:2452934/150‑1 (MQ=255)
                                                                  ggtgggCGTTGTTCAGCTTCTGGTTTGTCGATACCACGTACTTTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑TAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCgagga                                                                                    <  1:3131013/145‑1 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTT‑AAAAAAAAATT‑TTAATTATTTGTTTTTTAATATCGAGTTAACCATCTCAAAAAAGCCAAAAAGAAAATTATAAGAGGGGt                                                                                  >  2:829631/1‑150 (MQ=255)
                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGGTTT‑AAAAATAAAAT‑TAAAATATTTTTTTTTTAATATATTTATAAAAATAACAAAAAAAACAAAAATAAAATTATTAGTGGAGTGtaa                                                                              >  1:1736570/1‑150 (MQ=255)
                                                                               cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑GAAAATAAAAT‑TAAAATATTTTTTTTTAAATATAGAGAAAAACAAAACAAAAAACACCAAATGAAAAAAATTATAGGAGTGGAACGCaaaa                                                                       >  1:2696485/1‑150 (MQ=255)
                                                                                  cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTT‑TAAAAAAAAAT‑TAAAATAATTTTTTTTAAATAAAGAGATAAACAAAACAAAAAACACAAAATTAAAAATATTCTATGAGTGAAAGGCcaaaaaa                                                                    >  1:3161467/1‑150 (MQ=255)
                                                                                   ttCTGCTTTGTCTATACCACTTACTTTTTCATATCCTGTCGTTTTTTTTCGATTT‑CAAATTATATT‑GAATTTATTTGTTTTTAAATATCGATATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                   <  2:61746/149‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTT‑CAAAATATAAT‑CAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                   <  2:4222364/150‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTATTT‑AAAAAAATAAA‑AAAAATATTTTTTTTTAAAAATATAGTTAAAGAAAAAAAAAAAAAAAAAATTAAAAATATTCGAGGAGTGAAATGCaaaaaaaa                                                                   >  2:1637045/1‑149 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTT‑AAAAATATAAA‑TTAAATATTTTTTTTTAAAATACGAGATAAACAAAACAAAAAACAAAAAAATAAAAATAATATTGGCGTGAAAGGCcaaaaaaa                                                                   >  2:1637026/1‑149 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACATACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                   <  2:381923/150‑1 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGCTTT‑AAAAATATAAA‑TAAAATATTTTTTTTTTAAAATACTTATAAAAAAAACAAAAAAAACAAAAATAAAAAAATTAGAGGAGTGTAAGGCaaaaaaaac                                                                  >  2:459750/1‑148 (MQ=255)
                                                                                     cTGCTTTGTCCAAACAACTAACATTTTAATATCCTTTCTTTTTTTTTCGATTA‑AAAAATAAAAA‑GAAATTATTTTTTTTAAAATATCGAGAAAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                 <  2:478498/150‑1 (MQ=255)
                                                                                     cTGATTTTTCTATACCAATTAAATTTACATATCCTTTCGTTTTTTTTCTATTT‑AAAAATATAAT‑TAATTTATTTGTTTTTAAATATCTAGAAAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                 <  2:478563/150‑1 (MQ=255)
                                                                                             tCGAAACCACGTAAATTTTCATATCCTGTCGTTTTTTTTCTATTT‑CAAAATATAAT‑CAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                         <  2:3842212/150‑1 (MQ=255)
                                                                                                     ccttACTTTTTCATTGCGTGTGGTTTTTTTTCTATTA‑AAAAATATAAT‑GAATTTTTTTTTTTTTAAATATCGAGATACCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                 <  2:825411/147‑1 (MQ=255)
                                                                                                         aCATTTTCATATCCTGTCGTTTGTTTTGGATTT‑AAAAAAAAAAT‑TAAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAACGCAAATATGAAAATTATTCGAGGCGTGAAAGGCCAAAAAAAGGCCCCCCGCTTGGGCAGccct                                             >  2:3038998/1‑148 (MQ=255)
                                                                                                         aCATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑CAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                             <  2:201144/150‑1 (MQ=255)
                                                                                                         aCATTTTCATATCCTGTCGTGTTTTTTCGATTT‑CAAAATATAAT‑GAAAATATTTTTTTTTAAATATAGAGATAACCATCACAAAAAACCAAATATGAAAATTATTCGCGGAGTGTAAGGCAAAAAAACGGCCAACCCGGAGGGCAGCCGt                                             >  2:3038974/1‑150 (MQ=255)
                                                                                                              ttCATATCGTTTTTTTTGTTTTCTTTTT‑AAAAATATAAT‑TAATTTTTTTTTTTTTAAATATCTATATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                                        <  2:3585454/150‑1 (MQ=255)
                                                                                                                    tCCTGTCGTTTTTTTTAGATTT‑CAAAAAATAAT‑GAAATTATTTTTTTTTAAATATCGAGAAAACGAAAACAAAAAAGACAAAATTTAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCACCCGATAGGGAAGCCCTAGCAAAATgcg                                  >  2:701024/1‑150 (MQ=255)
                                                                                                                       tgtgtttttttttCTATTT‑AAAAATATATT‑TAATTTTTTTTTTTTTAATTTTCTATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                               <  2:1203182/145‑1 (MQ=255)
                                                                                                                       tGTCGTTTTTTTTCTATTT‑AAAAATATAAT‑TAAATTATTTTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                               <  2:3598053/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGttt                           >  2:4018226/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                           >  1:4137930/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                           >  2:4017671/1‑150 (MQ=255)
                                                                                                                                ttttCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                      >  1:2211302/1‑150 (MQ=255)
                                                                                                                                      aTTT‑CAAAATTTAAT‑GAAATTTTTTGTTTTTAAATATCGAGATAACGTTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                <  2:1732940/150‑1 (MQ=255)
                                                                                                                                       ttt‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               >  1:4538798/1‑150 (MQ=255)
                                                                                                                                       attaaaaaaaaTAAT‑GAAATTATTTTTTTTTAAATATCGAGATAACTAACACAACCACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                <  1:1729816/145‑1 (MQ=255)
                                                                                                                                        ttaaaaaaTATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGAACACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:479250/146‑1 (MQ=255)
                                                                                                                                           cAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  2:4243752/1‑150 (MQ=255)
                                                                                                                                            aaaaTATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt           >  1:4481061/1‑150 (MQ=255)
                                                                                                                                             aaaTATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg          >  2:320461/1‑150 (MQ=255)
                                                                                                                                             aaaTATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg          >  1:4142863/1‑150 (MQ=255)
                                                                                                                                             aaaTATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg          >  2:1083778/1‑150 (MQ=255)
                                                                                                                                              aaTATAAT‑TAAATTATTTGTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt         <  1:3086583/150‑1 (MQ=255)
                                                                                                                                               atatAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa        >  2:2714282/1‑150 (MQ=255)
                                                                                                                                               atatAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa        >  2:2714309/1‑150 (MQ=255)
                                                                                                                                                tataAT‑TAAATTATTTTTTATTAAATATCGACATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       <  2:3111094/150‑1 (MQ=255)
                                                                                                                                                  taat‑aaAATTTTTTTTTTTTAAATATCTATATACCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc     <  1:508648/150‑1 (MQ=255)
                                                                                                                                                  taAT‑TAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc     <  2:3319112/150‑1 (MQ=255)
                                                                                                                                                  taAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc     >  1:4528454/1‑150 (MQ=255)
                                                                                                                                                  taAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc     >  2:73961/1‑150 (MQ=255)
                                                                                                                                                   aaT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa    >  1:3610498/1‑150 (MQ=255)
                                                                                                                                                    aT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt   >  1:1909513/1‑150 (MQ=255)
                                                                                                                                                    aT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt   >  1:1909406/1‑150 (MQ=255)
                                                                                                                                                     t‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  1:1704288/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  CP009273/2170575‑2170872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A